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Molecular Biology Chapter 10: DNA Replication
Worksheet I. DNA Structure
Things to keep in mind:
• how DNA, chromatin and chromosomes all fit together
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Things to keep in mind about DNA pairing:
• base pairs • 3’ and 5’ ends
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• Each strand of the double helix is oriented in the opposite direction
Figure 10.5B
5′ end 3′ end
3′ end 5′ end
P
P
P
P
P
P
P
P
3’ END: 5’ END:
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Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• Three representations of DNA
Figure 10.3D
Ribbon model Partial chemical structure Computer model
Hydrogen bond
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II. DNA REPLICATION Overview of DNA Replication – both parental strands serve as template strands:
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• Untwisting and replication of DNA
Figure 10.4B
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ORIGIN OF REPLICATION:
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings
• DNA replication begins at specific sites
10.5 DNA replication: A closer look
Figure 10.5A
Parental strandOrigin of replication
Bubble
Two daughter DNA molecules
Daughter strand
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PRIMING DNA
• know which end nucleotides are added to
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LEADING AND LAGGING STRANDS:
• know which strand is leading and which is lagging • know how the following enzymes are involved:
- helicase - single stranded binding proteins - RNA primase - DNA polymerase - DNA ligase
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REVIEW: