Laboratory Scale Anaerobic Digesters- Theory and Operation

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Laboratory Scale Anaerobic Digesters- Theory and Operation. By: Zachary Bryan Scott Department of Civil and Environmental Engineering University of California, Irvine. Motivation. Wastewater Treatment : Aerobic Biological Treatment of Wastewater Cleans wastewaters - PowerPoint PPT Presentation

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  • By: Zachary Bryan ScottDepartment of Civil and Environmental Engineering University of California, Irvine

  • Wastewater Treatment:

    Aerobic Biological Treatment of Wastewater Cleans wastewaters Generates Biomass (0.4g VSS/ g COD)

    Anaerobic Biological Treatment Cleans wastewaters Many Types Reduces biomass (0.05 g VSS/ g COD) Can produce net energy Consider heating, transport, 4 ft3 CH4 / lb VS as manure

    *

  • *Reactor Assessment/Optimization Design Process Modification

  • *Modify Reactor Conditions:pH, T, Feed Type, Feed interval, Mixing Intensity, NutrientsCurrent Industrial Use of Models is very limited Pilot testing still dominant = higher costs = more time

  • *Modified figure, Gujer and Zhender, 1979Anaerobic Process:

  • Three Critical Measurements:

    Methane Production Rates-FlowcellAcetate Consumption Rates-GCAceticlastic Methanogen Ribosomal DNA Copy # - qPCR

  • 6 Batch ReactorsHighly Degraded Manure-Media (MSL)Fed Acetic AcidAcrylic, Rod Mixed

    Glass, Shaker-Mixed

  • Acrylic, Rod Mixed

  • Glass, Rod-Mixed

  • How are Organisms Identified and Quantified in mixed culture?

    Each taxonomic group has ribosomal DNA (rDNA) that is unique to its groupThis information is available in public databases. ex: NCBI

    rDNA is extracted from samples

    quantitative Polymerase Chain Reaction assays quantify the DNATechnique requires prior knowledge of organisms genomeProbes and primers complement and bind to target DNA *

  • Methods Organism Quantification *Collect SampleExtract and Purify DNAQuantify the DNA of important anaerobes

  • Releasing DNAThe key to good analysis is the total release of DNA from the cell. Use glass beads to beat cells in order to break the cell wall and membrane.DNA Needs to be released by beating with glass beads*Olson, UCI, CEE 263, 2008

  • Flowcell Basic PrinciplesFirst Cell Measures Total Gas, Second Cell Measures MethaneGlass scrubber with 2 M NaOH to remove CO2. , Bromocresol Green Indicator, color transition, clear to green at pH 12

  • R1R2 - 5 V + Two Resistors in SeriesR1: 12k , R2:Cd_S(1-50)k

    15 mC LED & 200 R Gas inGas OutFLOW-CELL - + Volts

  • Plumping and Circuit Diagram, 6 ReactorsPhysical connections to Match Software OrganizationR1R3R4R2R5R612345678USB DevicePC

    be monitored to see effectsThis is a commonly accepted simplification of the anaerobic digestinon processs;

    Hydrolysis, acetogenesis, methanogeneis

    Our analysis was largely focused on the last step of the process, methanogenesis.Good Morning ladies and gentlemen

    My name is Zachary Scott.

    I am a PHD at the University of California IrvineToday I will be presenting the results of a collaborative study by UC Irvine and Brown and Caldwell to improve the performance of a Anaerobic Plug-Flow Manure digester in Chino California, on behalf of the Inland Empire Utilities AgencyGood Morning ladies and gentlemen

    My name is Zachary Scott.

    I am a PHD at the University of California IrvineToday I will be presenting the results of a collaborative study by UC Irvine and Brown and Caldwell to improve the performance of a Anaerobic Plug-Flow Manure digester in Chino California, on behalf of the Inland Empire Utilities AgencyGood Morning ladies and gentlemen

    My name is Zachary Scott.

    I am a PHD at the University of California IrvineToday I will be presenting the results of a collaborative study by UC Irvine and Brown and Caldwell to improve the performance of a Anaerobic Plug-Flow Manure digester in Chino California, on behalf of the Inland Empire Utilities Agencygravity-filled the chamber*

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