Oncogenes & Tumor Suppressors - Cientisol · oncogenes are activated via point mutations, gene amplifications and gene translocations in a normal gene known as proto-oncogene and

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Antibodies for Characterizing Oncoproteins & Tumor Suppressor Proteins

Oncogenes & Tumor Suppressors

Oncogenes & Tumor Suppressors

Oncoproteins

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What are oncoproteins and how to characterize them?

Cancer is a complex disease that can be triggered by a variety of causes rangingfrom oncovirus infections to genetic mutations. Exposure to carcinogens and inheritance are two of the main sources for genetic mutations and predominantly occur in seven distinct protein groups (Lodish et al. 2000):

1. Apoptosis regulators2. Cell cycle control proteins3. Cell signaling proteins4. DNA repair proteins5. Growth factors6. Growth factor receptors7. Transcription factors

Mutations in growth factors, growth factor receptors, cell signaling proteins and transcription factors tend to result in dominantly active oncogenes; a term used to describe genes, which encode a cancer-promoting oncoprotein. Most oncogenes are activated via point mutations, gene amplifications and gene translocations in a normal gene known as proto-oncogene and result in altered gene expression or protein activity levels (Lodish et al. 2000).

Featured antibodies for the analysis of oncoproteins

Mutations in growth factors, growth factor receptors, cell cycle regulators, cell signaling proteins and transcription factors tend to result in dominantly active oncogenes, which alter the balance between cell proliferation and apoptosis.

The apoptosis regulator Bcl-2 oncoprotein plays an important role in deciding whether a cell lives or dies. It acts by controlling the permeability of the outer mitochondrial membrane. Murine anti-Bcl-2 antibody (clone 100) is frequently used in Immunohistochemistry experiments and has been successfully applied to demonstrate the presence of Bcl-2 in tissues of radioresistant squamous cell carcinoma (Condon et al. 2002). Western blot analysis of Bcl-2 is also frequently performed as part of a large apoptosis panel (including other markers such as Bak, Bax and Bcl-xL).

Western blot analysis of a Jurkat cell lysate with mouse anti-human Bcl-2 antibody (MCA1550) followed by detection with a goat anti-mouse HRP conjugated secondary antibody. Visualization was performed by chemiluminescence.

Applications: Flow Cytometry, Immunohistochemistry frozen and paraffin, Immunoprecipitation and Western Blotting

Citations: 11

Find out more at: abdserotec.com/bcl2

Featured murine anti-Bcl-2 antibody (MCA1550)

The Ras protein family encompasses three members HRAS, NRAS and KRAS. The genes encoding these proteins present some of the best studied proto-oncogenes, which upon mutation are involved in inducing various cancer types (including bladder, cervical, pancreatic and prostate cancers). Murine anti-H-Ras antibody (MCA2884) is frequently used in Western Blotting experiments and has been successfully applied for the detection of H-Ras in human epithelioid breast cancer cells (MCF10A) (Vuoriluoto et al. 2011).

Western blot analysis of a HEK293 cell lysate with mouse anti- human H-Ras antibody (MCA2884) followed by detection with a goat anti-mouse HRP conjugated secondary antibody. Visualization was performed by chemiluminescence.

Applications: ELISA and Western Blotting

Citations: 12

Find out more at: abdserotec.com/hras

Featured murine anti-H-Ras antibody (MCA2884)

Oncoproteins Oncoproteins - Cell Signaling Group

AbD Serotec offers a variety of antibodies and proteins for analyzing oncoproteins. For your convenience, we have grouped our oncoprotein research reagents into the four main oncoprotein classes (cell signaling proteins, growth factors, growth factor receptors and transcription factors).

Oncoproteins - Cell Signaling Group (Antibodies)

Find out more at: abdserotec.com/oncoabs 3

Specificity Product Type Clone Species Reactivity Applications Host Format Size Product Code

AKT1 Monoclonal Antibody 4C3 Human E Mouse Preservative

Free 0.1 mg MCA2919Z

AKT1 Monoclonal Antibody 6F11 Human, Mouse, Rat IF/ICC, WB Mouse Preservative


AKT1 Recombinant Antibody 2147 Human, Mouse, Rat E, WB HuCAL® Purified 0.1 mg HCA003

AKT1 Recombinant Antibody 3813 Human, Mouse, Rat E, WB HuCAL Purified 0.1 mg HCA017

