1. 21 2556; 11(1): 21-29 Salmonella 1 , 1 , 2 , 3 , 4 , 4 4 * 1 2 3 4 Salmonella ISO 6579:2002 Salmonella 12 57.73 2.08 LogCFU/g Salmonella 45.45 Salmonella Norfloxacin, Ciprofloxacin Amoxicillin-clavulanic acid 100 Ampicillin 84.38, Tetracycline 74.48 Streptomycin 71.88 2556; 11(1): 21-29: Salmonella, , , : .. . 50200: E-mail address: patprapas@gmail.com 30 2555

2. 22 Salmonella spp. (Thorns, 2000) .. 2555 ( 1 26 ..2555) 105,028 ( , 2012) (Hedberg, 1999) Salmonella (International Associationfor Food, 2011) Salmonella Salmonella Salmonella Salmonella 20(Ruttayaporn Ngasaman, 2007) 12.3 (Phengjai Sangvatanakul, 2007) 28 (ArsoothSanguankiat, 2005) Salmonella Salmonella Salmonella Salmonella Salmonella Salmonella Salmonella (Cross-sectionalstudy) Salmonella Salmonella 6 805 606 3, 8, 12, 18 24 3. 23 Salmonella 199 100 Buffered Peptone Water (BPW) 1:10 25 Swab Technique Swab 100 4-8 24 ISO 6579:2002, Amendment 1:2007, Annex D 25 Buffered Peptone Water (BPW) 225 1:10 Stomacher 2 37 24 100 Modified Semi-solidRappaport-Vassiliadis (MSRV) 42 24 MSRV Xylose lysinedeoxycholate agar (XLD agar) Brilliant-green phenol-red lactose sucrose agar (BPLSagar) 37 24 Salmonella 100 Buffered Peptone Water(BPW) 1:10 37 24 100 Rappaport-Vassiliadis (RV broth) 10 1000 Tetrathionate (TT broth) 9 42 24 RV broth 37 24-48 TTbroth RV TT broth XLD agar BPLSagar 37 24 Salmonella Salmonella Most Probable Number (MPN) 3 Salmonella (The National Salmonella andShigella Center) 95 (95%Confident Interval) PH Stat 2.7(Add-In for Microsoft Excel 2010) (Average Log CFU/g) Salmonella @Risk 5.5 (PalisadeCorporation 798 Cascadilla Street Ithaca, NewYork 14850 U.S.A.) Fit distribution to 4. 24 data Anderson-Darling Statistics Salmonella 12 57.73 24 40.62 40.32 45.45 42.11 Salmonella Salmonella @Risk 5.5( 1) 1 Salmonella (-) (LogCFU/g) @Risk 5.5 Fit distribution to data 5 Salmonella MPN 5 @Risk 5.5 5. 25 Salmonella 20 SalmonellaRissen 27.60 SalmonellaTyphimurium 16.15 ( 1) Salmonella 1 Salmonella 6. 26 10 Ampicillin (AMP) 10 g, Amoxicillin-clavulanic acid (AMC) 30 g, Chloramphenicol(C) 30 g, Ciprofloxacin (CIP) 5 g, Cefotaxime(CTX) 30 g, Nalidixic acid (NA) 30 g,Norfloxacin (NOR) 10 g, Streptomycin (S)30 g, Tetracycline (TE) 30 g Sulfamethoxazole-trimethoprim (SXT) 25 g Salmonella Norfloxacin,Ciprofloxacin Amoxicillin-clavulanic acid 100 Ampicillin,Streptomycin, Tetracycline ( 2) 2 Salmonella Salmonella Salmonella 12 57.73 (56/97, 95% CI:47.90-67.56) 2.08 Log CFU/g @Risk 5.5 (Fit distribution todata) ExtValue 24 40.62 (39/96, 95%CI:30.80-50.45) 40.32 (25/62, 95% CI:28.11-52.53) 1.98 2.36 LogCFU/g Logistic LogLogistic Phengjai(2007) Salmonella Salmonella 12.3 (Phengjai Sangvatanakul, 2007) 30 (24/80,95% CI: 19.95-40.04) 2.07 Log CFU/g Ruttayaporn (2007) Salmonella 20 7. 