Upload
alexandro-bonifaz
View
216
Download
0
Embed Size (px)
Citation preview
700
International Journal of Dermatology 1999, 38, 700–711 © 1999 Blackwell Science Ltd
Cameo
Superficial cutaneous sporotrichosis in specific anergicpatient
Alexandro Bonifaz, MB, Amado Saul, MD, Griselda Montes-de-Oca, MD, andPatricia Mercadillo, MD
From the Dermatology Service, A 25-year-old man from Coscomatepec, Veracruz (400 km south-east of Mexico City), andDepartment of Mycology, and currently resident at this location, presented with dermatosis on the face, affecting theDermatopathology Service, General
frontal region, dorsum of the nose, and malar region, composed of two nodular orHospital of Mexico, Mexico D.F.,
verrucose plaques with an irregular diameter and form, with infiltrated borders,Mexicoulcerations, and bloody and honey crusts; the plaques developed over 4 years after
Correspondence trauma due to a horse kick (Fig. 1a.)Alexandro Bonifaz, MB Symptoms were moderate pruritus and some pain on examination. The patient hadZempoala 60–101
previously received topical treatment with steroids and procaine penicillin in an irregularNarvarte, CP 03020
form. At the beginning, the presumptive clinical diagnosis was verrucose tuberculosis;Mexico D.F.thus a tuberculin skin test (purified protein derivative, PPD) was applied with a responseMexico
of 3 3 2 cm of induration and erythema; later, a biopsy was taken to confirm theDrug names diagnosis. Based on these results, antituberculosis treatment with ethambutol, 1.2 g/day,amphotericin B: Fungizone
and isoniazid, 300 mg/day, plus copper sulfate baths, was instituted for 1 month.ethambutol 1 isoniazid: Myambuthol
Histopathology revealed a parakeratotic epidermis with moderate irregular acanthosis;fluconazole: Diflucanzones of spongiosis and exocytosis formed in some areas with crusts and cellularketoconazole: Nizoral
itraconazole: Sporanox detritus. In the superficial and medial dermis layers, a wide inflammatory infiltrate formedterbinafine: Lamisil from lymphocytes, epithelial histiocytes, some plasmocytes, and giant multinucleated
Langhans-type cells and some dilated and congested capillary vessels were observed.
Through Schiff’s and Grocott’s stains, long yeast-like structures of approximately 5–8 µm
were observed. The histopathologic diagnosis was a tuberculoid granuloma with mycotic
elements suggesting blastomycosis (Fig. 2).
Based on the histologic examination, the following mycologic tests were performed:
direct examination with potassium hydroxide (KOH) and with Gram stain revealed yeast-
like, oval and elongated cells of approximately 5–10 µm. Cultures of Sabouraud and
Micosel agar grew Sporothrix schenckii. Skin tests of sporotrichin M and Y (from mycelial
and yeast antigens) were applied several times at different concentrations, always with
negative results; therefore, the immunologic profile was performed (summary in Table 1).
Hematology, liver function tests, and urinalysis were within normal ranges. A chest X-
ray was normal. The CD4/CD8 lymphocyte ratio was 1.25 cell/mm3, i.e. within normal
values. Two evaluations of human immunodeficiency virus (HIV) antibodies performed in
two different periods were negative to Western blot (WB) and enzyme-linked
immunoabsorbent assay (ELISA) tests.
During the past 8 years, we have observed the patient; he has received several
treatments and has achieved considerable clinical improvement; nevertheless, each time
treatment is discontinued, new superficial and discrete lesions appear with positive
cultures to S. schenckii; sporotrichin tests were applied every year (8 times), and yielded
a positive result.
Figure 3 summarizes the treatment period as well as the drugs used. In summary, the
patient was managed as follows: he initially received oral potassium iodide, 3–6 g/day for
8 months; this treatment was repeated many times in several episodes of reactivation of
the disease or as supportive treatment; ketoconazole, 400 mg/day for 6 months;
amphotericin B for 2 months at a dose level of 0.50 mg/kg/day; itraconazole, 300–
400 mg/day for several treatment periods; fluconazole, 300 mg/day for 4 months;
Bonifaz et al. Superficial cutaneous sporotrichosis Cameo 701
terbinafine, 500 mg/day for 4 months. During the use of all these systemic antimycotic
agents, monitoring of hematology and renal and liver function tests were performed with
no alterations observed.
As a consequence of the initial trauma and its cicatrization, the patient presented
retraction of the left eyelid, for which he underwent a surgical process; bridal tissues were
released and a graft was placed later on with a satisfactory functional and cosmetic result.
Currently, the patient is alternately taking, with resting periods of up to 3 months,
potassium iodide at a dose of 0.5–1.0 g/day or itraconazole 100 mg/day. At the last visit
(March 1998), the patient showed excellent clinical improvement; however, cultures are
still positive.
