VARIAN, INC. HPLC Products

Wide range of products in analytical LC• High performance analytical instrumentation

• Choice of workstations

• Extensive range of columns and consumables

• Strongest sales and support team

Excellent products and position in Preparative LC• Custom prep skid systems

Flexible instrumentation

Solutions for Biochemists and Biologists

Widest Range of Pumps Products in HPLC

Analytical Pumps• ProStar 210 Low cost isocratic analytical• ProStar 220 isocratic analytical • ProStar 230 ternary gradient analytical• ProStar 240 - quaternary gradient analytical

Preparative and Semi Preparative LC pumps• 210 - Low cost isocratic semi prep• PrepStar 218 - Low cost isocratic semi prep• PrepStar 210/218 binary and ternary gradients to

semi prep• PrepStar SD-1 - Prep and scale up• PrepStar SD-2 – Low cost prep pump

Widest Range of Detector Products in HPLC

UV-Vis detectors• ProStar 325 - high performance dual wavelength

analytical/prep

• ProStar 335 PDA detector

Other Detectors• ProStar 363 Fluorescence

• ProStar 355 RI

Widest Range of Sample Handling Products in HPLC

Autosamplers• 410 - High performance

• 420 - Flexible, high performance

• 430 - Well plate AutoSampler

Fraction collector• 701 - High performance, flexible

Widest Range of Prep Products in HPLC

500 CVM - Automated valving system

510 Column oven

530 Fluidics module

RamPak column packing stations

Lock and Load columns

Configured systems

Polaris – Low cost educational system

Widest Range of Software Products for HPLC

Galaxie – Client server multi PC, multi instrument network system

• Instrument Control, Data Handling, Custom reports

ReSponder software – single PC, 1 instrument• Simple graphical control for prep, data acquisition

but no data handling

ProStar 220/230/240 Solvent Delivery Systems

Ease of use•No need to degas or He sparge

•Simple pump priming

Reliability•Inlet valves do not fail

•Older designs have been working 20+ years

Performance•Gradient performance at all flow rates

ProStar 230/240 Solvent Delivery Systems

Mechanical Inlet Valve Design for improved flow delivery & reliability

ProStar 210/218 Solvent Delivery Systems

210/218 Pump head

Different sizes for different flow rates

Different materials

Optional piston wash

Different pressure ratings (dependent on pump type)

Pump Heads Available

Max flow Min flow

5 mL 10 µL

10 mL 10 µL

25 mL 25 µL

50 mL 50 µL

100 mL 100 µL

200 mL 200 µL

Features, Benefits and Values

Feature Benefit

Prep and analytical Lower cost

Built-in ADC Lower cost

Choice of materials Biocompatible

Low price isocratic Affordable

PrepStar SD-1 pump

PrepStar SD-1 Pump Design

Twin pump heads• Independently driven motor on each pump head• Independent pressure transducer on each pump head

controls flow independently• No pulsation throughout the entire flow range

– NO PULSE DAMPENERS REQUIRED• Solvent compressibility• Flow accuracy + 0.010 mL/min (0.1 mL/min for 2

largest heads)• Flow precision + 0.001 ml/min (0.01 ml/min for 2

largest heads)

PrepStar SD-1 Pump Benefits

• Choice of 5 pump heads covering range of flows– 50, 200, 500, 800 and 3200 ml/min

• Pump heads made of either stainless steel or titanium• Typical arrangement with 200 ml/min heads for best

accuracy in 0.01 ml/min up to 200 ml/min range– Ideal for analytical to prep scale up applications– Optimize separation using inexpensive small

diameter column at 1 ml/min– Switch valve to divert flow to preparative column

packed with identical material at 200 ml/min• Low pump pulsations increases column lifetime

PrepStar SD-2

PrepStar SD-2 Features and Benefits

Low cost system

Performance for real prep customers

Controlled by ReSponder Prep software

ProStar 325 Dual Wavelength UV-Vis Detector

Outstanding performance• Lowest noise of any dual wavelength

detector• Dual wavelength with ratio capability• Widest dynamic range from 50 AU to

