Porosity Response of Layer-by-Layer Nanocapsules on the Basis of Ultraviolet Light

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Porosity Response of Layer-by-Layer Nanocapsules on the Basis of Ultraviolet Light. David Huang University of Texas at Austin Department of Biomedical Engineering. Dr. Frank Ji, Ph.D. Drug Delivery via Nanocapsules. Nanocapsule shell of polymer nanoparticles - PowerPoint PPT Presentation

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  • Porosity Response of Layer-by-Layer Nanocapsules on the Basis of Ultraviolet LightDavid HuangUniversity of Texas at AustinDepartment of Biomedical EngineeringDr. Frank Ji, Ph.D.

  • Drug Delivery via NanocapsulesNanocapsule shell of polymer nanoparticles Held together by electrostatic forcesDrug kept inside until released

  • Benefits of NanocapsulesReceptors can be added without change to drug structureMinimize drug degradationIncrease drug bioavailability Dosage for drugs can be decreased by 10,000 folds

  • Controlling RateLooking for a method pulsatile releaseControlling Rate of Release of the Drug via light energyEnergized Bonds causes Conformational Change

  • Demonstration of ConceptWould conformational change really cause a noticeable difference in rate of release?Ultraviolet Light (355 nm) used especially just for demonstrationDrug replaced by Fluorescein dye to be detected once releasedPolymer used: Poly[1-[4-(3-carboxy-4-hydroxyphenylazo)benzenesulfonamido]-1,2-ethanediyl, sodium salt](PHES)

  • Plans to test ConceptCreate two samples of the nanocapsulesFill them with dyePlace them in two environmentsAbsence of lightUV emitterMeasure the rate of release of the dye

  • Creating and Filling NanocapsulesHFHFSilica ParticlePEIPHESFluorescein

  • Testing Phase

  • Chart1

    0000.818

    0000.828

    0000.8

    0000.878

    0000.906

    0000.969

    0001.027

    0001.102

    0001.227

    0001.371

    0001.51

    0001.714

    0001.946

    0002.195

    0002.468

    000.0782.645

    00.1250.2113.122

    00.2540.3683.751

    00.4370.5764.484

    0.0070.5360.7995.328

    0.0530.9321.116.193

    0.1611.3461.4337.324

    0.2541.8211.8358.49

    0.4022.1992.3259.818

    0.622.6272.95911.35

    0.8753.0963.6813.08

    1.1583.5964.51115.15

    1.4984.2865.47117.4

    1.9275.0186.61619.62

    2.4175.858.04822

    2.9866.8429.63824.74

    3.6087.98811.4127.61

    4.329.0513.530.57

    5.17410.315.6933.55

    5.9811.8118.0436.34

    6.94113.3520.6739.36

    8.04415.123.5342.18

    9.12616.7526.6745.15

    10.3618.4629.9347.76

    11.6720.2733.250.45

    12.921.9136.5652.56

    14.1423.5239.8654.81

    15.3325.4943.157.3

    16.6227.3146.1759.21

    17.6528.9248.5660.65

    18.6930.5651.2761.74

    19.6732.1253.6462.84

    20.3933.2555.2563.75

    20.8933.56757.0764.47

    21.434.26758.2364.66

    21.7534.67859.0664.73

    21.9435.12659.4464.64

    22.0535.6159.7764.63

    22.0535.23659.5564.26

    22.135.159.1963.98

    21.8334.12658.7263.3

    21.633.6257.7762.65

    21.233.29656.361.78

    20.7932.0555.1461.24

    20.2631.6553.5960.47

    19.6831.1951.9659.53

    19.1330.8350.6658.56

    18.4730.1949.1357.43

    17.9829.5647.3856.58

    17.3728.7745.5355.85

    16.6727.944.0754.98

    15.9826.9242.2554.1

    15.3326.0240.4953.08

    14.6725.0638.8852.35

    13.9924.0837.2751.58

    13.423.1935.7650.88

    12.7822.1434.3950.05

    12.2121.2533.1549.41

    11.7220.4131.948.74

    11.1219.6230.5548.2

    10.6418.7329.3847.69

    10.2317.9228.3147.34

    9.82317.1627.446.77

    9.44216.4726.3346.31

    9.06315.8125.4545.7

    8.7915.1224.6245.21

    8.5214.5223.8444.68

    8.19214.0223.0544

    7.90613.3722.3743.58

    7.62812.8821.7542.97

    7.35712.3821.0342.34

    7.19511.9820.4841.67

    7.03911.5519.9440.89

    6.81311.1419.2340.2

    6.67310.7618.6839.44

    6.42310.4718.0938.73

    6.1399.96217.5237.7

    5.869.55916.9536.83

    5.6359.35416.4335.92

    5.4549.08215.6735.11

    5.2428.75215.0534.12

    5.0438.46814.4833.07

    4.7758.19913.932.24

    4.5747.88413.3531.31

    4.317.54112.7830.3

    4.1417.17312.1629.25

    3.9446.89511.6128.11

    3.7426.61711.0327.09

    3.5246.35810.5326.29

    3.3256.069.96525.12

    3.1175.7399.36923.99

    2.8975.3978.78822.8

    2.7155.1778.31721.85

    2.5324.8937.8520.87

    2.3674.6257.37319.94

    2.2214.3186.9119.01

    2.054.0646.54918

    1.8623.8586.13417.12

    1.7343.5745.71516.32

    1.5993.2935.3215.58

    1.4763.0265.04614.86

    1.3522.8674.70814.03

    1.2212.6644.39613.33

    1.0792.4484.01912.63

    0.9772.2793.71611.95

    0.9042.0943.47611.3

    0.841.9133.24710.69

    0.7571.7173.04110.18

    0.7061.612.8279.662

    0.6181.4762.6049.247

    0.5441.3852.4348.864

    0.4941.2352.2848.4

    0.4221.1452.1178.044

    0.4011.0391.9977.604

    0.3480.961.8457.284

    0.3130.8661.6936.943

    0.2550.7661.5566.601

    0.1930.6961.466.326

    0.1630.6451.3776.104

    0.1520.6231.2955.8

    0.1060.5481.2285.48

    0.0760.481.1525.22

    0.0470.4141.0674.975

    0.0230.3810.9814.708

    0.0090.3080.8954.529

    0.0020.2750.8164.281

    Intensity after 5 min

    Intensity after 10 min

    Intensity after 15 min

    Intensity after 20 min

    Wavelength (nm)

    Fluorescent Intensity

    Fluorescent Intensity Released by Nanocapsules Energized by UV Light

    Chart2

    000.2360.8180.952

    000.2460.8281.24

    000.2510.81.235

    000.2440.8781.643

    000.2690.8951.782

    00.1340.3120.9691.709

    00.2450.3510.9951.923

    00.2780.3871.1022.352

    00.3460.461.2272.63

    00.480.5521.3712.873

    00.9340.6561.512.832

    01.1450.791.7142.965

    01.2580.9211.9462.987

    01.451.1232.1953.012

    01.3781.3742.4683.784

    01.4561.6632.8583.926

    01.6681.9753.3494.324

    01.7682.4353.8544.567

    01.8352.8854.4745.432

    0.0712.5463.355.1785.632

    0.2132.7683.9956.0826.125

    0.3562.8754.7537.0497.432

    0.522.9875.6268.2028.346

    0.7453.0116.4828.5549.231

    1.014.5637.6189.8489.658

    1.3594.6788.7411.313.235

    1.8024.8799.94613.0415.57

    2.3285.32611.4814.8515.687

    2.9386.43213.1516.9318.325

    3.6167.45614.941919.543

    4.5129.45316.620.8921.1

    5.45710.23418.4321.421.25

    6.53112.47520.3522.5423.54

    7.69714.57222.2623.4325.71

    8.93715.567224.125.4327.87

    10.3517.28325.9926.32530.17

    11.9218.567827.8629.4532.41

    13.5820.23529.7931.2334.6

    15.3122.47331.6833.25636.92

    17.224.83833.2335.1238.85

    18.9426.478334.5935.67840.51

    20.7628.5436.137.12542.2

    22.3632.56737.2639.2543.55

    24.2633.567238.5440.2344.79

    25.834.562739.5941.68545.82

    27.2835.67840.1542.3846.76

    28.4436.12540.7542.987447.51

    29.5136.83741.2643.78548.04

    30.2537.1241.5943.97548.53

    31.0437.98741.8844.1548.69

    31.4338.01242.0244.3248.91

    31.9138.3742.0144.4348.63

    32.138.12541.944.6148.21

    32.0537.987741.7544.2348.02

    31.6537.45241.2843.76547.72

    31.1936.89540.9142.96247.44

    30.8335.5640.6142.56346.66

    30.1934.675340.0542.1246.07

    29.5633.98739.4241.65745.38

    28.7732.85438.7440.49844.66

    27.932.36438.1640.21543.95

    26.9231.59637.6939.25843.19

    26.0231.27437.0739.142.53

    25.0630.48636.4238.4341.68

    24.0829.9835.8337.8441.1

    23.1929.12535.2437.2140.53

    22.1428.45834.5436.5439.96

    21.2528.12534.0436.239.1

    20.4127.68533.3335.4238.52

    19.6227.12533.0735.2138.2

    18.7326.98432.634.7537.62

    17.9226.34132.2934.5636.97

    17.1625.87431.8533.4536.43

    16.4724.9331.2733.2536.04

    15.8124.5130.8432.8435.69

    15.1224.0130.5232.5135.52

    14.5223.630.1532.1535.02

    14.0223.1629.8431.8434.55

    13.3722.7329.5731.5434.3

    12.8822.2329.2431.2533.96

    12.3821.8728.9730.9533.39

    11.9821.4128.530.5432.97

    11.5520.8128.1430.1232.6

    11.1420.1827.5829.56732.08

    10.7620.4127.2229.2231.74

    10.4719.6226.7928.7531.17

    9.96218.6226.6328.6330.43

    9.55917.8226.0728.0130.05

    9.35417.0925.4627.4629.57

    9.08216.4824.9726.9728.9

    8.75215.8424.3726.4728.2

    8.46815.1823.825.76527.67

    8.19914.5223.2524.78626.99

    7.88413.922.824.59826.38

    7.54113.3122.2324.37225.85

    7.17312.5621.6223.15625.12

    6.89511.8821.0722.67824.18

    6.61711.2920.5522.57323.4

    6.35810.6519.7622.12422.72

    6.0610.2319.2321.54322.02

    5.7399.82318.6221.34621.2

    5.3979.44217.8220.78420.55

    5.1779.06317.0919.56219.78

    4.8938.7916.4819.25618.9

    4.6258.5215.8418.54618.28

    4.3188.19215.1817.25917.43

    4.0647.90614.5216.12616.63

    3.8587.62813.915.32515.85

    3.5747.35713.3114.87515.19

    3.2937.19512.5614.2115.01

    3.0267.03911.8813.25814.285

    2.8676.81311.2912.45213.483

    2.6646.67310.6511.25312.235

    2.4486.42310.110.87511.857

    2.2796.1399.60210.25411.251

    2.0945.869.17310.12910.98

    1.9135.6358.6619.87410.583

    1.7175.4548.2789.25110.125

    1.615.2427.8438.9859.93

    1.4765.0437.4158.1259.231

    1.3854.7757.0427.9238.893

    1.2354.5746.6567.258.251

    1.1454.316.3026.8437.832

    1.0394.1416.0356.5827.351

    0.963.9445.7646.5216.982

    0.8663.7425.4516.2016.238

    0.7663.5245.1165.9836.018

    0.6963.3254.8095.6215.716

    0.6453.1174.5935.215.448

    0.6232.8974.4764.8295.146

    0.5482.7154.2654.6724.947

    0.482.5324.0274.3254.733

    0.4142.3673.8284.2154.429

    0.3812.2213.6214.1854.239

    0.3082.053.4783.8274.039

    0.2751.8623.3363.7213.886

    0.2511.7343.1423.6123.695

    0.2081.5992.9943.3153.467

    0.1711.4762.8473.2713.312

    0.1361.3522.7083.1853.148

    0.0921.2212.5342.853.01

    Intensity after 5 min

    Intensity after 10 min

    Intensity after 15 min

    Intensity after 20 min

    Intensity after 25 min

    Wavelength (nm)

