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Introduction. Results. Trial 1 : Effects of cattle diet on microbial community. Eubacteria Archaea. Trial 1 Seven, 1 L anaerobic digesters Cattle Diets: Control WDGS 82.5% DRC47.5% DRC 5% Molasses40% WDGS 7.5% Alfalfa7.5% Alfalfa 5% Supplement5% Supplement 0.986% Urea Trial 2 - PowerPoint PPT Presentation
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Methane Production via Anaerobic Digestion of Feedlot Manure
A.K. Watson, G.E. Erickson, S.C. Fernando, T.J. Klopfenstein, A.Schmidt
Trial 1Seven, 1 L anaerobic digestersCattle Diets: Control WDGS
82.5% DRC47.5% DRC5% Molasses 40% WDGS7.5% Alfalfa 7.5% Alfalfa5% Supplement 5% Supplement 0.986% Urea
Trial 2Cattle Housing: Open Lot Confinement Manure OM 26% 88%
Trial 3Ten, 45 L anaerobic digestersCattle Housing: “Pure” manure 80% OM Soil surface of pen 10% OM Stockpiled 20% OM Fresh 25% OM Cement pad 50% OM
Trial 1: Effects of cattle diet Trial 2: Effects of cattle housing
CONT WDGS SEM P-value CONF FDLT SEM P-value
DMD, % 42.7 44.9 1.06 0.05 39.0 19.9 2.79 < 0.01
OMD, % 51.0 52.9 1.14 0.10 46.7 24.8 3.11 < 0.01
Methane, L/d 0.551 0.634 0.05 0.10 0.478 0.229 0.07 < 0.01
Methane, L/g OM fed 0.116 0.137 0.01 0.05 0.103 0.189 0.03 0.01
Trial 1: Effects of cattle diet on microbial communityEubacteria Archaea
CONT
WDGS177 137
8747
-0.5 -0.4 -0.3 -0.2 -0.1 0 0.1 0.2 0.3 0.4 0.5
-0.5
-0.4
-0.3
-0.2
-0.1
0
0.1
0.2
0.3
0.4
0.5Principal Coordinate Analysis
Bacteria-WDGS Bacteria-CONTArchaea-WDGS Archaea-CONT
PC1 (23.6% - Bacteria, 53.9% - Archaea)
PC2
(15.
5% -
Bact
eria
, 14.
0% -
Arch
aea)
ConclusionsTrial 1• Manure from cattle fed WDGS had increased methane potential compared to corn
based CONT• Dietary changes can lead to changes in microbial community that provide
opportunities to alter methane production from cattle manureTrial 2• Open lot manure can be used as anaerobic digester feedstock if ash buildup is avoidedTrial 3—In progress• Strategically harvesting manure may enhance methane production from open lot
manure and allow the feedlot industry to implement anaerobic digestion of manure
Cement Pad
Soil surface
Introduction Results
Variation in Microbial CommunityHomova Archaea P < 0.001 Eubacteria P = 0.019Amova Archaea P < 0.001 Eubacteria P < 0.001