AKT1 Polyclonal Antibody Human, Mouse, Rat WB Rabbit Purified 50 µg AHP968T

AKT1 Polyclonal Antibody Human, Mouse, Rat WB Rabbit Purified 0.1 mg AHP968

AKT2 Polyclonal Antibody 1F3 Human, Mouse, Rat E, IF/ICC, WB Mouse Preservative


AKT2 Polyclonal Antibody 1B3 Human IF/ICC, WB Mouse Preservative


AURORA-A Kinase

Monoclonal Antibody 35C1 Human, Mouse IF/ICC, WB Mouse Biotin 0.1 mg MCA2249B

AURORA-A Kinase

Monoclonal Antibody 35C1 Human, Mouse IF/ICC, IP, WB Mouse Purified 0.1 mg MCA2249

AURORA-A Kinase

Polyclonal Antibody Human IHC-F, IP Rabbit Purified 50 µg AHP873

AURORA-B Kinase

Polyclonal Antibody Human E, WB Rabbit Purified 50 µg AHP1261

Bax Monoclonal Antibody 1F5-1B7 Human IHC-P* Mouse Preservative


Bax Monoclonal Antibody 2D2 Bovine, Human,

Monkey E, IF/ICC, IP, WB Mouse Purified 0.1 mg MCA2738

Bax Polyclonal Antibody Human IHC-P*, IP, WB Rabbit Serum 0.1 ml AHP471

Bax Polyclonal Antibody Human IHC-P* Rabbit Serum 20 µl AHP471T

Bcl-2 Polyclonal Antibody Human E, WB Goat Purified 0.1 mg AHP1057

Bcl-2 Monoclonal Antibody 100 Human FC*, IHC-F, IHC-P*,

IP, WB Mouse Purified 0.1 mg MCA1550

Bcl-2 Polyclonal Antibody Human E Rabbit Purified 1 ml 0300-0016

Bcl-2 Polyclonal Antibody Human E Rabbit Purified 1 ml 0300-0020

Bcl-2 Binding Component 3

Polyclonal Antibody Human IHC-P*, WB Rabbit Purified 0.1 mg AHP727

Bcl-2 Binding Component 3

Polyclonal Antibody Human IHC-P*, WB Rabbit Purified 50 µg AHP727T

HRAS Monoclonal Antibody H-Ras-03 Human, Mouse, Rat E, WB Mouse Purified 0.1 mg MCA2884

KRAS Monoclonal Antibody 4F3 Human WB Mouse Preservative


KRAS Monoclonal Antibody 3B10-2F2 Human IF/ICC, WB Mouse Preservative


LCK Monoclonal Antibody LCK-01 Human FC*, IHC-F, IP, WB Mouse Purified 0.1 mg MCA2380

NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC* Mouse Alexa Fluor®

48825 Tests/ 0.25 ml MCA2410A488T

NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC* Mouse Alexa Fluor

488100 Tests/ 1 ml MCA2410A488

NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC* Mouse Alexa Fluor

647100 Tests/ 1 ml MCA2410A647

NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC* Mouse FITC 0.1 mg MCA2410F

NOTCH1 Monoclonal Antibody 4G1 Human, Mouse WB Mouse Preservative


Oncoproteins - Cell Signaling Group

Oncoproteins - Cell Signaling Group (Antibodies) Continued

Find out more at: abdserotec.com/oncoabs

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NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC*, IHC-F, WB Mouse Purified 0.1 mg MCA2410GA

NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC*, IHC-F, WB Mouse Purified 25 µg MCA2410T

NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC*, IHC-F, WB Mouse Purified 0.25 mg MCA2410

NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC* Mouse RPE 100 Tests MCA2410PE

NOTCH1 Monoclonal Antibody mN1A Human, Mouse FC* Mouse RPE 25 Tests MCA2410PET

NOTCH1 Monoclonal Antibody 8G10 Mouse, Rat FC, IHC-F, WB Hamster S/N 0.1 ml OBT2045

NOTCH2 Monoclonal Antibody HMN2-35 Mouse, Rat FC Hamster Purified 0.25 mg MCA5702

NOTCH2 Monoclonal Antibody HMN2-35 Mouse, Rat FC Hamster Purified 0.1 mg MCA5702GA

