Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc

8/3/2019 Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc

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Nghin cumts tc dngdc l cadchchitcy hon ngc

ti sinh vin NCKHChuyn ngnh: Ha sinh

Thchin: Sinh vin Phng ThHngLngHngdn: PGS.TS. LngThHng Vn


2/29

Ti liu chia s ti: wWw.SinhHoc.edu.vn t vn

Gc t do (GTD) l mt trong nhng nguyn nhngy ra s lo ha v rt nhiu cc loi bnh nh:ung th, vim khp, tim mch, i tho ng,nhimc

Cc GTD c thcloibnhcc cht ho hccbitcgi chung l cc chtchng oxy ho(COXH), chng c nhiu trong cc loi rau, c,qu v mt s cy thuc.

Cy HN vn c dn gian s dng nh mt cythuc c kh nng cha rt nhiu bnh khcnhau. Nhng mt s tc dng thc ca chng nhchng oxy ha, c ch mt s vi khun cncha c chng minh mt cch y .


3/29

Ti liu chia s ti: wWw.SinhHoc.edu.vnMc tiu nghin cu

1. Xc nh hm lng mt s cht c tc dngchng oxy ha trong cy Hon ngc

2. Xc nh kh nngkhng mt s vi khunc hi ca dch chit t l v r cy Honngc .


4/29

Ti liu chia s ti: wWw.SinhHoc.edu.vn Tng quan ti liu

1. Giithiuchungvcy HonNgc2. Gc tdo v nguyn nhn hnh thnh

gctdo3. Mtshnggiiquytgctdo4. icngvccchtchngoxy ho5. cimcamtschtchngoxy hothngcdng trongdcphm.


5/29

Ti liu chia s ti: wWw.SinhHoc.edu.vn I TNG NGHIN CU

itngnghin cuMt s b phn ca cyHN c nghin cu gmc:

+ L cy HN

+ R cy HN


6/29

Ti liu chia s ti: wWw.SinhHoc.edu.vnPHNG PHP, VT LIU NGHIN CU

1. Phng php nghin cu:S dngphng php thcnghim, phn tch, m t, so

snh cc mu c lp v sovi i chng.

2. Vt liu nghin cu

S dng 2 chng vi khun lEscherichia. coli ( E.coli )v Bacillus. subtilis (B.Subtilis) th hot tnhkhng sinh ca l v r cy

hon ngc.


7/29

Ti liu chia s ti: wWw.SinhHoc.edu.vn3. Ho cht:

Gm cc ho cht chnh nh:NaCl, Pepton, petrol ete, bubfer, ethanol, kali hidroxid, Natri

ascobat, methanol, - tocopherol, ascobic acid chun gc...

4. Thitbnghin cu:

+ My cc ph+ My sc k lnghiu cao nng (HPLC)+ Tm nui vi khun

+ Bung nui cy vi khun+ Cn phn tch+ Ni kh trng t+ V mt s thit b my mc khc


8/29

Ti liu chia s ti: wWw.SinhHoc.edu.vn

H thng my ccph H thng my sc klng hiu cao nng


9/29

Ti liu chia s ti: wWw.SinhHoc.edu.vnK thut tin hnh

K thut thu dch chit t l, r cy HN.K thut xc nh hot tnh khng khun

ca dch chit t cy HN.K thut xc nh hm lng vitamin E

bng my sc k lng hiu cao nng.K thut xc nh hm lng vitamin C

bng my cc ph.


10/29

Ti liu chia s ti: wWw.SinhHoc.edu.vnKt qu nghin cu1. Hm lng mt s cht COXH trong cy HN

Hm lng vitamin C trong cc mu dch chitm /k kh

Biu hm lng vitamin C trong cc mu dch chit

42.0854

0.88884.6478

0.071705

10152025

30354045

L ti L kh R ti R kh Tn mu

Hml

ngVT

MC

(mg/kgkh)

Hm lng


11/29

Ti liu chia s ti: wWw.SinhHoc.edu.vnBi u hm l- ng VTM E trong c c mu dch chit

53.0729

0 0

13.3977

0

10

20

30

40

5060

L t- i Rt- i Rkh L khM u

Hml-ngVTME(mg/

kg

kh

Hm l- ng

Hm lng vitamin E trong cc mu dch chit

(mg/kg kh)


12/29

Ti liu chia s ti: wWw.SinhHoc.edu.vn2. Kt qu th tc dng khng khun

nh chp mt mu thnghim th hot tnh khngkhun sau 30 gi nui cy.

