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Telling The Truth Telling The Truth my experiences with Lyme borreliosis my experiences with Lyme borreliosis Bózsik, Béla Pál, M. Bózsik, Béla Pál, M. D. D. Lyme Borreliosis Foundation, Lyme Borreliosis Foundation, hUNGARY hUNGARY

Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

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Page 1: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Telling The TruthTelling The Truth– – my experiences with Lyme my experiences with Lyme

borreliosisborreliosis – –

Bózsik, Béla Pál, M. D.Bózsik, Béla Pál, M. D.Lyme Borreliosis Foundation,Lyme Borreliosis Foundation,

hUNGARY hUNGARY

Page 2: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

By Mel Gibson

think of the real truththink of the real truth

Dialogue on Dialogue on veritasveritas from Claudia & pilatusfrom Claudia & pilatus

What is the veritas, claudia?What is the veritas, claudia?Have You heard recognized if it would be said? Yes, I do!Have You heard recognized if it would be said? Yes, I do!How?-could you tell me?How?-could you tell me?If you have not heard it nobody could tell YouIf you have not heard it nobody could tell You

VERITAS?!–My verity is THAT any way it will bloodshed here!VERITAS?!–My verity is THAT any way it will bloodshed here!VERITAS?!–If it happens MY BLOOD WILL SHED BY KAISER!VERITAS?!–If it happens MY BLOOD WILL SHED BY KAISER!

If you have not heard the verityIf you have not heard the verity nobody could tell Younobody could tell You

The PassionThe PassionsequenceS ON THE TRUTHsequenceS ON THE TRUTH

Page 3: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

WELL,WELL, What is the TRUTH? What is the TRUTH?

OMAN:OMAN:

ANAN::

It could be personally It could be personally sensesense..

It depends on theIt depends on the principalitiesprincipalities..

It is better to sacrifice It is better to sacrifice oneonefor the people. John for the people. John 18,1418,14

ANHEDRIN:ANHEDRIN:

Page 4: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Y opinion:

What is the TRUTH What is the TRUTH in Lyme Borreliosis? in Lyme Borreliosis?

Lyme borreliosis

ENDEMIC both in the World & in Hungary is

Page 5: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Was estimated to affectWas estimated to affect

as MUCH as as MUCH as 10 %10 % of the populationof the population

at leastat leastBózsik, Lancet 2004Bózsik, Lancet 2004

Regarding to diagnostic and differential diagnostic difficulties there is no exact epidemiological data

Due to therapeutic problems and the long persistence of Lyme borreliosis the patients are accumulated in the population.

Bózsik, Vienna 1985Bózsik, Vienna 1985

This endemic This endemic Lyme borreliosisLyme borreliosis

Page 6: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

This Estimation based on bothThis Estimation based on both thethe possibility of tick’s co-infectionspossibility of tick’s co-infections& the cumulation of lyme borreliosis& the cumulation of lyme borreliosis

1: 101: 10

Lyme borreliosis incidence = ––––––––––– Lyme borreliosis incidence = ––––––––––– 200 TBE200 TBE_cases/years_cases/years

1: 1 000 1: 1 000

BacteriumBacterium

LB = TickLB = Tick’s infectivity’s infectivity rate with –––––––––––– rate with –––––––––––– TBE TBE_cases_cases

VirusVirus

Twenty thousends new cases yearly in Hungary

Page 7: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

What thisWhat thismeans & causes?means & causes?

No definiteNo definite Control popu- Control popu-

lationlationDiagnosis Diagnosis either ineither in laboratory laboratory oror clinical clinical Hampered Hampered by theby the protean symptomsprotean symptomsProceedings against Proceedings against MD-sMD-s

VainVain Fiery debates Fiery debates

in the society in the society . Heavy & long suffer-ing of Heavy & long suffer-ing of

patientspatients Decreased producti-vity of Decreased producti-vity of

the society.the society. Increased claims Increased claims

to rto rx . x . Increased Increased

medical costs medical costs . .

Page 8: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

ARE the FOLLOWINGARE the FOLLOWING The right answerS? The right answerS?

„„There is no such disease, There is no such disease,

if it could exist at all.”if it could exist at all.”

„„You surely not have this!”You surely not have this!”

Doctors SAY in all countries of the Doctors SAY in all countries of the world.world.

Could it really answeredCould it really answered

by serology? - I have to try it .by serology? - I have to try it .