NOTCH2 Monoclonal Antibody HMN2-35 Mouse, Rat FC Hamster RPE 100 Tests MCA5702PE

NOTCH2 Polyclonal Antibody Human E, IHC-P* Rabbit Serum 50 µl AHP2180

NOTCH2 Polyclonal Antibody

Dog, Human, Mouse, Rat E, WB Rabbit Serum 50 µl AHP1289

NOTCH3 Monoclonal Antibody HMN3-133 Mouse, Rat FC Hamster Purified 0.25 mg MCA5703

NOTCH3 Monoclonal Antibody HMN3-133 Mouse, Rat FC Hamster Purified 0.1 mg MCA5703GA

NOTCH3 Polyclonal Antibody

Human, Monkey, Mouse, Rat E, IHC-P* Goat Purified 50 µg AHP2182

NOTCH4 Monoclonal Antibody HMN4-14 Mouse FC Hamster Purified 0.25 mg MCA5704

NOTCH4 Monoclonal Antibody HMN4-14 Mouse FC Hamster Purified 0.1 mg MCA5704GA

NOTCH4 Monoclonal Antibody HMN4-14 Mouse FC Hamster RPE 100 Tests MCA5704PE

PIM-1 Monoclonal Antibody 2C9 Human IHC-P Mouse Preservative


RAF-1 Monoclonal Antibody Roy/28/1 Human WB* Rat Purified 0.1 mg MCA2174

RAF-1 Polyclonal Antibody

Bovine, Chicken, Dog, Human, Primate WB* Rabbit Purified 0.1 ml AHP1237

RAF-1 pS338 Monoclonal Antibody DPR/2/2A/3A Human WB* Rat Purified 0.1 mg MCA1852

WNT-1 Polyclonal Antibody Human E, IHC-P*, WB Rabbit Purified 0.1 mg AHP1287

Oncoproteins - Cell Signaling Group Oncoproteins - Growth Factor Group

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Oncoproteins - Growth Factor Group (Antibodies & Proteins)



EGF Receptor Polyclonal Antibody Mouse E, WB Goat Purified 0.1 mg AAM52

EGF Receptor Polyclonal Antibody Rat E, FN, WB Rabbit Purified 0.1 mg AAR37

EGF Receptor Polyclonal Antibody Human E, FN, IHC-P*, WB Rabbit Purified 0.1 mg AHP767

EGF Receptor Polyclonal Antibody Human E, WB Rabbit Biotin 50 µg AHP767B

EGF Receptor Protein Mouse FN N/A Purified 1 mg EGF1

EGF Receptor Rec. Protein Human E, FN, WB N/A Rec. Protein 0.5 mg PHP030A

EGF Receptor Rec. Protein Mouse FN N/A Rec. Protein 0.5 mg PMP69

EGF Receptor Rec. Protein Rat FN N/A Rec. Protein 0.1 mg PRP31

FGF 4 Rec. Protein Human FN N/A Rec. Protein 25 µg PHP204

FGF 8 Rec. Protein Human FN N/A Rec. Protein 25 µg PHP130

FGF 9 Polyclonal Antibody Mouse E, WB Rabbit Biotin 50 µg AAM17B

FGF 9 Polyclonal Antibody Mouse E, FN, WB Rabbit Purified 0.1 mg AAM17G

FGF 9 Rec. Protein Mouse FN N/A Rec. Protein 10 µg PMP61

FGF 10 Polyclonal Antibody Human E, IHC-P*, WB Goat Purified 0.1 mg AHP1745

PDGF Monoclonal Antibody A04-5D9 Human E Mouse Purified 0.1 mg MCA5989GA

PDGF Polyclonal Antibody Human E, FN, IHC-P*, WB Rabbit Purified 0.1 mg 7460-0635

