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Fungi opportunistic, and pathogenic

Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

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Page 1: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Fungiopportunistic, and pathogenic

Page 2: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Acremonium (Cephalosporium) sp. Delicate, septate hyphae. Conidia are oblong and usually one celled but occasionally two celled (Cotton

blue stain).

Page 3: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Acremonium (Cephalosporium) sp. Delicate, septate hyphae. Conidia are oblong and

usually one celled (Unstained).

Page 4: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Acremonium sp. Conidia (spores) of Acremonium sp. are oblong,

slightly variable in size, and usually one celled (Cotton blue stain).

Page 5: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Alternaria sp. Commonly considered a contaminant, producing large

dark conidia with septations.

Page 6: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Arthrinium sp. Dark brown lenticular conidia (Unstained).

Page 7: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Aureobasidium pullulans Dark brown conidia and hyaline hyphae (Unstained).

Page 8: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Blastomyces dermatitidis Budding yeast with broadly inserted base in pus from

spondylitis (Unstained).

Page 9: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Chaetomium sp. Perithecia with filamentous appendages in culture

(Unstained).

Page 10: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Coccidioides immitis A round spherule containing endospores in a lung

biopsy (Grocott's silver stain).

Page 11: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Coccidioides immitis Arthroconidia in a culture (Unstained).

Page 12: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Exophiala dermatitidis Abundant yeast cells and mycelium (Unstained).

Page 13: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Exophiala dermatitidis Colonies with brown pigment on Sabouraud agar.

Page 14: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Fusarium solani Large sickle-shaped, multiseptate macroconidia

(Cotton blue stain).

Page 15: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Fusarium species Large sickle-shaped, multiseptate macroconidia

(Unstained).

Page 16: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Fusarium species Mycelium with large sickle-shaped macroconidia

(Gram stain).

Page 17: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Hormographiella aspergillata Mycelium and arthroconidia from a culture on

Sabouraud’s agar (Unstained).

Page 18: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Hormographiella aspergillata Septated hyphae in lung tissue taken at autopsy

(Haemotoxylin and eosin stain).

Page 19: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Mucor sp. Wide, practically non-septate hyphae bearing terminal

round spore-filled sporangia. Here most spores have escaped (Cotton blue stain).

Page 20: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Penicillium marneffei The conidiophores, with their attached chains of

round conidia, have a brush-like appearance (Unstained).

Page 21: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Penicillium marneffei Culture on Sabouraud-agar showing diffusing red

pigment.

Page 22: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Penicillium sp. The conidiophores, with their attached chains of

round conidia, have a brush-like appearance (Cotton blue stain).

Page 23: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Pneumocystis jirovecii In bronchial aspirate. The wall of the parasite stains

dark (Silver stain).

Page 24: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Pneumocystis jirovecii In bronchial aspirate. The wall of the parasite does not

stain. It contains eight nuclei (Giemsa stain).

Page 25: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Pneumocystis jirovecii In bronchial aspirate. The cyst walls are clearly visible.

No budding (Direct immunofluorescence).

Page 26: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Pseudoallescheria boydii Septate hyphae bearing unicellular, oval conidia with

the larger end toward the apex (Unstained).

Page 27: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Pseudoallescheria boydii (Anamorph: Scedosporium apiospermum). Oval terminal conidia with the larger end toward the apex

(Unstained).

Page 28: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Scedosporium prolificans Inflated conidiogenous cells with dark, oval, and

terminal conidia (Unstained).

Page 29: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Scedosporium prolificans Colonies with aerial olivaceous mycelium on

Sabouraud agar.

Page 30: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Scopulariopsis sp. The conidia are 4 to 9 μm in diameter, often thick

walled, round, usually very rough and spiny when mature (Unstained).

Page 31: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Scopulariopsis sp. Septate hyphae. The conidia are 4 to 9 μm in diameter,

round, and spiny when mature (Unstained).

Page 32: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Scopulariopsis brevicaulis The conidia in chains are 4 to 9 μm in diameter, round,

and spiny when mature (Unstained).

Page 33: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Sporothrix schenckii Thin, septate, and branching hyphae. Small pear-

shaped or almost round conidia (Cotton blue stain).

Page 34: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Sporothrix schenckii Thin, septate, and branching hyphae. Small pear-

shaped or almost round conidia (Cotton blue stain).

Page 35: Fungi.pptm [Lecture seule]...Microsoft PowerPoint - Fungi.pptm [Lecture seule] Author dcarlier Created Date 5/11/2012 2:58:46 PM

Stachybotrys sp. Thin and septate hyphae. Dark oval single celled

conidia (Cotton blue stain).