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LSSF Experiment: Phytobenthic LSSF Experiment: Phytobenthic ColonizationColonization
Michael D. YardMichael D. YardDean W. BlinnDean W. Blinn
US Geological SurveyUS Geological SurveyGrand Canyon Monitoring and Research CenterGrand Canyon Monitoring and Research CenterNorthern Arizona UniversityNorthern Arizona University
LSSF Experiment: Phytobenthic LSSF Experiment: Phytobenthic ColonizationColonization
Michael D. YardMichael D. YardDean W. BlinnDean W. Blinn
US Geological SurveyUS Geological SurveyGrand Canyon Monitoring and Research CenterGrand Canyon Monitoring and Research CenterNorthern Arizona UniversityNorthern Arizona University
Is colonization an immediate response upon substrate Is colonization an immediate response upon substrate submersion?submersion?
Or, is there a time-lag? And if so, is this dependent on the degree of prior cobble conditioning?
• When does this When does this newly submerged newly submerged area become area become biologically biologically productive? productive?
Study Objectives
• Determine if there were differential rates in phytobenthic colonization
• Determine most likely mode of propagation
• Determine accrual rates for algae and invertebrates
Sampling Approach
• Treatment Types– 2 Previously Colonized
• Scrapped never desiccated
• Scrapped and desiccated 1 yr
– 1 Never Colonized• (>100 yr)
• Sampling Period – Colonization period (105 d)
– 1 June 2000 to 12 September 2000
• Sampling Trips - 11
• Sampling Frequency– 10 to 12 d sampling interval
• Random block design– Randomly assigned cobbles
– Perpendicular Transects• 11 transects/treatments (3)
and control (1)
• 44 transects
• 20 cobbles/transect
• Randomly Assigned– Sample point location
• Pre-assigned – Transect
– Sample
– Samples independent
• Sampling Template • 4 cm dia.
• Rapid Assessment Procedure (Blinn et al. 1998)
• Field sorted (n = 880) – 14 Gross Categories
» Algae
» Macrophytes
» Invertebrates
– Dry Weight Determination (g)• AFSM Conversion (Shannon
et al. 2000)
• Sampling Approach
Experimental Closure (105 d)
Treatment 1, No previous colonization, exposed > 100 yrTreatment 2, Previously colonized, exposed for 1 yrTreatment 3, Scrapped not exposed to prolonged desiccationControl Cobble
ALGAL BIOMASS
0
1
2
3
4
5
6
7
1 10 20 31 41 51 61 72 82 93 106
TIME
AF
DM
- g
C m
-2
0
10
20
30
40
50
60
70
Treat #1
Treat #2
Treat # 3
• Treatment 1 & 2,– Not significantly different
– Conditioning Trend
• Response Time– 50 to 60 days before it accrued any
appreciable biomass
• Flood Effect– Not significant
– Cobble displacement• 5 %
• Mode of Propigation– Zoospores (80%
• Ulothrix/Zygnematales
• Cladophora
– FragmentationF
all S
pike
Treatment 1 & 2
ALGAL BIOMASS
0
1
2
3
4
5
6
7
1 10 20 31 41 51 61 72 82 93 106
TIME
AF
DM
- g
C m
-2
0
10
20
30
40
50
60
70
80
Treat #1
Treat #2
Treat # 3
• Treatment 3 - Mode of Propigation
– Significantly different
– Basal holdfast structure• Predominantly
Cladophora
• Ulothrix/Zygnematales
– Fragmentation
• Response Time– 10 to 20 days before it
accrued any appreciable biomass
– Increased in biomass• 50 to 60 g m-2 AFDM
– Accrual Rate• 1 g m-2 d-1 AFDM
• Asymptote– 60 days
• Flood Effect– Cobble displacement
• 5 %
Treatment 3
• Invertebrate Composition– 90 to 95% Snail biomass– Maximum biomass
• T1 5.8 g m-2 • T2 11.2 g m-2
– Densities• T1 26 x 103 m-2 • T2 35.5 x 103 m-2
– Treatments 1 & 2• Significantly different
• Response Time– Initial invert biomass
• 0.95 g m-2 (SD 0.5)
– 50 to 60 days before it accrued any appreciable biomass
– Significantly correlated to phytobenthic biomass
INVERT BIOMASS
0
2
4
6
8
10
12
14
16
18
1 10 20 31 41 51 61 72 82 93 106
TIME
AF
DM
- g
C m
-2
0
10
20
30
40
50
60
Treat #1
Treat #2
Treat # 3
• Flood Effect– Significant reduction
between days 93 & 105
Treatment 1 & 2
• Flood Effect– T3 Invertebrate biomass did not significantly
change
– Invertebrate densities did not significantly change
INVERT BIOMASS
0
2
4
6
8
10
12
14
16
18
1 10 20 31 41 51 61 72 82 93 106
TIME
AF
DM
- g
C m
-2
0
10
20
30
40
50
60
Treat #1
Treat #2
Treat # 3
Treatment 3 • Invertebrate Composition– 90% Snail biomass