PDGF Polyclonal Antibody Human E, FN, IHC-P*, WB Rabbit Purified 0.1 mg AHP792

PDGF Polyclonal Antibody Human E, FN, IHC-P*, WB Rabbit Purified 0.1 mg AHP793

PDGF Rec. Protein Human E, FN, WB N/A Rec. Protein 10 µg PHP036

PDGF Rec. Protein Human FN N/A Rec. Protein 10 µg PHP035

VEGF Monoclonal Antibody 4B2-8 Human E, FN, WB Mouse Preservative


VEGF Monoclonal Antibody CH-10 Human, Mouse,

Rat WB Mouse Purified 0.1 mg MCA5693

VEGF Polyclonal Antibody Mouse E, FN, IF/ICC, IHC-P*,

WB Rabbit Purified 0.1 mg AAM51

VEGF Polyclonal Antibody Mouse E, WB Rabbit Biotin 50 µg AAM51B

VEGF Polyclonal Antibody Rat E, FN, WB Rabbit Purified 0.1 mg AAR42

VEGF Polyclonal Antibody Rat E, WB Rabbit Biotin 50 µg AAR42B

VEGF Polyclonal Antibody Human E, FN, IHC-P*, WB Goat Purified 0.1 mg AHP831

VEGF Polyclonal Antibody Human E, WB Goat Biotin 50 µg AHP831B

VEGF Rec. Protein Human E, FN, WB N/A Rec. Protein 10 µg PHP091

VEGF Rec. Protein Mouse E, FN, WB N/A Rec. Protein 10 µg PMP67

VEGF Rec. Protein Rat FN N/A Rec. Protein 10 µg PRP35

Oncoproteins - Growth Factor Receptor Group

Oncoproteins - Growth Factor Receptor Group (Antibodies)


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Specificity Product Type Clone SpeciesReactivity Applications Host Format Size Product Code

EGF Receptor Monoclonal Antibody ICR10 Human FC, IHC-F, IHC-P, IP Rat Purified 0.2 mg MCA1784

EGF Receptor Monoclonal Antibody ICR10 Human FC Rat FITC 0.1mg MCA1784F

EGF Receptor Monoclonal Antibody ICR10 Human FC, IHC-F, IHC-P, IP Rat Purified 1 mg MCA1784G

EGF Receptor Monoclonal Antibody ICR10 Human FC Rat RPE 100 Tests MCA1784PE

EGF Receptor Monoclonal Antibody F4 Human IHC-F* Mouse FITC 0.1 mg MCA455FT

EGF Receptor Monoclonal Antibody G02-2D5 Human E, WB Mouse Purified 0.1 mg MCA5986GA

EGF Receptor Polyclonal Antibody Human IHC-P* Rabbit Purified 50 µg AHP1372

EGF Receptor Monoclonal Antibody 1E4 Human E, FC Sheep Purified 0.5 mg MCA2198

EGF Receptor Monoclonal Antibody C1.1 Human E, FC Sheep Purified 0.5 mg MCA2199

EGF Receptor Monoclonal Antibody ICR11 Human FC, IHC-F, IP Rat Purified 0.2 mg MCA1785

EGF Receptor Monoclonal Antibody ICR11 Human FC, IHC-F, IP Rat Purified 1 mg MCA1785G

EGF Receptor Monoclonal Antibody ICR61 Human IHC-F*, IP Rat Purified 0.2 mg MCA1789

EGF Receptor Monoclonal Antibody ICR61 Human IHC-F*, IP Rat Purified 1 mg MCA1789G

EGF Receptor Polyclonal Antibody

Human, Mouse, Rat E, WB Rabbit Purified 50 µg AHP1243

EGF Receptor Monoclonal Antibody F4 Human IHC-F* Mouse S/N 2 ml MCA455

ErbB3 Polyclonal Antibody

Dog, Human, Mouse, Rat E, WB Goat Purified 0.1 mg AHP1088

ErbB3 Monoclonal Antibody SGP1 Human E, FC, IHC-F*, IP Mouse Purified 0.1 mg MCA1410

ErbB4 Polyclonal Antibody Human IHC-P* Rabbit Purified 50 µg AHP1131

HER2/neu Monoclonal Antibody ICR52 Human IHC-F Rat Purified 0.2 mg MCA1787

HER2/neu Monoclonal Antibody ICR52 Human IHC-F Rat Purified 25 µg MCA1787T

HER2/neu Monoclonal Antibody ICR55 Human FC, IHC-F, IHC-P,

IP, WB Rat Purified 0.2 mg MCA1788

HER2/neu Monoclonal Antibody ICR55 Human FC Rat Alexa Fluor

700 100 Tests MCA1788A700

HER2/neu Monoclonal Antibody ICR55 Human FC, IHC-F, IHC-P,

IP, WB Rat Purified 25 µg MCA1788T

HER2/neu Polyclonal Antibody Human E, IHC-F, WB* Rabbit Serum 0.1 ml AHP478

VEGF Receptor 1 Monoclonal Antibody FLT-19 Human E, IHC-F, IP Mouse S/N 0.1 mg MCA2743

VEGF Receptor 1 Polyclonal Antibody Human E, WB Rabbit Serum 0.1 ml AHP1600

VEGF Receptor 2 Polyclonal Antibody Human IHC-P* Rabbit Purified 50 µg AHP1327

VEGF Receptor 3 Monoclonal Antibody 5B5 Human WB Mouse Preservative


Oncoproteins - Growth Factor Receptor Group Oncoproteins - Transcription Factor Group

