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MATERI 7PERKEMBANGAN KONSEPTUS
MK. Ilmu Reproduksi
1LABORATORIUM REPRODUKSI TERNAK
FAPET UB
SUB POKOK BAHASAN
• Proses pembelahan sel zygote
• Differensiasi sel embrio dan perkembangan embrio awal
• Proses implantasi
• Faktor-faktor yang mempengaruhi perjalanan embrio hingga terjadinya implantasi
2LABORATORIUM REPRODUKSI TERNAK
FAPET UB
Oviductal Transport
in theMare
Ovulation
Ovary
Oviduct
Uterine Horn
UnfertilizedOva
Blastocyst
PGE Release
Only fertilized ova are transportedinto the the uterine horn of the mare
3LABORATORIUM REPRODUKSI TERNAK
FAPET UB
LABORATORIUM REPRODUKSI TERNAK FAPET UB
4
Ovulasidan Fertilisasi
Early Embryonic Development
All cell divisionsare mitotic Smallercell size with eachcleavage division
SeparateBlastomere’sfor twins
Enters uterus -Even if not fertilizedExcept Mare- Unfertilized remainin oviduct
Fuse twomorulatogether fora chimera
Ampullary- 0-2 One cellIsthmicJunction
1-3 Two cellAmpullary-IsthmicJunction
Isthmus 2-3 Four cell
Isthmus 3-5 Eight cell
Uterus 4-5 Sixteencell
Uterus 5-8 Morula
Location Day Development
Two PolarBodies
5LABORATORIUM REPRODUKSI TERNAK
FAPET UB
LABORATORIUM REPRODUKSI TERNAK FAPET UB
6
Day Development
6-7 Tight MorulaCompact Morula formtight cell contacts so thatBlastocyst formation is possible
7-8 Early Blastocyst
7-9 Blastocyst
Fills with fluid in center -Cells have established polarity
Blastocoel
8-10 Expanded Blastocyst
Trophoblast -Forms Placenta
Inner Cell Mass -Forms Embryo
If split ICM get twins
9-11 Hatching Blastocyst
Contraction and expansion of blastocystas well as enzymes are involved withzona removal
Picture reproduced with permission of Academic Press In: Physiology and Technology of ReproductionPicture courtesy of Dr. J.E. Flechon
Unpolarized Morula Polarized Morula
Blastocyst
Blastocoel
Inner CellMass
TrophectodermBlastocoel
7LABORATORIUM REPRODUKSI TERNAK
FAPET UB
LABORATORIUM REPRODUKSI TERNAK FAPET UB
8
LABORATORIUM REPRODUKSI TERNAK FAPET UB
9
Embrio Hatching
Terjadi Implantasi
(Ternak Bunting)
LABORATORIUM REPRODUKSI TERNAK FAPET UB
10
Embryo sapi
Blastocyst - Nuclei
Inner CellMass
Photomicrograph courtesy of Dr. Daniel Pomp11
LABORATORIUM REPRODUKSI TERNAK FAPET UB
Time comparisons from ovulation during cleavage for different farm species
1 cell 8 Cell Blastocyst Enter Uterus Length ofSpecies (hours) (Days) Gestation
Cattle 24 3 8 3.5 281
Horse 24 3 6 5 337
Sheep 24 2.5 7 3 148
Swine 14-16 2 6 2 114
12LABORATORIUM REPRODUKSI TERNAK
FAPET UB
Differentiation o f Conceptus Cell Types
1
2
3Three Layers Form Day 14
Expanded Blastocyst
Inner Cell Mass
13LABORATORIUM REPRODUKSI TERNAK
FAPET UB