1 Varian 940-LC Analytical to Prep HPLC. 2 Varian 940-LC Silver Bullets The 940-LC one instrument – many uses: –High performance automated Analytical

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<ul><li><p>Varian 940-LC Analytical to Prep HPLC</p></li><li><p>Varian 940-LC Silver BulletsThe 940-LC one instrument many uses:</p><p>High performance automated Analytical HPLC</p><p>High performance semi-preparative HPLC</p><p>High performance prep LC</p></li><li><p>Varian 940-LC Silver Bullets</p><p> Flexible and capable Wide flow rate from 0.1 ml/min to 200 ml/minDual path-length flowcells for extended AbsOne Injector, analytical or prep volumes (5ul to 4.5ml)</p><p>Superb fraction collectingGalaxie real-time peak detectionPractical collect and injectMuch Easier interface, it does what you tell it!Cleaner UI</p></li><li><p>Goals of the 940-LC Analytical to PrepAimBecome market leader in this $180M segmentDouble market shareMarketContinue to focus on Pharma / BioPharmaAcademicStrategyDeliver innovation in hardware, consumables, software and services </p></li><li><p>Varian 940-LC Instrument OverviewFactory tested, pre-plumbed instrument:Plug and play operationHigh performance semi-preparative HPLCGradient Analytical HPLCAutoprep SystemChoice of UV or PDA detectorDual path flow cell for unmatched range and scalabilityNo compromise performance for prep and analytical switch with a key stroke no plumbing or hardware changes needed!The most flexible analytical to prep HPLC available </p></li><li><p>Varian 940-LC Instrument OverviewScale-up Module for automated methods transfer from analytical to semi-prep, or switching from Analytical to Prep workVersatile and robust solvent-delivery systemNew loopless autosampler wide volume rangeNEW Fraction collection with Complete range of collection modes such as slope, threshold and time slicingWide range of vessels, including autosampler rackComprehensive range of columns and consumablesMore productivity via GalaxieTM softwareValve automation for genuine unattended operationNEW direct control interface</p></li><li><p>Varian 940-LC Versatility and PerformanceEasy to change pump headsVersatile solvent delivery systemsPowerful pumps allow 4000psi at 25mL/min operation for fast chromatographyIdeal for 4.6mm and 1 columnAble to use smaller 3 &amp; 5 um particles columns at elevated pressuresImproves productivitySave solvents and waste costsShort run times save timeHigh resolution separations = high purity1ml/minAnalytical21ml/minPrep</p></li><li><p>Varian 940-LC PumpsSame range of accessories as PS210/218</p><p>5ml Head to 200ml Head</p><p>Easily interchangeable pressure modules thru removable side panel.</p><p>Easy access to mixer</p><p>Self maintenance and versatility features have been completely retained with no compromises integrated systems are notoriously difficult to maintain so this is Unique</p></li><li><p>Varian 940-LC Pumps - FAB</p></li><li><p>Varian 940-LC AutosamplerPrep version of the 920-LC Sampler5ul to 4.5ml Injection rangeNo necessity to combine a Manual injectorNo need to dedicate injector to prep or analyticalOne Sampler does all!Cooled versionLoopless DesignNeedle in path DesignNo carryover2008 Technology no changing loops</p></li><li><p>Varian 940-LC Autosampler - FAB</p></li><li><p>Varian 940-LC UV/Vis - PDA DetectorsHigh Sensitivity for Analytical applications</p><p>Extended absorbance desensitised, for Prep Applications</p><p>All in One Cell!