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BioFactoryTM
WhiteSciWhitehead Scientific (Pty) Ltd
Products. Expertise. Support.
Your Best Choice For Laboratory ConsumablesFounded in 2009, Wuxi NEST Biotechnology is a worldwide manufacturer of plastic laboratory goods. State-of-the-art
machinery is used on a 100,000-class dust-free manufacturing site based in Wuxi, China, which has been certified with an
ISO 9001:2008 management system. Our manufacture procedures meet the ISO 11137 and ISO 13485 standards. NEST
products are also registered with the CE and the FDA.
ISO 11137 ISO 9001 ISO 13485 FDA
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Environment – 10,000-class Clean Room For Production
The NEST BioFactoryTM line is produced in a dedicated 10,000-class clean room, and other NEST products are produced in a
100,000-class clean room.
Raw Material
BioFactoryTM Body – Virgin Polystyrene, meets USP Class VI requirements for plastic containers and closures
BioFacrtoryTM Caps – HDPE (High Density PolyEthylene)
Filter membranes in the caps – PTFE (Poly Tetra FluoroEthylene)
Surface Treatment – Tissue Culture Treated
The surface of the BioFactoryTM vessel has been through hydrophilic treatment to ensure more uniform and stable cell adherence.
Validity Verification
The cell growth meets all the requirements after three years of aging, and passes the sterility tests with all negative results.
Biosafety Test
BioFactoryTM is tested for cytotoxicity, sensitization, intradermal stimulation, acute systemic toxicity and hemolysis referring to the
"Compilation of National Standards for Packaging Materials and Containers Directly in Contact with Drugs (Sixth Series)".
Physical And Chemical Safety Test
BioFactoryTM is tested for insoluble particles, ignition residue, metal elements, and dissolution (clarity, color, pH, UV absorbance,
non-volatiles, oxides, heavy metals) referring to the "Compilation of National Standards for Packaging Materials and Containers
Directly in Contact with Drugs (Sixth Series)".
Cell Lines That Grow Well With BiofactoryTM
VERO、MRC-5、2BS、293T、L-929、MSE、MSC……
Sterility Protection – In-House Sterilization Is Performed By Rhodotron TT200 From Belgium
IBA, A World Leader In The E-beam Sterilization Industry.
The sterilization procedures meet the ISO13485 and ISO 11137:2006 standards.
Strict Product Quality Supervision
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NEST BioFactoryTM
NEST BioFactoryTM system is a well-designed cell culture device that utilizes the maximum culture area in a limited space, saving a large
amount of space, low-cost to achieve the purpose of expanding production capacity.
NEST BioFactoryTM system can be used for industrial scale production such as vaccine, monoclonal antibodies or biopharmaceuticals
production, and they are suitable for adherent cell culture.
Specifications: Single layer, 2 layers, 5 layers, 10 layers and 40 layers.
3
NEST BioFactoryTM has been widely used in large-scale culture of cell lines such as VERO, MRC-5, 2BS, 293T,
L-929, MSE and MSC.
Double Wide Mouth, Filter Vent Caps
771001
771101
771204
771302
771403
1
2
5
10
40
647
1279
3175
6335
25295
335
335
335
335
335
205
205
205
205
205
44
61
112
197
712
8
8
4
6
2
Cat.No. Layer Cultivation area(cm²) Length(mm) Width(mm) Height (mm) /Case
Tip:
BioFactoryTM Advantage
Double Narrow Mouth, (One Filter Vent Cap & One Plug Seal Cap)
772001
772101
772204
772302
772403
1
2
5
10
40
647
1279
3175
6335
25295
8
8
4
6
2
335
335
335
335
335
205
205
205
205
205
44
61
112
197
712
One Wide Mouth Filter Vent Cap & One Narrow Mouth Plug Seal Cap
773001
773101
773204
773302
773403
1
2
5
10
40
647
1279
3175
6335
25295
8
8
4
6
2
335
335
335
335
335
205
205
205
205
205
44
61
112
197
712
Made of Virgin Polystyrene, which meets USP Class VI requirements for plastic containers and closures;
Manufactured in dedicated 10,000-class clean room following GMP standards to ensure product quality and stability;
Ultrasonic welding process without any chemical addition;
Surface TC treatment ensures efficient cell attachment;
In-house E-beam sterilization meets the ISO13485 and ISO 11137:2006 standards and achieves sterility level SAL = 10-6;
Independent double-layer sterile packaging;
Compatible with Corning® CellSTACK® Culture Chamber accessories.