Mu i chng (gia)Cc mu th dch chit

cy HN cc nng khc

nhau (xung quanh)


13/29

Ti liu chia s ti: wWw.SinhHoc.edu.vn2.1.So snh kh nng khng khun ca ccmu dch chit cy HN gia hai loi vi khun

Kh nng khng khun ca dch chit l ti honngc (chit bng nc ct) trn hai loi vi khun

0.7330.333

0.867

0.433

1.333

0.533

00.20.40.60.8

11.21.4d(cm)

0,125 0,25 0,5Nng (g/ml)

Vi khun B. subtilis.Vi khun E. coli

Biu so snh kh nng khng khun

cadch chit l ti cy HN chit bng ncct


14/29


Kh nng khng khun ca dch chit l ti HN(chit bng NaCl 0.9%) trn hai loi vi khun

Biu so snh kh nng khng khun ca dch chitl ti cy HN chit bng NaCl 0.9 %

d (cm)

0.4330.533 0.5

0.6

0.90.833

00.10.20.30.4

0.50.60.70.80.9

0,125 0,25 0,5Nng (g/ml)

Vi khun B. subtilisVi khun E. coli


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Kh nng khng khun cadch chit rti(chit bngnc ct) trn hai loi vi khun

Biu so snh kh nng khng khun ca dch chitr ti cy HN chit bng nc ct

0.5330.567

0.7330.7

0.967

0.767

00.10.20.30.40.50.60.70.80.9

1d(cm)

0,125 0,25 0,5

Nng (g/ml)

Vi khun B. subtilis.Vi khun E. coli. i


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Kh nng khng khun ca dch chit rti

(chit bng NaCl 0.9%) trn hai loi vi khun

Biu so snh kh nng khng khun ca dch chitr ti cy HN chit bng NaCl 0.9%

0.7

0.533

1

0.733

1.033

0.677

0

0.2

0.4

0.6

0.8

1

1.2d(cm)

0,125 0,25 0,5

Nng (g/ml)

Vi khun B. subtilis.

Vi khun E. coli.

l h h d

2 2 S h kh kh kh


17/29


Hot tnh khng vi khun B. subtilis ca dch

chit l v r ti cy HN chit bng nc ct

2.2. So snh kh nng khng khun ca cc mudch chit cy HN th trn B. subtilis

00.2

0.4

0.6

0.8

1d (cm)

Biu so s nh ho t tnh kh ng vi khun B.subtilis

ca dch chit l v rt- i cy HN chit bng n- c ctHLTHRT

0.125 0.25 0.5Nng (g/ml)

0.7330.533

0.867

0.733

1.333 0.967

Ti li hi i W Si hH d


18/29


Hot tnh khng vi khun B. subtilis ca dchchit l v r ti cy HN chit bng NaCl

0.9%

0

0.2

0.4

0.6

0.8

1d (cm)

Biu so s nh ho t tnh kh ng vi khun B.subtilisca dch chit l v rt- i cy HN chit bng NaCl

0.9% NLT

NRT

0.125 0.25 0.5Nng (g/ml)

0.433

0.7

0.5

10.9

1.033

Ti li hi t i W Si hH d

2 3 S h kh kh kh


19/29


Hot tnh khng vi khun E. coli ca dch chit l

v r ti cy HN chit bng nc ct

2.3. So snh kh nng khng khun ca cc mudch chit cy HN th trn E. coli

0

0.2

0.4

0.6

0.8

1d (cm)

Biu so s nh ho t tnh kh ng vi khun E.coli ca

dch chit l v rt- i cy HN chit bng n- c ct

HLTHRT

0.125 0.25 0.5 Nn /ml

0.333

0.5670.433

0.7 0.533

0.767



20/29


Hot tnh khng vi khun E. coli ca dchchit l v r ti cy HN chit bng NaCl

0.9%

0

0.2

0.4

0.6

0.8

1d (cm)

Biu so s nh ho t tnh kh ng vi khun E.coli cadch chit l v rt- i cy HN chit bng NaCl 0.9%

NLTNRT

0.125 0.25 0.5 Nng (g/ml)

0.533 0.533 0.60.677

0.8330.733


3 4 S h h t t h kh kh d h


21/29


Hot tnh khng vi khun B.subtilis ca dch

chit l ti cy HN gia hai dung mi khc nhau

3.4. So snh hot tnh khng khun ca cc mu dchchit khi chit cc dung mi khc nhau

00.2

0.4

0.60.8

1

1.2

1.4

d (cm)