Discussion
Sporothrix is a subcutaneous mycosis caused by a
dimorphic fungus Sporothrix schenckii. It is a poly-
morphic affection that may appear in different clinical
forms, depending on the patient’s immune condition. In
general, the most common clinical types are the lymphocut-
aneous and the fixed cutaneous; however, when the patient
presents a severe immunosuppression, the disease is mani-
fested as cutaneous-disseminated hematogenous, pulmon-
ary, etc.1–3
The purpose of this study is to report an exceptional
case of a superficial cutaneous sporotrichosis in a patient
with an immunologic base of specific anergy to S. schenckii
antigens; the therapeutic management of the patient is also
described. The patient has been controlled but not cured.
Sporotrichosis is one of the most frequent subcutaneous
mycoses in Mexico; the clinical variety commonly observed
in patients with good immunologic response is lympho-
cutaneous and, in a smaller proportion, fixed cutaneous;
however, in immunocompromised patients, the types
observed are cutaneous hematogenous, pulmonary, and
visceral forms.1,4 The present case is exceptional due to
the fact that it is a rare superficial cutaneous form and
cannot be classified under any of the above-mentioned
types. It has been previously reported by some authors.4,5
It is also important to mention that, at the beginning, it
was considered to be a verrucose tuberculosis because of
its form and its strong response to intradermal tuberculin,
as well as its frequency in Mexico.
The diagnosis of sporotrichosis is based on fungus culture
and the skin test response to mycelial sporotrichin; it is
worth noting that our patient has never responded in a
positive manner to both forms of the antigen and, according
to the immunologic studies performed, the amount of CD4
and CD8 lymphocytes was normal as their ratio was greater
than unity; in addition, he was positive to most antigens
in intradermal form and maintains a normal activity to
phagocytosis, which is considered an important barrier in
the course of sporotrichosis;6,7 however, negative responses
© 1999 Blackwell Science Ltd International Journal of Dermatology 1999, 38, 700–711
were always found when specific antigens to S. schenckii
(mycelial and yeast form) were used in vivo and in vitro.
Owing to these results, we consider that our patient
represents an extraordinary case of specific anergy: there
is no immune response, especially of the cellular type, to
S. schenckii antigens and this is probably the reason for
the unusual clinical form as well as the course of the
disease.4,8
From the histologic point of view, it is important to
Figure 1 (a) Initial appearance. (b) After 7 years of various
treatments
702 Cameo Superficial cutaneous sporotrichosis Bonifaz et al.
Figure 2 Skin biopsy: yeast cells of
Sporothrix schenckii (Grocott’s stain,
original magnification 3100)
Table 1 Immunologic features
Antigens Skin tests MIF (%)
PPD (tuberculin) 3 3 2 cm 77Candidin 2 3 2 cm 60Trichophytin 1 3 1 cm 36Streptokinase-streptodornase (varidase) Negative 20Sporotrichin Y (yeast) Negative 0Sporotrichin M (mycelial) Negative 0
MIF, macrophage inhibition factor.
Figure 3 Treatment and course of the disease
underline that basically a tuberculoid granuloma is present,
which clearly shows that there is a good immunologic
response; nevertheless, many yeast-like forms of S. schenckii
cells appear, which are only observed in immuno-
compromised patients, mainly in the hematogenous and
disseminated cases; this means that there is a bivalent
International Journal of Dermatology 1999, 38, 700–711 © 1999 Blackwell Science Ltd
behavior, with a strong defensive process (granuloma), but
which does not eliminate the fungus.9,10
The treatment of choice for sporotrichosis is potassium
iodide; it is important to mention that most cases respond
well to this treatment;1,4 also most of the systemic anti-
mycotic agents, such as griseofulvin, amphotericin B, keto-
conazole, itraconazole, fluconazole, and terbinafine, have
been used with good results.4,11–14 The mechanism of action
of potassium iodide is not well known; most investigators
consider that it possibly causes an immunologic stimulation
of polymorphonuclear leukocytes; this is why it is not very
useful in immunodepressed patients, such as those with
acquired immunodeficiency syndrome (AIDS), where the
use of a real antimycotic agent, such as those mentioned
above, is recommended.
Figure 3 shows the course of the disease, as well as the
antimycotic agents used throughout the 8 years of follow-
up; it can be seen that practically all the antimycotic agents
have been used in the appropriate dose and for the period
recommended by most authors; however, when the different
therapies have been discontinued, new small lesions appear,
from which S. schenckii has been isolated. We believe that
this is undoubtedly due to the specific anergy condition
which does not allow the eradication of the fungus.
