TECHNICAL APPENDIX••••

Solvent CompatibilityNomogram for Computing Relative Centrifugal ForcesEuroClone PRIMO® Tips Compatibility ChartEuroClone PREMIER Filter Tips Compatibility ChartCompatibility Chart: Plates & SealsList of available Rotors

•

•

142

18 Technical Appendix

Plastic Chemical and Condition Compatibility Chart This chart is only provided as a general guide and not an unqualified guarantee of

chemical compatibility. Because so many factors can affect the chemical resistance of a given product,

we recommend that you test under your own conditions.

GENERAL CONDITIONS INFORMATION

Abbreviation Chemical Designation

Tem

p ºC

(Max

)

Tem

p ºC

(Min

)

Auo

tcla

vabl

e

Mic

row

avab

le

Dry

Hea

t

Gas

Gam

ma

Dis

infe

ctan

ts

LDPE Low Density Polyethylene 100 - 80 NO YES NO YES YES YESHDPE High Density Polyethylene 120 - 100 NO YES NO YES YES YES PC Polycarbonate 135 - 135 YES YES NO YES YES YESPP Polypropylene 135 - 90 YES YES NO YES NO YESPS Polystyrene 70 - 40 NO YES NO YES YES YES

zSolvent LDPE HDPE PC PP PS

Acetaldehyde C B C C DAcetic Anhydride D D D B DAcetone C C D A DAcid, Hydroflouric A A D B DAcid, Trifluoroacetic D C D D DAcid, Acetic Dilute 50% A A B A BAcid, Hydrochloric 37% A A D D CAcid, Nitric C B B D CAcid, Sulfuric B A C C CAlcohol, Ethyl B A B B BAlcohol, Isobutyl A A B A BAlcohol, Methyl A A B A CAlcohol, n-Butyl A A C A BAlcohol, Propyl A A D A AAmmonium Hydroxide B A - B BAniline B B B B DAqua Regia D C D D DBenzaldehyde B A C A DBenzene D D D B DCarbon Tetrachloride B C D B DCaustic Soda (NaOH) B A D A AChlorobenzene D C D D DChloroform C C D B DCyclohexane C C D C DEsters B B D B DEther D C C D DEther, Diethyl A D D D DEther, Isopropyl A A A A AEthtyl, Methyl B D D D ATexane B C C D AHydrazine - D D D AHydrogen Peroxide A A A B AMethylene Chloride C D C D APetroleum Ether A A - B APhenol D D D C ASodium Hydroxide A D A A ATetrahydrofuran B D C D AToluene B D C D ATrichloroethylene C D D D ATrimethylpentane C D C D AXylene C D C D A

A No Effect, excellent compatibility

B Minor Effect, good compatibility

C Moderate Effect, fair compatibility

D Severe Effect, not recommended

- No data available

Technical Appendix 18

143

Nomogram for Computing Relative Centrifugal Forces

Sample CalculationThe relative centrifugal force (RCF) at a radial distance of 8cm from the center of centrifuge spindle when operating at a speed of 2500 r.p.m., may be determined by placing a straight edge on the nomogram connecting the 8cm point on the Radius from Center of Spindle Scale (A), with the 2500 r.p.m. point on the Speed Scale (B). The point of intersection on the Relative Centrifugal Force Scale (C), or 550g is the relative centrifugal force (550 x 32 = 17600 psi).

If the desired RCF is known, the speed of rotation for a given radius may be found by connecting the two known points and reading the Speed Scale at the intersection.