• Response Time– Immediate response (10 d) – Significantly correlated to
phytobenthic biomass
• Snail biomass– Mean maximum biomass
• T3 38.1 g m-2
• Snail Densities – Mean maximum Densities
• T3 108 x 103 m-2
• Proportion of snail biomass to total biomass
– Treat 1 = 82% (SD 0.16)
– Treat 2 = 73% (SD 0.21)
– Treat 3 = 47% (SD 0.20)
Fal
l Spi
ke
0
50
100
150
200
250
300
0 20 40 60 80 100
DAYS
AF
DM
gC
m-2
Predicted Treat 1 Treat 2 Treat 3 Control
Sum of all Photosynthetic Components
0
50
100
150
200
250
300
0 20 40 60 80 100
DAYS
AF
DM
gC
m-2
Predicted Treat 1 Treat 2 Treat 3 Control
Primary Production Estimate (Assumes no loss)Fall Spike Flow
ALGAL BIOMASS
0
50
100
150
200
250
300
0 20 40 60 80 100
DAYS
AF
SM
(g
m2 )
Treatment 3
CONTROLCladophora 30 to 75% photosynthetic biomass
ALGAL/SNAIL BIOMASS
0
50
100
150
200
250
300
0 20 40 60 80 100
DAYS
AF
SM
(g
m2 ) Snail biomass to high to be supported by algal biomass
• Attained >90 gC m-2; Densities >200 x 103 m-2
• Algal Reduction by Snails (density dependent)
– Hunter 1980
– Mulholland et al. 1983
– Jacoby 1985
– Steinman et al. 1987
– Lowe and Hunter 1988
– Osenberg 1989
– Underwood and Thomas 1990
– Bronmark et al. 1991
– Tuchman and Stevenson 1991
– Hill et al. 1991
– Steinman 1992
– Rosemond et al. 1993
• Algal Biomass increase in response to grazing
– Removal of senescent cells
• Lamberti and Resh 1983
• Swamikannu and Hoagland 1989
– Nutrient cycling
• McCormick and Stevenson 1991
• Stewart 1987
• Mulholland et al. 1991
– Removal of epiphytes
• Dudley 1992
• Sarnelle et al. 1993
ALGAL BIOMASS
0
50
100
150
200
250
300
0 20 40 60 80 100
DAYS
AF
SM
(g
m2 )
Treatment 3
2.1 g/d
ALGAL BIOMASS
0
50
100
150
200
250
300
0 20 40 60 80 100
DAYS
AF
SM
(g
m2 )
Treatment 3
0.6 g /m2 d
2.1 g/m2 d
0
150
300
450
600
750
900
1050
1200
0 20 40 60 80 100
DAYS
AF
DM
gC
m-2
Net Primary Production Model Output, assumes that all net excess production is diverted toward biomass accrual (no loss in photosynthates, drift, grazing)
Asymptote due to balance between net photosynthesis and respiration demands
So, under this model net primary production So, under this model net primary production is self-limiting and constrained by size is self-limiting and constrained by size
0
150
300
450
600
750
900
1050
1200
0 20 40 60 80 100
DAYS
AF
DM
gC
m-2
8.5 gC/m2 dDOC
Consumed by Inverts/Fish
Drift
Structure
Cumulative production through time
4 gC/m2 d
?? gC/m2 d
0.25 gC/m2 d
Conclusion
• Damaged or mechanically removed thallus structure will rapidly recover (Assuming ideal growing conditions)
– If basal holdfast structure remains intact and viable
• Newly submerged substrate has a slower colonization response
– No difference in response for cobbles exposed 1 yr or greater
• Newly submerged substrate has a slower colonization response
– Colonization appears to be predominantly by zoospores
– Fragmentation does not appear to the major propagation mode
– Recovery response may be more rapid if substrate is conditioned
• Microflora (organic material, bacteria, diatom assemblage)
• We cannot be sure if colonization response would be different if grazing pressure was absent
• Stable flows may result in substantial amounts of production
– Both primary and secondary
– However, this may not be apparent by just measuring biomass
– It may be difficult to separate out effects from flows
AcknowledgementsAcknowledgements
• Northern Arizona UniversityNorthern Arizona University– Aquatic Ecology LabAquatic Ecology Lab
• Allen Haden, Ally Martinez, Molly McCormick, Ian McKinnon, Joe Shannon Allen Haden, Ally Martinez, Molly McCormick, Ian McKinnon, Joe Shannon – Geology DepartmentGeology Department
• Matt Kaplinsky, & Mark ManoneMatt Kaplinsky, & Mark Manone– FacultyFaculty
• Michael Kearsley, George Koch, Peter Price, & Rod ParnellMichael Kearsley, George Koch, Peter Price, & Rod Parnell
• Grand Canyon Monitoring and Research CenterGrand Canyon Monitoring and Research Center• Dave Baker, Carol Fritzinger, Barry Gold, Susan Hueftle, Barbara Ralston, & Dave Baker, Carol Fritzinger, Barry Gold, Susan Hueftle, Barbara Ralston, &
Jake Tiegs Jake Tiegs
• Indispensable InsistenceIndispensable Insistence• Helen YardHelen Yard