Carl Brenner Science, 1992

Page 9: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

OUR PRACTICE WAS OUR PRACTICE WAS SINCE 1984 IN HUNGARYSINCE 1984 IN HUNGARY

0

4000

8000

12000

16000

20000

ESETSZÁM 139 715 2437 7071 11617 12283 13983 14207 13811 18698 14971

Vizsgálatszám 139 857 2689 7793 12752 13299 15003 15101 14596 19468 15863

POZITIV 106 420 1138 2958 3024 4549 3823 2369 3671 5013 3206

POZITIVITÁS % 76,3 49 42,3 38 23,7 34,2 25,5 15,7 25,2 25,8 20,2

1987-Vienna

1988-Vienna

89. 90. 91. 92. 93. 94. 95. 96. 97.

SEROLOGY FOR LYME BORRELIOSISJOHAN BÉLA NATIONAL INSTITUTE OF HYGIENE,

LABORATORY OF SEROLOGY 1987 - 1997109 932 CASES117 560 PHA-investigation 30 277 POSITIVE (25.8%) 1 349 ELISA-investigation 715 POSITIVE (53.0%)

Page 10: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Lyme borreliosis is endemic in Lyme borreliosis is endemic in hungary hungary

determinations at the nih, hungarydeterminations at the nih, hungary

Page 11: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Dinamic changing in serologyDinamic changing in serologyproduction of antibodiesproduction of antibodies

Summarized DiagramSummarized Diagram

Page 12: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Dinamics of Antibody production.Dinamics of Antibody production.It binds to every antigens It binds to every antigens of of borrelia burgdorferi s.l.borrelia burgdorferi s.l.

Effects of reinfection(s)Effects of reinfection(s)

Effects Effects onon IgMIgM production production

Persisting IgPersisting Igss & lb & lb

Eff

ects

of

ab

xEff

ects

of

ab

x

iigggg

Page 13: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Dinamic changing both in Dinamic changing both in serology & serology & production of antibodiesproduction of antibodies

Th

e p

rob

lem

is in

dete

rmin

ing

Th

e p

rob

lem

is in

dete

rmin

ing

th

e r

igh

t cu

t-off

level

the r

igh

t cu

t-off

level

infectioninfection

ecmecm

sec.sec.ecmecm

Changing in antigenecityCauses IgM elevations,

just as If it would be new infections

Decapitated, inhibited immune response

cut-off level?

Persistent IgPersisting lb

Spirochetaemia during the whole pathogenesis

TH

Page 14: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Is it possible to describe all of these Is it possible to describe all of these changes or changes or

diagnose Lyme borreliosis diagnose Lyme borreliosis

using any methodusing any method??

The question arised :The question arised :

No, that is the answer of course.No, that is the answer of course.

Determining the cut-off level Determining the cut-off level is also disputable is also disputable

as it is hard to determineas it is hard to determine

with with oneone investigation investigation

Page 15: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Lyme borreliosis reinfectiosaLyme borreliosis reinfectiosa

could only be revealed with early serologic testing, as

immune response of the body is slow & lasting: therefore existence of the antibody in the presence of ECM could be evaluated as reinfection.

can Lyme borreliosis be can Lyme borreliosis be over/UNDER-diagnosed over/UNDER-diagnosed

Mis-diagnosis Mis-diagnosis is the only possibility!is the only possibility!

Page 16: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Tail-cutting helps to evaluateTail-cutting helps to evaluate the results of passive haemagglutinationthe results of passive haemagglutination

The evaluation is based on 120,000 samples & 150,000 determinations since 1989.

The cut-off levelcan be calculated

from the resultsalone

Page 17: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

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Tail-cutting Tail-cutting for evaluating serologyfor evaluating serology

Reactivity of samples wereReactivity of samples wereDetermined by the dilution & cross(1-4)Determined by the dilution & cross(1-4)-Number versus 2/base log of dilution--Number versus 2/base log of dilution-

Highly reactive sera Highly reactive sera are few to delineateare few to delineate

Sera with low reactivitySera with low reactivity

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There are two populationsThere are two populationsSmall Positive(red)Small Positive(red)

Large Negative(blue)Large Negative(blue)They are Clearly dividedThey are Clearly divided

But missed But missed diagnosis of 30500 patientsdiagnosis of 30500 patients

Page 34: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

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Page 47: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

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Critical fields Critical fields to evaluationto evaluation

Page 48: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

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Critical fields Critical fields to evaluationto evaluation

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Critical fields Critical fields to evaluationto evaluation

