ILA vaksine – forskningsnytt

Espen Rimstad, NMBU- Veterinærhøgskolen

Espen Rimstad, NMBU

• Efficient • Induce disease protection • Reduce/stop shedding • Long-lasting immunity • Administrated once

• Safe • No/little side effects • No/little environmental effects • No effect for consumer

• Low cost? • Easy to administrate

• Injectable versus oral or dip?

Ultimate fish vaccine – requirements?

Espen Rimstad, NMBU

• ViVaFish is a platform project funded by Research Council of Norway (RCN)

Primary institutions

National and international partners

Industrial partners

VIVAFISH Platform for Fish Virus Vaccines

Espen Rimstad, NMBU

http://www.google.no/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&docid=TiRAql-_cQMhMM&tbnid=yrhGdmMva--FDM:&ved=0CAUQjRw&url=http://www.haapihavet.com/program/bedriftsstand/&ei=BUt8U7W2J5T44QTR_IHABw&bvm=bv.67229260,d.bGE&psig=AFQjCNHlphEEatzJRjD4HhwFXq6QzKwL4w&ust=1400740997131426http://www.google.no/url?sa=i&source=images&cd=&cad=rja&uact=8&docid=bZd0VRXnvVewcM&tbnid=gYdaGtFxNYA-bM:&ved=0CAgQjRw&url=http://site.uit.no/fornybar/&ei=lkp8U9vbJeeO4gTbnIGgAQ&psig=AFQjCNHS10fKcYqJVEf2AEiJVul_TdXEfw&ust=1400740886695688http://www.google.no/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&docid=r9nLfYMyS7GtJM&tbnid=8cDi-kU3XXsKdM:&ved=0CAUQjRw&url=http://www.sas.upenn.edu/~marcmere/&ei=IFB8U-WWB9OL4gSkiIHABg&bvm=bv.67229260,d.bGE&psig=AFQjCNEAC8f0-QelCnDFGdjn5aIUhGpNMQ&ust=1400742301779447http://www.google.no/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&docid=rZq3oodRLA2wZM&tbnid=ONlt0t9PTsTFgM:&ved=0CAUQjRw&url=http://www.sjaweb.org/apps/pages/index.jsp?uREC_ID%3D181507%26type%3Dd%26pREC_ID%3D385735&ei=lFB8U-PzGafg4QTCiIHACw&psig=AFQjCNFIxP9LOiGemZOEBLkxcCORiprwaA&ust=1400742375772096

Whole virus and subunit preparations

Vectorized, propagation-defective

Live attenuated virus

VIVAFISH Platform for Fish Virus Vaccines

Vaccine concepts

Espen Rimstad, NMBU

Link between innate and adaptive immune

responses

Analyze adaptive CTL responses

B-cell responses involved in long term protection

VIVAFISH Platform for Fish Virus Vaccines

Immunology of long term protection

Espen Rimstad, NMBU

Optimization of delivery systems

Assays for long-lasting antiviral immunity

RNA seq / Array

VIVAFISH Platform for Fish Virus Vaccines

Espen Rimstad, NMBU

http://www.google.no/url?sa=i&rct=j&q=&esrc=s&frm=1&source=images&cd=&cad=rja&uact=8&docid=Zmjqewiuf-j6VM&tbnid=NaAWIE0BO0dUxM:&ved=0CAUQjRw&url=http://www.unil.ch/ib/page81028.html&ei=Wc1XU_ytF-uAywOEk4DoDw&bvm=bv.65177938,d.bGQ&psig=AFQjCNEVCFVAKAl-DwwltidMJZUnmvUvzw&ust=1398349141003370

Improved prototypes for vaccines

Vaccine delivery

response monitoring

Immunology of long term protection

Vaccine concepts

VIVAFISH Platform for Fish Virus Vaccines

Efficacy Safety In target species Dissemination Reversion Latency Ecotoxicity Target versus non-target species Host range. Establishment in the environment Potential for recombination

Espen Rimstad, NMBU

• Robertsen B, Chang CJ, Bratland L. IFN-adjuvanted DNA vaccine against infectious salmon anemia virus: Antibody kinetics and longevity of IFN expression. Fish Shellfish Immunol. 2016 Jul;54:328-32. doi: 10.1016/j.fsi.2016.04.027. PubMed PMID: 27108379.