Currently, the patient shows an important clinical
improvement (Fig. 1b), but he remains mycologically active,
being controlled with periods of low doses of potassium
iodide (0.5–1 g/day) and, alternatively, with itraconazole
(100 mg/day). We are aware that this treatment cannot be
maintained, as adverse events such as iodism or liver
damage may occur. It is important to note that the type of
disease presented by the patient is not life threatening nor
disseminating; one treatment which might help to eradicate
Peretz et al. Vegetative pyoderma gangrenosum Cameo 703
the fungus may be immunostimulation therapy with a
specific transference factor; however, donors with a positive
response to intradermal sporotrichin are needed for its
preparation.
The objective of this study was to present an exceptional
case of sporotrichosis from several points of view—clinical,
histologic, and therapeutic—allowing us to understand
more about the versatility of sporotrichosis.
References
1 Lavalle P, Mariat F. Sporotrichosis. Bull Inst Pasteur
1983; 81: 295–322.
2 Win RE. Sporotrichosis. Infect Clin Dis North Am 1988;
2: 899–911.
3 Werner AH, Werner BE. Sporotrichosis in man and
animal. Int J Dermatol 1994; 33: 692–700.
4 Saul A. Sporotrichosis. In: Jacobs PH, Nall L, eds.
Antifungal Drug Therapy. New York: Marcel Dekker,
1990: 53–59.
5 Campos P, Arenas R, Coronado H. Epidemic cutaneous
sporotrichosis. Int J Dermatol 1994; 33: 38–41.
6 Cunningham KM, Blumer GS, Rhoades ER.
Cameo
Vegetative pyoderma gangrenosum: an unusual presentationEyal Peretz, MD, Emanuela Cagnano, MD, Marcelo H. Grunwald, MD,Dafna Hallel-Halevy, MD, and Sima Halevy, MD
From the Department of Dermatology, A 44-year-old woman presented with a 6-month history of subcutaneous nodules involvingFaculty of Health Sciences, Soroka her face and shins. Her past history revealed myomatous uterus with menometrorrhagia,Medical Center, Ben-Gurion resulting in an iron deficiency anemia, treated by ferrum sulfate tablets. She also hadUniversity of the Negev, Beer-Sheva,
diabetes mellitus type II treated by glibenclamide and metformin tablets. There was noIsraelevidence of bromide or iodide ingestion. On initial examination, several firm, tender,
Correspondence erythematous subcutaneous nodules, 2–4 cm in diameter, were present on the faceEyal Peretz, MD (Fig. 1) and shins (Fig. 2), two with normal overlying skin. Within a few weeks, the noduleDepartment of Dermatology on the left shin enlarged and several superficial ulcers appeared, surrounded by purplishSoroka Medical Center
borders, secreting sinuses, and a vegetative exophytic surface (Fig. 3). PhysicalBen-Gurion University of the Negevexamination was normal. Incisional biopsies from the ulcer margins showedBeer-Sheva
Israel pseudoepitheliomatous hyperplasia, diffuse neutrophilic infiltration with microabscess
formation (Fig. .4), and the presence of a dense infiltrate composed of epithelioid cells,
giant cells, and a few lymphocytes forming non-necrotizing granulomas within the dermis
and subcutaneous lobules (Fig. 5). Leukocytoclastic vasculitis was also present in the
lower dermis. Routine blood tests and urine analysis were normal, except for a high
erythrocyte sedimentation rate (120/h) and anemia with a hemoglobin level of 10 g%. The
results of the following laboratory tests were found to be within normal limits: rheumatoid
factor; antinuclear antibody; C3; C4; serum immunoglobulins; serum protein
electrophoresis; stool for parasites and occult blood; X-ray of the chest, left shin, hands,
and facial bones; isotopic bone scanning; abdominal ultrasonography; chest and
© 1999 Blackwell Science Ltd International Journal of Dermatology 1999, 38, 700–711
Phagocytosis: an intracellular fate of Sporothrix
schenckii. J Infect Dis 1979; 140: 815–817.
7 Ramos-Zepeda R, Gonzalez-Mendoza A. Metabolic
activity of phagocytes in experimental sporotrichosis.
Mycopathologia 1986; 93: 109–112.
8 Everett MA. Atypical sporotrichosis. J Okla State Med
Assoc 1963; 56: 483–485.
9 Lurie HL. Histopathology of sporotrichosis. Arch Pathol
1963; 75: 121–137.
10 Novales J. Histopatologıa de las micosis profundas.
Dermat Rev Mex 1983; 27: 128–155.
11 Cullen S, Maucer AA, Warner N. Successful treatment of
disseminated cutaneous sporotrichosis with
ketoconazole. J Am Acad Dermatol 1992; 27: 463–464.
