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Unit 1: The Mystery #2
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Careers Grab Bag Restriction Enzymes
Gel Electrophoresis
Confidentiality
QuestionAnswer
Careers-100
• ANSWER: The individual performs DNA profiling.
• QUESTION: Who is a DNA analyst?
QuestionAnswer
Careers-200
• ANSWER: A physician who performs an autopsy when death may be accidental or violent.
• QUESTION: Who is a medical examiner?
QuestionAnswer
Careers-300
• ANSWER: This individual performs scientific tests on bodily fluids and tissue samples to identify any drugs or chemicals present in the body.
• QUESTION: Who is a toxicologist?
QuestionAnswer
Careers-400
• ANSWER: This individual provides medical treatment at the scene of a crime or on the way to a hospital.
• QUESTION: Who is an emergency medical technician (EMT)?
QuestionAnswer
Careers-500
• ANSWER: This individual pronounces death and signs the death certificate.
• QUESTION: Who is a coroner?
QuestionAnswer
• ANSWER: This person is responsible for notifying the appropriate responders.
• QUESTION: What is a 911 operator?
Grab Bag-100
QuestionAnswer
Grab Bag-200
• ANSWER: Demonstrating reliability, give credit to the author, as a source for additional information.
• QUESTION: What are reasons to document (cite) your sources?
QuestionAnswer
Grab Bag-300
• ANSWER: Larger pieces of DNA will travel _____________ than small ones in a gel.
• QUESTION: What is slower?
QuestionAnswer
Grab Bag-400
• ANSWER: To help determine sizes of unknown DNA fragments.
• QUESTION: Why do scientists load DNA of known sizes into the agarose gel?
QuestionAnswer
Grab Bag-500
• ANSWER: This is the name given to the morgue assistant that helps prep the body for autopsy.
• QUESTION: What is the diener?
QuestionAnswer
Restriction Enzymes-100
• ANSWER: A degradative enzyme that recognizes specific nucleotide sequences and cuts up DNA.
• QUESTION: What is a restriction enzyme?
QuestionAnswer
Restriction Enzymes-200• ANSWER: Differences in DNA sequence
on homologous chromosomes that can result in different patterns of restriction fragment lengths (DNA segments resulting from treatment with restriction enzymes).
• QUESTION: What are Restriction Fragment Length Polymorphisms (RFLPs)?
QuestionAnswer
Restriction Enzymes-300
• ANSWER: Segments of DNA cuts with restriction enzymes are measured in number of this.
• QUESTION: What is number of base pairs?
QuestionAnswer
Restriction Enzymes-400
• ANSWER: Where restriction enzymes are found.
• QUESTION: What is bacteria?
QuestionAnswer
Restriction Enzymes-500
• ANSWER: The restriction enzyme EcoR1 cuts a strand of DNA at four restriction sites resulting in this many RFLPs.
• QUESTION: What are five?
QuestionAnswer
Gel Electrophoresis-100
• ANSWER: In gel electrophoresis, DNA shows up as these on the gel.
• QUESTION: What are bands?
QuestionAnswer
Gel Electrophoresis-200
• ANSWER: In gel electrophoresis, DNA is fragments are separated based on this.
• QUESTION: What is number of base pairs?
QuestionAnswer
Gel Electrophoresis-300
• ANSWER: During gel electrophoresis, this causes DNA to move out of the wells and through the gel.
• QUESTION: What is the attraction of the negative DNA, from the phosphate groups, to the positive electrode?
QuestionAnswer
Gel Electrophoresis-400
• ANSWER: During gel electrophoresis, this size fragments migrate further in the gel due to less friction with the agarose.
• QUESTION: What are smaller fragments?
QuestionAnswer
Gel Electrophoresis-500
• ANSWER: This is added to a gel to separate different size fragments on DNA.
• QUESTION: What is an electric current?
QuestionAnswer
Confidentiality-100
• ANSWER: A comprehensive set of standards and practices designed to give patients specific rights regarding their personal health information.
• QUESTION: What is the Health Insurance Portability and Accountability Act (HIPAA)?
QuestionAnswer
Confidentiality-200
• ANSWER: Individually identifiable health information such as demographic data, mental/health condition, and payment information is considered this.
• QUESTION: What is protected health information (PHI)?
QuestionAnswer
Confidentiality-300
• ANSWER: It is ok to do this when trying to prevent child abuse, domestic violence, or the outbreak of a disease.
• QUESTION: What is break confidentiality?
QuestionAnswer
Confidentiality-400
• ANSWER: These individuals should keep patient information private.
• QUESTION: Who is any medical personnel and those who have access to medical records?
QuestionAnswer
Confidentiality-500
• ANSWER: Violating patient confidentiality can possibly result in serious fines, criminal prosecution, and this.
• QUESTION: What is loss of employment?