DNA from any Sample - Deutsche Messe AG · DNA from any Sample ... are suitable for extracting high molecular weight DNA from a wide variety of sample types including tissue, fresh

Quick-DNA™

DNA from any Sample

Tel: +49 (0)761 60068710 www.zymoresearch.de

Quick-DNA™ Overview

Learn more at www.zymoresearch.de/dna/quick-dna

Zymo Research offers a range of genomic DNA isolation kits that are suitable for extracting high molecular weight DNA from a wide variety of sample types including tissue, fresh and paraffi n-embedded tissue sections, cultured cells, saliva, buccal cells, whole blood, plasma, serum, urine, bacteria, fungi, yeast, algae, viruses, and mitochondria. Our genomic DNA isolation kits yield high-quality DNA that is ideal for use in any sensitive downstream applications such as PCR, DNA sequencing, endonuclease digestion, and methylation detection.

Biological Fluids,Cells & Tissues

Quick-DNA™ Plus Kits

Liquid Biopsy Serum, Plasma,

Urine, etc.

Quick-cfDNA™ Serum & Plasma Kit

Quick-DNA™

Urine Kit

FFPE Samples Quick-DNA™ FFPE Kit

Cultures

Environmental(Fecal, soil, water fi ltrate and other)

Plants

Quick-DNA™ Fungal/Bacterial DNA Kits

MicrobialQuick-DNA™ Fecal/Soil

Microbe DNA Kits

For DNA isolation in microbiomic studies, see our ZymoBIOMICS™

product line.

Quick-DNA™ Plant/Seed Kits

www.zymoresearch.deTel: +49 (0)761 60068710

Quick-DNA™ Plus KitsHighlights

• Extract high-quality DNA easily and reliably from any biological fl uids, cultured/monolayer cells, or solid tissues

• Zymo-Spin™ technology ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.

vs.

Supplier Q

2-3 μl Contamination

Zymo Research0 μl

Carryover

Zymo-Spin™ technology ensures no carryover of buffer, salts, or other PCR inhibitors. DNA is ready for all sensitive downstream applications such as qPCR, Next-Generation sequencing arrays, and methylation analysis.

DNA Yields Increase Linearly with Increasing Volumes of Human Whole Blood Using the Quick-DNA™ Miniprep Plus Kit. Six replicates of 25, 50, 100, and 200 µl of human whole blood were processed.

Human Whole Blood (µl)

R2=0.9936

8.00

7.00

6.00

5.00

4.00

3.00

2.00

1.00

0.00

0 25050 200100 150

Tota

l Yie

ld (µ

g)

High-quality DNA obtained from a wide range of biological samples using the Quick-DNA™ Miniprep Plus Kit. DNA purifi ed using the Quick-DNA™ Miniprep Plus Kit is ultra-pure, highly concentrated, and ready for all downstream applications. Input DNA was standardized to 300 ng and analyzed in a 1% (w/v) TAE/agarose/EtBr gel. The size marker “M” is a 1 kb ladder (Zymo Research).

HumanBlood

PorcineBlood

HeLaCells

BuccalSwab

HumanSaliva

MouseTail

MouseKidney

MouseBrain

BovineMuscle

BovineMilk M

Product Cat. No. SizeQuick-DNA™ Microprep Plus Kit D4074 50 preps.

Quick-DNA™ Miniprep Plus Kit

D4068T 10 preps.

D4068 50 preps.

D4069 200 preps.

Quick-DNA™ Miniprep Kit(no Proteinase K)

D3024 50 preps.

D3025 200 preps.

Learn more and view additional Quick-DNA™ Kit formats at www.zymoresearch.de/dna/quick-dna

Purity Redefi ned Reliable & Consistent

High-quality DNA from any sample


Tel: (949) 679-1190 www.zymoresearch.com

Quick-cfDNA™ Serum & Plasma KitHighlights

• High-quality DNA, including cell-free, is easily and robustly purifi ed from up to 10 ml of serum/plasma or up to 1 ml amniotic fl uid or cerebrospinal fl uid.

• Zymo-Spin™ Column technology enables elution of DNA in as little as 35 µl and ensures it is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing.

Product Cat. No. Size

Quick-cfDNA™ Serum & Plasma Kit D4076 50 preps.

Total DNA is effi ciently purifi ed from cell-free biological fl uids with the Quick-cfDNA™ Serum & Plasma kit. Total DNA, including both high and low molecular weight species, purifi ed (duplicates) from human maternal plasma, amniotic fl uid and cerebrospinal fl uid was analyzed by 2200 TapeStation® (Agilent).