70 AU• Handheld computer available to

monitor chromatogram in real time, create and edit methods

• Compatible with Galaxie and Star WS• Improved system monitoring

325 Optical design

ProStar 325 Controlled Optical Imaging

Unique Advantage of COI

•Minimize RI effects due to

–gradient– injection–solvent–temperature

325 Features and Benefits

COI• No RI response to temperature, solvent or flow

• Flatter baselines

• Better data handling results

Best noise specification

Excellent sensitivity

Flow cell - diagram

ProStar 335 Photodiode Array

Outstanding performance• Programmable slit widths 1, 2, 4, 8, 16

microns optimizes noise or resolution • Excellent low noise operation < 1 x 10-5

AU• Monitor all from 190 to 950 nm• Peak purity and spectral search available• Available in 2 versions

• Analytical (single array)• Prep (dual diode)

ProStar 335 PDA Optics

ProStar 335 Features and Benefits

ProStar 355 RI Detector

Outstanding performance• Active thermostating gives flatter

baselines

• Easy to use autozero improves analytical flexibility

• Auto purge for automated runs

• Wide dynamic range - 0.25 to 512x10-6 RIUFS

• Compatible with Galaxie and Star WS

ProStar 363 Fluorescence Detector

ProStar 363 Optics Diagram

ProStar 363 features and benefits

Feature Benefit

Reference diode Low noise

Intense lamp High signal

Scanning Find optimum wavelength

Computer control Easy to use

ProStar AutoSamplers

ProStar 410 – Most Common – 73%• Most common AutoSampler sold, 2 different

vial sizes, Prep injections, standalone reuse of standards

ProStar 430 – Bio – 17%• Well plate AutoSampler

ProStar 410 Autosampler

ProStar 430 Autosampler

Automation of 96 or 384 well-plates

ProStar 510 Column Valve Module

Outstanding flexibility• Valving for EVERY need

• Simple, neat handling of analytical and semi-prep columns

• Full software control

• Solvent compatibility check

• Compatible with Galaxie and Star WS

PrepStar 530 – Fluidics Module

PrepStar 530 capabilities

Interfaces the PrepStar SD-2 to ReSponder

Solvent selection for high pressure gradients• 4 A and 4 B solvents• Used with 2 pumps (SD-2, SD-1, PrepStar 218)

Solvent selection for low pressure gradients• Use 1 pump only, only SD-2• Form stepped and linear gradients.

Fraction collecting• 11 fractions• Unique valves for high flow rates• “Blow out” feature puts all sample into collection vial

PrepStar 530 capabilities

Recycle• Time program detector output back into pump and

column

Sample injection• Inject sample through pump

Detector input to ReSponder software

ProStar 701 Fraction collectors

RamPak Column Packer

Workstation

Star• Controls all mod and does full data handling

ReSponder – software for prep in the factory

Galaxie

Positioning the ProStar and PrepStar

Widest range of products in HPLC

Excellent products and position in Preparative HPLC

Custom prep skid systems

Choice of workstations

High performance analytical instrumentation

Flexible instrumentation

Solutions for Biochemists and Biologists

Full line of prep products - 1

210 pump with larger heads• Occasional semi prep

218 pump with larger heads 200 mL/min• Routine semi prep

SD-1 pump• Scale-up to prep

SD-2 pump• Bench top prep

325 detector• Dual path for extended range• Super prep flow cell

335 PDA with prep flow cell• Diode array prep capability

Full line of prep products - 2

701 Fraction collector• Flexible, controlled by the Galaxie workstation

500 CVM with prep and diverter valves• Occasional fraction collecting capability – lower flows

530 Fluidics module• Low pressure gradient with SD-2

• Solvent selector

• Fraction collector

• Sample injection

• Recycle

Full line of prep products -3

410 AutoSampler• Prep injection capability

Injection with 210/218 pump - AutoPrep• Inject any volume

Peak tracking software in Star• Complete package for prep

ReSponder software• Simple prep software• Control of the PrepStar 530

RamPak column packing stationsLoad and Lock columns

Custom large prep systems

Up to 10 L/min

Up to 16 in. diameter columns

Custom designed and installed PCG ( Prep Chromatography Group) in Wakefield

Flow Cell - Dual optical path

9mm and 1 mm

4 mm and 0.15 mm super prep cell

Extends linear range of the detector to greater than 10 AU

Automatic switching between the two paths at high absorbance