Translation from Serbian language

UNIVERSITY OF BELGRADEFACULTY OF AGRICULTURE

Trademark of the FacultyBelgrade, April 8th

, 2013.Number. 33/111

Pursuant to the Law on plant nutrition products and soil improvers ("Official Gazetteof the Republic of Serbia" no. 41109) and based on Regulations on the conditions forclassification and determination of the plant nutrition products quality, deviations inthe content of nutrients and minimum and maximum values of allowed deviation innutrients and content of declarations and way of plant nutrition product marking("Official Gazette of the Republic of Serbia" no. 78/09) we hereby submit the

Report on tests performed for physical and chemical properties and biologicalnutritional value of fertilizers

1. General data from the request for entry into the register of plantnutrition products

1.1.Name of fertilizer: Organic onditioner - "GLISTENJAK EKO DAR"1.2.Name of the applicant: EKO DAR, Apatin, Rade Koncar st. no. 43,1.3.Name of the producer: EKO DAR, Apatin, Rade Konear st. no. 43,l.4.Fertilizer composition: Organic conditioner obtained by processing of

livestock manure by Californian earth worms with the following declaredquality parameters: total organic matter=min.40%, organic carbon-C=min.20%, ratio CIN=max.20, total nitrogen -N=min.l %, organicnitrogen=min.l %, total phosphorus-PiDe=min.I %, total potassium-K20=min.l%, humic acid=1,2%;

1.5.Purpose suggested: Glistenjak is aimed for improvement of physical, chemicaland microbiological characteristics of the soilwith lower content of organicmatter for growing of various plants of vegetable crops and horticulture, inquontities of 2-1 0 t/ha per year. It can be applied for improvement of variousorganic-mineral substrates in the quantity of 10-50 kg/rrr' of substratedepending on its supply of nutrient elements.

2. Results of the tests performed

- Classification of the ferilitzer for registration of plant nutrition product and soilImprovers:Organic soil improver, based on article 16 table 5.2. item 5 Rulebook on theconditions for classification and determination ofthe plant nutrition products quality,deviations in the content of nutrients and minimum and maximum values of alloweddeviation in nutrients and content of declarations and way of plant nutrition productmarking ("Official Gazette of the Republic of Serbia" no. 78/09)

2.1. Fertilizer chemical properties:- Average content of declared active substance from 3 repetitions:

- Total organic matter = 37,33%- Organic carbon - C= 18,41 %- CIN ratio=13,1- Total nitrogen-N=I,40%- Mineral nitrogen-Nf4-N=O,lO%- Organic nitrogen-N=I,30%- Total P20S =2,25%- Total K20=1,60%- Humic acid=2,00%

- Heavy metal content:

Pb - 15,84 mglkg;Ni - 15,34 mglkg;Cr - 18,65 mglkg;Cd - 0,05 mglkg;Hg - 0,01 mglkg.

2.2. Fertilizer physical properties:

- Appearance: homogenous pulverized organic matter, dark brown, odorless- Granulation: < 25 mm= 100%- Moisture:39,50%

3. Analytical testing methods

Rulebook on testing methods ("Official Gazette RS" no. 71/10)

- Organic matter: burning and applying live coal at 550°C- Humic acid: Dichromatic method, after extraction in 0,1 M KOH;- Total N: Kjeldahl method, upon destruction in H2S04 ;- Total P20S: Colorimetric method, upon destruction in H2S04;- Total K20: Flame photometric, upon destruction in H2S04;- Easily accessible NH4-N: Distillation from salt extract according to Bremner;-Heavy metals: AAS - method, by reading acetylene/air flame, after acid digestion

4. Application of fertilizer

4.1. Plant species and soil types for which it is intended: it can be used for variousplant species, especially for vegetable crop, lawns, flowers ornamental plantsgrown in different soil in the field and protected area, especially in soil withlower content of organic matter. It can be used for improvement of physical andbiological characteristics and feeding regime of various organic -mineralsubstrate.

4.2. Quantity applied: 2-10 tlha (0,2-1kg/m2) for soil and 10-50 kg/rrr' depending onits supply of nutrients.

4.3. Time of application: Before the basic or prior to seedtime preparation of soil andin preparation of substrate.

4.4. Way of application: Uniform distribution on the surface before the landcultivation and thorough mixing in substrate preparation.

4.5. Number of treatment per year: At one occasion.4.6. Possibile adverse effects: With suggested quantities and suggested way of

application there are no possibilities for adverse effects for plants grown.

5. Data on fertilizer nutritive value

Considerable content of total, specifically humified ortanic matter and lower contentofNPK biogenic elements can have a positive effect in improvement of physical,chemical and microbiological characteristics and feeding regime of soil. Determinedcontent of heavy metals is below the permitted.Due to well known biological value of this type of fertilizers obtained by naturalprocessing of organic material with Caliafornia earth worms additional testing byexperiment is not suggested.