    Fluorescent Intensity

    Fluorescent Intensity Released by Nanocapsules in the Absence of Light

    Chart3

    32.122.1

    38.3735.61

    42.0259.77

    44.6164.73

    48.9164.23

    45.3263.32

    39.1458.72

    26.8755.28

    26.2147.68

    26.0827.63

    Nanocapsules under the absence of light

    Nanocapsules energized by UV light

    Time (min)

    Fluorescent Intensity

    Fluorescent Intensity Released by Nanocapsules over Time

    Chart4

    32.122.1

    38.3735.61

    42.0259.77

    44.6164.73

    48.9120

    In the Absence of Light

    Energized by UV Light

    Time (min)

    Fluorescent Intensity

    Increasing Fluorescent Intensity Released by Nanocapsules over Time

    Sheet1

    DK1UV1TimePeaks

    Data PointsData PointsDKUVDKUV

    nmDataDataDataDataDataDataDatanmDataDataDataDataDataData032.122.122.9824.12

    460000.2360.8180.9520.8530.3715.4575.1644.6854600000.8180.8010.7940.3610.3510.3710.236538.3735.6126.6929.31

    461000.2460.8281.240.9340.3995.3595.1614.4894610000.8280.8670.8120.3790.3670.3990.2461042.0259.7727.729.6

    462000.2510.81.2350.9520.3554.7494.7284.0284620000.80.8430.8250.3930.3870.3550.2511544.6164.7329.632.19

    463000.2440.8781.6431.1560.3564.0224.0453.4224630000.8780.8310.8340.4260.4150.3560.2442048.9164.2330.447.75

    464000.2690.8951.7821.2560.3553.4083.3822.8714640000.9060.8920.8660.4820.4260.3550.2692545.3263.3234.640.32

    46500.1340.3120.9691.7091.3560.3532.8632.8432.3854650000.9690.9350.9120.570.4860.3530.3123039.1458.7227.833.21

    46600.2450.3510.9951.9231.5720.3762.4882.4582.074660001.0270.9750.9430.6690.5830.3760.3516026.8755.2823.6227.67

    46700.2780.3871.1022.3521.6920.4282.3222.2241.9224670001.1021.0480.9650.7580.7230.4280.38712026.2147.6820.4524.68

    46800.3460.461.2272.631.9280.4972.2242.1151.8714680001.2271.1531.2560.8540.7930.4970.4624026.0827.6319.2319.16

    46900.480.5521.3712.8732.0510.5522.1692.0791.8944690001.3711.2251.3460.9860.8950.5520.552

    47000.9340.6561.512.8322.1560.6182.1081.9951.8444700001.511.361.8671.1591.0450.6180.656

    47101.1450.791.7142.9652.2560.7412.0511.9731.7634710001.7141.511.6731.3521.2450.7410.79

    47201.2580.9211.9462.9872.4160.8652.061.9281.7994720001.9461.7541.6921.5681.3560.8650.921

    47301.451.1232.1953.0122.7121.0432.1281.9781.8734730002.1951.9761.8761.8441.5861.0431.123

    47401.3781.3742.4683.7842.8361.2432.1532.0131.9574740002.4682.2472.1252.2391.6791.2431.374032.122.1

    47501.4561.6632.8583.9263.2361.5342.1972.0552.012475000.0782.6452.6332.5462.8582.5981.5341.663538.3735.61

    47601.6681.9753.3494.3243.5671.8212.2812.1222.12347600.1250.2113.1223.2363.1253.3493.0631.8211.9751042.0259.77

    47701.7682.4353.8544.5673.6322.1992.4762.3162.2947700.2540.3683.7513.5673.2353.8543.5612.1992.4351544.6164.73

    47801.8352.8854.4745.4323.7352.6272.6762.5222.50947800.4370.5764.4844.3264.1294.4744.0962.6272.8852048.91

    4790.0712.5463.355.1785.6324.2163.0962.9322.7392.7754790.0070.5360.7995.3285.2314.685.1784.7343.0963.5625

    4800.2132.7683.9956.0826.1254.4563.5963.2943.1043.1974800.0530.9321.116.1936.2375.436.0825.4693.5963.96

    4810.3562.8754.7537.0497.4325.2364.2863.7643.5273.6584810.1611.3461.4337.3247.2126.977.0496.4194.2864.097

    4820.522.9875.6268.2028.3465.6325.0184.2563.9844.174820.2541.8211.8358.498.3627.348.2027.4375.0184.526

    4830.7453.0116.4828.5549.2316.3255.854.7694.5414.7164830.4022.1992.3259.8189.7238.459.4458.5545.855.141

    4841.014.5637.6189.8489.6586.8926.8425.3765.1525.3934840.622.6272.95911.3510.3269.23410.839.8486.8425.728

    4851.3594.6788.7411.313.2358.3467.9886.1025.8166.1224850.8753.0963.6813.0811.23510.2312.3911.37.7626.394

    4861.8024.8799.94613.0415.579.2369.056.8126.5346.9054861.1583.5964.51115.1515.23614.2314.1813.048.6687.16

    4872.3285.32611.4814.8515.68710.23610.37.6057.3597.7624871.4984.2865.47117.416.23615.23616.2214.8511.428.014

    4882.9386.43213.1516.9318.32512.36211.818.4748.3118.6684881.9275.0186.61619.6218.23616.2318.2816.9313.258.936

    4893.6167.45614.941919.54315.23613.359.4429.2199.6284892.4175.858.0482221.2520.4320.531915.949.855

    4904.5129.45316.620.8921.115.89215.110.5110.2810.734902.9866.8429.63824.7424.53623.6522.8521.0818.35210.99

    4915.45710.23418.4321.421.2518.32616.7511.5911.3611.914913.6087.98811.4127.6126.23525.3425.4823.2519.5412.18

    4926.53112.47520.3522.5423.5421.23618.4612.8112.5913.124924.329.0513.530.5729.12628.5628.0225.8121.5313.35

    4937.69714.57222.2623.4325.7121.32620.2713.9813.7714.244935.17410.315.6933.5531.23630.6530.728.3524.3514.6

    4948.93715.567224.125.4327.8722.56121.9115.1314.8915.414945.9811.8118.0436.3432.36231.2533.3230.9128.43515.71

    49510.3517.28325.9926.32530.1724.26123.5216.2515.9916.644956.94113.3520.6739.3635.235.6435.7733.2329.3216.95

    49611.9218.567827.8629.4532.4125.98625.4917.4917.1517.764968.04415.123.5342.1839.12538.4538.4535.7731.2518.14

    49713.5820.23529.7931.2334.627.89327.3118.5718.3319.064979.12616.7526.6745.1542.12541.2341.0338.2534.2519.24

    49815.3122.47331.6833.25636.9230.2628.9219.619.4420.0749810.3618.4629.9347.7645.23642.6543.5340.5135.4220.35

    49917.224.83833.2335.1238.8535.21630.5620.5720.4421.0849911.6720.2733.250.4549.23648.2345.7542.7236.3321.4

    50018.9426.478334.5935.67840.5138.26132.1221.4321.2721.9850012.921.9136.5652.5651.2651.6747.9344.6437.122.33

    50120.7628.5436.137.12542.240.63733.2522.3222.0422.9950114.1423.5239.8654.8153.23652.6849.846.5737.8423.27

    50222.3632.56737.2639.2543.5540.98234.2923.2322.8323.7350215.3325.4943.157.358.43655.3251.7348.1838.3224.29

    50324.2633.567238.5440.2344.7941.26935.4223.9723.6524.4650316.6227.3146.1759.2159.23656.5453.2349.8638.6525.02

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    50527.2835.67840.1542.3846.7642.69437.125.1724.7125.6150518.6930.5651.2761.7461.32659.2355.8852.1742.5626.26

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    Sheet2

    Sheet3

  • Fluorescent Intensity Released by Nanocapsules Energized by UV Light

    Chart1

    0000.818

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    Intensity after 5 min

    Intensity after 10 min

    Intensity after 15 min

    Intensity after 20 min

    Wavelength (nm)

    Fluorescent Intensity

    Chart2

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    000.2440.8781.643

    000.2690.8951.782

    00.1340.3120.9691.709

    00.2450.3510.9951.923

    00.2780.3871.1022.352

    00.3460.461.2272.63

    00.480.5521.3712.873

    00.9340.6561.512.832

    01.1450.791.7142.965

    01.2580.9211.9462.987

    01.451.1232.1953.012

    01.3781.3742.4683.784

    01.4561.6632.8583.926

    01.6681.9753.3494.324

    01.7682.4353.8544.567

    01.8352.8854.4745.432

    0.0712.5463.355.1785.632

    0.2132.7683.9956.0826.125

    0.3562.8754.7537.0497.432

    0.522.9875.6268.2028.346

    0.7453.0116.4828.5549.231

    1.014.5637.6189.8489.658

    1.3594.6788.7411.313.235

    1.8024.8799.94613.0415.57

    2.3285.32611.4814.8515.687

    2.9386.43213.1516.9318.325

    3.6167.45614.941919.543

    4.5129.45316.620.8921.1

    5.45710.23418.4321.421.25

    6.53112.47520.3522.5423.54

    7.69714.57222.2623.4325.71

    8.93715.567224.125.4327.87

    10.3517.28325.9926.32530.17

    11.9218.567827.8629.4532.41

    13.5820.23529.7931.2334.6

    15.3122.47331.6833.25636.92

    17.224.83833.2335.1238.85

    18.9426.478334.5935.67840.51

    20.7628.5436.137.12542.2

    22.3632.56737.2639.2543.55

    24.2633.567238.5440.2344.79

    25.834.562739.5941.68545.82

    27.2835.67840.1542.3846.76

    28.4436.12540.7542.987447.51

    29.5136.83741.2643.78548.04

    30.2537.1241.5943.97548.53

    31.0437.98741.8844.1548.69

    31.4338.01242.0244.3248.91

    31.9138.3742.0144.4348.63

    32.138.12541.944.6148.21

    32.0537.987741.7544.2348.02

    31.6537.45241.2843.76547.72

    31.1936.89540.9142.96247.44

    30.8335.5640.6142.56346.66

    30.1934.675340.0542.1246.07

    29.5633.98739.4241.65745.38

    28.7732.85438.7440.49844.66

    27.932.36438.1640.21543.95

    26.9231.59637.6939.25843.19

    26.0231.27437.0739.142.53

    25.0630.48636.4238.4341.68

    24.0829.9835.8337.8441.1

    23.1929.12535.2437.2140.53

    22.1428.45834.5436.5439.96

    21.2528.12534.0436.239.1

    20.4127.68533.3335.4238.52

    19.6227.12533.0735.2138.2

    18.7326.98432.634.7537.62

    17.9226.34132.2934.5636.97

    17.1625.87431.8533.4536.43

    16.4724.9331.2733.2536.04

    15.8124.5130.8432.8435.69

    15.1224.0130.5232.5135.52

    14.5223.630.1532.1535.02

    14.0223.1629.8431.8434.55

    13.3722.7329.5731.5434.3

    12.8822.2329.2431.2533.96

    12.3821.8728.9730.9533.39

    11.9821.4128.530.5432.97

    11.5520.8128.1430.1232.6

    11.1420.1827.5829.56732.08

    10.7620.4127.2229.2231.74

    10.4719.6226.7928.7531.17

    9.96218.6226.6328.6330.43

    9.55917.8226.0728.0130.05

    9.35417.0925.4627.4629.57

    9.08216.4824.9726.9728.9

    8.75215.8424.3726.4728.2

    8.46815.1823.825.76527.67

    8.19914.5223.2524.78626.99

    7.88413.922.824.59826.38

    7.54113.3122.2324.37225.85

    7.17312.5621.6223.15625.12

    6.89511.8821.0722.67824.18

    6.61711.2920.5522.57323.4

    6.35810.6519.7622.12422.72

    6.0610.2319.2321.54322.02

    5.7399.82318.6221.34621.2

    5.3979.44217.8220.78420.55

    5.1779.06317.0919.56219.78

    4.8938.7916.4819.25618.9

    4.6258.5215.8418.54618.28

    4.3188.19215.1817.25917.43

    4.0647.90614.5216.12616.63

    3.8587.62813.915.32515.85

    3.5747.35713.3114.87515.19

    3.2937.19512.5614.2115.01

    3.0267.03911.8813.25814.285

    2.8676.81311.2912.45213.483

    2.6646.67310.6511.25312.235

    2.4486.42310.110.87511.857

    2.2796.1399.60210.25411.251

    2.0945.869.17310.12910.98

    1.9135.6358.6619.87410.583

    1.7175.4548.2789.25110.125

    1.615.2427.8438.9859.93

    1.4765.0437.4158.1259.231

    1.3854.7757.0427.9238.893

    1.2354.5746.6567.258.251

    1.1454.316.3026.8437.832

    1.0394.1416.0356.5827.351

    0.963.9445.7646.5216.982

    0.8663.7425.4516.2016.238

    0.7663.5245.1165.9836.018

    0.6963.3254.8095.6215.716

    0.6453.1174.5935.215.448

    0.6232.8974.4764.8295.146

    0.5482.7154.2654.6724.947

    0.482.5324.0274.3254.733

    0.4142.3673.8284.2154.429

    0.3812.2213.6214.1854.239

    0.3082.053.4783.8274.039

    0.2751.8623.3363.7213.886

    0.2511.7343.1423.6123.695

    0.2081.5992.9943.3153.467

    0.1711.4762.8473.2713.312

    0.1361.3522.7083.1853.148

    0.0921.2212.5342.853.01

    Intensity after 5 min

    Intensity after 10 min

    Intensity after 15 min

    Intensity after 20 min

    Intensity after 25 min

    Wavelength (nm)