</p><p>NEW 4 x 0.25mm Cell low dead volumeExcellent performance at low flow rates for AnalyticalExcellent Extended Absorbance for Prep</p></li><li>Varian 940-LC Dual Pathlength FlowcellAbsorbance DetectorExtended absorbance range without re-plumbing (</li><li><p>Agilent 1200 Flowcells</p></li><li><p>Varian 940-LC UV/Vis - PDA Detectors - FAB</p></li><li><p>Varian 440-LC Fraction CollectorFully controlled by software for foolproof collection by slope, level or timesliceKnown fluid path for accurate fraction collectionSoftware display of collected fractions and source injections Reliable operationCompatible with a wide range of containersBelart and other optional racksMicrovial to 50ml tube capacityAutosampler rack compatibility provides practical collect and re-inject capabilities</p></li><li><p>Varian 440-LC Fraction CollectorWe chose not to collect Peak 3Peaks 1,2,4,5,6,7 collect with exceptional accuracyExcellent algorithm, and simple delay volume calculationSlope/Threshold based on real numbers!!</p></li><li><p>Varian 440-LC Fraction CollectorFraction Log shows peak location using colour coded rack assignment</p></li><li><p>Varian 440-LC Fraction Collector - FAB</p></li><li><p>Varian 445-LC Scale-up ModuleOptimized flow paths for automated scale-upSeparate optimised flow paths for Prep and Analytical columns Solvent selection valves for 4 solvents( 2 per pump)Integrated priming valves for easy solvent changesTwo Integrated Push Button Manual valves for Analytical and prep Injections</p></li><li><p>Varian 445-LC Scale Up Module( SUM) - FAB</p></li><li><p>Varian 940-LC Software UsabilitySoftware designed for Prep usersEnhanced manual controlOne page status and chromatogramReal time fraction collection displayAdvanced peak sensing for better fraction collectionPurity displays for absorbance detection standard!Documentation and audit trails for traceability and regulatory needs</p></li><li><p>Varian 940-LC Configurations</p></li><li><p>940-LC Pricing Market driven pricing</p><p> Similar pricing to existing systems at the same level</p><p> Opportunity to move system price up with valuable options</p><p> General move to higher ASP systems</p></li><li><p>Varian 940-LC ConsumablesColumns designed for Prep usersFrom 3 to 10mm mediaWide range of C4 to C18Silica and Polymer mediaFrom 2mm to 2 IDUnique solutions for dedicated prep with load and lock systemsMatched Analytical to Prep columns and packing's to assure smooth scale up Solutions for hardware, software and consumables</p></li><li><p>Applications of the 940-LC</p></li><li><p>Using Linear Scale-up Principles and the Varian 940-LC with Scale-Up Module for the Isolation of 5-Hydroxytryptophan from Griffonia Simplicifolia Dennis HoobinVarian Australia Pty Ltd679 Springvale Rd Mulgrave VIC Australia</p></li><li><p>Varian 940-LC Analytical to Prep Instrument</p></li><li><p>IntroductionGriffonia simplicifolia is a tall bushy vine found in Ghana, Nigeria, Liberia, Ivory Coast, Cameroon and Congo The vine produces pods containing 3 large legumesThe legumes are a rich source of 5-hydroxytryptophan (5HTP) 5-hydroxytryptophan produced by the human body from the essential amino acid L-hydroxytryptophan (LT)5-HTP has been clinically used to increase serotonin production to combat many mental illnesses like depression and schizophrenia among others</p></li><li><p>InstrumentationVarian 940-LC Semi-Prep HPLC (part no 02102201-00)Binary gradient pumps128 position autosampler with 5mL syringeScale Up Module to allow seamless scalability from Analytical to Semi-preparative applicationsOptimised Dual path flowcellDiode Array detectorAccurate Fraction collectionColumns - Varian XRs C18Analytical column -150 mm, 4.6 mm ID, 5u Preparative column - 150 mm, 21.2 mm ID, 5u </p></li><li><p>Instrument FeaturesScale Up ModuleFully Automated unit allowing switching between analytical and preparative columns software-controlled module switches from analytical to preparative operation to ensure that chromatograms are correlatedFlow celloptimized dual path flowcell that spans analytical to prep flow rates (125 mL/min) the dual path flowcell design provides the ability to work over an extended absorbance range (mAu to tens of Au)</p></li><li><p>Instrument ConditionsScaling UpScaleable System Elements Absolute System Elements Flow Rate Packing Material Tubing Area Column Length Sample Injection Volume Sample concentration Solvent Consumption Gradient Slope Run Time Back Pressure Flow Rate Packing Material </p></li><li><p>Instrument ConditionGradient conditionsMobile phase 85% A (H2O) 15% B (MeOH) Flow rates: 1 mL/min (analytical)21.2 mL/min (preparative)</p><p>Injection volume 50 L analytical4 mL preparative</p><p>Dual Array PDA detector: monitoring 2 channels at 230 and 280 nm.