12 Sharkey-Mahis PK, Kauffman CA, Graybill JR, et al.
Treatment of sporotrichosis with itraconazole. Am J
Med 1993; 95: 279–285.
13 Castro LG, Belda W, Cuce LC, et al. Successful
treatment of sporotrichosis with oral fluconazole: a
report of three cases. Br J Dermatol 1993; 128:
352–356.
14 Hull PR, Vismer HF. Treatment of cutaneous
sporotrichosis with terbinafine. Br J Dermatol 1992;
126: 51–55.
704 Cameo Vegetative pyoderma gangrenosum Peretz et al.
abdominal computerized tomography. Repeated smears and tissue cultures for bacteria,
nmycobacteria, and fungi obtained from several ulcer sites were negative. Smears and
cultures for Leishmania and polymerase chain reaction (PCR) for Leishmania were
negative. Based on the clinical and histologic picture, our case was diagnosed as
vegetative pyoderma gangrenosum. The lesions did not respond to systemic antibacterial
agents including minocycline and cotrimoxazole. A marked improvement was observed
following treatment with prednisone at an initial dose of 40 mg/day. Relapse of the lesions
was observed after several attempts to reduce the steroid dose. Using dapsone as an
adjuvant therapy in increasing dose up to 250 mg/day induced significant improvement,
which allowed us to reduce the dose of prednisone to 10 mg/day. Dapsone was
discontinued after 3 weeks of treatment due to acute hepatitis induced by the drug. A few
days after cessation of dapsone, the cutaneous lesions recurred, and were only partially
controlled by a prednisone regimen of 10/20 mg on alternate days. Complete remission
was achieved after 6 months of therapy, leaving a small cribriform scar on the leg.
Discussion
The classic ulcerative form of pyoderma gangrenosum (PG)
is known to have three variants, including pustular, bullous,
and vegetative forms. Vegetative pyoderma gangrenosum
(VPG), also known as pyoderma vegetans and superficial
granulomatous pyoderma, is a recently recognized variant
of PG, having unique clinical, histologic, and therapeutic
characteristics.1,2 Unlike the ulcerative form of PG
(Table 1), the initial lesion of VPG is a nodule or plaque,
solitary or multiple,1 which evolves to form superficial
ulcers with a clean base and purple borders.3 The lesions
may be exophytic, vegetative, slowly progressive, and
relatively nonpainful,4 most commonly located on the
back, but can involve any part of the trunk and limbs.1,3
Histologically, a three-layer inflammatory reaction is pre-
sent.5 The first layer consists of superficial abscess formation
in the upper dermis. The second layer demonstrates granul-
oma formation with histiocytes and multinucleated giant
cells. The surrounding third layer is composed of a mixed
Figure 1 Erythematous nodule on the chin
International Journal of Dermatology 1999, 38, 700–711 © 1999 Blackwell Science Ltd
inflammatory infiltrate with lymphocytes, numerous plasma
cells, neutrophils, and eosinophils. Pseudoepitheliomatous
hyperplasia of the epidermis may be present.5 Treatment
with topical steroids or with antimicrobial agents, such as
tetracycline, minocycline, dapsone, and sulfapyridine, is
usually sufficient to control the disease activity.3 The unique
Figure 2 Erythematous vegetative lesion on the leg
Peretz et al. Vegetative pyoderma gangrenosum Cameo 705
Table 1 Comparative features between VPG and PG
VPG PG
Location Most commonly the trunk and upper limbs Most commonly the lower limbs
Primary lesion Nodule or plaque Pastule
Number (lesions) Solitary or multiple Usually solitary
Ulcer (characteristics) Superficial, purplish determined border, clean base, may Deep, purplish undetermined border, purulent base,be exophytic, vegetative, slowly progressive, usually not rapidly enlarges, painfulpainful
Histology Three layers: dermal neutrophilic abscesses, Extensive dermal neutrophilic abscesses, sometimesgranulomas, mixed inflammatory infiltrate vasculitis
Treatment Response to simple modes of therapy (topical steroids, Aggressive therapy (systemic steroids,antibacterial agents) immunosuppressive agents)
Associated disease Usually no associated disease Frequently present (IBD, monoclonal gammopathy)
IBD, inflammatory bowel disease.
