Happy Halloween!!Happy Halloween!!

1. Please take out your Cell 1. Please take out your Cell Types & Organelle Notes Types & Organelle Notes

from the last two classes from the last two classes 2. Please take out 2 blank 2. Please take out 2 blank

pieces of paper (one for pieces of paper (one for your Bellwork & one for your Bellwork & one for the Cheek & Onion Cell the Cheek & Onion Cell

Lab)Lab)

Cheek/Onion Cell Bellwork Cheek/Onion Cell Bellwork

1. 1. Complete the Venn DiagramComplete the Venn Diagram by by suggesting ways (shape/organelles) in suggesting ways (shape/organelles) in which a cheek cell which a cheek cell (animal cell)(animal cell) may be may be similar and/or differentsimilar and/or different from an onion cell from an onion cell (plant cell)(plant cell)

ONION CELLSCheek Cells

Cell Organelle Lab Cell Organelle Lab with Onion Cells and with Onion Cells and

Cheek CellsCheek Cells

Purpose of this lab:Purpose of this lab:1. To Observe Live Cells1. To Observe Live Cells

2. To Identify the Structural 2. To Identify the Structural Differences between Plant & Differences between Plant &

Animal CellsAnimal Cells3. To Identify a Cell Nucleus3. To Identify a Cell Nucleus

Lab Materials and Lab Materials and ProceduresProceduresMATERIALSMATERIALS

ToothpicksToothpicks Pieces of onionPieces of onion MicroscopeMicroscope Glass slidesGlass slides Colored PencilsColored Pencils CoverslipsCoverslips MarkersMarkers Iodine SolutionIodine Solution Methylene Blue SolutionMethylene Blue Solution Paper towelsPaper towels

PROCEDURESPROCEDURES PART I – ONION CELLPART I – ONION CELL1. Peel a THIN piece of onion 1. Peel a THIN piece of onion (the skin)(the skin)

and place in the center of the glass and place in the center of the glass slide. slide.

2. Add 2. Add 1 or 2 drops of IODINE1 or 2 drops of IODINE to the to the onion peel and place cover slip on toponion peel and place cover slip on top

3. Examine the onion slide 3. Examine the onion slide UNDER LOW UNDER LOW POWER (Medium size lens);POWER (Medium size lens); Draw what Draw what the cell looks like AND label ALL the cell looks like AND label ALL organelles you can identifyorganelles you can identify

4. Examine onion cell under 4. Examine onion cell under HIGH HIGH PowerPower; Draw what the cell looks like ; Draw what the cell looks like AND AND label ALLlabel ALL organelles you can organelles you can identifyidentify

Part 2: Examining Cheek CellsPart 2: Examining Cheek Cells

1. Choose 1 person in your group to 1. Choose 1 person in your group to scrape the inside of their cheek scrape the inside of their cheek using a flat toothpickusing a flat toothpick

2. Rub toothpick over the slide and 2. Rub toothpick over the slide and PLACE 1 drop of PLACE 1 drop of METHYLENE BLUE METHYLENE BLUE SOLUTION SOLUTION over your cheek cells over your cheek cells and cover with cover slipand cover with cover slip

3. Follow steps 3 & 4 as listed from 3. Follow steps 3 & 4 as listed from the onion lab for the drawing the the onion lab for the drawing the cheek cell. cheek cell.

Today’s SpecimensToday’s Specimens

Onions CELLS

CHEEK CELLS

RECORDING LAB DATARECORDING LAB DATADraw & Label Cheek/Onion Cell. Be sure you include ALL Cell Draw & Label Cheek/Onion Cell. Be sure you include ALL Cell

Organelles that you can see!Organelles that you can see!