    Fluorescent Intensity

    Fluorescent Intensity Released by Nanocapsules in the Absence of Light

    Chart3

    32.122.1

    38.3735.61

    42.0259.77

    44.6164.73

    48.9164.23

    45.3263.32

    39.1458.72

    26.8755.28

    26.2147.68

    26.0827.63

    Nanocapsules under the absence of light

    Nanocapsules energized by UV light

    Time (min)

    Fluorescent Intensity

    Fluorescent Intensity Released by Nanocapsules over Time

    Chart4

    32.122.1

    38.3735.61

    42.0259.77

    44.6164.73

    48.9120

    In the Absence of Light

    Energized by UV Light

    Time (min)

    Fluorescent Intensity

    Increasing Fluorescent Intensity Released by Nanocapsules over Time

    Sheet1

    DK1UV1TimePeaks

    Data PointsData PointsDKUVDKUV

    nmDataDataDataDataDataDataDatanmDataDataDataDataDataData032.122.122.9824.12

    460000.2360.8180.9520.8530.3715.4575.1644.6854600000.8180.8010.7940.3610.3510.3710.236538.3735.6126.6929.31

    461000.2460.8281.240.9340.3995.3595.1614.4894610000.8280.8670.8120.3790.3670.3990.2461042.0259.7727.729.6

    462000.2510.81.2350.9520.3554.7494.7284.0284620000.80.8430.8250.3930.3870.3550.2511544.6164.7329.632.19

    463000.2440.8781.6431.1560.3564.0224.0453.4224630000.8780.8310.8340.4260.4150.3560.2442048.9164.2330.447.75

    464000.2690.8951.7821.2560.3553.4083.3822.8714640000.9060.8920.8660.4820.4260.3550.2692545.3263.3234.640.32

    46500.1340.3120.9691.7091.3560.3532.8632.8432.3854650000.9690.9350.9120.570.4860.3530.3123039.1458.7227.833.21

    46600.2450.3510.9951.9231.5720.3762.4882.4582.074660001.0270.9750.9430.6690.5830.3760.3516026.8755.2823.6227.67

    46700.2780.3871.1022.3521.6920.4282.3222.2241.9224670001.1021.0480.9650.7580.7230.4280.38712026.2147.6820.4524.68

    46800.3460.461.2272.631.9280.4972.2242.1151.8714680001.2271.1531.2560.8540.7930.4970.4624026.0827.6319.2319.16

    46900.480.5521.3712.8732.0510.5522.1692.0791.8944690001.3711.2251.3460.9860.8950.5520.552

    47000.9340.6561.512.8322.1560.6182.1081.9951.8444700001.511.361.8671.1591.0450.6180.656

    47101.1450.791.7142.9652.2560.7412.0511.9731.7634710001.7141.511.6731.3521.2450.7410.79

    47201.2580.9211.9462.9872.4160.8652.061.9281.7994720001.9461.7541.6921.5681.3560.8650.921

    47301.451.1232.1953.0122.7121.0432.1281.9781.8734730002.1951.9761.8761.8441.5861.0431.123

    47401.3781.3742.4683.7842.8361.2432.1532.0131.9574740002.4682.2472.1252.2391.6791.2431.374032.122.1

    47501.4561.6632.8583.9263.2361.5342.1972.0552.012475000.0782.6452.6332.5462.8582.5981.5341.663538.3735.61

    47601.6681.9753.3494.3243.5671.8212.2812.1222.12347600.1250.2113.1223.2363.1253.3493.0631.8211.9751042.0259.77

    47701.7682.4353.8544.5673.6322.1992.4762.3162.2947700.2540.3683.7513.5673.2353.8543.5612.1992.4351544.6164.73

    47801.8352.8854.4745.4323.7352.6272.6762.5222.50947800.4370.5764.4844.3264.1294.4744.0962.6272.8852048.91

    4790.0712.5463.355.1785.6324.2163.0962.9322.7392.7754790.0070.5360.7995.3285.2314.685.1784.7343.0963.5625

    4800.2132.7683.9956.0826.1254.4563.5963.2943.1043.1974800.0530.9321.116.1936.2375.436.0825.4693.5963.96

    4810.3562.8754.7537.0497.4325.2364.2863.7643.5273.6584810.1611.3461.4337.3247.2126.977.0496.4194.2864.097

    4820.522.9875.6268.2028.3465.6325.0184.2563.9844.174820.2541.8211.8358.498.3627.348.2027.4375.0184.526

    4830.7453.0116.4828.5549.2316.3255.854.7694.5414.7164830.4022.1992.3259.8189.7238.459.4458.5545.855.141

    4841.014.5637.6189.8489.6586.8926.8425.3765.1525.3934840.622.6272.95911.3510.3269.23410.839.8486.8425.728

    4851.3594.6788.7411.313.2358.3467.9886.1025.8166.1224850.8753.0963.6813.0811.23510.2312.3911.37.7626.394

    4861.8024.8799.94613.0415.579.2369.056.8126.5346.9054861.1583.5964.51115.1515.23614.2314.1813.048.6687.16

    4872.3285.32611.4814.8515.68710.23610.37.6057.3597.7624871.4984.2865.47117.416.23615.23616.2214.8511.428.014

    4882.9386.43213.1516.9318.32512.36211.818.4748.3118.6684881.9275.0186.61619.6218.23616.2318.2816.9313.258.936

    4893.6167.45614.941919.54315.23613.359.4429.2199.6284892.4175.858.0482221.2520.4320.531915.949.855

    4904.5129.45316.620.8921.115.89215.110.5110.2810.734902.9866.8429.63824.7424.53623.6522.8521.0818.35210.99

    4915.45710.23418.4321.421.2518.32616.7511.5911.3611.914913.6087.98811.4127.6126.23525.3425.4823.2519.5412.18

    4926.53112.47520.3522.5423.5421.23618.4612.8112.5913.124924.329.0513.530.5729.12628.5628.0225.8121.5313.35

    4937.69714.57222.2623.4325.7121.32620.2713.9813.7714.244935.17410.315.6933.5531.23630.6530.728.3524.3514.6

    4948.93715.567224.125.4327.8722.56121.9115.1314.8915.414945.9811.8118.0436.3432.36231.2533.3230.9128.43515.71

    49510.3517.28325.9926.32530.1724.26123.5216.2515.9916.644956.94113.3520.6739.3635.235.6435.7733.2329.3216.95

    49611.9218.567827.8629.4532.4125.98625.4917.4917.1517.764968.04415.123.5342.1839.12538.4538.4535.7731.2518.14

    49713.5820.23529.7931.2334.627.89327.3118.5718.3319.064979.12616.7526.6745.1542.12541.2341.0338.2534.2519.24

    49815.3122.47331.6833.25636.9230.2628.9219.619.4420.0749810.3618.4629.9347.7645.23642.6543.5340.5135.4220.35

    49917.224.83833.2335.1238.8535.21630.5620.5720.4421.0849911.6720.2733.250.4549.23648.2345.7542.7236.3321.4

    50018.9426.478334.5935.67840.5138.26132.1221.4321.2721.9850012.921.9136.5652.5651.2651.6747.9344.6437.122.33

    50120.7628.5436.137.12542.240.63733.2522.3222.0422.9950114.1423.5239.8654.8153.23652.6849.846.5737.8423.27

    50222.3632.56737.2639.2543.5540.98234.2923.2322.8323.7350215.3325.4943.157.358.43655.3251.7348.1838.3224.29

    50324.2633.567238.5440.2344.7941.26935.4223.9723.6524.4650316.6227.3146.1759.2159.23656.5453.2349.8638.6525.02

    50425.834.562739.5941.68545.8241.64836.3324.5724.1925.1350417.6528.9248.5660.6560.76558.7854.651.0741.9225.72

    50527.2835.67840.1542.3846.7642.69437.125.1724.7125.6150518.6930.5651.2761.7461.32659.2355.8852.1742.5626.26

    50628.4436.12540.7542.987447.5143.99737.8425.4525.0926.1650619.6732.1253.6462.8462.36560.3456.7753.0143.6726.66

    50729.5136.83741.2643.78548.0444.02538.3225.6425.426.450720.3933.2555.2563.7563.67861.2457.3553.7844.5327.06

    50830.2537.1241.5943.97548.5344.72138.6525.9525.8426.7250820.8933.56757.0764.4763.98661.4957.8754.3846.4327.25

    50931.0437.98741.8844.1548.6945.02139.0426.1426.0826.8250921.434.26758.2364.6664.12562.4558.3354.7846.9827.43

    51031.4338.01242.0244.3248.9145.3239.1426.8726.2126.8451021.7534.67859.0664.7364.21962.7858.4455.1847.2627.49

    51131.9138.3742.0144.4348.6345.20639.1126.0326.126.0851121.9435.12659.4464.6464.2363.3258.6755.2847.6827.63

    51232.138.12541.944.6148.2144.21638.9325.9925.9926.7651222.0535.6159.7764.6363.26162.3458.5655.1746.9427.66

    51332.0537.987741.7544.2348.0243.65738.6126.0225.8826.751322.0535.23659.5564.2663.12962.1958.2654.845.6827.42

    51431.6537.45241.2843.76547.7243.19538.2525.9125.7326.4851422.135.159.1963.9862.31261.2357.6354.5445.2327.18

    51531.1936.89540.9142.96247.4442.17537.9125.5525.5226.2951521.8334.12658.7263.360.23160.1657.2553.8744.7527.03

    51630.8335.5640.6142.56346.6641.92437.6825.3125.2625.9551621.633.6257.7762.6561.23959.5656.5753.243.2626.95

    51730.1934.675340.0542.1246.0741.82737.3124.9224.9425.6151721.233.29656.361.7860.98259.2355.9252.6842.6926.61

    51829.5633.98739.4241.65745.3841.79536.5824.7124.7525.2851820.7932.0555.1461.2460.84358.9655.2152.0941.2826.28

    51928.7732.85438.7440.49844.6640.87536.1324.3624.5125.0451920.2631.6553.5960.4759.23658.7554.4951.4741.125.9

    52027.932.36438.1640.21543.9540.67235.4823.8223.9424.6152019.6831.1951.9659.5359.126658.2353.6750.6940.4625.53

    52126.9231.59637.6939.25843.1940.51834.8323.3623.5824.4152119.1330.8350.6658.5658.32857.3452.9949.9139.5125.1

    52226.0231.27437.0739.142.5339.7434.3123.0423.3123.8552218.4730.1949.1357.4357.65456.4652.0949.2638.3524.64

    52325.0630.48636.4238.4341.6839.47133.8422.6722.8723.4452317.9829.5647.3856.5856.23856.2451.1948.4837.2624.18