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Critical fields Critical fields to evaluationto evaluation

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Critical fields Critical fields to evaluationto evaluation

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Critical fields Critical fields to evaluationto evaluation

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Critical fields Critical fields to evaluationto evaluation

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0

0,02

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5 5,5 6 6,5 7 7,5 8 8,5 9 9,5 10

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The parts of The lastsNormal distribution curves

for determining the cut-off level

Critical fields Critical fields to evaluationto evaluation

Page 57: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

7,6

7,8

8

8,2

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8,6

8,8

9

9,2

3132333435363738390

2

4

6

8

10

12

(log2Dilution) 2nd failure in % 1st failure in %

Plato areaWhere are potentially the less

missed cases

A Plato describedBy the errors of the curves

They Determined the cut-off levels

The cut-off level 1:200 dilution 3+ reactivity

Causing the smallest in both failures:Less the missed patients as seronegative: under 1,5% or it gives the less seropositivity to healthy:

under 5,5%missed 7,0%.

Although This is excellent result, it needs further evaluation For the benefit of the lyme-patients

10% missed

Page 58: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Which method could became Which method could became

the golden standard?the golden standard?

Or

Som

eO

r S

om

eFu

rth

er

new

meth

od

sFu

rth

er

new

meth

od

s

Page 59: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Lyme borreliosis seronegativaLyme borreliosis seronegativain relation to different methodsin relation to different methods

Page 60: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

As you have seenthere is a great difference between

biological & biostatistical error

Although 1.5 % first order and 5.5 % second order error are excellent results for any laboratory it is astonishing that the developments of science give possibility to determine further 60 % or more cases as people, who are in bed need of treatment.

This result is neither over-diagnosis, nor under-estimation, especially not fals/untrue determinations

they really belong to both the development of methodology and the epidemic character of Lyme borreliosis.

Page 61: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

I think of I think of many tenmany ten--thousands of peoplethousands of people

with suspected symptoms with suspected symptoms stated by their physicians stated by their physicians

Who could not get Who could not get the right answer or the right answer or

the definitive diagnosis.the definitive diagnosis.

Page 62: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

The BLUE COLUMNS The BLUE COLUMNS related to the missed proportions of related to the missed proportions of

patientspatients

theore

tical g

rap

h!

theore

tical g

rap

h!

Page 63: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

4/1802,2%

2/1801,1%negative

3/1801,7%

171/18095%Positive

negativePositive

Western-blot

There was not any reactivity in Master’s disease, in which B. burgd. sensu lato were isolated with substrain determination.

Masters,E–Philipp,M:Masters,E–Philipp,M: C6 Lyme peptide ELISA serosurvey of Missouri patients.Abstracts&Presentations at the IX Intl.Conf. on Lyme borreliosis &

Abstract at The Clinical side of Lyme Disease, N.Y. Augustus 18-22., 2002.

What is on C6-ELISA?What is on C6-ELISA?C6 ELISA & Wb

were identical in 97% at CDC comparing 180 samples

The molecular biologyThe molecular biology of the VlsEof the VlsE-antigen-antigen

is well known!is well known!

Page 64: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

It also teaches us It also teaches us that for evaluation that for evaluation

it should not be forgottenit should not be forgotten:: Lyme borreliosis Lyme borreliosis

Is a worldwide epidemicIs a worldwide epidemicWith countless ManifestationsWith countless Manifestations

The high incidence & The high incidence & especially extra high prevalenceespecially extra high prevalence

Is characteristic for Lyme borreliosisIs characteristic for Lyme borreliosis

Page 65: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

“The Committee recognizes that the current state of laboratory testing for Lyme disease is very poor. The situation has led many people to be misdiagnosed and delayed proper treatment. The vaccine clinical trial has documented that more that one third ( 36 percent ) of the people with Lyme disease did not test positive on the most sophisticated tests available. The ramifications of this deficit in terms of unnecessary pain, suffering and cost are staggering. The Committee directs CDC to work closely with the Food and Drug Administration to develop an unequivocal test for Lyme disease.( Public Law 107-116 Signed by President Bush 1/10/02 Departments of Labor, Health, and Human Services, and Education, and Related Agencies Appropriations Act 2002)

Page 66: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Is there Is there any solution any solution

of the diagnostic problemsof the diagnostic problems

Nearly one hundred yearsNearly one hundred yearsColeman, 1909Coleman, 1909

There has been a simple & good one!There has been a simple & good one!

