RNA from Any Sample - donar.messe.dedonar.messe.de/.../2017/F753061/rna-from-any-sample-eng-519881.pdfRNA from Any Sample Tel: ... High levels of genomic DNA contamination are present

RNA from Any Sample

Tel: +49 (0)761 60068710 www.zymoresearch.de

RNA Isolation OverviewZymo Research offers an assortment of products that allow for the simple, rapid, and effi cient isolation of total RNA from a variety of biological sources including fresh, frozen, or paraffi n-embedded tissues, cultured cells, buccal cells, whole blood, plasma, serum, urine, yeast, or RNA viruses. All of our RNA isolation kits feature Zymo-Spin™ Column technology, which yields highly concentrated RNA perfect for applications such as microarrays, denaturing-gel electrophoresis, Northern blotting, RT-PCR, and all sensitive downstream applications. Each kit has been optimized for a particular application with specialized, nuclease-free components to ensure: 1) Maximum levels of membrane solubilization and cellular disruption, 2) Total inhibition of nuclease activity, 3) Complete deproteinization of the sample, 4) Effi cient isolation and concentration of the RNA, 5) Stabilization and safe storage of the RNA.

Culture

Environmental(Fecal, soil, water fi ltrate and other)

Plant

Quick-RNA™ Fungal/Bacterial Kits

Quick-RNA™ Fecal/Soil Microbe Kit

Quick-RNA™ PlantMiniprep Kit

Fixed Tissues

Microbial

Quick-RNA™ FFPE Kit

Biological Fluids & Tissues

Quick-RNA™ Whole Blood Kit

Cells Quick-RNA™ Miniprep Kit

Direct-zol™ RNA Miniprep Plus Kit

Quick-RNA™ Miniprep Plus Kit

Samples in TRIzol®, TRI Reagent®, etc.

without phase separation in 7 min.

For RNA isolation in microbiomic studies, see our ZymoBIOMICS™

product line.

Learn more at http://www.zymoresearch.de/rna/rna-purifi cation

www.zymoresearch.deTel: +49 (0)761 60068710

Direct-zol™ RNA KitsHighlights

• Quick purifi cation of high-quality (DNA-free) total RNA directly from samples stored in TRIzol®, TRI Reagent® and all other acid-guanidinium-phenol based reagents.

• Eliminates phase separation and precipitation procedures.• Effi cient, broad range purifi cation of small and large RNAs from cells, tissues,

biological liquids, in vitro transcripts, etc.• Ideal for viral inactivation/sample storage.

Learn more and view additional Direct-zol™ Kit formats at http://www.zymoresearch.de/rna/rna-purifi cation

Accommodates any Samplein TRIzol®, TRI Reagent®, etc.

Sample in TRIzol®Add binding agent

directlyto aspin-column

Bind RNA Eluted RNAready to use

Innovation. Pure & Simple.™

Effi cient Small RNA Recovery High-quality RNA

From TRIzol® to RNA in only 7 minutes!

Product Cat. No. Size

Direct-zol™ RNA Microprep KitR2060, R2061* 50 preps.

R2062, R2063* 200 preps.

Direct-zol™ RNA Miniprep KitR2050, R2051* 50 preps.

R2052, R2053* 200 preps.

Direct-zol™ RNA Miniprep Plus Kit

R2070T 10 preps.

R2070, R2071* 50 preps.

R2072, R2073* 200 preps.

Direct-zol™ 96 RNA KitR2054, R2055* 2 x 96 preps.

R2056, R2057* 4 x 96 preps. TRIzol® and TRI Reagent® are registered trademarks of Molecular Research Center, Inc.U.S. Patent No. 9,051,563 B2 and other pending patents. Direct-zol™ is a trademark of Zymo Research Corp.

*Supplied with TRI Reagent®. Magbead formats also available.

4000

2000

1000

500

200

25

[nt]

9.3 9.0 9.49.4 9.1 9.1 9.1 9.2 9.0 (RIN)

High RNA integrity number (RIN > 9; Bioanalyzer® (Aligent Technologies Inc.)) indicates high-quality RNA was purifi ed from human epithelial cells using the Direct-zol™ RNA Kit.

miRNA-Seq

mirV

ana

(log

10)

Direct-zol™ (log10)

2837 overlapped miRNA: r2 = 0.9706

nCounter800 overlapped miRNA: r2 = 0.9027

mirV

ana

(log

10)

Direct-zol™ (log10)

The data show RNA purifi ed from TRIzol® samples using the Direct-zol™ RNA Miniprep compared to an unbiased method (mirVana™, Ambion). Micro-RNA analysis was performed using miRNA-Seq (MiSeq®, Illumina) and a direct hybridization assay (nCounter®, Nanostring).