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Oncoproteins - Transcription Factor Group (Antibodies)

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Specificity Product Type Clone SpeciesReactivity Applications Host Format Size Product Code

c-Myc Monoclonal Antibody JAC6 Human IF/ICC, IHC-F, IP, WB Rat Purified 0.1 mg MCA1929

c-Myc Monoclonal Antibody 9E10 Human FC* Mouse Alexa Fluor

647 100 Tests MCA2200A647

c-Myc Monoclonal Antibody 9E10 Human E, IHC-F, IHC-P, WB* Mouse Alk. Phos. 0.1 mg MCA2200A

c-Myc Monoclonal Antibody 9E10 Human E, IHC-F, IHC-P, WB Mouse Biotin 0.1 mg MCA2200B

c-Myc Monoclonal Antibody 9E10 Human FC, IF Mouse Dylight® 549 0.1 mg MCA200D549GA

c-Myc Monoclonal Antibody 9E10 Human FC* Mouse FITC 0.1 mg MCA2200F

c-Myc Monoclonal Antibody 9E10 Human E, IHC-F, IHC-P, WB* Mouse HRP 0.1 mg MCA2200P

c-Myc Monoclonal Antibody 9E10 Human E, FC*, IHC-F, IHC-P,

WB* Mouse Purified 2 mg MCA2200G

c-Myc Monoclonal Antibody 9E10 Human FC* Mouse Alexa Fluor

488 100 Tests MCA2200A488


WB* Mouse Purified 1 mg MCA2200


WB* Mouse Purified 0.1 mg MCA2200GA


WB* Mouse Purified 20 µg MCA2200T

c-Myc Monoclonal Antibody 9E10 Human FC* Mouse RPE 100 Tests MCA2200PE

GL12 Polyclonal Antibody Mouse, Rat E, IHC-P*, WB Rabbit Purified 50 µg AHP1455

GL13 Polyclonal Antibody

Chicken, Dog, Human, Monkey, Mouse, Rat

E, IHC-P*, WB Rabbit Purified 50 µg AHP1456

HOXA11 Monoclonal Antibody 8B8 Human IHC-P, WB Mouse Preservative


LM02 Monoclonal Antibody 1A9-1 Human IF/ICC, IHC-P*, WB Mouse Purified 0.2 mg MCA2744

LM02 Monoclonal Antibody 1A9-1 Human IF/ICC, IHC-P*, WB Mouse Purified 0.1 mg MCA2744GA

PML Monoclonal Antibody 1D12 Human, Rat IF, WB Mouse Preservative


SMAD2 Monoclonal Antibody 3G9 Human IF/ICC, IHC-P, WB Mouse Preservative


SMAD2 Polyclonal Antibody Human WB Goat Purified 0.1 mg AHP694

SOX2 Monoclonal Antibody PR-4C6 Human E, WB Mouse Purified 0.1 mg MCA5659

SOX2 Monoclonal Antibody PR-4C6 Human E, WB Mouse Purified 25 µg MCA5659T

SOX2 Monoclonal Antibody PR-4E4 Human E, WB Mouse Purified 0.1 mg MCA5660

SOX2 Monoclonal Antibody PR-4E4 Human E, WB Mouse Purified 25 µg MCA5660T

SOX9 Monoclonal Antibody 3C10 Human IF, IHC-P, WB Mouse Preservative


v-myb/A-myb Monoclonal Antibody 5E11 Human, Mouse,

Xenopus IHC-F, IP, WB Mouse Purified 0.1 mg MCA1794

v-myb/c-myb Monoclonal Antibody 4E3 Xenopus IP, WB Mouse Purified 0.1 mg MCA1792

v-myb/c-myb Monoclonal Antibody 10A3 Human, Mouse,

Xenopus, Zebrafish IP, WB Mouse Purified 0.1 mg MCA1793

Tumor Suppressors

Mutations in two gene groups, tumor suppressors and oncogenes, play an important role in inducing and promoting a large variety of cancers. Oncogenes tend to encode growth factors, growth factor receptors, cell signaling proteins and transcription factors, while tumor suppressor genes encode cell cycle regulators. Most oncogenes are activated via point mutations, gene amplifications and translocations in a normal gene known as proto-oncogene and result in altered gene expression or protein activity levels (Lodish et al. 2000). In contrast to oncogenes, mutant tumor suppressor genes are recessive. They follow the Knudson or “multiple-hit hypothesis”, which suggest that both alleles of a gene need to be mutated in order to trigger disease onset.