</p></li><li><p>Analytical to Preparative50 L analytical injection of 5HTP standard </p></li><li><p>Diode Array ChromatogramDiode array display. Quadrant 1: Chromatogram. Quadrant 2: 3D diode array data. Quadrant 3: Isogram. Quadrant 4: UV spectrum derived from chromatogram at cursor position </p></li><li><p>Instrument Scale up Chromatograms4 mL preparative injection of legume extract, full scale. Note the extended range absorbance maximum of 8.5 abs 50 L analytical injection of legume extract </p></li><li><p>Purity CheckFraction between 3 and 3.5 min in the preparative chromatogram was collected resulting in a fraction volume of 10.1 mLReinjection of the collected fraction onto the Analytical column results in a single peak chromatogram. Overlayed with the PDA peak purity trace, the flat top of the purity trace indicates a pure compound </p></li><li><p>DiscussionA good estimate of the amount of the % of HTP in dry Griffonia seeds can be madeThe entire 10 mL fraction contains 17.2 mg1gm of seeds contains 215mg of 5HTPApprox. 20% dry weightG. Simplicifolia seeds are a very rich source of 5HTP</p></li><li><p>ConclusionVarian 940-LC is a powerful automated system to allow easy development of HPLC preparative separationsDeveloping an analytical method seamless scale up to an optimised preparative columnsExcellent correlations between analytical and preparative chromatogramsConfidence in the purity of fractionated material</p></li><li><p>Varian 940-LC SummaryThe 940-LC one instrument many usesHigh performance automated Analytical HPLCHigh performance semi-preparative HPLCVersatilityPumping system able to operate at analytical flow rates and scalable up to prep flowsDual path-length flow cell technology for unmatched rangeScale-up Module for automated methods transferAdvanced fraction collection with full software controlComplete range of columns and consumablesPrep enhanced Galaxie TM software and valve automation for genuine unattended operation</p></li><li><p>Status of Varian HPLC at ReleaseInstallation/setup Perceptions Ease of ordering Operational flexibility Competitive performance Competitive specifications Ease of use Reliability Productivity Validation and Compliance Flexibility/expansibility (especially in semi-prep) Competitive pricing Wide product range hardware, columns, consumables </p></li><li><p>What About the Modular HPLC?</p></li><li><p>Modules OverviewWhat about modules?Low price manual, isocratic systemsAfter market module businessOdd configurations/applications not served by iHPLC Retaining210/218, SD-1 and 212 pumps325/335/356 detectorsCVM 500Still available but no need to actively sell due to iHPLC420 for Online SPE410/430Price Increases so beware!Yet, they will come!</p></li><li><p>Modules OverviewObsoleting220/230/240 ternary pump module340 UV detector355 RI detector363 Fluorescence detector400CVO module701 fraction collector</p></li><li><p>Varian 356-LC RI Detector</p></li><li><p>*Conclusion for 900 Series</p><p>Compact and Stylish Integrated Systems</p><p>Retain flexibility and maintenance features</p><p>Top Tier hardware performance</p><p>Top Tier User Interface pleasant experience</p><p>Simplicity in a box Install , Use and Maintain</p><p>1st Truly Integrated HPLCs on the Market</p></li><li><p>Questions??</p><p>Fast prep Integ fc co;\llectionPerformConsumablesAna to skidWe have uncovered the market need - *</p></li></ul>