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Spring 2010 introduction to microscopy PowerPoint for BIOL2401 (Human Anatomy & Physiology) at San Antonio College with Alba.
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Microscopy 101
Proper Handling
• Make sure cord is secure
• Support the base with one hand
• Lift with the arm of the scope
• Do not swing!
Ocular
Arm
Stage
Base
Coarse Adjustment
Fine AdjustmentLight Source
Diaphragm
Stage Clips
Objectives
Nosepiece
Tube
Magnification
Ocular = 10x
4x Objective 10x Objective 40x Objective
Therefore, 40x, 100x, 400x Magnification
Getting Started
• Plug in & turn on
• Rotate nosepiece to place 4x objective into position
• Raise stage all the way up by turning the coarse adjustment knob
• Place specimen slide into place
• Look into ocular as you lower the stage VERY SLOWLY until low magnification image focuses
Increasing Magnification & Focusing
• Once low magnification is focused, DO NOT touch coarse adjustment knob ever again!
• Switch to 10x objective by rotating nosepiece
• Use fine adjustment knob to focus
• Once focused, switch to 40x objective to view high magnification
• Focus with fine adjustment knob
Before you leave…
• Return slides to proper boxes
• Turn off & unplug scopes
• Rotate to lowest objectives (4x)
• Raise stage all the way up with coarse adjustment knob
• Secure cords
• Return to cabinets, carrying properly