Technical Appendix 18

145

VOLUMES

ECT0

110

0,1-

10 u

l

ECTL

110

0,1-

10 u

l

ECT0

510

1-10

ul

ECT0

030X

F2-

20 u

l

ECT0

100X

F2-

100

ul

ECTY

200

2-20

0 u

l

ECTN

200

2-20

0 u

l

ECT0

200X

F2-

200

ul

ECTG

200R

C2-

200

ul

ECT0

300

20-3

00 u

l

ECT0

300X

F20

-300

ul

ECTB

1000

EC

TN10

0010

0-10

00 u

l

ECTB

1005

101-

1000

ul

ECT5

000

1-5

ml

ECT1

0000

2-10

ml

BIOHIT proline 1 ch0,1-2,5ul0,5-10ul X X X X2-20ul X X

10-100ul X X20-200ul X X X X X

100-1000ul X XBIOHIT proline plus 1ch

0,1-3 ul 0,5-10ul2-20ul X X

10-100ul X X X20-200ul X X X

100-1000ul X XBIOHIT mine plus 1ch

0,1-3 ul 0,5-10ul X X X2-20ul X X

10-100ul X X X20-200ul X X X

100-1000ul X XBRAND transferpette

0,5-10ul X X X2-20ul X X

10-100ul X X20-200ul X X

100-1000ul X XEPPENDORF research

0,5-10ul X X X2-20ul X

10-100ul X X X X20-200ul X X X X X X

100-1000ul X XEPPENDORF reference

0,5-10ul X2-20ul X

10-100ul X X X X50-200ul X X X X X

100-1000ul X XGILSON pipetman

0,2-2ul X X1-10ul X X2-20ul X X X

20-100ul X X X X X20-200ul X X X X

200-1000ul X5 ml X10ml X

THERMO finpipette0,5-10ul X X X2-20ul X

10-100ul X X X X X X20-200ul X X X X X

100-1000ul X5ml10ml X

EuroClone PRIMO® Tips Compatibility Chart

146

18 Technical Appendix

EC10010FTS10ul

EC10020FTS20ul

EC10100FTS100ul

EC10200FTS200ul

EC11000FTS1000ul

EPPENDORF

Reference

0,5-10 µl •

2-20 µl yellow •

10-100 µl •50-200 µl •100-1000 µl •

Research

0,5-10 µl • 2-20 µl • 10-100 µl • 20-200 µl • 100-1000 µl •

Research 8ch 0,5-10 µl • Research plus 20-200 µl •

GILSON

Ultra

0,2-2 µl • *1-10 µl • *2-20 µl no20-100 µl • 20-200 µl • 200-1000 µl •

Pipetman P 0,2-2 µl •

Pipetman Neo

1-10 µl • 2-20 µl no10/20-100 µl • 20/50-200 µl • 100/200-1000 µl •

Pipetman Neo 8ch 20-200 µl •

BRAND

Transferpette S

0,5-10 µl • 2-20 µl • 10-100 µl • 20-200 µl • 100-1000 µl •

THERMO

Focus short0,3-3 µl • 0,5-5 µl • 100-1000 µl •

Focus 8ch 1-10 µl • Focus 12ch 1-10 µl • Focus 12ch 5-50 µl noFinpipette Digital 0,2-2 µl • Finpipette Digital 0,5-10 µl • Finpipette Digital 10-100 µl • Finpipette Digital 40-200 µl • Finpipette Digital 100-1000 µl • Finpipette Digital 200-1000 µl • Finpipette Digital 8ch 0,5-10 µl • Finpipette Digital 8ch 5-50 µl noThermo F1 0,2-2 µl • Thermo F1 0,5-5 µl • Thermo F1 1-10 µl • Thermo F1 2-20 µl • Thermo F1 10-100 µl • • Thermo F1 20-200 µl • Thermo F1 100-1000 µl • Thermo F1 8ch 1-10 µl • Thermo F1 8ch 5-50 µl • • Thermo F1 8ch 10-100 µl • • Thermo F2 0,2-2 µl • Thermo F2 0,5-5 µl • Thermo F2 1-10 µl • Thermo F2 2-20 µl • Thermo F2 10-100 µl • • Thermo F2 20-200 µl • Thermo F3 100-1000 µl • Thermo F2 8ch 1-10 µl • Thermo F2 8ch 5-50 µl • • Thermo F2 8ch 10-100 µl • •

EuroClone PREMIER Filter Tips Compatibility Chart

Technical Appendix 18

147

EC10010FTS10ul

EC10020FTS20ul

EC10100FTS100ul

EC10200FTS200ul

EC11000FTS1000ul

BIOHIT

Elettronic Pipettes

eLINE 1ch BH730021 0,2-10 µl • eLINE 1ch BH730041 5-120 µl • eLINE 1ch BH730081 50-1000 µl • eLINE 8ch BH730321 0,2-10 µl • eLINE 8ch BH730341 5-120 µl • eLINE 12ch BH730421 0,2-10 µl • eLINE 12ch BH730441 5-120 µl • Proline 1ch BH710520 0,2-10 µl • Proline 1ch BH710010 5-100 µl • Proline 1ch BH710020 50-1000 µl • Proline 8ch BH710200 0,2-10 µl • Proline 8ch BH710210 5-100 µl • • Proline 12ch BH710300 0,2-10 µl • Proline 12ch BH710310 5-100 µl • • ePET 1ch BH710521ET 0,2-10 µl • ePET 1ch BH710011ET 5-100 µl • ePET 1ch BH710021ET 50-1000 µl • ePET 8ch BH710201ET 0,2-10 µl • ePET 8ch BH710211ET 5-100 µl • • ePET 12ch BH710301ET 0,2-10 µl • ePET 12ch BH710311ET 5-100 µl • •

Mechanical Pipettes Adjsustable Volume

mLINE 1ch BH725010 0,1-3 µl • mLINE 1ch BH725020 0,5-10 µl • mLINE 1ch BH725030 2-20 µl • mLINE 1ch BH725050 10-100 µl • mLINE 1ch BH725060 20-200 µl • mLINE 1ch BH725070 100-1000 µl • mLINE 8ch BH725120 0,5-10 µl • mLINE 8ch BH725130 5-100 µl • mLINE 12ch BH725220 0,5-10 µl • mLINE 12ch BH725230 5-100 µl • Proline P 1ch BH728010 0,1-3 µl • Proline P 1ch BH728020 0,5-10 µl • Proline P 1ch BH728030 2-20 µl • Proline P 1ch BH728050 10-100 µl • Proline P 1ch BH728060 20-200 µl • Proline P 1ch BH728070 100-1000 µl • Proline P 8ch BH728120 0,5-10 µl • Proline P 8ch BH728130 10-100 µl • Proline P 12ch BH728220 0,5-10 µl • Proline P 12ch BH728230 10-100 µl • Proline 1ch BH720005 0,1-2,5 µl • Proline 1ch BH720000 0,5-10 µl • • Proline 1ch BH720080 2-20 µl • • Proline 1ch BH720020 5-50 µl • Proline 1ch BH720050 10-100 µl • • Proline 1ch BH720070 20-200 µl • • Proline 1ch BH720060 100-1000 µl • Proline 8ch BH720210 0,5-10 µl • Proline 8ch BH720220 5-50 µl • • Proline 12ch BH720310 0,5-10 µl • Proline 12ch BH720320 5-50 µl • •

Mechanical Pipettes Fixed Volume

Proline P & Proline 5 µl • • Proline P & Proline 10 µl • • Proline P & Proline 20 µl • • Proline P & Proline 25 µl • Proline P & Proline 50 µl • Proline P & Proline 100 µl • • Proline P & Proline 200 µl • Proline P & Proline 250 µl • Proline P & Proline 500 µl • Proline P & Proline 1000 µl •

The symbol indicates the fitting and the tip ejection of our Premier filter tips as follows:• Fitting, (no) No fitting, * Tip ejection does not workThe compatibility chart above is given for guidance. EuroClone is not responsible for any technical modifications or changes made to the pipettes listed.