In spite of this hypothesis, the inheritance of certain mutant tumor suppressor alleles, such as BRCA1 and BRCA2 mutant alleles, significantly increase the risk of developing breast and ovarian cancers. For example a female carrying a mutant BRCA1 allele has a 60-90% lifetime risk of developing breast cancer and a 40-60% risk of developing ovarian cancer (NHS UK). In light of these increased risks predictive genetic testing for BRCA1, BRCA2, TP53 and PTEN mutations are available for those who have a family history of early onset breast cancer.

Featured antibodiesThe human p53 protein is a well-studied protein consisting of transactivation (AA 1-94), DNA binding (AA 94-294) and oligomerization/tetramerization (AA 323-356) domains. The transactivation domain can be further divided into transactivation domain I (AA 1-43), transactivation domain II (AA 44-63) and a proline-rich region (AA 64-94).

AbD Serotec offers a variety of antibodies for the analysis of the different human p53 domains including:

Find out more at: abdserotec.com/suppressors

Mutations in tumor suppressor genes, such as TP53, and oncogenes such as H-Ras account for the development of a large variety of cancers.

Clone Name Binding region p53 domain Product Code

DO-2 AA 10-16 Transactivation MCA1702


BP53-12 AA 20-25 Transactivation OBT3175

PrecisionAb™ Monoclonal AA 20-25 Transactivation VMA00019K


Pab1801 AA 46-55 Transactivation MCA577


Pab246 AA 88-109 DNA binding MCA910

2C3 AA 94-202 DNA binding and oligomerization MCA3510Z

DO-11 AA 181-190 DNA binding MCA1704

Pab240 AA 213-217 DNA binding MCA909

FP3-2 pS392Oligomerization;Serine 392 becomes phosphorylated in response to UV damage

MCA1842

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Tumor Suppressors Tumor Suppressor Antibodies

The anti-p53 antibody (VMA00019K) is part of the PrecisionAb range and has been highly validated in Western Blotting. PrecisionAb products are characterized by their high specificity, high sensitivity and batch to batch consistency (every lot of antibody meets the same strict quality control criteria).

Find out more at: abdserotec.com/precisionab

Western blot analysis of whole cell lysates probed with anti-p53 antibody (VMA00019K) followed by detection with HRP conjugated goat anti-mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 2 second exposure. The arrow points to the p53 protein (molecular weight 53 kDa).

Featured PrecisionAbTM anti-p53 antibody (VMA00019K)

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BLM Polyclonal Antibody Human IP, WB Rabbit Purified 50 µg AHP875

BRCA1 Monoclonal Antibody MS110 Human

FC, IF/ICC, IHC-F*, IHC-P*, IP, WB

Mouse Purified 50 µg MCA1682GT

BRCA1 Monoclonal Antibody MS13 Human IF/ICC, IHC-P, IP,

WB Mouse Purified 50 µg MCA1683GT

BRCA1 Monoclonal Antibody BR64 Human IP, WB Mouse Purified 50 µg MCA5944GT

BRCA1 Monoclonal Antibody 6B4 Human IF/ICC, IHC-P, IP,

WB Mouse Purified 50 µg MCA5945GT

BRCA1 Monoclonal Antibody 17F8 Human E, IF/ICC, IHC-P,

IP, WB Mouse Purified 50 µg MCA5946GT

BRCA1 Monoclonal Antibody 8F7 Human IHC-F*, IHC-P*,

IP, WB Mouse Purified 50 µg MCA5947GT

BRCA1 Polyclonal Antibody Human IP, WB Rabbit Purified 50 µg AHP1470

BRCA2 Polyclonal Antibody

Cat, Dog, Human, Mouse, Rat IHC-F, IHC-P* Rabbit Purified 1 ml 0300-0023

Catenin beta Polyclonal Antibody

Dog, Human, Mouse

E, IB, IHC-F, IHC-P*, IP Rabbit Serum 50 µl OBT1683

Catenin beta Monoclonal Antibody 1C9 Human WB Mouse Preservative


Cdk2 Monoclonal Antibody 3148 Human E, WB HuCAL Purified 0.1 mg HCA063

Cdk2 Monoclonal Antibody 3148 Human E, WB HuCAL Purified 50 µg HCA063A

Cdk2 Polyclonal Antibody Human E, IHC-P, IP, WB Rabbit Serum 50 µl AHP1247

Cdk4 Monoclonal Antibody DCS-31.2 Human, Mouse, Rat IHC-F, IP, WB Mouse Purified 0.1 mg MCA1994