• Caruffo M, Maturana C, Kambalapally S, Larenas J, Tobar JA. Protective oral vaccination against infectious salmon anaemia virus in Salmo salar. Fish Shellfish Immunol. 2016 Jul;54:54-9. doi: 10.1016/j.fsi.2016.03.009. PubMed PMID: 26994669.

• Tobar I, Arancibia S, Torres C, Vera V, Soto P, Carrasco C, Alvarado M, Neira E, Arcos S, Tobar JA. Successive Oral Immunizations Against Piscirickettsia Salmonis and Infectious Salmon Anemia Virus are Required to Maintain a Long-Term Protection in Farmed Salmonids. Front Immunol. 2015 May 27;6:244. doi: 10.3389/fimmu.2015.00244. PubMed PMID: 26074916; PubMed Central PMCID: PMC4445318.

• Chang CJ, Robertsen C, Sun B, Robertsen B. Protection of Atlantic salmon against virus infection by intramuscular injection of IFNc expression plasmid. Vaccine. 2014 Aug 6;32(36):4695-702. doi: 10.1016/j.vaccine.2014.05.059. PubMed PMID: 24996122.

• Wolf A, Hodneland K, Frost P, Hoeijmakers M, Rimstad E. Salmonid alphavirus-based replicon vaccine against infectious salmon anemia (ISA): impact of immunization route and interactions of the replicon vector. Fish Shellfish Immunol. 2014 Feb;36(2):383-92. doi: 10.1016/j.fsi.2013.12.018. PubMed PMID: 24374059.

• Wolf A, Hodneland K, Frost P, Braaen S, Rimstad E. A hemagglutinin-esterase-expressing salmonid alphavirus replicon protects Atlantic salmon (Salmo salar) against infectious salmon anemia (ISA). Vaccine. 2013 Jan 11;31(4):661-9. doi: 10.1016/j.vaccine.2012.11.045. PubMed PMID: 23200939

ILA vaksine

Espen Rimstad, NMBU

Espen Rimstad, NMBU

VIVAFISH Platform for Fish Virus Vaccines

Espen Rimstad, NMBU

VIVAFISH Platform for Fish Virus Vaccines

Chang et al 2014, Robertsen et al 2016

Pancreas disease

virus is an Alphavirus

Espen Rimstad, NMBU

VIVAFISH The use of PD virus as a vaccine vector

(Amplification+ dsRNA) CMV/T7 promoters

HH 5’UTR nSP1-2-3-4 Gene of interest 3’ UTR pA T7t

pnSP -SDV

J

IM injection to fish

nSP1234

Antigen expressed intracellularly

Ag presentation

MHC

Ab (IgM)

Cellular response T cyto.

Antigen

Espen Rimstad, NMBU

VIVAFISH The use of PD virus as a vaccine vector

(Amplification+ dsRNA) CMV/T7 promoters

HH 5’UTR nSP1-2-3-4 Gene of interest 3’ UTR pA T7t

pnSP -SDV

J

IM injection to fish

nSP1234

Antigen expressed intracellularly

Ag presentation

MHC

Ab (IgM)

Cellular response T cyto.

Antigen

Espen Rimstad, NMBU

VIVAFISH The use of PD virus as a vaccine vector

Espen Rimstad, NMBU

VIVAFISH Platform for Fish Virus Vaccines

Infeksiøs lakseanemi - ILA Wolf et al 2013, 2014

Espen Rimstad, NMBU

VIVAFISH Platform for Fish Virus Vaccines

Pankreas sykdom - PD Hikke et al 2014

Oral vaccines

Espen Rimstad, NMBU

Tobar et al 2015 –CentroVet (Chile)

Oral booster etter injeksjonsvaksiner gir økt mengde spesifikk IgM

Caruffo et al 2016,–CentroVet (Chile)

Oral vaksine alene gir en viss beskyttelse dersom den er pakket inn i spesielle mikrocapsuler.

• ISAV is a relative to Tilapia Lake virus.

Espen Rimstad, NMBU

ISA as a model?

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