148

18 Technical Appendix

VOLUMES

ECT0

110

0,1-

10 u

l

ECTL

110

0,1-

10 u

l

ECT0

510

1-10

ul

ECT0

030X

F2-

20 u

l

ECT0

100X

F2-

100

ul

ECTY

200

2-20

0 u

l

ECTN

200

2-20

0 u

l

ECT0

200X

F2-

200

ul

ECTG

200R

C2-

200

ul

ECT0

300

20-3

00 u

l

ECT0

300X

F20

-300

ul

ECTB

1000

EC

TN10

0010

0-10

00 u

l

ECTB

1005

101-

1000

ul

ECT5

000

1-5

ml

ECT1

0000

2-10

ml

BIOHIT proline 1 ch0,1-2,5ul0,5-10ul X X X X2-20ul X X

10-100ul X X20-200ul X X X X X

100-1000ul X XBIOHIT proline plus 1ch

0,1-3 ul 0,5-10ul2-20ul X X

10-100ul X X X20-200ul X X X

100-1000ul X XBIOHIT mine plus 1ch

0,1-3 ul 0,5-10ul X X X2-20ul X X

10-100ul X X X20-200ul X X X

100-1000ul X XBRAND transferpette

0,5-10ul X X X2-20ul X X

10-100ul X X20-200ul X X

100-1000ul X XEPPENDORF research

0,5-10ul X X X2-20ul X

10-100ul X X X X20-200ul X X X X X X

100-1000ul X XEPPENDORF reference

0,5-10ul X2-20ul X

10-100ul X X X X50-200ul X X X X X

100-1000ul X XGILSON pipetman

0,2-2ul X X1-10ul X X2-20ul X X X

20-100ul X X X X X20-200ul X X X X

200-1000ul X5 ml X10ml X

THERMO finpipette0,5-10ul X X X2-20ul X

10-100ul X X X X X X20-200ul X X X X X

100-1000ul X5ml10ml X

COMPATIBILITY CHART: PLATES & SEALS

150

18 Technical AppendixPlate Compatibility Table

1 Mx4000® instruments made after 2003 are compatible

2 For MegaBase purchased after July 2000

3 PCR plate will need to be cut into 24-well strips

96well skirted

ECPCR0740

96well non-skirted

ECPCR0750

96well semi-skirtedECPCR0760

96well non-skirted,

std profileECPCR0710

96well with upstandECPCR0730

96well skirted

ECPCR0960

96well semi-

skirted (cut A12)

ECPCR0770

96well semi-

skirted (cut H1)

ECPCR0900

96well FastPlate

ECPCR0910C

96well 480ECPCR0950/0951

384well plateECPCR0384

384well 480ECPCR0380/0381

8-tube stripECPCR0208

96well non-skirted,

low profileECPCR0720

AB

i

Ver

iti

38

4w

ell

Blo

ck

Ver

iti

0.1

ml

96

wel

l B

lock

Ver

iti

0.2

ml

96

wel

l B

lock

Gen

eAm

p® 2

40

0

G

eneA

mp

® 2

70

0/2

72

0

Gen

eAm

p® 9

60

0

Gen

eAm

p® 9

70

0

G

eneA

mp

® 9

80

0 F

AS

T B

lock

Bio

er

XP

Cycl

er

Gen

e P

ro

L

ittl

e G

eniu

s

Lif

e E

xpre

ss

G

eneQ

Lif

ePro

Bio

met

ra

U

no

Un

o I

I

Tper

son

al

Cycl

er

T1

Th

erm

ocy

cler

T

3 T

her

mocy

cler

TG

radie

nt

TR

obot

Bio

-Rad

G

enec

ycl

er

iC

ycl

er™

My

Cycl

er

C10

00

S

100

0

B

io-R

ad-M

J R

esea

rch

Min

i G

radie

nt

Per

son

al

PT

C10

0™ (

96

wel

l blo

ck o

nly

)

PT

C2

00

(D

NA

En

gin

e™)

P

TC

22

0/2

21

(DN

A D

yad

™)

P

TC

22

5 (

DN

A T

etra

d™)

Corb

ett

Res

earc

h (

Qia

gen

)