6. Conclusion and suggestion for entry into register of plant nutrition products

Based on determined chemical and physical content pursuant to requirements of theapplicable Rulebook on the conditions for classification and determination of the plantnutrition products quality, the organic conditioner "GLISTENJAK EKO DAR" can beregistered for trade in our country, without previous testing of its biological value.This organic conditioner is intended for improvement of physical, chemical andmicrobiological characteristics and feeding regime of the soil for growing of variousplant species, especially for vegetable crop, lawns, flowers ornamental plants grownin different soil in the field and protected area, especially in soil with lower content oforganic matter, with annual quantity of2-1O tlha (0,2-1kg/m2) before the basic orprior to seedtime preparation of soil. Likewise it can be used for improvement ofvarious organic-mineral substrate in quantities of 10-50 kg/rrr', depending on itssupply of nutritients.Report made by:1. prof. Dragi Stevanovic, PhD.2. prof. Milica Kresovic, PhD.Print of round stamp of the University of Belgrade, Faculty of Agriculture.

The Dean:prof. Milica Petrovic, PhD

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Epoj: 33 _ /1,--,--11L-........--Shodno Zakonu 0 sredstvima za ishranu bilja i oplernenjivacima zemljista (SJ. gl.ti 41/09) i naosnovu Pravilnika 0 uslovima za razvrstavanje i utvrdivanje kvaliteta sredstava za ishranu bilja,odstupanjima sadrzaja hranljivih materija i minirnalnim i maksimalnim vrednostima dozvoljenogodstupanja sadrzaja hranljivih materija i 0 sadrzini deklaracije i nacinu obelezavanja sredstava zaishranu bilja (SI. gl. RS 78/09), podnosimo:

Izvestaj 0 izvrsenorn ispitivanju fizickih i bemijskih osobina i bioloske hranljive vrednostidubriva

1. Opsti podaci iz zahteva za upis u registar sredstava za ishranu bilja

1.1. Naziv dubriva: Organski oplemenjivac - »GLlSTENJAK EKO OAR«;1.2. Naziv podnosioca zahteva: EKO OAR, Apatin, Rade Koncara 43;1.3. Naziv proizvodaca: EKO DAR, Apatin, Rade Koncara 43;IA. Sastav dubriva: Organski oplemenjivac dobijen preradom stajnjaka sa kalifirnijskirn glistama,

u sledecirn deklarisanim parametrima kvaliteta: ukupna organska materija=minAO% organskiugljenik-C=min.20%, odnos C/N=max.20, ukupni azot-Nvmin.l %, organski azot=min.l %,ukupni fosfor-P20s=min.l %, ukupni kalijum-Kjfr=rnin.l ,0%. huminske kiseline=1,2%;

1.5. Namena koja se predlaie: Glistenjak je namenjen za popravku fizicko-hemijskih imikrobioloskih osobina zemljista sa manjim sadrzajern organske materije za gajenje razlicitihbiljnih vrsta u povrtarstvu i hortikulturi, sa kolicinorn od 2-10 t/ha godisnje. Moze seprimenjivati i za popravku razlicitih organo-mineralnih supstrata sa kolicinom 10- 50 kg 1 m3

supstrata u zavisnosti od njegove obezbedenosti hranjivim elementima.

2. RezuItati izvrsenih ispitivanja

- Klasifikacija dubriva za upis u Registar sredstava za ishranu bilja i oplernenjivaca zernljista :Organski oplemenjivac zemljista, na osnovu clana 16. tabela 5.2. tacka 5, Pravilnika 0

uslovima za razvrstavanje i utvrdivanje kvaliteta sredstava za ishranu bilja, odstupanjirnasadriaja hranljivih rnaterija i minimalnim imaksimalnim vrednostima dozvoljenog odstupanjasadrzaja hranljivih materija i 0 sadrzini deklaracije i nacinu obelezavanja sredstava za ishranubilja (Sl. gJ. RS 78/09).

Hevaisuua 6, flOllJTaHCKIl Ijlax l-l, 110&1 'lEMYH-bEOrPAJt - PErlYI:iJIHKA CP£HJATc.'l 1\101111: U~HTparra: (+ 3S 1-11) 2615-315; 31-60-191; ,L(cKtln.C··381-ll) 26 I2-(,l1·1; Te.~i!tll.1Kc:(~381-11 f2193-659

MnTlI'HlIl 6poj: r;7029845 rn15: 100 198~()2 Pn'IYHH: 840-1872660-97,8-10·\ 872666-7<lK-mail: officc@agrifl'g.ac.rs Web: www.agnf.bg.ac rs

2.1.Hemijske osobine dubriva:

I Prosecan sadrzaj deklarisane aktivne materije iz 3 ponavljanja:

- Ukupna organska materija=37,33%,- Organski ugljenik-C=18,41%,- Odnos CIN= 13,1,- Ukupni azot-N=I,40%,- Mineralni azot - NH4-N=0,1 0%,- Organski azot-N=l ,30%,- Ukupni P205=2,25%,- Ukupni K20= 1,60%,- Huminske kiseline=2,00%

- Sadrzaj teskih metala:

Pb - 15,84 mg/kg;Ni - 15,34 mg/kg;Cr - 18,65 mg/kgCd - 0,05 mg/kg;Hg - 0,01 mg/kg.

