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BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 – 60 per cent 61 – 80 per cent 81 – 100 per cent B_ B® Bk B¤ B® B B. B B BN

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Text of BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · 0 per cent 1 – 20 per cent 21 – 40 per cent 41 –...

  • 1

    2010 12 15

    ( ), ( , PWRI)Andrew JOHNSON

    (Centre for Ecology and Hydrology, CEH)

    22

  • 2005 2009•

    • Roach

    EDC

    2

  • 0 per cent

    1–20 per cent

    21–40 per cent

    41–60 per cent

    61–80 per cent

    81–100 per cent

    Jobling et al., 2006)

    3

  • 17�-estradiol (E2)

    Estrone (E1)

    OH

    OH

    O

    OH

    Ethynylestradiol EE2)

    OH

    OH

    ng/L4

  • 0

    2

    4

    6

    8

    10

    12

    14

    16

    18

    England Japan

    Nor

    mal

    ised

    exc

    retio

    n E

    2 eq

    uiv

    ug/h

    ead/

    d

    2%( , 2001)

    %

    E2 (Johnson et al., 2006

    0.6 %

    ?

    ? ? ?

  • 6

    1)2)3)

    UK-J Phase 2 CORE 1 (2005 to 2009)

  • Estradiol (E2) Estriol (E3) Estrone(E1)

    ( or

    Ethynyl estradiol (EE2)

    O

    C ONa

    OH

    HOOH

    OOH

    SO

    O

    OH

    OH

    7

  • Sample

    SPE

    (GF/B)

    Oasis HLB

    STD

    NH2 Cartridge

    CH3CN/H2O(1:9),1ml

    MeOH 0.5% NH4OH/MeOH

    N2 Evap.

    LC-MS/MS LC-MS/MS

    Clean up

    Clean-up with NH2

    Change LC 50 mm 100 mm

    : 500 ml / : 1000 ml

    8

  • 0

    10

    20

    30

    40

    50

    60

    70

    80

    90

    100

    E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G

    Con

    cent

    ratio

    n (n

    g/L)

    Influent Effluent

    UK (n = 3)

    � E1, E2 EE2

    =Range

    9

  • � EE2� E1� E1

    n=29

    0

    10

    20

    30

    40

    50

    60

    70

    80

    90

    100

    E1 E2 E3 E1-3S E2-3S E3-3S E1-3G E2-3G

    Con

    cent

    ratio

    n (n

    g/L)

    0

    10

    20

    30

    40

    50

    60

    70

    80

    90

    100

    Rem

    oval

    effi

    cien

    cy (

    %)

    Influent Effluent Removal

    10

  • 0

    20

    40

    60

    80

    100

    E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G

    Rem

    oval efficien

    cy (%

    )

    Removal efficiency

    -80

    -60

    -40

    -20

    0

    20

    40

    60

    80

    100

    E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G

    Rem

    oval

    effi

    cien

    cy (%

    )E2,EE2

    E1

    Jap

    an (

    n=

    9)U

    K (

    n=

    3)

    =Range

    11

  • 12

    � E1 EE2E2

    � EE2

    0

    10

    20

    30

    40

    50

    Con

    cent

    ratio

    n (n

    g/L)

    Influent

    Effluent

    Downstream

    E1 E2 E3 EE2 E1-3S E2-3S E3-3S E2-3&17GE3-3&17G

    Co

    nce

    ntra

    tion

    (ng

    /L)

  • 13

    Benson Sandhurst

    R. Thames Camberley

    Parking

    Sandhurst

  • 14

    E2?

    PST Reactor FST

    Qs

    Qs Qs

    PST Reactor FST

    Qs

    Qs

    kQs

    Qs

    kQs

    +

  • SRT

    15

    SRT=22E1 E2

  • �� ..

    N=6

    0

    2

    4

    6

    8

    10

    12

    14

    16

    18

    20

    E1 E2 E3 E1-3S E2-3S E3-3S

    Con

    cent

    ratio

    n (n

    g/L)

    0

    20

    40

    60

    80

    100

    Rem

    oval

    effi

    cien

    cy (

    %)

    Secondary Effluent After Ozonation Removal

    16

  • D7D4

    D5

    D3

    D2

    D9

    D8D6

    D1

    U2

    U1

    K1

    U9 U6

    U7

    U9U10

    K7

    K9K8

    K6K5

    K10

    K4K2

    K3

    U3 U4

    U9

    17

    �E2 EE2 , E1�E1 ,�E1

  • (segment)

    (segment)

    STP

    STP

    STP

    60

    , , ,

    18

  • 0

    1

    2

    3

    4

    5

    6

    7

    8

    9

    10

    Fro

    me

    Dar

    t

    Tor

    ridge

    Cuc

    kmer

    e

    Gre

    at S

    tour

    Aru

    n

    Soa

    r

    Tem

    e

    Cro

    uch

    Cam

    Wea

    r

    Tee

    s

    Ede

    n

    Cal

    der

    Wyr

    e

    Taf

    f

    Tyw

    i

    Eas

    tern

    Cle

    ddau

    Con

    wy

    Mer

    sey

    Irwel

    l

    Aire

    Don

    Bly

    th

    Ere

    was

    h

    Tre

    nt

    Idle

    Littl

    e O

    use

    Wav

    eney

    Tha

    mes

    Med

    way

    Avo

    n

    0

    1

    2

    3

    4

    5

    6

    7

    8

    9

    1075% 25%

    19

    EEQ (ng/L) E

    stim

    ated

    EE

    QE

    2-eq

    uiv

    alen

    t,n

    g/L

    ng/L

  • Risk Class

    No risk

    At risk

    High risk

    LF2000-WQX

    (>1 ng/L EEQ)

    (>10 ng/L EEQ)20

  • 0.0

    0.5

    1.0

    1.5

    2.0

    2.5

    3.0

    3.5

    4.0

    4.5

    5.0R

    umoi

    Sar

    u

    Tok

    oro

    Ishi

    kari

    Aka

    Yon

    esiro

    Kita

    kam

    i

    Tsu

    rum

    i

    Kuj

    i

    Nak

    a

    Kak

    ehas

    hi

    Kur

    obe

    Ara

    Shi

    nano

    Kik

    u

    Abe

    Syo

    unai

    Kis

    o

    Kita

    Yam

    ato

    Yur

    a

    Ash

    ida

    Asa

    hi

    Gou

    no

    Dok

    i

    Mon

    obe

    Niy

    odo

    Yos

    hino

    Hom

    myo

    Kim

    otsu

    ki

    Ooi

    ta

    Chi

    kugo

    0.0

    0.5

    1.0

    1.5

    2.0

    2.5

    3.0

    3.5

    4.0

    4.5

    5.0

    1ng/L

    30.6 18.0

    98Summer

    98Autumn

    99Spring

    99Summer

    99Autumn

    99Winter

    00Autumn

    01Autumn

    NA NA NA NA NA

    NA:No available data

    21

    Est

    rog

    enic

    Act

    ivit

    ies,

    E2-

    equ

    ival

    ent,

    ng

    /LEEQ

    (ng/L)

    YES Assayw/ hER

  • 22

  • •• k=0.067 /h

    0

    1

    2

    3

    4

    0 1 2 3 4

    Estimated Concentration (ng/L)

    Mea

    sure

    d C

    once

    ntra

    tion

    (ng/

    L)

    Theoretical line (x=y)

    23

  • 24

  • � SRT E1 , O3.

    25

    SRT + O3

    E1

    25

  • • EE2E2

    • SRT

    Summary

    26

  • 27

    Acknowledgements

    12th Annual Scientific Workshop UK-Japan Co-operation for Research on Endocrine Disrupters in the Aquatic Environment held at Otterburn HallNorthumberlandNational Park UK on 2-4 November 2010