Large mouth design for easy pouring of the medium directly;
The small mouth design for easy connection with the feeding systems;
Breathable cover: 0.22um hydrophobic gas permeable membrane, hindering bacteria and water, can also avoid liquid swelling.
Cat.No. Layer Cultivation area(cm²) Length(mm) Width(mm) Height (mm) /Case
Cat.No. Layer Cultivation area(cm²) Length(mm) Width(mm) Height (mm) /Case
4
742001 Vent Filter
0.22 um，50 mm diameter
743001 Hose Clamp
Hose clamp for 12-18 mm
diameter hose
740001
740011
740101
740111
Wide Mouth Seal Cap For Biofactory
Wide Mouth Vent Cap For Biofactory
Narrow Mouth Seal Cap For Biofactory
Narrow Mouth Vent Cap For Biofactory
HDPE
HDPE
HDPE
HDPE
1
1
1
1
10
10
10
10
Cat.No. Name Material /pack /case
740201 Adaptor Cap Wide Mouth to Narrow Mouth
Adaptor Cap Wide Mouth to 3/8 Inches(9.5 mm)hose
1 10
1 10
14.311.8
spec (mm)
The inside diameter The outside diameter
741001 Adaptor Connector
Adaptor connector to 3/8 Inches
(9.5 mm) hose
744001 SPT-50 Hose
Inner Dia# 3/8 Inches (9.5 mm
Out Dia# 5/8 Inches (15.9 mm
50 Inches/ 15 meters
747001 CPC Connector
CPC & PC
(Inner Dia #3/8) for hose to hose connecting
751001 Y Shape CPC Connector
Y Shape CPC Connector for Inner Dia
#3/8 Hose
749001 T Shape CPC Connector
T Shape CPC Connector for Inner Dia
#3/8 Hose750001 Funnel For Wide Mouth
Upper Dia 60mm, Lower dia 24mm
746001 C-Flex Welding Hose
Inner Dia# 3/8 Inches (9.5 mm)
Out Dia# 5/8 Inches (15.9 mm)
50 Inches/ 15 meters
BioFactoryTM Accessories (Sterile)
740201 740301
745001 Combination Packages
Each set contains：
740201 Vent Filter/ 1
740301 Adaptor Cap Wide Mouth to 3/8 Inches (9.5 mm) hose/1
743001 Hose clamp for 12-18 mm diameter hose/1
741001 Adaptor connector to 3/8 Inches (9.5 mm) hose/1
742001 Vent Filter/1
744001 SPT-50 hose/1 meter
HDPE
HDPE
Cat.No. Name Material
740301 7.3 11.4
/pack /case
5
Cell culture
Precautions
Precaution
Cell harvest
5.Put the BioFactoryTM into the incubator.
Guidelines For Use
1、
2、
2.Turn the BioFactoryTM Chamber 90° to keep the liquid level at the level.
3.Turn the BioFactoryTM Chamber 90° so that the filling and venting ports are up (as shown). It is normal for the medium level in the bottom chamber section to be slightly higher.
4.Gently lower the BioFactoryTM Chamber to its normal horizontal incubation position and gently tilt the chamber back and forth until the surface of each chamber is completely covered with medium.
Continued mixing of the cell suspension (gently as to not create foam) when filling chambers is recommended to ensure even cell distribution. Preheating the medium to incubation temperature is also recommended to ensure rapid cell attachment. For larger BioFactoryTM Chambers, prewarming the Chamber and the medium is especially important due to the increased time required for the middle chambers to reach desired incubation temperatures.Be careful not to spray the hydrophobic filters on the venting caps with alcohol as this will wet them and potentially block the gas flow resulting in pressure build-up or preventing gas exchange.
For enzymatic cell harvesting using trypsin or other dissociating enzymes, NEST recommends washing the chamber with calcium and magnesium-free phosphate buffered saline (40 to 50 mL per chamber) at least once to remove any serum containing medium residues. Then add prewarmed dissociating solution (20 to 30 mL per chamber) to the chamber and carefully tilt the chamber back and forth and side to side to ensure the entire cell surface is completely wetted with dissociating solution.