The Quick-cfDNA™ Serum & Plasma kit robustly recovers cell-free DNA when compared to Supplier Q. The DNA recovered is linearly proportional to the input volume of sample when compared with Supplier Q. Concentration of the smallest nucleosomal fragment (N1, ~180bp) DNA was determined using the 2100 Bioanalyzer (Agilent).

Ladder healthy serum Maternal plasma Amnio4c fluid

Cerebrospinal Fluid (CSF)

48,500bp 15,000bp 7,000bp 4,000bp 4,000bp 3,000bp 2,500bp 2,000bp 1,500bp 1,200bp 900bp 600bp 400bp 250bp 100bp

Fragmented DN

A

Total DNA

A) Maternal plasma

Amniotic fl uid

Cerebrospinal fl uid La

dder healthy serum Maternal plasma Amnio4c fluid Cerebrospinal Fluid (CSF)

48,500bp 15,000bp 7,000bp 4,000bp 4,000bp 3,000bp 2,500bp 2,000bp 1,500bp 1,200bp 900bp 600bp 400bp 250bp 100bp

Fragmented DN

A

Total DNA

A) 800

700

600

500

400

300

200

100

0

0 1 2 3 4 5

Cel

l fre

e D

NA

yie

ld (p

g/µ

l)

Plasma Volume (mL)

ZymoSupplier Q

Cell-free DNA recovery is directly scalable using the Quick-cfDNA™ Serum & Plasma Kit. Graphs and gel image show the linear recovery of cfDNA from human plasma and serum (healthy female donors), as measured by 2200 Tapestation® (Agilent, in duplicates).

Plasma/Serum

cfD

NA

[ng

/µl]

2

01 5 10 ml

R2=0.99 (36yr F serum)

R2=0.97 (58yr F serum)

R2=0.99 (54yr F plasma)



36 yr F plasma

[bp]

Reliable & Effi cient Recovery of Cell-free DNA (up to 10 ml)

Compatible with Many Body Fluids Superior Performance



Tel: (949) 679-1190 www.zymoresearch.com

Quick-DNA™ Urine KitHighlights

• Purify cellular and/or cell-free DNA easily and reliably from up to 40 ml of urine.

• Uniquely formulated urine conditioning reagent allows stabilization of DNA in urine for up to one month at ambient temperature.

• Zymo-Spin™ Column technology ensures DNA is ready for all sensitive downstream applications including qPCR, DNA-sequencing, arrays, and DNA methylation analysis.


Quick-DNA™ Urine Kit D3061 50 preps.

DNA yields increase linearly with increasing volumes of urine from healthy subjects extracted using the Quick-DNA™ Urine Kit. DNA was isolated from 1 ml, 10 ml, 25 ml, and 40 ml urine. DNA concentration was quantifi ed using the Femto™ Human DNA Quantifi cation Kit (Zymo Research, E2005).

900

800

700

600

500

400

300

200

100

0

0 10 20 30 40

DN

A (n

g)

Urine (ml)

Total (Cellular and Cell-free)

Cellular only

Cell-free only

Both cellular and cell-free DNA can be effectively purifi ed from urine using the Quick-DNA™ Urine Kit. 5 ml of urine from a healthy female donor was processed and DNA was eluted in 20 µl fi nal volume. 1 µl of the sample was analyzed using a 2200 TapeStation.

[bp]48,50015,000

7,000

4,000

3,0002,5002,000

1,5001,200

900

600

400

250

100

Cellular DNA

Urine Total DNALadder

Cell-free DNA

Get cellular or cell-free DNA, or both!

Streamlined, Innovative Workfl ow


Precipitation Proteinase K Digestion DNA Purifi cation

BindWashElute

Spin

DNA


Highlights

• Streamlined purifi cation of high-quality FFPE tissue DNA that is ideal for PCR, Next-Generation library prep, enzymatic manipulations, etc.

• Size selection technology; recover total DNA >50 bp or >500 bp.

Quick-DNA™ FFPE Kit

$

$

Deparaffi nized Tissue

$Proteinase K Digestion

Ultra-pure DNA Ready for PCR, Sequencing, etc.

SpinWashElute

Zymo-Spin™ IIC


Quick-DNA™ FFPE Kit D3067 50 preps.

100bp DNA >500bp 1kb DNA >50 bp

Equivalent amounts of DNA resolved in a 1% agarose/TAE/EtBr gel show binding conditions may be adjusted with the Quick-DNA™ FFPE Kit to selectively isolate DNA >50 bp or >500 bp. 100 bp DNA ladder and 1 kb DNA ladder from Zymo Research.