Cheek CellCheek Cell Onion CellOnion Cell

Cheek Cell: Low PowerCheek Cell: Low Power Onion Cell: Low PowerOnion Cell: Low Power

Cheek Cell: High PowerCheek Cell: High Power Onion Cell: High PowerOnion Cell: High Power

Onion/Cheek Cell Post-lab Reflection QsOnion/Cheek Cell Post-lab Reflection QsCopy & AnswerCopy & Answer

1. Discuss 1. Discuss 3 observations3 observations you made of the you made of the onion cells and onion cells and 3 observations3 observations you made you made of the cheek cells. (of the cheek cells. (Be descriptiveBe descriptive))

2. How did the cheek/onion cells differ? 2. How did the cheek/onion cells differ? How were the cheek/onion cells similar?How were the cheek/onion cells similar?

3. Specifically, how did the shape of the 3. Specifically, how did the shape of the onion cell differ from the shape of the onion cell differ from the shape of the cheek cell?cheek cell?

4. Describe all of the organelles you were 4. Describe all of the organelles you were able to recognize in the cheek and onion able to recognize in the cheek and onion cell (Label clearly).cell (Label clearly).

5. Review the 3 purposes of this lab. 5. Review the 3 purposes of this lab. Explain whether EACH purpose was met Explain whether EACH purpose was met and HOW each purpose was met. and HOW each purpose was met.

Safety TipsSafety Tips THE IODINE THE IODINE

STAINSSTAINS SKIN AND SKIN AND CLOTHING SO CLOTHING SO PLEASE USE PLEASE USE CAUTIONCAUTION WHEN WHEN PLACING ON GLASS PLACING ON GLASS SLIDESLIDE

PLEASE be careful PLEASE be careful with the glass with the glass slides~ they are slides~ they are fragilefragile

Some Useful Tips…..Some Useful Tips….. Don’t BE AFRAID TO Don’t BE AFRAID TO

MAKE MISTAKES!!! MAKE MISTAKES!!! Trial and Error is the Trial and Error is the best way to learnbest way to learn

Some of the greatest Some of the greatest INVENTIONS/PRODUCTINVENTIONS/PRODUCTS of our time were S of our time were discovered by discovered by mistakesmistakes

ESPECIALLY with the ESPECIALLY with the CHEEK Cells try it a CHEEK Cells try it a few times until you few times until you can identify clearly can identify clearly visible cellsvisible cells

Materials to GatherMaterials to GatherPlease make sure your lab table has:Please make sure your lab table has:

A CLEAN paper towelA CLEAN paper towel 2 clean glass slides2 clean glass slides 2 cover slips2 cover slips 2 clean toothpicks2 clean toothpicks Working MicroscopeWorking Microscope

WHAT I EXPECT FROM YOU WHAT I EXPECT FROM YOU DURING THE LAB…..DURING THE LAB…..

1. Draw the 1. Draw the ONION and CHEEK CellONION and CHEEK Cell Using 2 Using 2 POWERS: POWERS: Low (Medium Lens) and HighLow (Medium Lens) and High on on your chart using Colored Pencils/Markers – IT your chart using Colored Pencils/Markers – IT MUST BE COLORED!!!MUST BE COLORED!!!

2. When 2. When finished clean up ALL of your finished clean up ALL of your MATERIALS, MATERIALS, complete your sketching, coloring complete your sketching, coloring and labeling diagramsand labeling diagrams

3. Answer ALL Post Lab Questions3. Answer ALL Post Lab Questions

What Am I Turning IN???What Am I Turning IN???

THIS CLASS:THIS CLASS: Onion/Cheek Cell Bellwork Onion/Cheek Cell Bellwork Onion/Cheek Lab Onion/Cheek Lab Post-Lab QuestionsPost-Lab Questions

Extra Credit Article QuestionsExtra Credit Article Questions**How much extra credit you receive **How much extra credit you receive

depends on how much effort you put independs on how much effort you put in

Write the title of the articleWrite the title of the article Write a Write a 2 paragraph2 paragraph summary of the article summary of the article What is your reaction to the article? (What is your reaction to the article? (1 paragraph1 paragraph

MINIMUM)MINIMUM) Why was this article beneficial for you to read?Why was this article beneficial for you to read? How does this article relate with biology or a unit How does this article relate with biology or a unit

we will be covering during our biology course?we will be covering during our biology course?