Check temperature of shelves as well as the air at different levels in walk-in warm rooms. A 1°C to 2°C variation from the required optimal cell growing temperature is common in many warm rooms and may decrease desired yield significantly.
1.Pour the prepared suspension into the BioFactoryTM (recommended volume of 150-200 ml per layer).
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Cart
NEST BioFactoryTM AutoHandlerTM
Maximize Productivity With Automation SolutionsThe NEST BioFactoryTM AutoHandlerTM is a commercial-scale system that fully automates the handling of NEST BioFactoryTM and
delivers the solution for scale-up of your existing BioFactoryTM systems. NEST BioFactoryTM AutoHandlerTM has automated the filling,
emptying, harvesting, and trypsinization steps of BioFactoryTM systems. All functions can be activated by the programmable motion
controller, eliminating manual handling and realizing process consistency, optimization and standardization.
· NEST BioFactoryTM AutoHandlerTM is made of stainless steel, no rubber transmissions.
· Designed for use in clean room environments
· Each AutohandlerTM can hold four of NEST BioFactoryTM 40 Layers, or twelve of NEST BioFactoryTM 10 Layers
Load Tilt back: 0-35°
Tilt forward: 0-20° It can rotate to the left or right. And it can tilt forward or backwards while rotating.
Please check out our new BioFactory Automated Shaker!! It will handle four of our 40-layer BioFactories and twelve of our 10-layers!https://youtu.be/-X3sA3PbGJQ
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Other Products
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PCR Plates
PCR 8-Strip Tubes
Vacuum Filtration SystemsPET Storage Bottles Petri DishesInoculating Loops & Needles& L-Spreader
Syrings Filters
15/50mL Centrifuge TubesMicro Centrifuge TubesSerological Pipettes Screw Cap Tubes
50mL Mini Bioreactor Erlenmeyer Flasks5-layer Cell Culture FlaskCell Culture Flasks Cryogenic Vials
Cell Culture Plates Glass Bottom Culture Dishes/PlatesEDGE Plates Culture Insert Cell Culture Dishes
ELISA Plates Deep Well Plates
PC Erlenmeyer Flasks, High Efficiency
785101
785111
787001
787011
2
2
3
3
5
5
Seal Cap
Vent Filter Cap
Seal Cap
Vent Filter Cap
Seal Cap
Vent Filter Cap
213.5
213.5
253.5
253.5
285.5
285.5
90
90
230
230
4
4
4
4
Size(mm)Height
Cat.No. Volume L Cap StyleBottom Diameter
/CaseBottleneck Diameter
FeaturePC Bottle & HDPE CapVent caps with 0.2um hydrophobic filters for gas exchange without contamination.
Sterilized by E-BeamNonpyrogenic
2L PC Erlenmeyer Flasks, High Efficiency 3L PC Erlenmeyer Flasks, High Efficiency 5L PC Erlenmeyer Flasks, High Efficiency
785931
787931
785921
787921
785941
787941
Thermoplastic Hose.
4
4
4
4
4
4
4
4
4
Hoses' Length(cm)Cat.No. Name Description of Hoses /Case
Closed system reduces the risks of contamination during liquid transfer.The cap is molded and connected in an integrated manner, reducing the risks of leakage and media residues.A variety of tubing diameters are available and aseptic welding of liquid inlet tubing under normal conditions is supported.High-quality materials and smooth inner wall of the tubing provide an excellent transfer performance.Sterility level (SAL) is maintained at 10-6. Endotoxin-free, and no ingredients of animal origin.
Feature
Application It is applicable to liquid transfer and culture during mass proliferation of bacteria and suspension cells.
Two-way Liquid Transfer Cap
Multifunctional Liquid Transfer Cap
Inverted Liquid Transfer Cap
Transfer Cap System
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Edition : Second edition of December 2018
Wuxi NEST Biotechnology Co.,Ltd
Tel : +86-510-6878 8698 / +86-510-6878 8718
E-mail : [email protected]
Web : www.nestscientificusa.com
Add : No. 230 Xida Road, New District, Wuxi, Jiangsu, China
NEST Scientific USA
Tel : +1-732 381 0268
E-mail : [email protected]
: www.nestscientificusa.com
Add : 1592 Hart St., Rahway, NJ 07065, USA
Web
GET YOUR QUOTE NOW: [email protected] | www.whitesci.co.za
WhiteSciWhitehead Scientific (Pty) Ltd
Products. Expertise. Support.
