Download pdf - Polyclonal ANTIBODY GCLM · Immunogen Catalog Number: AG5497 GenBank Accession Number: BC041809 GeneID (NCBI): 2730 Full Name: glutamate-cysteine ligase, modifier subunit Calculated

GCLM Polyclonal antibodyCatalog Number:14241-1-AP 50 Publications

For Research Use Only

www.ptgcn.com

T: 4006900926 E: [email protected] W: ptgcn.comFor technical support and original validation data for this product please contact: This product is exclusively available under

Proteintech Group brand and is not available topurchase from any other manufacturer.

Basic Information Catalog Number:14241-1-APSize:300 μg/mlSource:RabbitIsotype:IgGPurif ication Method:Antigen aff inity purif icationImmunogen Catalog Number:AG5497

GenBank Accession Number:BC041809GeneID (NCBI):2730Full Name:glutamate-cysteine ligase, modif ier subunitCalculated MW:31 kDaObserved MW:31 kDa

Recommended Dilutions:WB 1:1000-1:4000 IP 0.5-4.0 ug for IP and 1:500-1:2000 for WBIHC 1:100-1:400 IF 1:20-1:200

Applications Tested Applications:FC, IF, IHC, IP, WB, ELISACited Applications:IF, IHC, WBSpecies Specif icity:human, mouse, ratCited Species:human, mouse, ratNote-IHC: suggested an gen retrieval withTE buffer pH 9.0; (*) Alterna vely, an genretrieval may be performed with citratebuffer pH 6.0

Positive Controls:WB : HeLa cells; MCF-7 cells, L02 cells, A431 cells,mouse liver tissue, rat liver tissueIP : A431 cells;IHC : human liver cancer tissue; human skeletalmuscle tissueIF : HepG2 cells;

Background Information GCLM(Glutamate--cysteine ligase regulatory subunit) is also named as GLCLR and belongs to the aldo/keto reductase family. It catalyzes the f irst step ofGSH synthesis and is involved in gamma-glutamyl cycle. GCLM is implicated in some forms of hemolytic anemia.

Notable Publications Author Pubmed ID Journal Application

Jiawen Zheng 31569771 Mar Drugs WB

Xiaoying Tang 31548295 Mol Cancer Ther

Yijun Tian 27601007 J Hematol Oncol WB

Storage Storage:Store at -20ºC. Stable for one year af ter shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for -20ºC storage

Selected Validation Data

Various lysates were subjected to SDS PAGEfollowed by western blot with 14241-1-AP (GCLMantibody) at dilution of 1:1000 incubated at roomtemperature for 1.5 hours.

Immunofluorescent analysis of HepG2 cells,using GCLM antibody 14241-1-AP at 1:50 dilutionand Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (f luorescent DNA dye).

IP Result of anti-GCLM (IP:14241-1-AP, 3ug;Detection:14241-1-AP 1:1000) with A431 cellslysate 3040ug.

Immunohistochemical analysis of paraff in-embedded human liver cancer tissue slide using14241-1-AP (GCLM Antibody) at dilution of 1:200(under 10x lens).

Immunohistochemical analysis of paraff in-embedded human liver cancer tissue slide using14241-1-AP (GCLM Antibody) at dilution of 1:200(under 40x lens).

1X10̂ 6 HepG2 cells were stained with 0.2ugGCLM antibody (14241-1-AP, red) and controlantibody (blue). Fixed with 90% MeOH blockedwith 3% BSA (30 min). Alexa Fluor 488-conjugatedAff iniPure Goat Anti-Rabbit IgG(H+L) with dilution1:1500.