PowerPoint Presentation
TRNG H CNG NGHIP THC PHM TP HCMKHOA CNG NGH THC PHMng dng cng
ngh sinh hc trong cng ngh thc phm
ti: CNG NGH SX BT NGTGVHD: Nguyn Th Thu sangNhm 5:Nguyn Th Diu
Hin 2005120248Nguyn Ngc Kha 2005120260Trn L Thi Ng2005120335
NI DUNG
010203TNG QUANCNG NGH SN XUTSN PHM
Gii thiu v bt ngt, acid glutamic
2134Mt trong hn 20 loi axit amin kin to nn protein c th im nng
chy:199 CCng thc:C5H9NOKhi lng phn t:147,13g/mol
ng vai r rt quan trng trong vic trao i cht ca c th ng vtTham gia
phn ng thi loi amoniac, mt cht c vi h thn kinhAcid glutamic
S lc lch s pht trin ca Bt Ngt
Lch s pht trin ca Bt Ngt
Rittenhausen 1880190819091933IkedaAjnomoto ra isx bt ngt ti
nht
Cc phng php sn xut bt ngtPp tng hp ha hcPp ln men Pp thy phn
protitPp kt hp 01020304
Tinh bt sn
Tinh bt sn c sn xut trong qu trnh ch bin sn cC hai loi sn: sn ng
v sn ngt
Tinh bt snThnh phn ha hcHm lngTinh bt83-88%Nc10.6-14.4%Cht
khong0.1-0.6%m 0.1-0.4%Cht ha tan0.1-1.3%
R ng maR ng ma l thnh phn cn li ca dung dch ngsau khi tch phn ng
knh kt tinhThnh phn chnh ca r ng l: ng 62%, cc cht phi ng 10%, nc
20%.ng trong r ng bao gm 25%- 40% sacaroza, 15%- 25% ng kh (glucoza
v fructoza), 3%- 5% ng khng ln men c.
GING VI SINH VT TRONG SN XUT BT NGT
Micrococus Glutamicus.Corynebacterium Glutanicum
Tham gia vo qu trnh ln men sn xut axit glutamic, chng vi sinh
thng s dng l: Corynebacterium Glutanicum, Brevibacterium
Lactofermentus, Micrococus Glutamicus...Nhng ch yu nht vn l chng
Corynebacterium Glutamicum
Tinh btNcThy phnTrung haThan hot tnhp lcBng haCho vo mi trng ln
menNguyn liu c sn ngThanh trngB sung cht dd
Ln menChng VSVNhn gingTrao i IonTch acid GlutamicNc chmDch thiNc
nng & NaOHAcid ha acid GlutamicLm lnh kt tinhTrung haNc lnh
ngui 30-350C
Tip mm tinh thNui mmLy tmSySngBao giSn phmNc ciC c
Cn c vo dy truyn sn xut ta c th chia ra bn cng on nh sau
01030204Cng on thy phn tinh btCng on ln menCng on trao i ion tch
axit glutamic ra khi dch ln menCng on trung ha, tinh ch to glutamic
natri tinh khit
Cng on thy phn tinh bt
Mc ch ca cng on ny l to iu kin thc hin cc phn ng thy phn tinh bt
thnh ng ln men c, ch yu l ng glucoza Phn ng sy ra nh sau :
(C6H10O5)n nH20 n C6H12O6
Ging vsvTo mi trngBo qun gingThun ha gingLn men cp ILn men cp
IIX l Ur v du ph btX l khng khLn men cp IIIDch c ln men
X l urUr tham gia vo thnh phn mi trng gm ur u v ur tip cho qu
trnh: Ur u l ur cho vo mi trng, sau khi mi trng c thanh trng v lm
ngui nhit 320 C.Ur tip l ur b sung trong qu trnh ln men, s lng khng
c nh, khi pH dch ln men ang t trn 7 xung dn th phi tip dn ur cho t
ln 7.5-8 sau o lng axit glutamic to ra trong mi trng cng nhiu, pH
cng gim xung 7 hoc di 7 li tip ur na cho n khi ng trong dch ln men
cn khong 1% th khng cn tip na.
X l du
X l khng kh
2
Giai on gia
1
3
Giai on cui1
Giai on uLn men III
Giai on u 8-12 gi gi l giai on sinh khi , lm cho vi khun ln ln,
t kch tht cc i v bt u sinh sn, phn chia.Nhng biu hin ca giai on ny
l :Cng v cui giai on tc tng nhit cng nhanh, PH tng dn t 6.5-6.7 ln
7.5-8.Bt to thnh tng dn. Lng ng to thnh tng dn, thng 2-3 gi u hao
rt t, cng v sau tc cng nhanh.Lng t bo vi khun tng dn t khong
0,13-0,14 n s 1 (s o OD trn my so mu )Hm lng axit glutamic cha c
hoc rt t.
Giai on giaT gi th 10,12 n gi th 24,26.Giai on ny gi cho s t bo
khng tng thm na hoc tng rt t. Lng axit glutamic to thnh li ha tan
vo cc mi trng lm cho pH mi trng gim dn, CO2 bay ra nhiu, bt tng o
t.
Giai on cui Nhng gi cn li tt c cc biu hin u gim dn cho n khi hm
lng ng ch cn sau t nht 48 gi th qu trnh kt tinh kt thc . Lc ny
trong hn hp c 2 pha:Pha rn: gm axit glutamic kt tinh v lng xung.Pha
lng : gm nc v mt t axit glutamic khng kt tinh ho tan v ta gi l nc
ci Phn nc ci em i trao i li, phn kt tinh a ly tm ta c axit glutamic
m.
C c
Thit b c c mng
Thit b c c chn khng
Ly tm
Thit b ly tm
Sy bt ngtBt ngt sau khi c ly tm c ti ra khay v a i sy . B dy lp
bt ngt trong khay l 2 -> 3 cm, nhit khng kh sy t