    52424.0829.9835.8337.8441.137.57233.322.422.623.1452417.3728.7745.5355.8555.12655.6750.4447.5736.5823.66

    52523.1929.12535.2437.2140.5337.19232.7722.0222.2322.6952516.6727.944.0754.9853.68254.2349.8746.935.4123.26

    52622.1428.45834.5436.5439.9636.38332.221.6721.922.4152615.9826.9242.2554.154.86753.1649.1146.1634.3822.99

    52721.2528.12534.0436.239.135.47231.7521.3421.5622.1452715.3326.0240.4953.0852.12852.3548.3945.5833.2722.65

    52820.4127.68533.3335.4238.5234.32831.2421.0621.3721.8452814.6725.0638.8852.3551.23651.9647.6344.8632.9622.4

    52919.6227.12533.0735.2138.234.12830.7920.8821.0621.4852913.9924.0837.2751.5850.23649.2646.7644.1932.7522.17

    53018.7326.98432.634.7537.6233.82530.3620.5820.7321.1753013.423.1935.7650.8849.23648.2345.9843.5631.5721.89

    53117.9226.34132.2934.5636.9733.72630.0220.3620.4121.0453112.7822.1434.3950.0549.3248.1545.5242.9830.2721.51

    53217.1625.87431.8533.4536.4333.46129.6620.0320.1820.8853212.2121.2533.1549.4148.9647.2344.8442.3730.4621.23

    53316.4724.9331.2733.2536.0432.15629.1819.7719.9220.5753311.7220.4131.948.7448.1247.7844.4241.9329.5721.14

    53415.8124.5130.8432.8435.6932.01228.7519.4819.7720.2853411.1219.6230.5548.247.1346.7843.9441.4129.3420.81

    53515.1224.0130.5232.5135.5231.87628.4719.1919.5720.1353510.6418.7329.3847.6946.8946.2443.5340.9428.4520.55

    53614.5223.630.1532.1535.0231.73228.1419.1119.2319.8453610.2317.9228.3147.3446.5745.6842.8840.628.1420.29

    53714.0223.1629.8431.8434.5531.57227.6818.8518.9519.45379.82317.1627.446.7745.2145.3642.4940.227.5620.15

    53813.3722.7329.5731.5434.330.57127.418.7518.7619.245389.44216.4726.3346.3145.1244.1241.8139.7527.4319.85

    53912.8822.2329.2431.2533.9630.25127.0618.518.6619.155399.06315.8125.4545.744.6743.2641.3339.1426.5819.69

    54012.3821.8728.9730.9533.3929.78526.6718.1918.3718.925408.7915.1224.6245.2144.2343.1640.9838.6626.3619.46

    54111.9821.4128.530.5432.9728.5626.3817.9318.2418.685418.5214.5223.8444.6843.1242.6740.5138.2425.3819.21

    54211.5520.8128.1430.1232.628.45626.0817.7817.8718.475428.19214.0223.054442.6742.3639.9237.7525.118.94

    54311.1420.1827.5829.56732.0827.34525.7417.5917.6218.235437.90613.3722.3743.5842.1241.6739.3237.2624.7318.6

    54410.7620.4127.2229.2231.7427.12925.3317.3417.5117.935447.62812.8821.7542.9741.8641.2938.9436.6324.1618.38

    54510.4719.6226.7928.7531.1726.89524.9317.0817.2917.75457.35712.3821.0342.3441.5640.2638.2936.0623.4718.19

    5469.96218.6226.6328.6330.4326.78424.5116.781717.45467.19511.9820.4841.6740.6939.2637.6335.4922.4917.91

    5479.55917.8226.0728.0130.0526.34624.0116.5916.6916.915477.03911.5519.9440.8939.1239.6837.0734.9421.5817.69

    5489.35417.0925.4627.4629.5725.36823.616.2716.416.615486.81311.1419.2340.237.2338.2536.3934.4921.3817.26

    5499.08216.4824.9726.9728.925.123.1615.9416.1116.425496.67310.7618.6839.4436.5435.2635.923420.1516.97

    5508.75215.8424.3726.4728.224.98722.7315.5515.816.095506.42310.4718.0938.7336.4535.1935.0233.4119.2416.61

    5518.46815.1823.825.76527.6724.76522.2315.2415.4615.75516.1399.96217.5237.736.2335.0234.2932.719.5416.23

    5528.19914.5223.2524.78626.9924.21221.8714.9915.0115.285525.869.55916.9536.8335.2334.2633.3931.8328.5615.85

    5537.88413.922.824.59826.3823.68121.4114.6614.715.045535.6359.35416.4335.9235.1234.2632.6130.9218.4515.5

    5547.54113.3122.2324.37225.8522.98320.8114.2114.314.715545.4549.08215.6735.1134.2633.1631.6330.1418.2315.11

    5557.17312.5621.6223.15625.1222.57620.1813.7613.9514.35555.2428.75215.0534.1234.1232.1731.0529.2817.9814.73

    5566.89511.8821.0722.67824.1822.12919.6713.4113.5113.825565.0438.46814.4833.0733.6832.1930.1828.4817.4614.36

    5576.61711.2920.5522.57323.421.68319.1412.9713.1213.45574.7758.19913.932.2433.5631.8529.2927.716.8413.89

    5586.35810.6519.7622.12422.7221.36118.512.6412.6513.045584.5747.88413.3531.3132.4531.2728.4226.9816.3713.43

    5596.0610.2319.2321.54322.0220.12617.8212.2512.3112.725594.317.54112.7830.331.2130.5827.5326.115.3713.07

    5605.7399.82318.6221.34621.219.98217.1911.711.9312.35604.1417.17312.1629.2530.6730.2626.6525.2515.2612.72

    5615.3979.44217.8220.78420.5519.57216.6311.2911.511.85613.9446.89511.6128.1128.4527.8725.5524.2214.9512.25

    5625.1779.06317.0919.56219.7819.2115.9510.9111.0511.285623.7426.61711.0327.0928.1227.6324.5523.214.2511.71

    5634.8938.7916.4819.25618.918.3215.3710.4810.5510.785633.5246.35810.5326.2927.65426.4723.5322.5513.5711.23

    5644.6258.5215.8418.54618.2817.93614.79.93510.1910.45643.3256.069.96525.1226.4325.3722.6321.712.5710.83

    5654.3188.19215.1817.25917.4317.26114.099.4819.7099.9635653.1175.7399.36923.9922.5421.8921.7220.8812.3910.43

    5664.0647.90614.5216.12616.6316.113.499.0779.3319.4785662.8975.3978.78822.821.6820.9720.8219.9411.469.961

    5673.8587.62813.915.32515.8515.4212.88.7548.9379.025672.7155.1778.31721.8520.12520.0519.819.0210.269.492

    5683.5747.35713.3114.87515.1914.23512.268.4388.5518.6195682.5324.8937.8520.8719.4319.2619.0318.059.868.997

    5693.2937.19512.5614.2115.0113.58611.698.0278.1258.3085692.3674.6257.37319.9419.3418.7818.1317.299.568.602

    5703.0267.03911.8813.25814.28512.52411.097.5547.7237.8795702.2214.3186.9119.0118.6917.9617.2316.459.468.248

    5712.8676.81311.2912.45213.48311.5910.517.2427.3847.4475712.054.0646.5491818.3217.3616.3815.718.957.873

    5722.6646.67310.6511.25312.23511.419.9826.8716.9577.135721.8623.8586.13417.1217.3416.4915.5914.848.367.449

    5732.4486.42310.110.87511.85710.2359.4976.5176.5696.8055731.7343.5745.71516.3217.2316.0214.7514.18.166.995

    5742.2796.1399.60210.25411.2519.8569.036.1386.286.4975741.5993.2935.3215.5815.7815.2314.0713.527.346.727

    5752.0945.869.17310.12910.989.5328.5525.8795.9936.1295751.4763.0265.04614.8615.2314.9613.412.867.146.405

    5761.9135.6358.6619.87410.5839.1258.1515.585.6595.7865761.3522.8674.70814.0314.6813.4812.7512.16.976.061

    5771.7175.4548.2789.25110.1258.7347.6795.2755.3395.4775771.2212.6644.39613.3313.2313.0412.1111.456.875.756

    5781.615.2427.8438.9859.938.2317.3075.0045.1645.2245781.0792.4484.01912.6313.1212.9511.4210.896.7345.423

    5791.4765.0437.4158.1259.2318.16.9124.7824.8524.9675790.9772.2793.71611.9510.2310.2810.8610.346.635.178

    5801.3854.7757.0427.9238.8937.8566.6254.5694.6024.7085800.9042.0943.47611.310.110.0410.359.8926.454.904

    5811.2354.5746.6567.258.2517.2356.2434.2734.3794.4375810.841.9133.24710.699.459.659.8399.3536.214.668

    5821.1454.316.3026.8437.8326.8745.9444.0914.1034.1875820.7571.7173.04110.189.219.019.2718.8695.984.395

    5831.0394.1416.0356.5827.3516.3475.6373.8163.9323.9255830.7061.612.8279.6628.658.688.8728.5045.864.212

    5840.963.9445.7646.5216.9826.2365.3033.6633.7083.755840.6181.4762.6049.2478.238.678.5618.095.724.008

    5850.8663.7425.4516.2016.2385.8655.0583.4673.5423.6025850.5441.3852.4348.8648.548.468.1597.7685.633.802

    5860.7663.5245.1165.9836.0185.3464.7643.2813.3223.4095860.4941.2352.2848.47.237.947.7597.3785.123.601

    5870.6963.3254.8095.6215.7165.1254.4953.1253.1583.2315870.4221.1452.1178.0447.547.457.3686.9784.823.415

    5880.6453.1174.5935.215.4484.7454.3072.9953.0073.0615880.4011.0391.9977.6047.127.26.9886.6854.763.267

    5890.6232.8974.4764.8295.1464.4774.1072.8322.8292.9395890.3480.961.8457.2847.646.976.6666.2944.653.075

    5900.5482.7154.2654.6724.9474.1263.8952.7192.7452.7885900.3130.8661.6936.9436.436.876.3456.0524.522.905

    5910.482.5324.0274.3254.7333.9823.6672.552.6652.6155910.2550.7661.5566.6016.126.496.0745.7854.422.778

    5920.4142.3673.8284.2154.4293.5473.5152.3892.5372.4775920.1930.6961.466.3266.345.865.8155.494.322.635

    5930.3812.2213.6214.1854.2393.4563.4062.2942.4042.3285930.1630.6451.3776.1045.985.365.5515.2523.972.521

    5940.3082.053.4783.8274.0393.4213.2062.1862.2462.2235940.1520.6231.2955.85.765.2915.3315.0053.862.392

    5950.2751.8623.3363.7213.8863.13.0252.1082.1662.1435950.1060.5481.2285.485.235.125.0574.7483.752.266

    5960.2511.7343.1423.6123.6952.9152.8761.9752.042.0465960.0760.481.1525.224.764.964.8054.5333.692.177

    5970.2081.5992.9943.3153.4672.8652.7411.8471.9041.9615970.0470.4141.0674.9754.654.764.5524.3193.572.042

    5980.1711.4762.8473.2713.3122.7362.5631.7461.81.8325980.0230.3810.9814.7084.124.264.3144.0693.451.939

    5990.1361.3522.7083.1853.1482.6352.4111.7031.6771.7285990.0090.3080.8954.5293.983.924.1173.8863.271.835

    6000.0921.2212.5342.853.012.5192.3031.5821.6021.656000.0020.2750.8164.2813.763.653.8633.6792.851.775