P

alm

Cycl

er

P

alm

Cycl

er 3

84

Eppen

dorf

Mast

erC

ycl

er®

Mast

erC

ycl

er® G

radie

nt

Mast

erC

ycl

er® E

P G

radie

nt

M

38

4

E

rico

mp

Sin

gle

Blo

ck S

yst

em

Tw

inB

lock

Syst

em

D

elta

cycl

er I

Del

tacy

cler

II

Pow

er B

lock

I

E

sco

Sw

ift

Euro

Clo

ne

Bio

er

Eu

roC

ycl

er F

lex

Eu

roC

ycl

er M

ini

Eu

roC

ycl

er 9

6 G

radie

nt

Euro

Clo

ne P

eqla

b

Eu

roC

lon

epeq

Sta

r 9

6 U

niv

ersa

l

E

uro

Clo

ne

peq

Sta

r 9

6 G

radie

nt

Fle

xi

Gen

e

G

eniu

s

G

-Sto

rm

G

S1,

GS

2,

GS

3

G

S4

GS

X

M

WG

Pri

mu

s 9

6

P

rim

us

38

4

T

he

Q-L

ifec

ycl

er

P

eqla

b

peq

STA

R 9

6

peq

STA

R 3

84

Sen

soQ

ues

t

labcy

cler

Str

atag

ene

Robocy

cler

Gra

die

nt

Cycl

er

Tak

ara

TP

24

0

T

P3

00

0

Te

chne

TC

412

/512

Tou

chgen

e

To

uch

gen

e G

radie

nt

Cycl

ogen

e

F

lex

igen

e

G

eniu

s

G

eniu

s Q

uad

Ther

mo

PC

R X

pre

ss/P

x2

/Px

E

P

CR

Spri

nt

Mu

ltiB

lock

Syst

em &

MB

S

To

uch

dow

n

O

mn

igen

e

O

mn

i-E

AB

i

7

00

0/7

30

0

7

50

0/7

70

0/7

90

0

FAS

T 7

50

0/7

90

0/7

90

0H

T

79

00

HT

S

tepO

ne

Plu

s™

Vii

A™

Bio

er

L

ine-

Gen

e 9

60

0

B

io-R

ad

iC

ycl

er™ I

Q

iQ

™ 4

, iQ

™ 5

My

iQ™

CF

X9

6

CF

X3

84

Bio

-Rad

-MJ

Res

earc

h

O

pti

con

™

Opti

con

2™

Ch

rom

o4

™

Eppen

dorf

Mast

ercy

cler

™ e

p r

eal

ple

x

R

och

e

LC

48

0

S

trat

agen

e

M

x3

00

0P

™

Mx

30

05

P™

MX

40

00

™

Tech

ne

Qu

an

tica

AB

i

3

100

Gen

etic

An

aly

ser

313

0 G

enet

ic A

naly

ser

310

Gen

etic

An

aly

ser

35

00

Gen

etic

An

aly

ser

35

00

3

50

0X

L

37

00

DN

A A

naly

ser

3

73

0/3

73

0X

L D

NA

An

aly

ser

Am

ersh

am

M

egaB

AC

E™ 5

00

Meg

aB

AC

E™ 1

00

0 m

ark

2

Meg

aB

AC

E™ 4

00

0

B

eckm

an

C

EQ

MJ

Res

earc

h

B

ase

Sta

tion

™

Tra

nsg

enom

ic

W

ave

3 3 3

Standard Cyclers

technical_appendix.indd 1 25/01/12 10:12

Technical Appendix 18

151

AB

i

Ver

iti

38

4w

ell

Blo

ck

Ver

iti

0.1

ml

96

wel

l B

lock

Ver

iti

0.2

ml

96

wel

l B

lock

Gen

eAm

p® 2

40

0

G

eneA

mp

® 2

70

0/2

72

0

Gen

eAm

p® 9

60

0

Gen

eAm

p® 9

70

0

G

eneA

mp

® 9

80

0 F

AS

T B

lock

Bio

er

XP

Cycl

er

Gen

e P

ro

L

ittl

e G

eniu

s

Lif

e E

xpre

ss

G

eneQ

Lif

ePro

Bio

met

ra

U

no

Un

o I

I

Tper

son

al

Cycl

er

T1

Th

erm

ocy

cler

T

3 T

her

mocy

cler

TG

radie

nt

TR

obot

Bio

-Rad

G

enec

ycl

er

iC

ycl

er™

My

Cycl

er

C10

00

S

100

0

B

io-R

ad-M

J R

esea

rch

Min

i G

radie

nt

Per

son

al

PT

C10

0™ (

96

wel

l blo

ck o

nly

)

PT

C2

00

(D

NA

En

gin

e™)

P

TC

22

0/2

21

(DN

A D

yad

™)

P

TC

22

5 (

DN

A T

etra

d™)

Corb

ett

Res

earc

h (

Qia

gen

)