CDKN2A Polyclonal Antibody Human E, WB Goat Purified 0.1 mg AHP1060

CDKN2A Polyclonal Antibody Human IHC-P*, WB Rabbit Purified 0.1 mg AHP1488

Chk1 Polyclonal Antibody Human IP, WB Goat Purified 50 µg AHP877

Chk2 Monoclonal Antibody 4B7 Human IF/ICC,IHC-P*, WB Mouse Preservative


Cdk2 Polyclonal Antibody Human, Mouse, Rat E, WB* Rabbit Purified 50 µg AHP1266

Cyclin D1 Monoclonal Antibody CD1.1 Human, Rat E, FC*, IHC-F,

IHC-P*, IP, WB Mouse Purified 0.1 mg MCA1756

Cyclin D1 Monoclonal Antibody CD1.1 Human, Rat FC*, IHC-F, IHC-P* Mouse Purified 10 µg MCA1756T

Tumor Suppressors (Antibodies)

Find out more at: abdersotec.com/suppressorabs

Tumor Suppressor Antibodies

Tumor Suppressors (Antibodies) Continued

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Cyclin D1 Polyclonal Antibody

Dog, Human, Mouse, Rat E, WB Rabbit Serum 0.1 ml 2407-0508

Cyclin D3 Monoclonal Antibody DCS-22 Dog, Human IF/ICC IHC-F,

IHC-P*, WB Mouse S/N 1 ml MCA1866

Cyclin E Polyclonal Antibody Human, Mouse, Rat E, IP, WB Rabbit Serum 50 µl AHP1245

DNA PKcs Polyclonal Antibody

Hamster, Human, Mouse, Rat

GSA, IHC-P*, IP, WB Rabbit Serum 0.1 ml AHP318

NBS1 Polyclonal Antibody Human IP, WB Rabbit Purified 0.1 mg AHP842

p53 Polyclonal Antibody Human IHC-F, IHC-P* Rabbit Concentrate 0.1 ml AHP535T

p53 Polyclonal Antibody Human IHC-F, IHC-P* Rabbit Concentrate 1 ml AHP535

p53 Monoclonal Antibody BP53-12 Human FC* Mouse FITC 100 Tests OBT3175F

p53 Monoclonal Antibody 2C3 Human IF/ICC, IHC-P*,

WB Mouse Preservative Free 0.1 mg MCA3510Z

p53 Monoclonal Antibody PAb1801 Human IP, WB Mouse Purified 0.1 mg MCA577

p53 Monoclonal Antibody PAb240 Human IP, WB* Mouse Purified 0.1 mg MCA909

p53 Polyclonal Antibody Human, Monkey, Rat WB* Rabbit Purified 0.1 ml AHP906

p53 Polyclonal Antibody Human WB Rabbit Purified 50 µl 7045-3099

p53 Monoclonal Antibody DO-2 Human IHC-F, IHC-P*, IP,

WB Mouse Purified 0.1 mg MCA1702

p53 Monoclonal Antibody DO-2 Human IHC-F, IHC-P* Mouse Purified 20 µg MCA1702T



p53 Monoclonal Antibody DO-13 Human IHC-F, IHC-P* Mouse Purified 20 µg MCA1706T

p53 Monoclonal Antibody BP53-12 Human IHC-F, IHC-P*, WB Mouse Purified 0.1 mg OBT3175





p53 Monoclonal Antibody FP3-2 Human IHC-P*, IP, WB Mouse Purified 0.1 mg MCA1842

p53 Monoclonal Antibody DO-1 Bovine, Cat, Horse,

Human, Monkey IHC-F, IHC-P* Mouse Purified 10 µg MCA1701T


Human, MonkeyE, IHC-F, IHC-P*, IP, WB Mouse Purified 0.1 mg MCA1701


Human, Monkey FC* Mouse Biotin 0.1 mg MCA1701B


Human, Monkey FC* Mouse FITC 0.1 mg MCA1701F


Human, Monkey IHC-F, IHC-P* Mouse Ready to use 6 ml MCA1701H


Human, Monkey IHC-F, IHC-P* Mouse Ready to use 1 ml MCA1701HT


Human, Monkey FC* Mouse RPE 100 Tests MCA1701PE

p53 Monoclonal Antibody DO-7 Bovine, Human FC*, IHC-F,

IHC-P*, IP, WB Mouse Purified 0.1 mg MCA1703

p53 Monoclonal Antibody DO-7 Bovine, Human IHC-F, IHC-P* Mouse Ready to use 6 ml MCA1703H