TRIzol® IN

RNA OUT


Tel: (949) 679-1190 www.zymoresearch.com

Quick-RNA™ Miniprep Plus Kit

Learn more and view additional Quick-RNA™ formats at http://www.zymoresearch.de/rna/rna-purifi cation

Highlights

• High-quality total RNA (including small/micro RNAs) from all tissues, cells, whole blood, and biological fl uids.

• Worry-free sample storage at ambient temperatures with provided DNA/RNA Shield™.

• DNA-free RNA is ready for use in any downstream application.• No organic denaturants!


Quick-RNA™ Miniprep Plus KitR1057 50 preps.

R1058 200 preps.

Quick-RNA™ Miniprep Kit(no Proteinase K)

R1054 50 preps.

R1055 200 preps.

Value

Versatility Ultra-pure, DNA-free RNA

High-quality total RNA is isolated from various tissue types using the Quick-RNA™ Miniprep Plus Kit. In comparison, RNA isolated from the same tissue types using three kits recommended by Supplier Q (Agilent 2200 TapeStation®; Red = low quality).

Live

r

Lung

Sple

en

Bra

in

Mus

cle

Hea

rt

Live

r

Lung

Sple

en

Bra

in

Mus

cle

Hea

rt

Quick-RNA™ Miniprep Plus Kit Kit 1 Kit 2 Kit 3Zymo Research Supplier Q

ü ü ü ü ü ü û û û ü û ü

M

RNA isolated with the Quick-RNA™ Miniprep Plus kit is DNA-free. Samples isolated with Supplier Q’s kit are provided for comparison. Total RNA was isolated from 106 human epithelial cells (with in-column DNase treatments for both kits). Each amplifi cation curve represents an average of three independent isolation experiments.

QualitySupplier QQuick-RNA™

DNAFree!

GenomicDNA

28S −

18S −

The Quick-RNA™ kits yield high quality total RNA. High levels of genomic DNA contamination are present in the preps from Suppliers Q but not with the Quick-RNA™ kits. Total RNA was isolated from human epithelial cells (sans DNase treatment).

ûüQuick-RNA™ Supplier Q

Small RNA (≥17 nt) recovery Yes No

DNase I included Yes No

gDNA removal column included Yes No

Proteinase K Yes* No

DNA/RNA Shield™ (for sample storage) Yes* No

*Quick-RNA™ Miniprep Plus Kit


Tel: (949) 679-1190 www.zymoresearch.com


Quick-RNA™ Whole Blood Kit R1201 50 preps.

Quick-RNA™ Whole Blood Kit


Highlights

• Purify high-quality total RNA (including small/micro RNAs) from whole and partitioned blood samples.

• Compatible with commonly used anticoagulants (i.e., EDTA, citrate, heparin).• DNA-free RNA is ready for use in any downstream application. DNase I

included.• Worry-free sample storage at ambient temperatures with provided DNA/

RNA Shield™.

Innovative, Streamlined Workfl ow

Whole BloodPlasmaSerum

PBMCs, Buffy Coat, etc.

DNA/RNA Shield™

(optional storage/transport at ambient temp.)

Concentrated Total RNA(including small & micro RNAs)

Purify and Concentrate(optional DNase I treatment)

SpinWash x2Elute

Isolate:SpinElute

Zymo-Spin™ IIICG Column

Zymo-Spin™

IC Column

Add Ethanol

Nucleic Acid Stabilization at Ambient Temperature for 30 Days

Prior to Purifi cation

RNA in blood is effectively stabilized in DNA/RNA Shield™ at ambient temperature.

20

30

0 3 7 14

Ct

18s rRNA

C-FOS

ActinIL1-β

30Days

25

20

30

25

20

30

25

5

15

10 18s rRNA

b-Actin

c-Fos

IL1-B

Blood



Quick-RNA™ FFPE Kit R1008 50 preps.

Quick-DNA/RNA™ FFPE Kit R1009 50 preps.

Quick-RNA™ FFPE Kit


Highlights

• High performance sample prep technology for high-quality total RNA (including small/micro RNAs) from FFPE tissue samples and sections.

• DNA-free RNA is ready for use in any downstream application. DNase I included.

RNA isolated with the Quick-RNA™ FFPE Kit is higher quality compared to Supplier Q procedures. Quality assessed by Agilent 2200 TapeStation®.

Zymo Supplier Q

High-quality RNA

RNA isolated using the Quick-RNA™ FFPE Kit is high quality and consistently outperforms RNA isolated using Supplier Q procedures (Avg ΔCt = 3.6) as depicted by the RT-PCR amplifi cation curves (n=4).