P

alm

Cycl

er

P

alm

Cycl

er 3

84

Eppen

dorf

Mast

erC

ycl

er®

Mast

erC

ycl

er® G

radie

nt

Mast

erC

ycl

er® E

P G

radie

nt

M

38

4

E

rico

mp

Sin

gle

Blo

ck S

yst

em

Tw

inB

lock

Syst

em

D

elta

cycl

er I

Del

tacy

cler

II

Pow

er B

lock

I

E

sco

Sw

ift

Euro

Clo

ne

Bio

er

Eu

roC

ycl

er F

lex

Eu

roC

ycl

er M

ini

Eu

roC

ycl

er 9

6 G

radie

nt

Euro

Clo

ne P

eqla

b

Eu

roC

lon

epeq

Sta

r 9

6 U

niv

ersa

l

E

uro

Clo

ne

peq

Sta

r 9

6 G

radie

nt

Fle

xi

Gen

e

G

eniu

s

G

-Sto

rm

G

S1,

GS

2,

GS

3

G

S4

GS

X

M

WG

Pri

mu

s 9

6

P

rim

us

38

4

T

he

Q-L

ifec

ycl

er

P

eqla

b

peq

STA

R 9

6

peq

STA

R 3

84

Sen

soQ

ues

t

labcy

cler

Str

atag

ene

Robocy

cler

Gra

die

nt

Cycl

er

Tak

ara

TP

24

0

T

P3

00

0

Te

chne

TC

412

/512

Tou

chgen

e

To

uch

gen

e G

radie

nt

Cycl

ogen

e

F

lex

igen

e

G

eniu

s

G

eniu

s Q

uad

Ther

mo

PC

R X

pre

ss/P

x2

/Px

E

P

CR

Spri

nt

Mu

ltiB

lock

Syst

em &

MB

S

To

uch

dow

n

O

mn

igen

e

O

mn

i-E

AB

i

7

00

0/7

30

0

7

50

0/7

70

0/7

90

0

FAS

T 7

50

0/7

90

0/7

90

0H

T

79

00

HT

S

tepO

ne

Plu

s™

Vii

A™

Bio

er

L

ine-

Gen

e 9

60

0

B

io-R

ad

iC

ycl

er™ I

Q

iQ

™ 4

, iQ

™ 5

My

iQ™

CF

X9

6

CF

X3

84

Bio

-Rad

-MJ

Res

earc

h

O

pti

con

™

Opti

con

2™

Ch

rom

o4

™

Eppen

dorf

Mast

ercy

cler

™ e

p r

eal

ple

x

R

och

e

LC

48

0

S

trat

agen

e

M

x3

00

0P

™

Mx

30

05

P™

MX

40

00

™

Tech

ne

Qu

an

tica

AB

i

3

100

Gen

etic

An

aly

ser

313

0 G

enet

ic A

naly

ser

310

Gen

etic

An

aly

ser

35

00

Gen

etic

An

aly

ser

35

00

3

50

0X

L

37

00

DN

A A

naly

ser

3

73

0/3

73

0X

L D

NA

An

aly

ser

Am

ersh

am

M

egaB

AC

E™ 5

00

Meg

aB

AC

E™ 1

00

0 m

ark

2

Meg

aB

AC

E™ 4

00

0

B

eckm

an

C

EQ

MJ

Res

earc

h

B

ase

Sta

tion

™

Tra

nsg

enom

ic

W

ave

2

11

2

qPCR CyclersStandard Cyclers Sequencers

technical_appendix.indd 2 25/01/12 10:12

152

18 Technical Appendix

Adhesive Sealing Films & Foils

Description Primo® Adhesive PCR seal Primo® Q-Stick for

qPCR Primo® qPCR seal Primo® PCR foil seal

Primo® piercable PCR film

Primo® solvent resistant seal

Primo® microplate seal

Cat. ECPCR0500 ECPCR0565 ECPCR0560 ECPCR0550 ECPCR0566 ECPCR0512 ECPCR0510

Type Adhesive Adhesive Adhesive Adhesive Adhesive Adhesive Adhesive

Application PCR qPCR, fluorescence. 96-well microplates

only

qPCR & other fluorescent applications

PCR & sample storage Sample application or retrieval. 96-well

plates only.

Microplate sealing containing solvents

incl. DMSO

Aqueous sample storage

Special Properties Good optical clarity.Discreet optical

windows for 96-well plates

Good optical clarityIrregular tearing when

pierced prevents formation of vacuum

Pre-cut with cross which reduces tip

or probe becoming clogged

High solvent resistanceMedium strength transparent seal

Min Temp -20°C -20°C -4°C -40°C -20°C -20°C -20°C Max Temp 110°C 110°C 110°C 120°C 110°C 80°C 80°C Sterile No No No No No No No Pierceable No No No Yes Yes No No Peelable Yes Yes Yes Yes Yes Yes Yes RNase/DNase free Yes Yes Yes Yes Yes No Yes

Measurements (mm)* Total = 137, Adhesive = 117 (no tear strips) Adhesive = 119

(inside tear strips) Adhesive = 116 (inside

tear strips)Adhesive = 118 (plus

2 x 8 mm tabs)Total = 134, Adhesive = 113 (no tear strips)

Adhesive = 122 Total = 131 (no tear

strips) All Adhesive Sealing Films & Foils have width of 80mm

Heat Sealing Films & Foils

DescriptionPrimo® Transparent

Seal IPrimo® Transparent

Seal II

Primo® Transparent Seal For ABI 3730

Sequencer

Primo® Peel-Seal heat sealing

Primo® DMSO resistant seal

Primo® Pierceable seal heat sealing

Primo® Foil-Seal peelable heat

sealing

Primo® Thermalheat sealing

Cat.

Roll 78mm 1 ECPCR0540 - - ECPCR0520 ECPCR0585 - - -Roll 115mm 2 ECPCR0542 -Pack of 100 sheets (125mm x 78mm)

ECPCR0541 ECPCR0575 ECPCR0581 + ECPCR0521 ECPCR0587# ECPCR0531 ECPCR0536 ECPCR0591

Type Heat Heat Heat Heat Heat Heat Heat Heat

ApplicationqPCR, short term

compound storage.

PCR (esp. water bath), qPCR.Storage &

disposal of hazardous materials.

For use with ABI 3730 Sequencer.

Low temperature, compound storage,

short term room temperature

compound storage(<5 days), PCR.