Figure 3 Superficial ulcers with vegetative surface surrounded
by purplish border
findings in this case in comparison with previous reports
of VPG include the unusual location on the face, the
associated tenderness of the lesions, and the presence of
© 1999 Blackwell Science Ltd International Journal of Dermatology 1999, 38, 700–711
Figure 4 Microabscess formation in the dermis (hematoxylin
and eosin stain; 3100)
nodules with normal overlying skin. Histologically, in
addition to as the characteristic findings of VPG, the
biopsies demonstrated some other unique findings, such
as vasculitis and granulomatous panniculitis. The partial
resistance of the lesions to systemic steroids is also an
unexpected behavior of VPG. The differential diagnosis of
our case should include the following diseases: periarteritis
nodosa, deep leukocytoclastic vasculitis, and local granulo-
matous vasculitis. Periarteritis nodosa and deep leuko-
cytoclastic vasculitis, unlike VPG, involve mainly the
subcutaneous septae, while in our case the subcutaneous
lobules were primarily involved. Furthermore, granulomas
are not present in these entities. Although VPG is considered
to be a superficial variant of ulcerative PG, it may also be
classified as a variant of granulomatous vasculitis.
706 Cameo Henoch–Schonlein purpura Goldberg, Shoji, and Sapadin
Figure 5 Granuloma formation in the deep dermis and
subcutis (hematoxylin and eosin stain; 3400)
Cameo
Henoch–Scho nlein purpura induced by clarithromycin
Elizabeth I. Goldberg, MD, Toru Shoji, MD, PhD, and Allen N. Sapadin, MD
From the Department of Dermatology, An 84-year-old Indian woman with no significant past medical history and no known drugMount Sinai School of Medicine, allergies had been prescribed clarithromycin (250 mg twice daily) for pneumonia. TheNew York, New York patient was receiving no other medications. Ten days after starting treatment, the patient
developed a mild fever, eruption, and swelling of the ankles. Several days later, theCorrespondencepatient developed a spreading, nonpainful, nonpruritic eruption, joint pain, gastrointestinalAllen N. Sapadin, MD
One Gustave L. Levy Place bleeding, and general malaise. Skin examination revealed numerous palpable purpuricBox 1048 macules and papules and petechiae on the lower extremities, mostly below the knees,New York, NY 10029 and on the right hand. There were large blistering lesions around both ankles, some of
which had ulcerated and had a necrotic center (Fig. 1). Blood streaked stool was notedDrug namesduring rectal examination. Laboratory tests showed a normal white blood cell count,clarithromycin: Biaxin
omeprazole: Prilosec hematocrit, and hemoglobin. Serum urea nitrogen was 22 mg/dL (8–18 mg/dL) and
creatinine was normal. Urinalysis revealed proteinuria of 0.9 g/24 h (,0.15 g/24 h) and a
microscopic hematuria. Antistreptolysin O, antinuclear antibodies, cryoglobulins, and
hepatitis serologies were all negative. Histology of the skin showed leukocytoclastic
International Journal of Dermatology 1999, 38, 700–711 © 1999 Blackwell Science Ltd
In summary, we have presented a case of VPG, a variant
of PG with unusual clinical and histologic characteristics.
Our case shows that even a variant of PG can have a broad
spectrum of clinical and histologic features.
References
1 Wilson-Jones E, Winkelmann RK. Superficial
granulomatous pyoderma: a localized vegetative form of
pyoderma gangrenosum. J Am Acad Dermatol 1988; 18:
511–521.
2 Quimby SR, Gibson LE, Winkelmann RK. Superficial
granulomatous pyoderma: clinicopathologic spectrum.
Mayo Clin Proc 1989; 64: 37–43.
3 Lichter MD, Welykyi SE, Gradini R, et al. Superficial
granulomatous pyoderma. Int J Dermatol 1991; 30:
418–421.
4 Powell FC, Su WPD, Perry HO. Pyoderma gangrenosum.
Classification and management. J Am Acad Dermatol
1996; 34: 395–409.
5 Schorter AL. Superficial granulomatous pyoderma,
Editorial. Mayo Clin Proc 1989; 64: 123–124.
Goldberg, Shoji, and Sapadin Henoch–Schonlein purpura Cameo 707
vasculitis of superficial vessels with extravasation of red blood cells (Fig. 2). Direct
immunofluorescence revealed immunoglobulin A (IgA) in superficial dermal vessels.
Treatment with prednisone (1 mg/kg/day) was started and the arthralgias, hematochezia,
and eruption resolved over the next 3 days. Renal function remained impaired, however,
and the patient was discharged with a proteinuria of 0.56 g/24 h (,0.15 g/day) on
prednisone and omeprazole.
