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Chemistry and Light
There are lots of examples of where we use light and chemistry:
input output EXAMPLELight Reaction Photochemistry
Heat Light Flame tests
Reaction Light Chemiluminescence
Light Light (different colour)
fluorescence
The emission of light from a chemical, when it is irradiated with light of a different energy:
Substance ABSORBS energy from LIGHT – ‘excited’
Energy is released as LIGHT emission = fluorescence
This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.
Fluorescence (sometimes called photoluminescence)
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This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.
Today we are going to extract some fluorescent chemicals from household items.
To do this we will use some of the following chemistry equipment below – can you identify the items, and draw 2D representations of each?
This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.
Practical Experiment:
AIM: To make a selection of fluorescent solutions
ATTENTION: wear safety goggles – we are going to use ethanol
You will need:BeakerFilter paperFunnelConical flasksSpatulaEthanolWater
ACTIVITY
We are going to extract fluorescent molecules from some common items that you may come across at home:
Equipment for the extraction
DISSOLVE the fluorescent compound in a SOLVENT, in a beaker, to form a SOLUTION.
FITLER this solution to remove the RESIDUE, and collect the FILTRATE:
This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.
Make up a fluorescent solution of vitamin B2 and another solution of your choice:
1. Make up a fluorescent solution of riboflavin (vitamin B2):- Empty a capsule of vitamin B2 into a beaker, with and dissolve it in 50 mL of water,
and stir - Pour the mixture into a filter funnel, and collect the liquid in the funnel at the bottom- Use a pipette to collect some of this liquid into a sample vial, put the lid on it, and
look at this under the UV lightWhat light is it absorbing?What type of light is it emitting?
2. Or Make up a fluorescent solution of chlorophyll:- Take a handful of spinach leaves, put into sample bag- Pour enough ethanol into the bag to cover the spinach leaves- Scrunch the leaves in the bag and in the ethanol for a few minutes- Pour the mixture into a filter funnel, and collect the green liquid in the funnel at the
bottom- Use a pipette to collect some of this liquid into a sample vial, put the lid on it, and
look at this under the UV lightWhat light is it absorbing?What type of light is it emitting?
If you have time:
3. Make up a fluorescent solution of optical brightener:- Take a teaspoon of washing powder, and dissolve it in 50 mL of water- Pour the mixture into a filter funnel, and collect the liquid in the funnel at the bottom- Use a pipette to collect some of this liquid into a sample vial, put the lid on it, and
look at this under the UV lightWhat light is it absorbing?What type of light is it emitting?
4. Or Make up a fluorescent solution of curcumin:- Take a teaspoon of turmeric, and dissolve it in 50 mL of ethanol- Pour the mixture into a filter funnel, and collect the liquid in the funnel at the bottom- Use a pipette to collect some of this liquid into a sample vial, put the lid on it, and
look at this under the UV lightWhat light is it absorbing?What type of light is it emitting?
This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.
Helpful notes:
This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.