Low temperature /room temperature compound storage

with DMSO^ & other organic solvents.

PCR, compound storage, sample

shipping

Low temperature compound storage,

short-term room temperature

compound storage, PCR.

Low temperature transportation &

storage. PCR, esp. water bath cyclers. Storage of organic solvents, acids &

alkalines.

Special PropertiesGood optical clarity. Moderate solvent

resistance

Good optical clarity & resistant to DMSO.

Good optical clarity,some solvent

resistance.

Can be peeled directly from -80°C freezer. Moderate resistance to solvents at room

temperature.

Can be peeled directly from -80°C freezer. High resistance to solvents even at

elevated temperatures.

Easily pierceable. Resistant to DMSO.

Re-sealable with another Pierce Seal. Color print identifies non-sealing surface.

Re-sealable with another Foil Seal.

Colour print identifies non-sealing surface.

Very strong seal with PP. Resistant to DMSO

and other solvents

Min Temp -80°C 80°C 80°C 80°C 80°C -20°C -20°C -80°C

Max Temp80°C (or 110°C with

pressurised PCR heated lid)

110°C80°C (or 110°C with

pressurised PCR heated lid)

110°C 80°C 120°C 110°C 110°C

Sterile No No No No No No* No* No Pierceable No No Yes No No Yes Yes No Peelable Yes No No Yes Yes No Yes YesRNase/DNase free Yes Yes Yes Yes No Yes Yes Yes Material Laminate Laminate Polymer Laminate Laminate Foil Foil Laminate Seals to PP, PE, PS, COC PP PP, PE, PS, COC PP PP PP, PS PP, PS PP PS

Roll length mm (78mm width)

500 610 610 600 500 610 610 500

Roll length mm (115mm width)

350 500 500 500 500 500 500 300

^ 100% DMSO can be stored at room temperature for 12 months with no deterioration of the seal quality * Available as sterile on request + Roll of 1000 Perforated Sheets # Supplied on a perforated roll 1 Compatible with Thermo Fisher ALPSs 300™ / ALPS3000™ / KBiosystems Chameleon™ / KBioscience FexiSeal & Remp/Tecan Plate Sealer 2 Compatible with Agilent (Velocity 11 )PlateLoc® PP: Polypropylene / PS: Polystyrene / COC: Cyclic Olefin Copolymer / PE: Polythene NB: Rolls are also available on 150mm core for Remp/Tecan sealers. Please enquire.

Technical Appendix 18

153

Adhesive Sealing Films & Foils

Description Primo® Adhesive PCR seal Primo® Q-Stick for

qPCR Primo® qPCR seal Primo® PCR foil seal

Primo® piercable PCR film

Primo® solvent resistant seal

Primo® microplate seal

Cat. ECPCR0500 ECPCR0565 ECPCR0560 ECPCR0550 ECPCR0566 ECPCR0512 ECPCR0510

Type Adhesive Adhesive Adhesive Adhesive Adhesive Adhesive Adhesive

Application PCR qPCR, fluorescence. 96-well microplates

only

qPCR & other fluorescent applications

PCR & sample storage Sample application or retrieval. 96-well

plates only.

Microplate sealing containing solvents

incl. DMSO

Aqueous sample storage

Special Properties Good optical clarity.Discreet optical

windows for 96-well plates

Good optical clarityIrregular tearing when

pierced prevents formation of vacuum

Pre-cut with cross which reduces tip

or probe becoming clogged

High solvent resistanceMedium strength transparent seal

Min Temp -20°C -20°C -4°C -40°C -20°C -20°C -20°C Max Temp 110°C 110°C 110°C 120°C 110°C 80°C 80°C Sterile No No No No No No No Pierceable No No No Yes Yes No No Peelable Yes Yes Yes Yes Yes Yes Yes RNase/DNase free Yes Yes Yes Yes Yes No Yes

Measurements (mm)* Total = 137, Adhesive = 117 (no tear strips) Adhesive = 119

(inside tear strips) Adhesive = 116 (inside

tear strips)Adhesive = 118 (plus

2 x 8 mm tabs)Total = 134, Adhesive = 113 (no tear strips)

Adhesive = 122 Total = 131 (no tear

strips) All Adhesive Sealing Films & Foils have width of 80mm

Heat Sealing Films & Foils

DescriptionPrimo® Transparent

Seal IPrimo® Transparent

Seal II

Primo® Transparent Seal For ABI 3730

Sequencer

Primo® Peel-Seal heat sealing

Primo® DMSO resistant seal

Primo® Pierceable seal heat sealing

Primo® Foil-Seal peelable heat

sealing

Primo® Thermalheat sealing

Cat.

Roll 78mm 1 ECPCR0540 - - ECPCR0520 ECPCR0585 - - -Roll 115mm 2 ECPCR0542 -Pack of 100 sheets (125mm x 78mm)

ECPCR0541 ECPCR0575 ECPCR0581 + ECPCR0521 ECPCR0587# ECPCR0531 ECPCR0536 ECPCR0591

Type Heat Heat Heat Heat Heat Heat Heat Heat

ApplicationqPCR, short term

compound storage.

PCR (esp. water bath), qPCR.Storage &

disposal of hazardous materials.

For use with ABI 3730 Sequencer.

Low temperature, compound storage,

short term room temperature

compound storage(<5 days), PCR.

Low temperature /room temperature compound storage

with DMSO^ & other organic solvents.