Discussion
Clarithromycin is a macrolide antibiotic with improved
pharmacokinetics and lower frequency of adverse effects
relative to the parent compound erythromycin. It is used
for the treatment of mild to moderate upper and lower
respiratory tract infections, skin and soft tissue infections,
and infection due to Mycobacterium avium complex and
other nontuberculous mycobacteria.1 Adverse effects occur
in approximately 15–20% of patients and are almost
always mild. These primarily involve the gastrointestinal
tract.2 Cutaneous effects are rare. Two reported cases of
leukocytoclastic vasculitis due to clarithromycin have been
described.3,4
Henoch–Schonlein purpura (HSP) is an immune complex
disorder with leukocytoclastic vasculitis of the small vessels
of affected organs. This syndrome typically involves the
skin, joints, gastrointestinal tract, and kidneys. Although
more prevalent in the pediatric population, it may occur
in adults, in whom it is more likely to present with
ulcerative or necrotic cutaneous lesions.5 Most patients
will recover with an uncomplicated course lasting about 4
weeks, but 1–2% of patients will have persistent renal
function impairment.6 Corticosteroids have been reported
to relieve symptoms but not to alter the course.
Although the etiology remains unknown, several anti-
gens, including medications and infections, have been
Figure 1 Extensive purpura on the distal legs. Overlying the
medial malleolus of the left ankle is a large crusted erosion.
An ischemic but intact blister roof is apparent at the
periphery
© 1999 Blackwell Science Ltd International Journal of Dermatology 1999, 38, 700–711
implicated. Drug-induced HSP is a common association in
adults.7 Cases, although rare, have been reported related
to the macrolide antibiotics, including erythromycin,8 spira-
mycin,9 and midecamycin.10
The close temporal relation between drug exposure and
the onset of symptoms suggests that clarithromycin was
the most likely etiologic agent for HSP in our patient. To
our knowledge, this is the first case of HSP associated with
clarithromycin. Although rare, clinicians must be aware of
the possibility of HSP as a severe adverse effect when
prescribing such a widely used drug.
Figure 2 Medium power picture showing fibrinoid necrosis
of superficial vessels, neutrophilic infiltrate, and
karyorrhectic debris (hematoxylin and eosin, 3100)
708 Cameo Lichen sclerosus et atrophicus in GVHD Cordoba et al.
References
1 Wood MJ. The tolerance and toxicity of clarithromycin.
J Hosp Infect 1991; 19 (Suppl A): 39–46.
2 Pichotta P, Gupta S, Prokocimer P, Pernet A. The overall
safety of oral clarithromycin in comparative clinical
studies (abstract 1332). In: Abstracts of the
30th Interscience Conference on Antimicrobial Agents
and Chemotherapy, Atlanta, GA, 1990.
3 Gavura SR, Nusinowitz S. Leukocytoclastic vasculitis
associated with clarithromycin. Ann Pharmacother
1998; 32: 543–545.
4 de Vega T, Blanco S, Lopez C, et al. Clarithromycin-
induced leukocytoclastic vasculitis. Eur J Clin Microbiol
Infect Dis 1993; 12: 563.
5 Michel BA, Hunder GG, Bloch DA, Calabrese LH.
Hypersensitivity vasculitis and Henoch–Schonlein
Cameo
Lichen sclerosus et atrophicus in sclerodermatous chronicgraft-versus-host disease
Susana Cordoba, MD, Erich Vargas, MD, Javier Fraga, MD, PhD,Maximiliano Aragues, MD, Jesus Fernandez-Herrera, MD, PhD, andAmaro Garcıa-Dıez, MD, PhD
From the Departments of In May 1994, a 40-year-old woman with chronic myeloid leukemia received an allogeneicDermatology bone marrow transplant (BMT) from her human leukocyte antigen (HLA) identical sister,and Pathology, Hospital Universitario after a conditioning regimen with cyclophosphamide and busulfan. Graft-versus-hostde la Princesa, Madrid, Spain
disease (GVHD) prophylaxis consisted of cyclosporine (CsA) and methotrexate. Facial
and palmoplantar erythema and moderate cholestasis developed on day 14 after theCorrespondenceSusana Cordoba, MD BMT. A diagnosis of acute GVHD was made and she was successfully treated with lowServicio de Dermatologıa doses of corticosteroids. On day 150 after the BMT, despite the prophylactic treatment ofHospital Universitario de la Princesa GVHD with CsA (150 mg/12 h), she developed several burning white plaque-like striaeC/Diego de Leon 62
over the buccal mucosa and numerous itching violaceous lichenoid papules on the28006 Madridfingertips. Biopsy specimens obtained from both the skin of the fingertips and the oralSpainmucosa (Fig. 1) revealed patchy to diffuse subepithelial lymphocytic inflammation and
necrosis of individual squamous cells, consistent with a diagnosis of chronic lichenoid
GVHD. Despite therapy with CsA, topical and systemic corticosteroids (prednisone 60 mg/
24 h), the oral lichenoid lesions persisted. On day 750 after the BMT, 2 months after
withdrawal of immunosuppressive therapy, she developed several erythematous,
pruriginous, and slight indurated lesions over the neck. These lesions coalesced into
plaques, adopting a white atrophic-like appearance with follicular plugs similar to lichen
sclerosus et atrophicus (Fig. 2). Histopathologic examination showed hyperkeratosis with
follicular plugging, atrophy of the stratum Malpighii with hydropic degeneration of the
basal cells, homogenization of the collagen, incontinence of the pigment, and a discrete
lymphoplasmocytic inflammatory infiltrate in the upper dermis (Fig. 3). Systemic
corticosteroid therapy was re-introduced. On day 850 after the BMT, physical examination
revealed patchy hyperpigmentation affecting the back and limbs, and diffuse thickening
and hardening of the skin of the legs, forearms, and dorsa of the hands, resulting in
International Journal of Dermatology 1999, 38, 700–711 © 1999 Blackwell Science Ltd
purpura: a comparison between the two disorders. J
Rheumatol 1992; 19: 721–728.