It is possible It is possible To detect spirochetesTo detect spirochetes

With dark-field microscopyWith dark-field microscopy&&

since Nearly that time at leastsince Nearly that time at leastIt is debatedIt is debated

Because of the so-calledBecause of the so-calledMyelin figures, whichMyelin figures, whichLook like spirochetesLook like spirochetes

( pseudo-spirochetes )( pseudo-spirochetes )

Page 67: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

The largest energies The largest energies

in the naturein the nature

Among them Among them Is Is

the bloodthe blood

Page 68: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

There is direct connection There is direct connection between the body & the tickbetween the body & the tick

timetimeIs importantIs important

For the infectivity, becauseFor the infectivity, becauseTicks infect us Either with salivaTicks infect us Either with saliva

Or with regurgitation.Or with regurgitation.

this connection, this connection, makes possible for spirochetesmakes possible for spirochetes

to circulateto circulate&&

To reach the hidden places, too.To reach the hidden places, too.

Page 69: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

therefore, there is therefore, there is

no useno use to be distinguished to be distinguished

stagesstages for a generalized ailment,for a generalized ailment,

as it will be forgotten: as it will be forgotten:

Lyme borreliosis Lyme borreliosis damages the whole bodydamages the whole body

from the first moment.from the first moment.

Page 70: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

LLyme borreliosisyme borreliosis seronegativaseronegativa

one third ( 36 percent ) of the people with Lyme disease did not test positive

Public Law 107-116 Signed by President Bush 1/10/02

Nearly the same proportions Nearly the same proportions of lyme-patients of lyme-patients

were provedwere provedAs Lyme borreliosis seronegativa As Lyme borreliosis seronegativa in my practice with DF-microscopyin my practice with DF-microscopy

Page 71: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

My practice withMy practice withdarkfielddarkfield--microscopymicroscopy

was proven with realwas proven with real-time pcr-time pcr

Page 72: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

According to my practice According to my practice since 1986 since 1986

borrelia burgdorferi s. l. borrelia burgdorferi s. l. Could be demonstrated & studied with dark-Could be demonstrated & studied with dark-

field microscopy field microscopy during the whole pathogenesis.during the whole pathogenesis.

Besides clinical signs/symptoms Besides clinical signs/symptoms && serology serology it was proved it was proved

immunocytologically immunocytologically with kindly donated MCwith kindly donated MC

of Barbour & Steiner of CDC () of Barbour & Steiner of CDC () specific staining of rutenium red andspecific staining of rutenium red and

electron microscopy with negative staining.electron microscopy with negative staining.

Page 73: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

DualDurDualDur ®® reagent & methodreagent & method help to overcome difficulties in diagnosis of help to overcome difficulties in diagnosis of

Lyme borreliosis seronegativaLyme borreliosis seronegativa

first

second

Page 74: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Dividing Spirochete beside two

thrombocytes

Division of Spirochete happens

With Central concentration & splitting

Page 75: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

immuncytology (1990)immuncytology (1990): : Indirect immuno-fluorescence with Monoclonals anti-OspAIndirect immuno-fluorescence with Monoclonals anti-OspA és anti-és anti-flagellaris antitestekkel(prof.Barbour)flagellaris antitestekkel(prof.Barbour)

anti-anti-flflagellarisagellaris

Page 76: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

TheoreticalTheoretical – Length: 15-30 μm, Width: 0,15-0,30 μm, Flagellum: 7--20This oneThis one – Length ~15 μm, Width: 0,15-0,30 μm, NO Flagellum

Kari Hovind-Hougen, 1976-1986

Morphology of BorreliaeMorphology of Borreliae

Page 77: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

In 1909 & detailed in 1913:In 1909 & detailed in 1913:a new spirocheta was found a new spirocheta was found

in Human blood at graves’ diseasein Human blood at graves’ diseaseChambers,H-Royal Free Hospital

BMJ, 1909 & Lancet 1913,1:1728

„Diagrammatic representation of the Spirochetae seen in normal serumwhen examined by dark ground illumination”

(Some of these )

HOMOLOGIES between proteins of Borrelia burgdorferi & Thyroid AUTOANTIGENS

THYROID 2004, 14:86

great honourgreat honourto helen chambersto helen chambers

Page 78: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Borrelia burgdorferi s.s.Borrelia burgdorferi s.s. -Morphology in the friendly -Morphology in the friendly

cultivation media-cultivation media-Aberer-Duray, 1991

Meduza-like (paired-crossed-)

Double membranesVibrio-likeBipolar Taper

Treponema-like forms Paired Cells

Leptospira-likes Overlap/joinedElbow

UnipolarTaper

Before dividing? (Central splitting!)