5000

2500

Zymo

Supplier Q

30 35 40

0

RFU

cycles

RNA

Improved RNA Recovery fromFFPE Samples

Streamlined, Innovative Workfl ow

$

$

FFPE Tissue

$

$Proteinase K Digestion

Deparaffi nization

Ultra-pure Total RNA

SpinWashElute

Zymo-Spin™

Column


Quick-RNA™ Environmental Kits


Are you isolating RNA from tough-to-lyse and environmental samples? We offer a variety of kits which feature our superior mechanical lysis, BashingBead™, technology. With these kits, RNA can be isolated from samples otherwise resistant to conventional lysis procedures, including solid tissues, plants, seeds, food, arthropods, Gram-positive and Gram-negative bacteria, yeast, fi lamentous fungi, unicellular or fi lamentous algae, and protozoa. The result is high-yield, high-quality RNA that is suitable for downstream applications such as RT-PCR and more.


Quick-RNA™ Fecal/Soil Microbe Microprep Kit R2040 50 preps.

Quick-RNA™ Fungal/Bacterial Microprep Kit R2010 50 preps.

Quick-RNA™ Fungal/Bacterial Miniprep Kit R2014 50 preps.

Quick-RNA™ Tissue & Insect Microprep Kit R2030 50 preps.

Quick-RNA™ Plant Miniprep Kit R2024 50 preps.

Innovation. Pure & Simple.™ Superior Recovery of RNA from Environmental Samples

Isolation of total RNA from 10 mg of a fresh leaf material (Nicotiana sp.) using the Quick-RNA™ Plant Miniprep Kit. Leaves were minced, then processed using a FastPrep®-24 instrument (MP Biomedicals). Samples 1 and 2 were loaded in 2x and 1x volume aliquots, respectively, and resolved in a 1% (w/v) nondenaturing agarose gel. RNA Millenium™ Markers (Ambion) were used as size standards.

Sample 1 Sample 2

DNase I DNase I*

RNALadder

Zymo Research

Supplier A

DNALadder

9.0 -

6.0 -

3.0 -

2.0 -

1.0 -

0.5 -

kb

- 10.0- 3.0

- 1.0

- 0.5

kb

Total RNA was isolated from equal amounts of E.coli cells containing plasmid DNA (pGEM®) using the Quick-RNA™ Fungal/Bacterial Microprep Kit or kit from Supplier A. The samples were resolved in a 2% (w/v) agarose gel. RNA Millenium™ Markers (Ambion) and ZR 1 kb DNA Marker (Zymo Research) were used.

* = genomic (> 10 kb) and plasmid (> 3 kb) DNA contaminationDNase I = samples treated with DNase I.

Pulverize Sample UsingZR BashingBead™ Lysis Tube

Eliminate DNA withZymo-Spin™ IIIC

Bind, Wash, Elute RNAwith Zymo-Spin™ IIIC

Polyphenolic Removalwith Zymo-Spin™ IV-HRC

Clean & Concentrate RNAwith Zymo-Spin™ IC or IIC

Inhibitor-free RNA


Innovation. Pure & Simple.™

Since its inception in 1994, Zymo Research has been proudly serving the scientific community by providing innovative, reliable, and high quality research tools at affordable prices. Our vision... “The Beauty of Science is to Make Things Simple” is now truer than ever. Whether it’s epigenetics, DNA, RNA, E. coli, or yeast based research, our philosophy remains the same: To provide the highest quality products in the industry while ensuring they are both simple to use and reliable in their performance.

Zymo Research stands on three pillars which form the foundation of our company: Innovation, Quality, and Customer Service. These pillars are fundamental to our culture and ensure our products meet your needs.

InnovationZymo Research is historically recognized for its innovation of high quality nucleic acid purification technologies. Under the branding DNA Purification Made Simple® and RNA Purification Made Simple®, our technologies are pushing the limits of what is possible with nucleic acid isolation. As The Epigenetics Company™, Zymo Research has also received much attention for its rapidly expanding portfolio of epigenetics products and services. It is our objective to develop and provide the most comprehensive set of tools for DNA, RNA, and epigenetic research and analysis available today. Thousands of peer-reviewed scientific publications from researchers around the world feature our technologies. Through innovation, our scientists have made streamlined DNA methylation detection possible, pioneered the micro-elution column for DNA and RNA purification, developed the simplest and the most sophisticated methods for high-quality plasmid DNA purification, and patented the first RNA purification directly from Trizol® without phase separation among many other leading technologies in the industry.

QualityWe are committed to quality and guarantee that all of our products and service will meet and exceed your expectations. Our products are constantly evaluated by scientists like you to help ensure their reliability and the highest standard of quality.

Customer ServiceWe strive for excellence in how we support your scientific endeavors. We pledge to be honest and responsible for everything we do with you. We will treat you as we would like to be treated. Together, we will build a brighter future.

Tel: +49 (0)761 60068710 www.zymoresearch.de

Cat. No. B1003 Version 01

Documents

RNA from Any Sample - donar.messe.dedonar.messe.de/.../2017/F753061/rna-from-any-sample-eng-519881.pdfRNA from Any Sample Tel: ... High levels of genomic DNA contamination are present