    Sheet2

    Sheet3

  • Fluorescent Intensity Released by Nanocapsules in the Absence of Light

    Chart1

    0000.818

    0000.828

    0000.8

    0000.878

    0000.906

    0000.969

    0001.027

    0001.102

    0001.227

    0001.371

    0001.51

    0001.714

    0001.946

    0002.195

    0002.468

    000.0782.645

    00.1250.2113.122

    00.2540.3683.751

    00.4370.5764.484

    0.0070.5360.7995.328

    0.0530.9321.116.193

    0.1611.3461.4337.324

    0.2541.8211.8358.49

    0.4022.1992.3259.818

    0.622.6272.95911.35

    0.8753.0963.6813.08

    1.1583.5964.51115.15

    1.4984.2865.47117.4

    1.9275.0186.61619.62

    2.4175.858.04822

    2.9866.8429.63824.74

    3.6087.98811.4127.61

    4.329.0513.530.57

    5.17410.315.6933.55

    5.9811.8118.0436.34

    6.94113.3520.6739.36

    8.04415.123.5342.18

    9.12616.7526.6745.15

    10.3618.4629.9347.76

    11.6720.2733.250.45

    12.921.9136.5652.56

    14.1423.5239.8654.81

    15.3325.4943.157.3

    16.6227.3146.1759.21

    17.6528.9248.5660.65

    18.6930.5651.2761.74

    19.6732.1253.6462.84

    20.3933.2555.2563.75

    20.8933.56757.0764.47

    21.434.26758.2364.66

    21.7534.67859.0664.73

    21.9435.12659.4464.64

    22.0535.6159.7764.63

    22.0535.23659.5564.26

    22.135.159.1963.98

    21.8334.12658.7263.3

    21.633.6257.7762.65

    21.233.29656.361.78

    20.7932.0555.1461.24

    20.2631.6553.5960.47

    19.6831.1951.9659.53

    19.1330.8350.6658.56

    18.4730.1949.1357.43

    17.9829.5647.3856.58

    17.3728.7745.5355.85

    16.6727.944.0754.98

    15.9826.9242.2554.1

    15.3326.0240.4953.08

    14.6725.0638.8852.35

    13.9924.0837.2751.58

    13.423.1935.7650.88

    12.7822.1434.3950.05

    12.2121.2533.1549.41

    11.7220.4131.948.74

    11.1219.6230.5548.2

    10.6418.7329.3847.69

    10.2317.9228.3147.34

    9.82317.1627.446.77

    9.44216.4726.3346.31

    9.06315.8125.4545.7

    8.7915.1224.6245.21

    8.5214.5223.8444.68

    8.19214.0223.0544

    7.90613.3722.3743.58

    7.62812.8821.7542.97

    7.35712.3821.0342.34

    7.19511.9820.4841.67

    7.03911.5519.9440.89

    6.81311.1419.2340.2

    6.67310.7618.6839.44

    6.42310.4718.0938.73

    6.1399.96217.5237.7

    5.869.55916.9536.83

    5.6359.35416.4335.92

    5.4549.08215.6735.11

    5.2428.75215.0534.12

    5.0438.46814.4833.07

    4.7758.19913.932.24

    4.5747.88413.3531.31

    4.317.54112.7830.3

    4.1417.17312.1629.25

    3.9446.89511.6128.11

    3.7426.61711.0327.09

    3.5246.35810.5326.29

    3.3256.069.96525.12

    3.1175.7399.36923.99

    2.8975.3978.78822.8

    2.7155.1778.31721.85

    2.5324.8937.8520.87

    2.3674.6257.37319.94

    2.2214.3186.9119.01

    2.054.0646.54918

    1.8623.8586.13417.12

    1.7343.5745.71516.32

    1.5993.2935.3215.58

    1.4763.0265.04614.86

    1.3522.8674.70814.03

    1.2212.6644.39613.33

    1.0792.4484.01912.63

    0.9772.2793.71611.95

    0.9042.0943.47611.3

    0.841.9133.24710.69

    0.7571.7173.04110.18

    0.7061.612.8279.662

    0.6181.4762.6049.247

    0.5441.3852.4348.864

    0.4941.2352.2848.4

    0.4221.1452.1178.044

    0.4011.0391.9977.604

    0.3480.961.8457.284

    0.3130.8661.6936.943

    0.2550.7661.5566.601

    0.1930.6961.466.326

    0.1630.6451.3776.104

    0.1520.6231.2955.8

    0.1060.5481.2285.48

    0.0760.481.1525.22

    0.0470.4141.0674.975

    0.0230.3810.9814.708

    0.0090.3080.8954.529

    0.0020.2750.8164.281

    Intensity after 5 min

    Intensity after 10 min

    Intensity after 15 min

    Intensity after 20 min

    Wavelength (nm)

    Fluorescent Intensity

    Fluorescent Intensity Released by Nanocapsules Energized by UV Light

    Chart2

    000.2360.8180.952

    000.2460.8281.24

    000.2510.81.235

    000.2440.8781.643

    000.2690.8951.782

    00.1340.3120.9691.709

    00.2450.3510.9951.923

    00.2780.3871.1022.352

    00.3460.461.2272.63

    00.480.5521.3712.873

    00.9340.6561.512.832

    01.1450.791.7142.965

    01.2580.9211.9462.987

    01.451.1232.1953.012

    01.3781.3742.4683.784

    01.4561.6632.8583.926

    01.6681.9753.3494.324

    01.7682.4353.8544.567

    01.8352.8854.4745.432

    0.0712.5463.355.1785.632

    0.2132.7683.9956.0826.125

    0.3562.8754.7537.0497.432

    0.522.9875.6268.2028.346

    0.7453.0116.4828.5549.231

    1.014.5637.6189.8489.658

    1.3594.6788.7411.313.235

    1.8024.8799.94613.0415.57

    2.3285.32611.4814.8515.687

    2.9386.43213.1516.9318.325

    3.6167.45614.941919.543

    4.5129.45316.620.8921.1

    5.45710.23418.4321.421.25

    6.53112.47520.3522.5423.54

    7.69714.57222.2623.4325.71

    8.93715.567224.125.4327.87

    10.3517.28325.9926.32530.17

    11.9218.567827.8629.4532.41

    13.5820.23529.7931.2334.6

    15.3122.47331.6833.25636.92

    17.224.83833.2335.1238.85

    18.9426.478334.5935.67840.51

    20.7628.5436.137.12542.2

    22.3632.56737.2639.2543.55

    24.2633.567238.5440.2344.79

    25.834.562739.5941.68545.82

    27.2835.67840.1542.3846.76

    28.4436.12540.7542.987447.51

    29.5136.83741.2643.78548.04

    30.2537.1241.5943.97548.53

    31.0437.98741.8844.1548.69

    31.4338.01242.0244.3248.91

    31.9138.3742.0144.4348.63

    32.138.12541.944.6148.21

    32.0537.987741.7544.2348.02

    31.6537.45241.2843.76547.72

    31.1936.89540.9142.96247.44

    30.8335.5640.6142.56346.66

    30.1934.675340.0542.1246.07

    29.5633.98739.4241.65745.38

    28.7732.85438.7440.49844.66

    27.932.36438.1640.21543.95

    26.9231.59637.6939.25843.19

    26.0231.27437.0739.142.53

    25.0630.48636.4238.4341.68

    24.0829.9835.8337.8441.1

    23.1929.12535.2437.2140.53

    22.1428.45834.5436.5439.96

    21.2528.12534.0436.239.1

    20.4127.68533.3335.4238.52

    19.6227.12533.0735.2138.2

    18.7326.98432.634.7537.62

    17.9226.34132.2934.5636.97

    17.1625.87431.8533.4536.43

    16.4724.9331.2733.2536.04

    15.8124.5130.8432.8435.69

    15.1224.0130.5232.5135.52

    14.5223.630.1532.1535.02

    14.0223.1629.8431.8434.55

    13.3722.7329.5731.5434.3

    12.8822.2329.2431.2533.96

    12.3821.8728.9730.9533.39

    11.9821.4128.530.5432.97

    11.5520.8128.1430.1232.6

    11.1420.1827.5829.56732.08

    10.7620.4127.2229.2231.74

    10.4719.6226.7928.7531.17

    9.96218.6226.6328.6330.43

    9.55917.8226.0728.0130.05

    9.35417.0925.4627.4629.57

    9.08216.4824.9726.9728.9

    8.75215.8424.3726.4728.2

    8.46815.1823.825.76527.67

    8.19914.5223.2524.78626.99

    7.88413.922.824.59826.38

    7.54113.3122.2324.37225.85

    7.17312.5621.6223.15625.12

    6.89511.8821.0722.67824.18

    6.61711.2920.5522.57323.4

    6.35810.6519.7622.12422.72

    6.0610.2319.2321.54322.02

    5.7399.82318.6221.34621.2

    5.3979.44217.8220.78420.55

    5.1779.06317.0919.56219.78

    4.8938.7916.4819.25618.9

    4.6258.5215.8418.54618.28

    4.3188.19215.1817.25917.43

    4.0647.90614.5216.12616.63

    3.8587.62813.915.32515.85

    3.5747.35713.3114.87515.19

    3.2937.19512.5614.2115.01

    3.0267.03911.8813.25814.285

    2.8676.81311.2912.45213.483

    2.6646.67310.6511.25312.235

    2.4486.42310.110.87511.857

    2.2796.1399.60210.25411.251

    2.0945.869.17310.12910.98

    1.9135.6358.6619.87410.583

    1.7175.4548.2789.25110.125

    1.615.2427.8438.9859.93

    1.4765.0437.4158.1259.231

    1.3854.7757.0427.9238.893

    1.2354.5746.6567.258.251

    1.1454.316.3026.8437.832

    1.0394.1416.0356.5827.351

    0.963.9445.7646.5216.982

    0.8663.7425.4516.2016.238

    0.7663.5245.1165.9836.018

    0.6963.3254.8095.6215.716

    0.6453.1174.5935.215.448

    0.6232.8974.4764.8295.146

    0.5482.7154.2654.6724.947

    0.482.5324.0274.3254.733

    0.4142.3673.8284.2154.429

    0.3812.2213.6214.1854.239

    0.3082.053.4783.8274.039

    0.2751.8623.3363.7213.886

    0.2511.7343.1423.6123.695

    0.2081.5992.9943.3153.467

    0.1711.4762.8473.2713.312

    0.1361.3522.7083.1853.148

    0.0921.2212.5342.853.01

    Intensity after 5 min

    Intensity after 10 min

    Intensity after 15 min

    Intensity after 20 min

    Intensity after 25 min

    Wavelength (nm)

    Fluorescent Intensity

    Chart3

    32.122.1

    38.3735.61

    42.0259.77

    44.6164.73

    48.9164.23

    45.3263.32

    39.1458.72

    26.8755.28

    26.2147.68

    26.0827.63

    Nanocapsules under the absence of light

    Nanocapsules energized by UV light

    Time (min)

    Fluorescent Intensity

    Fluorescent Intensity Released by Nanocapsules over Time

    Chart4

    32.122.1

    38.3735.61

    42.0259.77

    44.6164.73

    48.9120

    In the Absence of Light

    Energized by UV Light

    Time (min)

    Fluorescent Intensity

    Increasing Fluorescent Intensity Released by Nanocapsules over Time

    Sheet1

    DK1UV1TimePeaks

    Data PointsData PointsDKUVDKUV

    nmDataDataDataDataDataDataDatanmDataDataDataDataDataData032.122.122.9824.12

    460000.2360.8180.9520.8530.3715.4575.1644.6854600000.8180.8010.7940.3610.3510.3710.236538.3735.6126.6929.31

    461000.2460.8281.240.9340.3995.3595.1614.4894610000.8280.8670.8120.3790.3670.3990.2461042.0259.7727.729.6

    462000.2510.81.2350.9520.3554.7494.7284.0284620000.80.8430.8250.3930.3870.3550.2511544.6164.7329.632.19

    463000.2440.8781.6431.1560.3564.0224.0453.4224630000.8780.8310.8340.4260.4150.3560.2442048.9164.2330.447.75

    464000.2690.8951.7821.2560.3553.4083.3822.8714640000.9060.8920.8660.4820.4260.3550.2692545.3263.3234.640.32

    46500.1340.3120.9691.7091.3560.3532.8632.8432.3854650000.9690.9350.9120.570.4860.3530.3123039.1458.7227.833.21

    46600.2450.3510.9951.9231.5720.3762.4882.4582.074660001.0270.9750.9430.6690.5830.3760.3516026.8755.2823.6227.67

    46700.2780.3871.1022.3521.6920.4282.3222.2241.9224670001.1021.0480.9650.7580.7230.4280.38712026.2147.6820.4524.68