27 10 3 33.75 (27/80, 95% CI: 23.38-44.11) 1.97Log CFU/g 8 14.73 (14/95, 95% CI: 7.6-21.86) 1.68 Log CFU/g 3 8 ExtValue Expon Salmonella 45.45(5/11, 95% CI:16.03-74.88) 42.11 (8/19, 95% CI:19.90-64.30) 2.55 LogCFU/g 26.67 (8/30, 95%CI:10.84-42.49) 2.58 Log CFU/g 6.67 16.67 2.28 Log10CFU/g Lognorm, Uniform Normal Salmonella Swine high fever syndrome Highly-pathogenic-PRRS (Biosecurity system) Salmonella Salmonella 3 Norfloxacin,Ciprofloxacin Amoxicillin-clavulanic acid 100 Salmonella Ampicillin 84.38, Tetracycline 74.48 Streptomycin 71.88 ( 2) Salmonella Salmonalla Salmonella Salmonella Salmonella (Montecarlo simulation) Foodsupply chain (, , ) (Risk) (from farm to table) Salmonella 8. 28 (.) ( P-10-10409) . Annual epidemiological surveillance report 2012. Retrievedfrom http://www.boe. moph.go.th/boedb/d506_1/index.php ArsoothSanguankiat. (2005). A Cross-sectional study of salmonella in pork products in Chiang Mai, Thailand = . Thesis (Master of Veterinary Public Health) Chiang Mai University; FreieUniversitat Berlin. Hedberg, C. (1999). Food-related illness and death in the United States. Emerging infectious diseases, 5(6), 840842.doi: 10.3201/eid0505.990502 International Association for Food. (2011). Procedures to Investigate Foodborne Illness (6thed.). New York: Springer. PhengjaiSangvatanakul. (2007). Prevalence of salmonella in piglets and in the fattening period in Chiang Mai, Thailand = . Thesis (Master of Veterinary Public Health) Chiang Mai University; FreieUniversitat Berlin. RuttayapornNgasaman. (2007). Prevalence of salmonella in breeder sows in Chiang Mai, Thailand = . Thesis (Master of Veterinary Public Health) Chiang Mai University; FreieUniversitat Berlin. Thorns, C. J. (2000). Bacterial food-borne zoonoses. Revue scientifiqueet technique (International Office of Epizootics), 19(1), 226239. 9. 29 Salmonella Human-Animal-Environment Interface of Salmonella Contamination in Swine farms Kittipong Kumpapong1 , Danai Sinthuya1 , Nattakarn Awaiwanont2 , Suphachai Nuanualsuwan3 , Panuwat Yamsakul4 , Pakpoom Tadee4 and Prapas Patchanee4 * 1 Master student in Veterinary Science, Faculty of Veterinary Medicine, Chiang Mai University 2 Veterinary Public Health Centre for Asia Pacific, Faculty of Veterinary Medicine, Chiang Mai University 3 Department of Veterinary Public health, Faculty of Veterinary Science, Chulalongkorn University 4 Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University Abstract The purpose of this study was to determine the interface and to quantify Salmonellacontamination in swine farms. Pigs fecal samples were collected from all groups stage, aswell as samples from farms environment. Samples collected were cultured in a laboratoryfor Salmonella contamination detection using conventional microbiological methodfollowing ISO 6579:2002. The findings revealed that, the highest Salmonella contamination wasin pigs age 12 weeks at 57.73%. The average of Salmonella contamination in fecal sampleswas at 2.08 LogCFU per grams. From the environmental samples, flies were found to containingthe highest average of Salmonella contamination at 45.45%. Salmonella showed the highestantimicrobial susceptibility for Norfloxacin, Ciprofloxacin and Amoxicillin-clavulanic acid at100% and the highest resistant Ampicillin (84.38%), Tetracycline (74.48%) and Streptomycin(71.88%).Key words: Salmonella, Contamination, Human-Animal-Environment, Swine farms