500 bp ◄

Selectable DNA Size

Improved Recovery from FFPE Samples

Streamlined Workfl ow


Flourescence History

Flou

resc

ence

(465

-510

)

Cycles

Zymo

Supplier Q

1 2 25 28 31 34 37 40 43

Equivalent amounts of DNA isolated using Zymo and Supplier Q procedures were used for real time PCR analysis. DNA isolated using the Quick-DNA™ FFPE Kit consistently yielded lower Ct values values as depicted by the amplifi cation curves above.


Quick-DNA™ Environmental Kits


Quick-DNA™ Fecal/Soil Microbe Miniprep Kit D6010 50 preps.

Quick-DNA™ Fungal/Bacterial Miniprep Kit D6005 50 preps.

Quick-DNA™ Tissue & Insect Miniprep Kit D6016 50 preps.

Quick-DNA™ Plant/Seed Miniprep Kit D6020 50 preps.

Improved Recovery of High-quality DNA from Environmental Samples

Ultra-pure, Inhibitor-free DNA


Our environmental purifi cation kits feature unique BashingBead™ technology, allowing high-recovery of DNA from a wide variety of tough-to-lyse environmental samples. These products lead to high-yield and quality DNA suitable for sensitive downstream applications.

Our novel inhibitor removal technology, featured in our fecal/soil microbe and plant/seed kits, contains the components needed for effi cient removal of polyphenolic compounds that can inhibit downstream enzymatic reactions.

Comparison of DNA yields from rat feces using the Quick-DNA™ Fecal/Soil Microbe Kit and kits from suppliers A and B. Equivalent amounts of feces were processed using each kit and then equal volumes of eluted DNA were analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. Samples were processed in triplicate.

Supplier A Supplier BQuick-DNA™ Fecal/

Soil Microbe Kit

Fungal and bacterial DNA purifi cation. DNA isolated from Saccharomyces cerevisiae (spores) and E. coli using the Quick-DNA™ Fungal/Bacterial Kit is high-quality and structurally intact. Equivalent amounts of yeast and bacteria were processed using the Quick-DNA™ Fungal/Bacterial Kit or the kit from Supplier A. Equal volumes of eluted DNA were then analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The size marker is a 1 kb ladder (Zymo Research).

1 kb Yeast Spores E. coli

Zymo Research Supplier A

Yeast Spores E. coli

Quick-DNA™ Fungal/Bacterial Kit

No Am

plification

No Am

plification

No Am

plificationN

o Amplification

C T

10% Eluate

35% Eluate

ZymoBIOMICS Supplier M Supplier P Supplier Q

17.57 17.48 17.63 17.82

0.00

5.00

10.00

15.00

20.00

25.00

30.00

35.00

40.00

The Quick-DNA™ Environmental Kits provide inhibitor-free DNA even when challenged with extremely inhibitor-rich samples.

Quick-DNA™


Innovation. Pure & Simple.™

Since its inception in 1994, Zymo Research has been proudly serving the scientific community by providing innovative, reliable, and high quality research tools at affordable prices. Our vision... “The Beauty of Science is to Make Things Simple” is now truer than ever. Whether it’s epigenetics, DNA, RNA, E. coli, or yeast based research, our philosophy remains the same: To provide the highest quality products in the industry while ensuring they are both simple to use and reliable in their performance.

Zymo Research stands on three pillars which form the foundation of our company: Innovation, Quality, and Customer Service. These pillars are fundamental to our culture and ensure our products meet your needs.

InnovationZymo Research is historically recognized for its innovation of high quality nucleic acid purification technologies. Under the branding DNA Purification Made Simple® and RNA Purification Made Simple®, our technologies are pushing the limits of what is possible with nucleic acid isolation. As The Epigenetics Company™, Zymo Research has also received much attention for its rapidly expanding portfolio of epigenetics products and services. It is our objective to develop and provide the most comprehensive set of tools for DNA, RNA, and epigenetic research and analysis available today. Thousands of peer-reviewed scientific publications from researchers around the world feature our technologies. Through innovation, our scientists have made streamlined DNA methylation detection possible, pioneered the micro-elution column for DNA and RNA purification, developed the simplest and the most sophisticated methods for high-quality plasmid DNA purification, and patented the first RNA purification directly from Trizol® without phase separation among many other leading technologies in the industry.

QualityWe are committed to quality and guarantee that all of our products and service will meet and exceed your expectations. Our products are constantly evaluated by scientists like you to help ensure their reliability and the highest standard of quality.

Customer ServiceWe strive for excellence in how we support your scientific endeavors. We pledge to be honest and responsible for everything we do with you. We will treat you as we would like to be treated. Together, we will build a brighter future.

Tel: +49 (0)761 60068710 www.zymoresearch.com

Cat. No. B1001 Version 01

Documents

DNA from any Sample - Deutsche Messe AG · DNA from any Sample ... are suitable for extracting high molecular weight DNA from a wide variety of sample types including tissue, fresh