    46800.3460.461.2272.631.9280.4972.2242.1151.8714680001.2271.1531.2560.8540.7930.4970.4624026.0827.6319.2319.16

    46900.480.5521.3712.8732.0510.5522.1692.0791.8944690001.3711.2251.3460.9860.8950.5520.552

    47000.9340.6561.512.8322.1560.6182.1081.9951.8444700001.511.361.8671.1591.0450.6180.656

    47101.1450.791.7142.9652.2560.7412.0511.9731.7634710001.7141.511.6731.3521.2450.7410.79

    47201.2580.9211.9462.9872.4160.8652.061.9281.7994720001.9461.7541.6921.5681.3560.8650.921

    47301.451.1232.1953.0122.7121.0432.1281.9781.8734730002.1951.9761.8761.8441.5861.0431.123

    47401.3781.3742.4683.7842.8361.2432.1532.0131.9574740002.4682.2472.1252.2391.6791.2431.374032.122.1

    47501.4561.6632.8583.9263.2361.5342.1972.0552.012475000.0782.6452.6332.5462.8582.5981.5341.663538.3735.61

    47601.6681.9753.3494.3243.5671.8212.2812.1222.12347600.1250.2113.1223.2363.1253.3493.0631.8211.9751042.0259.77

    47701.7682.4353.8544.5673.6322.1992.4762.3162.2947700.2540.3683.7513.5673.2353.8543.5612.1992.4351544.6164.73

    47801.8352.8854.4745.4323.7352.6272.6762.5222.50947800.4370.5764.4844.3264.1294.4744.0962.6272.8852048.91

    4790.0712.5463.355.1785.6324.2163.0962.9322.7392.7754790.0070.5360.7995.3285.2314.685.1784.7343.0963.5625

    4800.2132.7683.9956.0826.1254.4563.5963.2943.1043.1974800.0530.9321.116.1936.2375.436.0825.4693.5963.96

    4810.3562.8754.7537.0497.4325.2364.2863.7643.5273.6584810.1611.3461.4337.3247.2126.977.0496.4194.2864.097

    4820.522.9875.6268.2028.3465.6325.0184.2563.9844.174820.2541.8211.8358.498.3627.348.2027.4375.0184.526

    4830.7453.0116.4828.5549.2316.3255.854.7694.5414.7164830.4022.1992.3259.8189.7238.459.4458.5545.855.141

    4841.014.5637.6189.8489.6586.8926.8425.3765.1525.3934840.622.6272.95911.3510.3269.23410.839.8486.8425.728

    4851.3594.6788.7411.313.2358.3467.9886.1025.8166.1224850.8753.0963.6813.0811.23510.2312.3911.37.7626.394

    4861.8024.8799.94613.0415.579.2369.056.8126.5346.9054861.1583.5964.51115.1515.23614.2314.1813.048.6687.16

    4872.3285.32611.4814.8515.68710.23610.37.6057.3597.7624871.4984.2865.47117.416.23615.23616.2214.8511.428.014

    4882.9386.43213.1516.9318.32512.36211.818.4748.3118.6684881.9275.0186.61619.6218.23616.2318.2816.9313.258.936

    4893.6167.45614.941919.54315.23613.359.4429.2199.6284892.4175.858.0482221.2520.4320.531915.949.855

    4904.5129.45316.620.8921.115.89215.110.5110.2810.734902.9866.8429.63824.7424.53623.6522.8521.0818.35210.99

    4915.45710.23418.4321.421.2518.32616.7511.5911.3611.914913.6087.98811.4127.6126.23525.3425.4823.2519.5412.18

    4926.53112.47520.3522.5423.5421.23618.4612.8112.5913.124924.329.0513.530.5729.12628.5628.0225.8121.5313.35

    4937.69714.57222.2623.4325.7121.32620.2713.9813.7714.244935.17410.315.6933.5531.23630.6530.728.3524.3514.6

    4948.93715.567224.125.4327.8722.56121.9115.1314.8915.414945.9811.8118.0436.3432.36231.2533.3230.9128.43515.71

    49510.3517.28325.9926.32530.1724.26123.5216.2515.9916.644956.94113.3520.6739.3635.235.6435.7733.2329.3216.95

    49611.9218.567827.8629.4532.4125.98625.4917.4917.1517.764968.04415.123.5342.1839.12538.4538.4535.7731.2518.14

    49713.5820.23529.7931.2334.627.89327.3118.5718.3319.064979.12616.7526.6745.1542.12541.2341.0338.2534.2519.24

    49815.3122.47331.6833.25636.9230.2628.9219.619.4420.0749810.3618.4629.9347.7645.23642.6543.5340.5135.4220.35

    49917.224.83833.2335.1238.8535.21630.5620.5720.4421.0849911.6720.2733.250.4549.23648.2345.7542.7236.3321.4

    50018.9426.478334.5935.67840.5138.26132.1221.4321.2721.9850012.921.9136.5652.5651.2651.6747.9344.6437.122.33

    50120.7628.5436.137.12542.240.63733.2522.3222.0422.9950114.1423.5239.8654.8153.23652.6849.846.5737.8423.27

    50222.3632.56737.2639.2543.5540.98234.2923.2322.8323.7350215.3325.4943.157.358.43655.3251.7348.1838.3224.29

    50324.2633.567238.5440.2344.7941.26935.4223.9723.6524.4650316.6227.3146.1759.2159.23656.5453.2349.8638.6525.02

    50425.834.562739.5941.68545.8241.64836.3324.5724.1925.1350417.6528.9248.5660.6560.76558.7854.651.0741.9225.72

    50527.2835.67840.1542.3846.7642.69437.125.1724.7125.6150518.6930.5651.2761.7461.32659.2355.8852.1742.5626.26

    50628.4436.12540.7542.987447.5143.99737.8425.4525.0926.1650619.6732.1253.6462.8462.36560.3456.7753.0143.6726.66

    50729.5136.83741.2643.78548.0444.02538.3225.6425.426.450720.3933.2555.2563.7563.67861.2457.3553.7844.5327.06

    50830.2537.1241.5943.97548.5344.72138.6525.9525.8426.7250820.8933.56757.0764.4763.98661.4957.8754.3846.4327.25

    50931.0437.98741.8844.1548.6945.02139.0426.1426.0826.8250921.434.26758.2364.6664.12562.4558.3354.7846.9827.43

    51031.4338.01242.0244.3248.9145.3239.1426.8726.2126.8451021.7534.67859.0664.7364.21962.7858.4455.1847.2627.49

    51131.9138.3742.0144.4348.6345.20639.1126.0326.126.0851121.9435.12659.4464.6464.2363.3258.6755.2847.6827.63

    51232.138.12541.944.6148.2144.21638.9325.9925.9926.7651222.0535.6159.7764.6363.26162.3458.5655.1746.9427.66

    51332.0537.987741.7544.2348.0243.65738.6126.0225.8826.751322.0535.23659.5564.2663.12962.1958.2654.845.6827.42

    51431.6537.45241.2843.76547.7243.19538.2525.9125.7326.4851422.135.159.1963.9862.31261.2357.6354.5445.2327.18

    51531.1936.89540.9142.96247.4442.17537.9125.5525.5226.2951521.8334.12658.7263.360.23160.1657.2553.8744.7527.03

    51630.8335.5640.6142.56346.6641.92437.6825.3125.2625.9551621.633.6257.7762.6561.23959.5656.5753.243.2626.95

    51730.1934.675340.0542.1246.0741.82737.3124.9224.9425.6151721.233.29656.361.7860.98259.2355.9252.6842.6926.61

    51829.5633.98739.4241.65745.3841.79536.5824.7124.7525.2851820.7932.0555.1461.2460.84358.9655.2152.0941.2826.28

    51928.7732.85438.7440.49844.6640.87536.1324.3624.5125.0451920.2631.6553.5960.4759.23658.7554.4951.4741.125.9

    52027.932.36438.1640.21543.9540.67235.4823.8223.9424.6152019.6831.1951.9659.5359.126658.2353.6750.6940.4625.53

    52126.9231.59637.6939.25843.1940.51834.8323.3623.5824.4152119.1330.8350.6658.5658.32857.3452.9949.9139.5125.1

    52226.0231.27437.0739.142.5339.7434.3123.0423.3123.8552218.4730.1949.1357.4357.65456.4652.0949.2638.3524.64

    52325.0630.48636.4238.4341.6839.47133.8422.6722.8723.4452317.9829.5647.3856.5856.23856.2451.1948.4837.2624.18

    52424.0829.9835.8337.8441.137.57233.322.422.623.1452417.3728.7745.5355.8555.12655.6750.4447.5736.5823.66

    52523.1929.12535.2437.2140.5337.19232.7722.0222.2322.6952516.6727.944.0754.9853.68254.2349.8746.935.4123.26

    52622.1428.45834.5436.5439.9636.38332.221.6721.922.4152615.9826.9242.2554.154.86753.1649.1146.1634.3822.99

    52721.2528.12534.0436.239.135.47231.7521.3421.5622.1452715.3326.0240.4953.0852.12852.3548.3945.5833.2722.65

    52820.4127.68533.3335.4238.5234.32831.2421.0621.3721.8452814.6725.0638.8852.3551.23651.9647.6344.8632.9622.4

    52919.6227.12533.0735.2138.234.12830.7920.8821.0621.4852913.9924.0837.2751.5850.23649.2646.7644.1932.7522.17

    53018.7326.98432.634.7537.6233.82530.3620.5820.7321.1753013.423.1935.7650.8849.23648.2345.9843.5631.5721.89

    53117.9226.34132.2934.5636.9733.72630.0220.3620.4121.0453112.7822.1434.3950.0549.3248.1545.5242.9830.2721.51

    53217.1625.87431.8533.4536.4333.46129.6620.0320.1820.8853212.2121.2533.1549.4148.9647.2344.8442.3730.4621.23

    53316.4724.9331.2733.2536.0432.15629.1819.7719.9220.5753311.7220.4131.948.7448.1247.7844.4241.9329.5721.14

    53415.8124.5130.8432.8435.6932.01228.7519.4819.7720.2853411.1219.6230.5548.247.1346.7843.9441.4129.3420.81

    53515.1224.0130.5232.5135.5231.87628.4719.1919.5720.1353510.6418.7329.3847.6946.8946.2443.5340.9428.4520.55

    53614.5223.630.1532.1535.0231.73228.1419.1119.2319.8453610.2317.9228.3147.3446.5745.6842.8840.628.1420.29

    53714.0223.1629.8431.8434.5531.57227.6818.8518.9519.45379.82317.1627.446.7745.2145.3642.4940.227.5620.15

    53813.3722.7329.5731.5434.330.57127.418.7518.7619.245389.44216.4726.3346.3145.1244.1241.8139.7527.4319.85

    53912.8822.2329.2431.2533.9630.25127.0618.518.6619.155399.06315.8125.4545.744.6743.2641.3339.1426.5819.69

    54012.3821.8728.9730.9533.3929.78526.6718.1918.3718.925408.7915.1224.6245.2144.2343.1640.9838.6626.3619.46

    54111.9821.4128.530.5432.9728.5626.3817.9318.2418.685418.5214.5223.8444.6843.1242.6740.5138.2425.3819.21

    54211.5520.8128.1430.1232.628.45626.0817.7817.8718.475428.19214.0223.054442.6742.3639.9237.7525.118.94

    54311.1420.1827.5829.56732.0827.34525.7417.5917.6218.235437.90613.3722.3743.5842.1241.6739.3237.2624.7318.6

    54410.7620.4127.2229.2231.7427.12925.3317.3417.5117.935447.62812.8821.7542.9741.8641.2938.9436.6324.1618.38

    54510.4719.6226.7928.7531.1726.89524.9317.0817.2917.75457.35712.3821.0342.3441.5640.2638.2936.0623.4718.19

    5469.96218.6226.6328.6330.4326.78424.5116.781717.45467.19511.9820.4841.6740.6939.2637.6335.4922.4917.91

    5479.55917.8226.0728.0130.0526.34624.0116.5916.6916.915477.03911.5519.9440.8939.1239.6837.0734.9421.5817.69

    5489.35417.0925.4627.4629.5725.36823.616.2716.416.615486.81311.1419.2340.237.2338.2536.3934.4921.3817.26