6 Heng MCY. Henoch–Schonlein purpura. Br J Dermatol
1974; 109: 664.
7 Cream JJ, Gumpel JM, Peachy RDG. Schonlein–Henoch
purpura in the adult. Q Med 1970; 39: 461–484.
8 Handa SP. The Schonlein–Henoch purpura:
glomerulonephritis following erythromycin. South Med J
1972; 65: 917–920.
9 Valero PI, Calvo CJ, Hortelano ME, et al. Schonlein–
Henoch purpura associated with spiramycin and with
important digestive manifestations. Rev Esp Enferm Dig
1994; 85: 47–49.
10 Sanchez de Dios M, Sanchez Sanchez A, Garcia O.
Anaphylactoid Schonlein–Henoch purpura caused by
midecamycin. Rev Clin Esp 1992; 191: 230–231.
Cordoba et al. Lichen sclerosus et atrophicus in GVHD Cameo 709
a slight limitation of movements. Various smooth, shiny, and indurated plaques,
characteristic of morphea, were present on the flexural areas of the arms (at vein
puncture sites). Lichenoid lesions over the oral mucosa and the atrophic plaques on the
neck were still present. No dysphagia was observed. Liver function tests revealed a
pattern of cholestasis. Antinuclear antibodies were detected (1 : 80); no antibodies against
centromere antigens and DNA topoisomerase I (SCL70) were found. Reduced lacrimal
secretion (Schirmer test ,10 mm in 5 min) and a reduced forced expiratory volume in 1 s
(FEV1) were documented. A biopsy specimen from a sclerodermatous area showed a
thickened reticular and papillary dermis with closely packed collagen bundles and
abundant melanophages in the dermis (Fig. 4). Azathioprine was added to the
corticosteroid therapy in an attempt to arrest the progression of the disease. Six months
after starting treatment, cutaneous lesions still persist, but the patient has not developed
any other systemic symptoms.
Discussion
Chronic graft-versus-host disease (GVHD) is the major late
complication of allogeneic bone marrow transplantation.
Between 25% and 45% of the long-term allogeneic sur-
vivors develop the polymorphous syndrome of chronic
GVHD.1–3 Clinically, pathologically, and immunologically
it resembles an autoimmune disease that shares features
with systemic sclerosis, lichen planus, Sjogren syndrome,
and primary biliary cirrhosis.2,4–6 Chronic GVHD is a
multi-organ disease in which skin involvement is almost a
constant feature. It is initially characterized by lichen
planus-like lesions, whereas sclerodermatous changes occur
later and far less frequently.1,3,6–8 We report a case of
chronic GVHD showing the coexistence of lichen sclerosus
et atrophicus and sclerodermatous lesions, which to the
best of our knowledge has not been reported before.
Cutaneous changes are virtually always present in chronic
Figure 1 Diffuse subepithelial
lymphocytic inflammation and
satellite cell necrosis of squamous cells
in oral mucosa (hematoxylin and
eosin, 325)
© 1999 Blackwell Science Ltd International Journal of Dermatology 1999, 38, 700–711
