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The Queensland Node of Proteomics Australia The Queensland Node of Proteomics Australia offers high throughput quantitative proteomics services to all life science researchers. Services are offered through a consortium of Australian universities and research institutes with world class facilities. Services offered Sample Preparation Automated Sample preparation Protein Identification By Data Dependant Analysis Protein quantification By Data Dependent Analysis By Data Independent Analysis By Isobaric Tag Analysis Protein modification Analysis By phosphorylation, Γ carboxylation Data analysis Data processing workflows Our New ThermoFisher Q-Exactive HF-X offers a new standard in sensitivity, performance and productivity. Contact +61 7 334 63126 [email protected] aibn.uq.edu.au/bpa

The Queensland Node of Proteomics Australia

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Page 1: The Queensland Node of Proteomics Australia

The Queensland Node of Proteomics Australia

The Queensland Node of Proteomics Australia offers high throughput quantitative proteomics services to all life science researchers. Services are offered through a consortium of Australian universities and research institutes with world class facilities.

Services offered

Sample PreparationAutomated Sample preparation

Protein IdentificationBy Data Dependant Analysis

Protein quantificationBy Data Dependent Analysis

By Data Independent Analysis

By Isobaric Tag Analysis

Protein modification AnalysisBy phosphorylation, Γ carboxylation

Data analysisData processing workflows

Our New ThermoFisher Q-Exactive HF-X offers a new standard in sensitivity, performance and productivity.

Contact+61 7 334 63126 [email protected]

aibn.uq.edu.au/bpa

Page 2: The Queensland Node of Proteomics Australia

Our node is part of a national consortium initiative, comprising the Adelaide Proteomics Centre (University of Adelaide), The Monoclonal Antibody Technology Facility (Monash University), Monash Biomedical Proteomics Facility (Monash University), the Australian Proteome Analysis Facility (Macquarie University) and Proteomics International.

Cofficient of variation of protein quantification

Cofficient of variation of peptide group quantification

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0100ng 200ng 400ng 800ng

100ng 200ng 400ng 800ng

Label-free quantification of a HeLa sample

The Queensland node of Proteomics Australia specialises in high throughput quantitative proteomics, particularly label-free proteomics for systems biology and biotechnology applications. Label-free quantification is the quantification of MS peptide signals by LC/MS/MS data without an isotopic label. For example, we can quantify up to 8700 proteins in 200ng of a HeLa digest, from which more than 3400 have a median CV of less than 10% using 200 ng of HeLa digest (equivalent to ~1000 cells).

TotalCV <30%CV <20%CV <10%

Amount of HeLa digest standard injected

Amount of HeLa digest standard injected