PCR, compound storage, sample

shipping

Low temperature compound storage,

short-term room temperature

compound storage, PCR.

Low temperature transportation &

storage. PCR, esp. water bath cyclers. Storage of organic solvents, acids &

alkalines.

Special PropertiesGood optical clarity. Moderate solvent

resistance

Good optical clarity & resistant to DMSO.

Good optical clarity,some solvent

resistance.

Can be peeled directly from -80°C freezer. Moderate resistance to solvents at room

temperature.

Can be peeled directly from -80°C freezer. High resistance to solvents even at

elevated temperatures.

Easily pierceable. Resistant to DMSO.

Re-sealable with another Pierce Seal. Color print identifies non-sealing surface.

Re-sealable with another Foil Seal.

Colour print identifies non-sealing surface.

Very strong seal with PP. Resistant to DMSO

and other solvents

Min Temp -80°C 80°C 80°C 80°C 80°C -20°C -20°C -80°C

Max Temp80°C (or 110°C with

pressurised PCR heated lid)

110°C80°C (or 110°C with

pressurised PCR heated lid)

110°C 80°C 120°C 110°C 110°C

Sterile No No No No No No* No* No Pierceable No No Yes No No Yes Yes No Peelable Yes No No Yes Yes No Yes YesRNase/DNase free Yes Yes Yes Yes No Yes Yes Yes Material Laminate Laminate Polymer Laminate Laminate Foil Foil Laminate Seals to PP, PE, PS, COC PP PP, PE, PS, COC PP PP PP, PS PP, PS PP PS

Roll length mm (78mm width)

500 610 610 600 500 610 610 500

Roll length mm (115mm width)

350 500 500 500 500 500 500 300

^ 100% DMSO can be stored at room temperature for 12 months with no deterioration of the seal quality * Available as sterile on request + Roll of 1000 Perforated Sheets # Supplied on a perforated roll 1 Compatible with Thermo Fisher ALPSs 300™ / ALPS3000™ / KBiosystems Chameleon™ / KBioscience FexiSeal & Remp/Tecan Plate Sealer 2 Compatible with Agilent (Velocity 11 )PlateLoc® PP: Polypropylene / PS: Polystyrene / COC: Cyclic Olefin Copolymer / PE: Polythene NB: Rolls are also available on 150mm core for Remp/Tecan sealers. Please enquire.

154

18 Technical Appendix

CODE PHOTO ROTOR TYPE MATERIAL MAX. SPEED (RPM) MAX. RCF (XG) VOLUME (ml) DESCRIPTION DIMENSIONS (ø x mm) QUANTITY ADAPTERS AUTOCLAVAbLE ROTOR LID CENTRIFUGE MODEL

EFA18b Fixed angle Aluminium 18000 24990 1.5 / 2.0

0:05

0:02

Microtube

PCR Microtube

PCR Microtube

10.5 x 41

8 x 32

6 x 24

18 x 1.5 / 2.0

18 x 0.5 ml

18 x 0.2 ml

N.A.

EFA1505

EFA1502

121° C Aluminium Only for Speed Master 18R

EFA15A Fixed angle Aluminium 15000 21500 1.5 / 2.0

0:05

0:02

Microtube

PCR Microtube

PCR Microtube

10.5 x 41

8 x 32

6 x 24

24 x 1.5 / 2.0 ml

24 x 0.5 ml

25 x 0.2 ml

N.A.

EFA1505

EFA1502

121° C Aluminium Speed Master 14/ 14R/ 18R

EFA12A Fixed angle Aluminium 12000 14100 0,2 PCR Microtube/strip 6 x 24 48 x 0,2 ml N.A. 121° C N.A. Speed Master 14/ 14R/ 18R

EFA18A Fixed angle Aluminium 18000 27070 10 Round bottom 16 x 81 8 x 10 ml N.A. 121° C N.A. Speed Master 14/ 14R/ 18R

EFA15b Fixed angle Aluminium 15000 21800 50

15

15

Round bottom

Conical bottom

Round bottom

29 x 106

17 x 120

16.5 x 113

4 x 50 ml

4 x 15 ml

4 x 15 ml

N.A.

EFA1515C

EFA1515R

121° C N.A. Speed Master 14/ 14R/ 18R

EFA15C Fixed angle Aluminium 15000 23300 50

15

15

Round bottom

Conical bottom

Round bottom

29 x 104

17 x 120

16.5 x 113

6 x 50 ml

6 x 15 ml

6 x 15 ml

N.A.

EFA1515C

EFA1515R

121° C Aluminium Speed Master 14/ 14R/ 18R

ESW5 Swing out Aluminium 4800

4800

4800

4800

3000

3000

4800

4800

4800

4800

4800

4800

4170

4170

4170

4170

1630

1630

4170

4170

4170

4170

3,99

4170

250

5

7

10

10

15

15

15

15

50

50

50

Bottle

Vacutainer

Vacutainer

Vacutainer

Round bottom

Round bottom

Round bottom

Conical bottom

Conical bottom

Round bottom

Round bottom

Conical bottom

61.6 x 135

12-13 x 75-78

12-13 x 100

16 x 100

12 x 105

17 x 110

16.5 x 113

17 x 120

17 x 120

29 x 104

35 x 100

30 x 115

4 x 250 ml

28 x 5 ml

28 x 7 ml

28 x 10 ml

28 x 10 ml

28 x 15 ml

16 x 15 ml

12 x 15 ml

16 x 15 ml

4 x 50 ml

4 x 50 ml

4 x 50 ml

N.A.