    5499.08216.4824.9726.9728.925.123.1615.9416.1116.425496.67310.7618.6839.4436.5435.2635.923420.1516.97

    5508.75215.8424.3726.4728.224.98722.7315.5515.816.095506.42310.4718.0938.7336.4535.1935.0233.4119.2416.61

    5518.46815.1823.825.76527.6724.76522.2315.2415.4615.75516.1399.96217.5237.736.2335.0234.2932.719.5416.23

    5528.19914.5223.2524.78626.9924.21221.8714.9915.0115.285525.869.55916.9536.8335.2334.2633.3931.8328.5615.85

    5537.88413.922.824.59826.3823.68121.4114.6614.715.045535.6359.35416.4335.9235.1234.2632.6130.9218.4515.5

    5547.54113.3122.2324.37225.8522.98320.8114.2114.314.715545.4549.08215.6735.1134.2633.1631.6330.1418.2315.11

    5557.17312.5621.6223.15625.1222.57620.1813.7613.9514.35555.2428.75215.0534.1234.1232.1731.0529.2817.9814.73

    5566.89511.8821.0722.67824.1822.12919.6713.4113.5113.825565.0438.46814.4833.0733.6832.1930.1828.4817.4614.36

    5576.61711.2920.5522.57323.421.68319.1412.9713.1213.45574.7758.19913.932.2433.5631.8529.2927.716.8413.89

    5586.35810.6519.7622.12422.7221.36118.512.6412.6513.045584.5747.88413.3531.3132.4531.2728.4226.9816.3713.43

    5596.0610.2319.2321.54322.0220.12617.8212.2512.3112.725594.317.54112.7830.331.2130.5827.5326.115.3713.07

    5605.7399.82318.6221.34621.219.98217.1911.711.9312.35604.1417.17312.1629.2530.6730.2626.6525.2515.2612.72

    5615.3979.44217.8220.78420.5519.57216.6311.2911.511.85613.9446.89511.6128.1128.4527.8725.5524.2214.9512.25

    5625.1779.06317.0919.56219.7819.2115.9510.9111.0511.285623.7426.61711.0327.0928.1227.6324.5523.214.2511.71

    5634.8938.7916.4819.25618.918.3215.3710.4810.5510.785633.5246.35810.5326.2927.65426.4723.5322.5513.5711.23

    5644.6258.5215.8418.54618.2817.93614.79.93510.1910.45643.3256.069.96525.1226.4325.3722.6321.712.5710.83

    5654.3188.19215.1817.25917.4317.26114.099.4819.7099.9635653.1175.7399.36923.9922.5421.8921.7220.8812.3910.43

    5664.0647.90614.5216.12616.6316.113.499.0779.3319.4785662.8975.3978.78822.821.6820.9720.8219.9411.469.961

    5673.8587.62813.915.32515.8515.4212.88.7548.9379.025672.7155.1778.31721.8520.12520.0519.819.0210.269.492

    5683.5747.35713.3114.87515.1914.23512.268.4388.5518.6195682.5324.8937.8520.8719.4319.2619.0318.059.868.997

    5693.2937.19512.5614.2115.0113.58611.698.0278.1258.3085692.3674.6257.37319.9419.3418.7818.1317.299.568.602

    5703.0267.03911.8813.25814.28512.52411.097.5547.7237.8795702.2214.3186.9119.0118.6917.9617.2316.459.468.248

    5712.8676.81311.2912.45213.48311.5910.517.2427.3847.4475712.054.0646.5491818.3217.3616.3815.718.957.873

    5722.6646.67310.6511.25312.23511.419.9826.8716.9577.135721.8623.8586.13417.1217.3416.4915.5914.848.367.449

    5732.4486.42310.110.87511.85710.2359.4976.5176.5696.8055731.7343.5745.71516.3217.2316.0214.7514.18.166.995

    5742.2796.1399.60210.25411.2519.8569.036.1386.286.4975741.5993.2935.3215.5815.7815.2314.0713.527.346.727

    5752.0945.869.17310.12910.989.5328.5525.8795.9936.1295751.4763.0265.04614.8615.2314.9613.412.867.146.405

    5761.9135.6358.6619.87410.5839.1258.1515.585.6595.7865761.3522.8674.70814.0314.6813.4812.7512.16.976.061

    5771.7175.4548.2789.25110.1258.7347.6795.2755.3395.4775771.2212.6644.39613.3313.2313.0412.1111.456.875.756

    5781.615.2427.8438.9859.938.2317.3075.0045.1645.2245781.0792.4484.01912.6313.1212.9511.4210.896.7345.423

    5791.4765.0437.4158.1259.2318.16.9124.7824.8524.9675790.9772.2793.71611.9510.2310.2810.8610.346.635.178

    5801.3854.7757.0427.9238.8937.8566.6254.5694.6024.7085800.9042.0943.47611.310.110.0410.359.8926.454.904

    5811.2354.5746.6567.258.2517.2356.2434.2734.3794.4375810.841.9133.24710.699.459.659.8399.3536.214.668

    5821.1454.316.3026.8437.8326.8745.9444.0914.1034.1875820.7571.7173.04110.189.219.019.2718.8695.984.395

    5831.0394.1416.0356.5827.3516.3475.6373.8163.9323.9255830.7061.612.8279.6628.658.688.8728.5045.864.212

    5840.963.9445.7646.5216.9826.2365.3033.6633.7083.755840.6181.4762.6049.2478.238.678.5618.095.724.008

    5850.8663.7425.4516.2016.2385.8655.0583.4673.5423.6025850.5441.3852.4348.8648.548.468.1597.7685.633.802

    5860.7663.5245.1165.9836.0185.3464.7643.2813.3223.4095860.4941.2352.2848.47.237.947.7597.3785.123.601

    5870.6963.3254.8095.6215.7165.1254.4953.1253.1583.2315870.4221.1452.1178.0447.547.457.3686.9784.823.415

    5880.6453.1174.5935.215.4484.7454.3072.9953.0073.0615880.4011.0391.9977.6047.127.26.9886.6854.763.267

    5890.6232.8974.4764.8295.1464.4774.1072.8322.8292.9395890.3480.961.8457.2847.646.976.6666.2944.653.075

    5900.5482.7154.2654.6724.9474.1263.8952.7192.7452.7885900.3130.8661.6936.9436.436.876.3456.0524.522.905

    5910.482.5324.0274.3254.7333.9823.6672.552.6652.6155910.2550.7661.5566.6016.126.496.0745.7854.422.778

    5920.4142.3673.8284.2154.4293.5473.5152.3892.5372.4775920.1930.6961.466.3266.345.865.8155.494.322.635

    5930.3812.2213.6214.1854.2393.4563.4062.2942.4042.3285930.1630.6451.3776.1045.985.365.5515.2523.972.521

    5940.3082.053.4783.8274.0393.4213.2062.1862.2462.2235940.1520.6231.2955.85.765.2915.3315.0053.862.392

    5950.2751.8623.3363.7213.8863.13.0252.1082.1662.1435950.1060.5481.2285.485.235.125.0574.7483.752.266

    5960.2511.7343.1423.6123.6952.9152.8761.9752.042.0465960.0760.481.1525.224.764.964.8054.5333.692.177

    5970.2081.5992.9943.3153.4672.8652.7411.8471.9041.9615970.0470.4141.0674.9754.654.764.5524.3193.572.042

    5980.1711.4762.8473.2713.3122.7362.5631.7461.81.8325980.0230.3810.9814.7084.124.264.3144.0693.451.939

    5990.1361.3522.7083.1853.1482.6352.4111.7031.6771.7285990.0090.3080.8954.5293.983.924.1173.8863.271.835

    6000.0921.2212.5342.853.012.5192.3031.5821.6021.656000.0020.2750.8164.2813.763.653.8633.6792.851.775

    Sheet2

    Sheet3

  • Increasing Fluorescent Intensity Released by Nanocapsules over Time

    Chart1

    0000.818

    0000.828

    0000.8

    0000.878

    0000.906

    0000.969

    0001.027

    0001.102

    0001.227

    0001.371

    0001.51

    0001.714

    0001.946

    0002.195

    0002.468

    000.0782.645

    00.1250.2113.122

    00.2540.3683.751

    00.4370.5764.484

    0.0070.5360.7995.328

    0.0530.9321.116.193

    0.1611.3461.4337.324

    0.2541.8211.8358.49

    0.4022.1992.3259.818

    0.622.6272.95911.35

    0.8753.0963.6813.08

    1.1583.5964.51115.15

    1.4984.2865.47117.4

    1.9275.0186.61619.62

    2.4175.858.04822

    2.9866.8429.63824.74

    3.6087.98811.4127.61

    4.329.0513.530.57

    5.17410.315.6933.55

    5.9811.8118.0436.34

    6.94113.3520.6739.36

    8.04415.123.5342.18

    9.12616.7526.6745.15

    10.3618.4629.9347.76

    11.6720.2733.250.45

    12.921.9136.5652.56

    14.1423.5239.8654.81

    15.3325.4943.157.3

    16.6227.3146.1759.21

    17.6528.9248.5660.65

    18.6930.5651.2761.74

    19.6732.1253.6462.84

    20.3933.2555.2563.75

    20.8933.56757.0764.47

    21.434.26758.2364.66

    21.7534.67859.0664.73

    21.9435.12659.4464.64

    22.0535.6159.7764.63

    22.0535.23659.5564.26

    22.135.159.1963.98

    21.8334.12658.7263.3

    21.633.6257.7762.65

    21.233.29656.361.78

    20.7932.0555.1461.24

    20.2631.6553.5960.47

    19.6831.1951.9659.53

    19.1330.8350.6658.56

    18.4730.1949.1357.43

    17.9829.5647.3856.58

    17.3728.7745.5355.85

    16.6727.944.0754.98

    15.9826.9242.2554.1

    15.3326.0240.4953.08

    14.6725.0638.8852.35

    13.9924.0837.2751.58

    13.423.1935.7650.88

    12.7822.1434.3950.05

    12.2121.2533.1549.41

    11.7220.4131.948.74

    11.1219.6230.5548.2

    10.6418.7329.3847.69

    10.2317.9228.3147.34

    9.82317.1627.446.77

    9.44216.4726.3346.31

    9.06315.8125.4545.7

    8.7915.1224.6245.21

    8.5214.5223.8444.68

    8.19214.0223.0544

    7.90613.3722.3743.58

    7.62812.8821.7542.97

    7.35712.3821.0342.34

    7.19511.9820.4841.67

    7.03911.5519.9440.89

    6.81311.1419.2340.2

    6.67310.7618.6839.44

    6.42310.4718.0938.73

    6.1399.96217.5237.7

    5.869.55916.9536.83

    5.6359.35416.4335.92

    5.4549.08215.6735.11

    5.2428.75215.0534.12

    5.0438.46814.4833.07

    4.7758.19913.932.24

    4.5747.88413.3531.31

    4.317.54112.7830.3

    4.1417.17312.1629.25

    3.9446.89511.6128.11

    3.7426.61711.0327.09

    3.5246.35810.5326.29

    3.3256.069.96525.12

    3.1175.7399.36923.99

    2.8975.3978.78822.8

    2.7155.1778.31721.85

    2.5324.8937.8520.87

    2.3674.6257.37319.94

    2.2214.3186.9119.01

    2.054.0646.54918

    1.8623.8586.13417.12

    1.7343.5745.71516.32

    1.5993.2935.3215.58

    1.4763.0265.04614.86

    1.3522.8674.70814.03

    1.2212.6644.39613.33

    1.0792.4484.01912.63

    0.9772.2793.71611.95

    0.9042.0943.47611.3

    0.841.9133.24710.69

    0.7571.7173.04110.18

    0.7061.612.8279.662

    0.6181.4762.6049.247

    0.5441.3852.4348.864

    0.4941.2352.2848.4

    0.4221.1452.1178.044

    0.4011.0391.9977.604

    0.3480.961.8457.284

    0.3130.8661.6936.943

    0.2550.7661.5566.601

    0.1930.6961.466.326

    0.1630.6451.3776.104

    0.1520.6231.2955.8

    0.1060.5481.2285.48

    0.0760.481.1525.22

    0.0470.4141.0674.975

    0.0230.3810.9814.708

    0.0090.3080.8954.529

    0.0020.2750.8164.281

    Intensity after 5 min

    Intensity after 10 min

    Intensity after 15 min

    Intensity after 20 min

    Wavelength (nm)