2.2. Fizicke osobine dubriva:Izgled: Homogena usitnjena organska materija, tarnno braon boje, bez mirisa.Granulacija: < 25 mm=! 00%.Vlaga: 39,50%.

3. Analiticke metode ispitivanja

Pravilnik 0 metodarna ispitivanja (Sg. RS br, 71/10)

Organska materija: Spaljivanjem i zarenjem na 550°CHuminske kiseline: Dihromatnom metodom, posle ekstrakcije u O,IM KOH;Ukupni N: Metodom po Kjedahl-u, nakon razaranja u H2S04;Ukupni P20S: Kolorimetrijski, nakon razaranja u H2S04;

Ukupni K20: Plamenfotometrijski, nakon razaranja II H2S04;

Lakopristupacni NH4-N: Destilacijom iz sonog ekstrakta po Bremmner-u;Teski metali: AAS - metodom, ciranjem u plarnenu acetilen/vazduh, nakonkiselinske digestije.

II

4.Primena dubriva

4.1.Biljne vrste i tip zemljista za koje je namenjeno: Moze se koristiti za razlicite biljne vrste,posebno za povrce, travnjake, cvece i ukrasno bilje, koje se gaje na razlicitirn zemljistima upolju i u zasticenom prostoru, posebno na zernljistima sa manjim sadrzajem organske materije.Moze se prirnenjivati i za popravku fizickih i bioloskih osobina i hranidbenog rezima razlicitihorgano-mineralnih supstrata.

4.2.KoliCina primene: 2-10 t/ha (0,2-1 kg/rrr') za zemljista i 10-50 kg I nr' supstrata u zavisnosti odnjegove obezbedenosti hranjivim elernentima.

4.3. Vreme primene: Pre osnovne ili predsetvene pripreme zemljista j u pripremi supstrata.4.4.Nacin primene: Ravnomerno rasturanje po povrsini pre obrade zernljista i dobrim mesanjem u

pripremi supstrata.4.5.Broj tretiranja u toku godine: U jednom navratu,4.6.Moguca nepovoljna delovanja: U predlozenim kolicinarna i predlozenim nacinirna primene ne

mogu se ispoljiti nepovoljna dejstva na gajene biljke.

5. Podaci 0 hranljivoj vrednosti dubriva

Znacajan sadrzaj ukupne, posebno humifikovane organske materije i manj i sadrzaj NPKbiogenih elemenata moze irnati pozitivne efekte u popravci fizicko - hemijskih i mikrobioloskihosobina i hranidbenog rezima zernljista. Utvrdeni sadrzaj stetnih teskih metala je ispod kolicinadozvoljcnog sadrzaja.

Zbog poznate bioloske vrednosti ove vrste dubriva dobijenc prirodnom preradom organskematerije sa kalifornijskim glistarna ne predlaze se njegova naknadna pro vera putem ogleda.

6. Zakljueak ipredlog za upis u registar sredstava za ishranu hilja

Na osnovu utvrdenog hemijskog i fizickog sastava saglasno zahtevima iz vazeceg Pravilnika 0

uslovima za razvrstavanje i utvrdivanje kvaliteta sredstava za ishranu bilja, organski oplernenjivac»GLISTENJAK EKO DAR« se moze registrovati za promet 1I nasoj zemlji, bez prethodneprovere njegove bioloske vrednosti.

Ovaj organski oplemenjivac jc narnenjen za popravku fizicko - hemijskih i mikrobioloskihosobina i hranidbenog rezima zernljista u gajenju razicitih biljnih vrsta, posebno za povrce,travnjake, cvece i ukrasno bilje, koje se gaje na razlicitim zernljistima u polju i u zasticenomprostoru, posebno na zemljistima sa manjim sadrzajem organske materije, sa godisnjom kolicinornod 2 - IQ t/ha (0,2- I kg/m2) pre osnovne ili predsetvene pripreme zemljista. Isto tako moze seprimenjivati i za popravku razlicitih organo-mineralnih supstrata sa kolicinorn 10 -- 50 kg / mJ

supstrata u zavisnosti od njegove obezbedenosti hranjivim elementima.

Izv~.tahsac~nili:, W-C/U--L-if-1. Pro. dr Dragi Stevanovic

2.. p~~ d~ji~'fn;:~VIC ,