End-joined Spirochetes

Page 79: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

various forms of various forms of Borrelia Borrelia burgd.s.l.burgd.s.l.

in blood usingin blood using DualDurDualDur®®After the basic study of Aberer-Duray, 1991

Double membranesVibrio-likeBipolar Taper

Treponema-like forms Paired Cells

Leptospira-likes Overlap/joinedElbow

UnipolarTaper

End-joined Spirochetes

Before dividing? (Central splitting!)

Page 80: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Patients’ Blood sample (27/72/105; 72/14/14) Phosphorwolframic-acid (1%) negativ staining, 1000x

Vesiculum, extrabacterialis proteinVesiculum, extrabacterialis protein bborrelia burgd.s.l.orrelia burgd.s.l.

Page 81: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

A. KERSTEN, C. POITSCHEK, S. RAUCH, AND E. ABERER: Effects of Penicillin,

Cettriaxone, and Doxycycline on Morphology of Borrelia burgdorferi

Antimicrob agent s chemother 1995,39:1127-33

Blebbing Blebbing in medical literaturein medical literatureDuring cultivation & under abxDuring cultivation & under abx

Ghost-likereorganizedmembrans

blebs

Page 82: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Extrabacterialis clouds in the layer of the Spirochete.Flagellum not visible.

Periplasmatic space

– Blood sample, Lyme-patient; Magnification 80.000x.

Page 83: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Borrelia burgdorferi s.l.Borrelia burgdorferi s.l. Immuncytologic experiments in 2003Immuncytologic experiments in 2003

Patient blood sample (GD-222)CDC, Monoclonalis antitest OspA+Avidin-Biotin Immunperoxidáz reaction,

Original magnification 100x

Control

Blood sample Blood sample

Blood

samples

Blood sample

Blood sample

Blood sample

Page 84: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Morphology ofMorphology of Borrelia burgdorferi Borrelia burgdorferi s.s.s.s. During cultivation - for controlDuring cultivation - for control

Page 85: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

Patient blood sample GD-223MC-OspA-AvidinBiotinPO

GD-223MC-OspA-AvidinBiotinPO

Shed ding for both defe nce & diseaseAs they are immu nocomplexes.

Page 86: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY
Page 87: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

There

were

spir

och

ete

s

There

were

spir

och

ete

s

in t

he p

ati

ents

’ blo

od s

am

ple

in t

he p

ati

ents

’ blo

od s

am

ple

Pati

en

tP

ati

en

t w

ith

Lym

e b

orr

eliosis

ch

ron

ica

wit

h L

ym

e b

orr

eliosis

ch

ron

ica

Was h

eale

dW

as h

eale

d

DarkfieldsDarkfieldsSame prep.Same prep.

stainedstained

Page 88: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

borreliae could be detected by specific borreliae could be detected by specific staining staining of of the outer layerthe outer layer with ruteniumwith rutenium--

red (dark-gray)red (dark-gray)

Please admire extra-bacterial materialPlease admire extra-bacterial materialaround the spirochetes (yellow)around the spirochetes (yellow)

Pati

en

tP

ati

en

t w

ith

Lym

e b

orr

eliosis

ch

ron

ica

wit

h L

ym

e b

orr

eliosis

ch

ron

ica

Was h

eale

dW

as h

eale

d

Acid Mucopolysaccharide

layer (dark gray)

protoplasmaprotoplasma

protoplasmaprotoplasma

Page 89: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

ClosingClosing remarksremarks

Definitive diagnosis Definitive diagnosis for Lyme borreliosis for Lyme borreliosis

could be set upcould be set up with laboratory methods.with laboratory methods.

indication indication of the treatmentof the treatmentneeds consultation,needs consultation,

that should be followedthat should be followedby control determinations by control determinations

for yearsfor years..

Page 90: Telling The Truth – my experiences with Lyme borreliosis – Bózsik, Béla Pál, M. D. Lyme Borreliosis Foundation, hUNGARY

we should have to unit we should have to unit our knowledgeour knowledge

„„As our enemy, the devil” As our enemy, the devil” is circulating &is circulating &

Wanted to devour us....Wanted to devour us....

11stst Letter of Peter 5 Letter of Peter 5ththchapter verse 8chapter verse 8