    Fluorescent Intensity

    Fluorescent Intensity Released by Nanocapsules Energized by UV Light

    Chart2

    000.2360.8180.952

    000.2460.8281.24

    000.2510.81.235

    000.2440.8781.643

    000.2690.8951.782

    00.1340.3120.9691.709

    00.2450.3510.9951.923

    00.2780.3871.1022.352

    00.3460.461.2272.63

    00.480.5521.3712.873

    00.9340.6561.512.832

    01.1450.791.7142.965

    01.2580.9211.9462.987

    01.451.1232.1953.012

    01.3781.3742.4683.784

    01.4561.6632.8583.926

    01.6681.9753.3494.324

    01.7682.4353.8544.567

    01.8352.8854.4745.432

    0.0712.5463.355.1785.632

    0.2132.7683.9956.0826.125

    0.3562.8754.7537.0497.432

    0.522.9875.6268.2028.346

    0.7453.0116.4828.5549.231

    1.014.5637.6189.8489.658

    1.3594.6788.7411.313.235

    1.8024.8799.94613.0415.57

    2.3285.32611.4814.8515.687

    2.9386.43213.1516.9318.325

    3.6167.45614.941919.543

    4.5129.45316.620.8921.1

    5.45710.23418.4321.421.25

    6.53112.47520.3522.5423.54

    7.69714.57222.2623.4325.71

    8.93715.567224.125.4327.87

    10.3517.28325.9926.32530.17

    11.9218.567827.8629.4532.41

    13.5820.23529.7931.2334.6

    15.3122.47331.6833.25636.92

    17.224.83833.2335.1238.85

    18.9426.478334.5935.67840.51

    20.7628.5436.137.12542.2

    22.3632.56737.2639.2543.55

    24.2633.567238.5440.2344.79

    25.834.562739.5941.68545.82

    27.2835.67840.1542.3846.76

    28.4436.12540.7542.987447.51

    29.5136.83741.2643.78548.04

    30.2537.1241.5943.97548.53

    31.0437.98741.8844.1548.69

    31.4338.01242.0244.3248.91

    31.9138.3742.0144.4348.63

    32.138.12541.944.6148.21

    32.0537.987741.7544.2348.02

    31.6537.45241.2843.76547.72

    31.1936.89540.9142.96247.44

    30.8335.5640.6142.56346.66

    30.1934.675340.0542.1246.07

    29.5633.98739.4241.65745.38

    28.7732.85438.7440.49844.66

    27.932.36438.1640.21543.95

    26.9231.59637.6939.25843.19

    26.0231.27437.0739.142.53

    25.0630.48636.4238.4341.68

    24.0829.9835.8337.8441.1

    23.1929.12535.2437.2140.53

    22.1428.45834.5436.5439.96

    21.2528.12534.0436.239.1

    20.4127.68533.3335.4238.52

    19.6227.12533.0735.2138.2

    18.7326.98432.634.7537.62

    17.9226.34132.2934.5636.97

    17.1625.87431.8533.4536.43

    16.4724.9331.2733.2536.04

    15.8124.5130.8432.8435.69

    15.1224.0130.5232.5135.52

    14.5223.630.1532.1535.02

    14.0223.1629.8431.8434.55

    13.3722.7329.5731.5434.3

    12.8822.2329.2431.2533.96

    12.3821.8728.9730.9533.39

    11.9821.4128.530.5432.97

    11.5520.8128.1430.1232.6

    11.1420.1827.5829.56732.08

    10.7620.4127.2229.2231.74

    10.4719.6226.7928.7531.17

    9.96218.6226.6328.6330.43

    9.55917.8226.0728.0130.05

    9.35417.0925.4627.4629.57

    9.08216.4824.9726.9728.9

    8.75215.8424.3726.4728.2

    8.46815.1823.825.76527.67

    8.19914.5223.2524.78626.99

    7.88413.922.824.59826.38

    7.54113.3122.2324.37225.85

    7.17312.5621.6223.15625.12

    6.89511.8821.0722.67824.18

    6.61711.2920.5522.57323.4

    6.35810.6519.7622.12422.72

    6.0610.2319.2321.54322.02

    5.7399.82318.6221.34621.2

    5.3979.44217.8220.78420.55

    5.1779.06317.0919.56219.78

    4.8938.7916.4819.25618.9

    4.6258.5215.8418.54618.28

    4.3188.19215.1817.25917.43

    4.0647.90614.5216.12616.63

    3.8587.62813.915.32515.85

    3.5747.35713.3114.87515.19

    3.2937.19512.5614.2115.01

    3.0267.03911.8813.25814.285

    2.8676.81311.2912.45213.483

    2.6646.67310.6511.25312.235

    2.4486.42310.110.87511.857

    2.2796.1399.60210.25411.251

    2.0945.869.17310.12910.98

    1.9135.6358.6619.87410.583

    1.7175.4548.2789.25110.125

    1.615.2427.8438.9859.93

    1.4765.0437.4158.1259.231

    1.3854.7757.0427.9238.893

    1.2354.5746.6567.258.251

    1.1454.316.3026.8437.832

    1.0394.1416.0356.5827.351

    0.963.9445.7646.5216.982

    0.8663.7425.4516.2016.238

    0.7663.5245.1165.9836.018

    0.6963.3254.8095.6215.716

    0.6453.1174.5935.215.448

    0.6232.8974.4764.8295.146

    0.5482.7154.2654.6724.947

    0.482.5324.0274.3254.733

    0.4142.3673.8284.2154.429

    0.3812.2213.6214.1854.239

    0.3082.053.4783.8274.039

    0.2751.8623.3363.7213.886

    0.2511.7343.1423.6123.695

    0.2081.5992.9943.3153.467

    0.1711.4762.8473.2713.312

    0.1361.3522.7083.1853.148

    0.0921.2212.5342.853.01

    Intensity after 5 min

    Intensity after 10 min

    Intensity after 15 min

    Intensity after 20 min

    Intensity after 25 min

    Wavelength (nm)

    Fluorescent Intensity

    Fluorescent Intensity Released by Nanocapsules in the Absence of Light

    Chart3

    32.122.1

    38.3735.61

    42.0259.77

    44.6164.73

    48.9164.23

    45.3263.32

    39.1458.72

    26.8755.28

    26.2147.68

    26.0827.63

    Nanocapsules under the absence of light

    Nanocapsules energized by UV light

    Time (min)

    Fluorescent Intensity

    Fluorescent Intensity Released by Nanocapsules over Time

    Chart4

    32.122.1

    38.3735.61

    42.0259.77

    44.6164.73

    48.9120

    In the Absence of Light

    Energized by UV Light

    Time (min)

    Fluorescent Intensity

    Sheet1

    DK1UV1TimePeaks

    Data PointsData PointsDKUVDKUV

    nmDataDataDataDataDataDataDatanmDataDataDataDataDataData032.122.122.9824.12

    460000.2360.8180.9520.8530.3715.4575.1644.6854600000.8180.8010.7940.3610.3510.3710.236538.3735.6126.6929.31

    461000.2460.8281.240.9340.3995.3595.1614.4894610000.8280.8670.8120.3790.3670.3990.2461042.0259.7727.729.6

    462000.2510.81.2350.9520.3554.7494.7284.0284620000.80.8430.8250.3930.3870.3550.2511544.6164.7329.632.19

    463000.2440.8781.6431.1560.3564.0224.0453.4224630000.8780.8310.8340.4260.4150.3560.2442048.9164.2330.447.75

    464000.2690.8951.7821.2560.3553.4083.3822.8714640000.9060.8920.8660.4820.4260.3550.2692545.3263.3234.640.32

    46500.1340.3120.9691.7091.3560.3532.8632.8432.3854650000.9690.9350.9120.570.4860.3530.3123039.1458.7227.833.21

    46600.2450.3510.9951.9231.5720.3762.4882.4582.074660001.0270.9750.9430.6690.5830.3760.3516026.8755.2823.6227.67

    46700.2780.3871.1022.3521.6920.4282.3222.2241.9224670001.1021.0480.9650.7580.7230.4280.38712026.2147.6820.4524.68

    46800.3460.461.2272.631.9280.4972.2242.1151.8714680001.2271.1531.2560.8540.7930.4970.4624026.0827.6319.2319.16

    46900.480.5521.3712.8732.0510.5522.1692.0791.8944690001.3711.2251.3460.9860.8950.5520.552

    47000.9340.6561.512.8322.1560.6182.1081.9951.8444700001.511.361.8671.1591.0450.6180.656

    47101.1450.791.7142.9652.2560.7412.0511.9731.7634710001.7141.511.6731.3521.2450.7410.79

    47201.2580.9211.9462.9872.4160.8652.061.9281.7994720001.9461.7541.6921.5681.3560.8650.921

    47301.451.1232.1953.0122.7121.0432.1281.9781.8734730002.1951.9761.8761.8441.5861.0431.123

    47401.3781.3742.4683.7842.8361.2432.1532.0131.9574740002.4682.2472.1252.2391.6791.2431.374032.122.1

    47501.4561.6632.8583.9263.2361.5342.1972.0552.012475000.0782.6452.6332.5462.8582.5981.5341.663538.3735.61

    47601.6681.9753.3494.3243.5671.8212.2812.1222.12347600.1250.2113.1223.2363.1253.3493.0631.8211.9751042.0259.77

    47701.7682.4353.8544.5673.6322.1992.4762.3162.2947700.2540.3683.7513.5673.2353.8543.5612.1992.4351544.6164.73

    47801.8352.8854.4745.4323.7352.6272.6762.5222.50947800.4370.5764.4844.3264.1294.4744.0962.6272.8852048.91

    4790.0712.5463.355.1785.6324.2163.0962.9322.7392.7754790.0070.5360.7995.3285.2314.685.1784.7343.0963.5625

    4800.2132.7683.9956.0826.1254.4563.5963.2943.1043.1974800.0530.9321.116.1936.2375.436.0825.4693.5963.96

    4810.3562.8754.7537.0497.4325.2364.2863.7643.5273.6584810.1611.3461.4337.3247.2126.977.0496.4194.2864.097

    4820.522.9875.6268.2028.3465.6325.0184.2563.9844.174820.2541.8211.8358.498.3627.348.2027.4375.0184.526

    4830.7453.0116.4828.5549.2316.3255.854.7694.5414.7164830.4022.1992.3259.8189.7238.459.4458.5545.855.141

    4841.014.5637.6189.8489.6586.8926.8425.3765.1525.3934840.622.6272.95911.3510.3269.23410.839.8486.8425.728

    4851.3594.6788.7411.313.2358.3467.9886.1025.8166.1224850.8753.0963.6813.0811.23510.2312.3911.37.7626.394

    4861.8024.8799.94613.0415.579.2369.056.8126.5346.9054861.1583.5964.51115.1515.23614.2314.1813.048.6687.16

    4872.3285.32611.4814.8515.68710.23610.37.6057.3597.7624871.4984.2865.47117.416.23615.23616.2214.8511.428.014

    4882.9386.43213.1516.9318.32512.36211.818.4748.3118.6684881.9275.0186.61619.6218.23616.2318.2816.9313.258.936

    4893.6167.45614.941919.54315.23613.359.4429.2199.6284892.4175.858.0482221.2520.4320.531915.949.855

    4904.5129.45316.620.8921.115.89215.110.5110.2810.734902.9866.8429.63824.7424.53623.6522.8521.0818.35210.99

    4915.45710.23418.4321.421.2518.32616.7511.5911.3611.914913.6087.98811.4127.6126.23525.3425.4823.2519.5412.18

    4926.53112.47520.3522.5423.5421.23618.4612.8112.5913.124924.329.0513.530.5729.12628.5628.0225.8121.5313.35

    4937.69714.57222.2623.4325.7121.32620.2713.9813.7714.244935.17410.315.6933.5531.23630.6530.728.3524.3514.6

    4948.93715.567224.125.4327.8722.56