Biu so s nh ho t tnh kh ng vi khun B.subtilis ca dchchit l t- i cy HN gia hai dung mi kh c nhau

n- c ct

NaCl 0.9%

0.733

0.433

0.867

0.5

1.333

0.9

Nng (g/ml)0.125 0.25 0.5



22/29


Hot tnh khng vi khun B.subtilis ca dchchit r ti cy HN gia hai dung mi khc

nhau

0

0.2

0.4

0.6

0.8

1

1.2d (cm)

Biu so s nh hot tnh kh ng vi khun B.subtilis cadch chit rt- i cy HN gia hai dung mi kh c nhau

n- c ct

NaCl 0.9%

0.533

0.7 0.733

1 0.967

1.033

Nng (g/ml)0.125 0.25 0.5



23/29


Hot tnh khng vi khun E. coli ca dchchit l ti cy HN gia hai dung mi khc

nhau

0

0.2

0.40.6

0.8

1

1.2d (cm)

Biu so s nh ho t tnh kh ng vi khun E.coli ca dchchit l t- i cy HN gia hai dung mi kh c nhau

n- c ct

NaCl 0.9%

0.3330.533 0.433

0.6

0.533

0.833

Nng (g/ml)0.125 0.25 0.5

Ti liu chia s ti: wWw SinhHoc edu vn


24/29


Hot tnh khng vi khun E. coli ca dchchit r ti cy HN gia hai dung mi khc

nhau

0

0.2

0.40.6

0.8

1

1.2d (cm)

Biu so s nh ho t tnh kh ng vi khun E.coli ca dchchit rt- i cy HN gia hai dung mi kh c nhau

n- c ct

NaCl 0.9%

0.5760.533

0.7 0.6770.767 0.733

Nn /ml0.125 0.25 0.5



25/29

Ti liu chia s ti: wWw.SinhHoc.edu.vnNhn xt chungCc kt qunghin cu ca chng ti cho thy:

+ Cc b phn ca cy HN c tc dng d-c l r rng bi n c kh nng c ch cc vikhun gy bnh v n c cha mt s chtCOXH.

+ Hm lng cc cht COXH trong l kh

cao nhng trong r th rt t.+ Cng t nghin cu ca chng ti chothy cy HN ch c tc dng dc l tt khi

cn ti. Khi sy kh th t c dng ca n


Kt l


26/29

Ti liu chia s ti: wWw.SinhHoc.edu.vnKt lun1. V hm lng cc cht COXH trong cy HN:

- Tt c cc mu l v r u cha vitamin C.

Trong , mu l c hm lng vitamin C cao hn rtnhiu so vi mu r; mu ti c hm lng cao hnnhiu so vi mu kh (P < 0.001).- Vitamin E ch c trong mu l (c ti v kh) vihm lng kh cao trong hm lng vitamin E l ti

cao hn rt nhiu so vi l kh, cn trong r (c tiv kh) khng tm thy vitamin E (P < 0.001).

2. V kh nng khng khun ca dch chitcy HN- Tt c cc mu ti (l v r) ca cy HN chitbng nc ct v NaCl 0,9% u c kh nng khnghai chng vi khun th nghim (B. subtilis vE.coli), cn i vi mu kh (l v r) u khng ckhnng khng hai chng vi khun th nghim ni

trn.



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Ti liu chia s ti: wWw.SinhHoc.edu.vn- Hot tnh khng B. subtilis ca cc mu dch chitcy HN ti c s khc nhau:+ Mu l ti chit bng nc ct c hot tnh cao hn so

vi mu l ti chit bng NaCl 0.9% v cao hn mu rti chit bng nc ct (p0.05).- Cc mu l ti chit bng nc ct v r ti chit bngNaCl 0.9% c hot tnh khng B. subtiliscao hn so viE. coli(P < 0.05).- Cc mu l ti chit bng NaCl 0.9% v r ti chitbng nc ct c hot tnh khng hai loi vi khun trn lnh nhau (P > 0.05).



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Ti liu chia s ti: wWw.SinhHoc.edu.vnKin ngh

Nn tip tc th hot tnh khng khun cacy HN vi cc chng vi khun khc.

Tip tc xc nh hm lng mt s thnh

phn ho sinh khc c kh nng COXH vkhng sinh trong cy HN nh selenium,carotenoit, flavonoit, alkaloit...

Th nghim tc dng COXH v khng khunca cy HN trn ng vt thc nghim


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Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc