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Influence of trace elements in nitrification

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• Introduction

• Nitrification: What is it and why is it important?

• Problems met by OASEN Company

• Batch experiment

• Conclusions

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Different ways to remove ammonium:

• Break point chlorination (not used in the Netherlands)

• Adsorption of ammonium on zeolites, ions exchange

• Stripping

• Biological conversion: Nitrification

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Nitrification

• Biological oxydation of ammonium into nitrite and then nitrate.

• NH3 + 1,5 O2 + nitrosomonas NO2- +H2O + H+

NO2- + 0,5 O2 + nitrospira NO3-

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Why is it important?

• pH > 8 => NH3 formation

• Nitrite formation Poisonous for fish Blue Baby syndrom

• NH4: Substrate for many bacteria

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Ammonium in filter effluent

0

1

2

3

4

5

6

Oct, 1997 Oct, 1998 Oct, 1999 Oct, 2000Am

mo

niu

m i

n f

ilte

r e

fflu

en

t [

pp

m N

H

4 ]

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Batch experiment

*Aeration*T=12°C*Shaking

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The results expected:

• NH4+ decrease more quickly in bottles with trace

elements.

• NO3- and NO2

- increase more in bottles with trace element than in bottles without trace elements.

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NO3- and NO2

-

Bach experiment: (N-NO3 + N-NO2)in in 2nd filtrate Lekkerkerk

0

2

4

6

8

10

12

0 5 10 15 20 25

time (days)

(N-N

O3 +

N-N

O2)

(mg

)

without traceelements

with traceelements

Bach experiment: (N-NO3 + N-NO2) in first filtrate ZS Kamerik

0

0,5

1

1,5

2

2,5

3

3,5

4

0 5 10 15 20 25

time (days)

(N-N

O3 +

N-N

O2)

(mg

)

without traceelements

with traceelements

(N-NO3 + N-NO2) in 2nd filtrate of ZS Ridderkerk

0

1

2

3

4

5

6

7

8

0 5 10 15 20 25

time (days)

(N-N

O3 +

N-N

O2)

(mg

) without traceelementswith traceelements

Bach experiment: (N-NO3 + N-NO2) in Lekkerkerk drinking water

0

0,5

1

1,5

2

2,5

3

3,5

4

0 5 10 15 20 25

time (days)

(N-N

O3+

N-N

O2)

(mg

)

without traceelements

with traceelements

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Total nitrogentotal nitrogen in Lekkerkerk drinking water

0

2

4

6

8

10

12

14

16

0 5 10 15 20 25

time (days)

tota

l nit

roge

n (m

g)

without traceelements

with trace elements

total nitrogen in Lekkerkerk 2nd filtrate

02

46

810

1214

16

0 5 10 15 20 25

time (days)

tota

l nit

roge

n (m

g)

without traceelements

with trace elements

total nitrogen in first filtrate ZS Kamerik

02468

10121416

0 5 10 15 20 25

time (days)

tota

l nit

roge

n (m

g)

without traceelements

with trace elements

total nitrogen in 2nd filtrate Ridderkerk

02468

10121416

0 5 10 15 20 25

time (days)

tota

l nit

roge

n (m

g)

without traceelements

with trace elements

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Second experiment

*T =20°C*Shaking*Single limitation

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Results

Batch experiment: (NO3 + NO2)-N in Lekkerkerk 2nd filtrate

05

101520

0 2 4 6 8

time (days)

(NO3

+ N

O2)-N

(m

g)

without Co

with all traceelements

without traceelements

Batch experiment: NH4-N in Lekkerkerk 2nd filtrate

02468

1012

0 2 4 6 8

time (days)(N

H4-N

) (mg

)

without Co

with all traceelements

without traceelements

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Total nitrogenBatch experiment: Total nitrogen in Lekkerker 2nd filtrate

02468

101214161820

0 2 4 6 8

time (days)

To

tal

nit

rog

en

(m

g)

without Co

without P

without Mo

without Cu

without Zn

with all traceelementswithout traceelements

h

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Conclusion

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Thank you for your attention,

Questions ?