ESW55B

ESW57B

ESW510B

ESW510G

ESW515G

ESW515P*

ESW515CA

ESW515CB*

ESW550P

ESW550G

ESW550C

121° C (buckets) Plastic lids with o-rings Only for Speed Master 18R

ESW5MP Swing out Aluminium 4800 3120 96 Well Microplates

96 Deep Well plates

1.5/2.0

0.2

PCR / Culture

PCR / Culture

Microtube

Single PCR tube or strip

128W x 86l x 14h

128W x 86l x 55h

10.8 x 40.5

2 x piastre

2 x piastre

48 x 1.5 / 2.0 ml

192 x 0.2 ml

N.A.

N.A.

ESW5MP15

ESW5MP02

121° C (buckets) N.A. Only for Speed Master 18R

ESW6 Swing out Aluminium 4000 2900 15

50

Conical bottom

Conical bottom

17 x 120

30 x 115

24 x 15 ml

8 x 50 ml

ESW615CA

ESW650C

121° C (buckets) N.A. Only for Speed Master 18R

List of Available Rotors

Technical Appendix 18

155

CODE PHOTO ROTOR TYPE MATERIAL MAX. SPEED (RPM) MAX. RCF (XG) VOLUME (ml) DESCRIPTION DIMENSIONS (ø x mm) QUANTITY ADAPTERS AUTOCLAVAbLE ROTOR LID CENTRIFUGE MODEL

EFA18b Fixed angle Aluminium 18000 24990 1.5 / 2.0

0:05

0:02

Microtube

PCR Microtube

PCR Microtube

10.5 x 41

8 x 32

6 x 24

18 x 1.5 / 2.0

18 x 0.5 ml

18 x 0.2 ml

N.A.

EFA1505

EFA1502

121° C Aluminium Only for Speed Master 18R

EFA15A Fixed angle Aluminium 15000 21500 1.5 / 2.0

0:05

0:02

Microtube

PCR Microtube

PCR Microtube

10.5 x 41

8 x 32

6 x 24

24 x 1.5 / 2.0 ml

24 x 0.5 ml

25 x 0.2 ml

N.A.

EFA1505

EFA1502

121° C Aluminium Speed Master 14/ 14R/ 18R

EFA12A Fixed angle Aluminium 12000 14100 0,2 PCR Microtube/strip 6 x 24 48 x 0,2 ml N.A. 121° C N.A. Speed Master 14/ 14R/ 18R

EFA18A Fixed angle Aluminium 18000 27070 10 Round bottom 16 x 81 8 x 10 ml N.A. 121° C N.A. Speed Master 14/ 14R/ 18R

EFA15b Fixed angle Aluminium 15000 21800 50

15

15

Round bottom

Conical bottom

Round bottom

29 x 106

17 x 120

16.5 x 113

4 x 50 ml

4 x 15 ml

4 x 15 ml

N.A.

EFA1515C

EFA1515R

121° C N.A. Speed Master 14/ 14R/ 18R

EFA15C Fixed angle Aluminium 15000 23300 50

15

15

Round bottom

Conical bottom

Round bottom

29 x 104

17 x 120

16.5 x 113

6 x 50 ml

6 x 15 ml

6 x 15 ml

N.A.

EFA1515C

EFA1515R

121° C Aluminium Speed Master 14/ 14R/ 18R

ESW5 Swing out Aluminium 4800

4800

4800

4800

3000

3000

4800

4800

4800

4800

4800

4800

4170

4170

4170

4170

1630

1630

4170

4170

4170

4170

3,99

4170

250

5

7

10

10

15

15

15

15

50

50

50

Bottle

Vacutainer

Vacutainer

Vacutainer

Round bottom

Round bottom

Round bottom

Conical bottom

Conical bottom

Round bottom

Round bottom

Conical bottom

61.6 x 135

12-13 x 75-78

12-13 x 100

16 x 100

12 x 105

17 x 110

16.5 x 113

17 x 120

17 x 120

29 x 104

35 x 100

30 x 115

4 x 250 ml

28 x 5 ml

28 x 7 ml

28 x 10 ml

28 x 10 ml

28 x 15 ml

16 x 15 ml

12 x 15 ml

16 x 15 ml

4 x 50 ml

4 x 50 ml

4 x 50 ml

N.A.

ESW55B

ESW57B

ESW510B

ESW510G

ESW515G

ESW515P*

ESW515CA

ESW515CB*

ESW550P

ESW550G

ESW550C

121° C (buckets) Plastic lids with o-rings Only for Speed Master 18R

ESW5MP Swing out Aluminium 4800 3120 96 Well Microplates

96 Deep Well plates

1.5/2.0

0.2

PCR / Culture

PCR / Culture

Microtube

Single PCR tube or strip

128W x 86l x 14h

128W x 86l x 55h

10.8 x 40.5

2 x piastre

2 x piastre

48 x 1.5 / 2.0 ml

192 x 0.2 ml

N.A.

N.A.

ESW5MP15

ESW5MP02

121° C (buckets) N.A. Only for Speed Master 18R

ESW6 Swing out Aluminium 4000 2900 15

50

Conical bottom

Conical bottom

17 x 120

30 x 115

24 x 15 ml

8 x 50 ml

ESW615CA

ESW650C

121° C (buckets) N.A. Only for Speed Master 18R