p53 Monoclonal Antibody DO-7 Bovine, Human IHC-F, IHC-P* Mouse Ready to use 1 ml MCA1703HT

p53 Polyclonal Antibody Human IHC-F, IHC-P* Rabbit Ready to use 6 ml AHP535H

Find out more at: abdersotec.com/suppressorabs

Tumor Suppressor Antibodies Tumor Suppressor Antibodies

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Tumor Suppressors (Antibodies) Continued

References

Lodish H et al. (2000). Molecular Cell Biology, 4th edition. New York: WH Freeman; 2000.

Condon LT et al. (2002). Overexpression of Bcl-2 in squamous cell carcinoma of the larynx: a marker of radioresistance. Int J Cancer. 2002 Aug 1;100(4):472-5.

NHS UK. Predictive gene tests for cancer risk genes. http://www.nhs.uk/conditions/predictive-genetic-tests-cancer/Pag-es/Introduction.aspx, accessed June 08, 2015.

Vuoriluoto K et al. (2011). Vimentin regulates EMT induction by Slug and oncogenic H-Ras and migration by governing Axl expression in breast cancer. Oncogene. 2011 Mar 24;30(12):1436-48.

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PTEN Tumor Supressor Protein

Monoclonal Antibody A2b1 Human IHC-F*, IP, WB Mouse Purified 0.1 mg OBT1120

Retinoblastoma Monoclonal Antibody 7F10 Human, Mouse IHC-F, WB* Rat Purified 0.1 mg MCA2104

Retinoblastoma Monoclonal Antibody 51B7 Human, Mouse IHC-F, WB* Mouse Purified 0.1 mg MCA2105

Retinoblastoma Monoclonal Antibody IF8 Human IHC-F*, IHC-P*,

WB Mouse S/N 1 ml MCA1801

Retinoblastoma Monoclonal Antibody IF8 Human IHC-F*, IHC-P* Mouse S/N 0.1 ml MCA1801T

TSC1 Polyclonal Antibody Human, Mouse, Rat IHC-F, IHC-P, WB Rabbit Purified 50 µg AHP1001T

TSC1 Polyclonal Antibody Human, Mouse, Rat IHC-F, IHC-P, WB Rabbit Purified 0.1 mg AHP1001

WRN Monoclonal Antibody 3C11 Human, Rat IF/ICC, WB Mouse Preservative


WT1 Monoclonal Antibody 2H4 Human WB Mouse Preservative


XPA Polyclonal Antibody Human IHC-P*, WB Rabbit Serum 0.1 ml AHP452

*Requires special treatment refer to product datasheet. Abbreviations: Alk. Phos. = Alkaline Phosphatase, AA = Amino Acids, E = ELISA, FC = Flow Cytometry, FN = Functional Assay, GSA = Gel Supershift Assay,IF/ICC = Immunofluorescence/Immunocytochemistry, IHC-F = Immunohistochemistry - frozen (Cryostat), IHC-P = Immunohistochemistry - paraffin, IP = Immunoprecipitation, Pur. = Purified, Rec. = Recombinant, S/N = Supernatant, WB = Western Blotting.

Bio-Rad Laboratories, Inc.

LIT.OCT. V1. 2015 © Copyright Bio-Rad Laboratories, Inc. All rights reserved. Published by AbD Serotec, a Bio-Rad Company, Endeavour House, Langford Lane, Langford Business Park, Kidlington, OX5 1GE. AbD Serotec reagents are for research purposes only, not for therapeutic or diagnostic use. alamarBlue® is manufactured for AbD Serotec by Trek Diagnostic System. U.S. patent 5,501,959. Alexa Fluor® is a registered trademark of Molecular Probes Inc. OR, USA. DyLight® is a trademark of Thermo Fisher Scientific Inc, and its subsidaries. HuCAL® is a registered trademark of MorphoSys AG.

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Oncogenes & Tumor Suppressors - Cientisol · oncogenes are activated via point mutations, gene amplifications and gene translocations in a normal gene known as proto-oncogene and