GVHD and two distinct patterns, lichenoid and scleroderm-
atous, may be identified. We must emphasize the simultan-
eous coexistence of lichen sclerosus et atrophicus changes
and indurated morphea-like plaques in our patient. The
concurrent relationship of lichen sclerosus to localized
scleroderma is well recognized,9–11 and it has been noted
that the coexistence of the two entities is not uncommon.11
Our working group has reported several cases in which
changes of both morphea and lichen sclerosus et atrophicus
were present in the same lesion.10 An inflammatory cellular
infiltrate that extends from the deep dermis to the subcuta-
neous fat is the main finding in morphea. In lichen sclerosus,
however, it involves the upper reticular and papillary
dermis with hyalinization of the collagen. Some authors
have proposed that lichen sclerosus et atrophicus is a
superficial form of morphea.9 These pathologic findings
support the concept that the localized sclerodermatous
710 Cameo Lichen sclerosus et atrophicus in GVHD Cordoba et al.
conditions are a spectrum of diseases. Histopathologic
changes are variable and correlate with the clinical appear-
ance. Chronic GVHD is a pseudosclerodermatous condition
that may mimic the skin and extracutaneous manifestations
found in localized and systemic scleroderma. Some authors,
however, have found several differences in the sclerotic
phase of chronic GVHD, when compared with the ultra-
structural findings of idiopathic scleroderma; in the former,
inflammation and fibrosis are first observed in the papillary
dermis and the staining for procollagen I and III and
collagen III is heavier in the papillary dermis.12 Antismooth
muscle, antimitochondrial and antinuclear antibodies can
be detected in chronic GVHD, but they are usually found
in extensive disease with multiple organ involvement.5,13
As chronic GVHD involves the papillary dermis, it should
show a superficial morphea variant (lichen sclerosus et
atrophicus). For unknown reasons, however, it is uncom-
Figure 2 Atrophic and confluent plaques with follicular plugs
on the neck
Figure 3 Follicular plugging, atrophy
of the stratum Malpighii, hydropic
degeneration of basal cells with a split
visible at the dermoepidermal
junction, and homogenization of the
collagen in the upper dermis
(hematoxylin and eosin, 325)
International Journal of Dermatology 1999, 38, 700–711 © 1999 Blackwell Science Ltd
mon. We have not found any previously reported case of
lichen sclerosus et atrophicus-like lesions in chronic GVHD.
In 1990, it was reported that the mucocutaneous manifesta-
tions of chronic GVHD may clinically resemble a wide
variety of skin diseases including lichen sclerosus et atro-
phicus.14 In 1992, two patients with chronic sclerodermat-
ous GVHD, in whom extensive sclerotic lesions were
associated with multiple atrophic pearly white plaques and
ulceration, were described.1 The lesions resembled lichen
sclerosus et atrophicus-like lesions in clinical appearance.
The clinical outcome was fatal in both cases and this
atrophic and sclerotic pattern was considered as a marker
of severe chronic GVHD. Limited chronic GVHD has a
favorable prognosis, whereas patients with extensive dis-
ease require early treatment before clinical deterioration.
References
1 Chosidow O, Bagot M, Vernant JP. Sclerodermatous
chronic graft-versus-host disease. Analysis of seven cases.
J Am Acad Dermatol 1992; 26: 49–55.
2 Sullivan KM, Shulman HM, Storb R. Chronic graft-
versus-host disease in 52 patients: adverse natural course
and successful treatment with combination
immunosuppression. Blood 1981; 57: 267–276.
3 Aractingi S, Janin A, Devergie A, et al. Histochemical
and ultrastructural study of diffuse melanoderma after
bone marrow transplantation. Br J Dermatol 1996; 134:
325–331.
4 Atkinson K. Chronic graft-versus-host disease. Rev Bone
Marrow Transplant 1990; 5: 69–82.
5 Siegert W, Stemerowicz R, Hopf U. Antimitochondrial
antibodies in patients with chronic graft-versus-host
disease. Bone Marrow Transplant 1992; 10: 221–227.
Cordoba et al. Lichen sclerosus et atrophicus in GVHD Cameo 711
Figure 4 Thick collagen bundles in
reticular and papillary dermis
(hematoxylin and eosin, 310)
6 Spielvogel MR, Goltz RW, Kersey JH. Scleroderma-like
changes in chronic graft-versus-host disease. Arch
Dermatol 1977; 113: 1424–1428.
7 Aragues M, Fraga J, Fernandez-Herrera JM, et al.
Enfermedad injerto contra huesped cutanea. Estudio
clınico e histopatologico. Actas Dermo-Sif 1987; 78:
7–11.
8 Graham-Brown RAC, Sarkany I. Scleroderma-like
changes due to chronic graft-versus-host disease. Clin
Exp Dermatol 1983; 8: 531–538.
9 Conelly MG, Winkelman RK. Coexistence of lichen
sclerosus et atrophicus, morphea and lichen planus.
J Am Acad Dermatol 1985; 12: 844–851.
10 Buezo GF, Penas PF, Dorado JM, et al. Liquen
escleroatrofico y morfea. Actas Dermo-Sif 1994; 85:
201–205.
From the collection of Lawrence Charles Parish, MD, Philadelphia,
Pennsylvania.
© 1999 Blackwell Science Ltd International Journal of Dermatology 1999, 38, 700–711
11 Jablonska S. Differential diagnosis of scleroderma-like
disorders. In: Clements PJ, Furst DE, eds. Systemic
Sclerosis. Baltimore: Williams & Wilkins, 1997: 99–119.
12 Tabata H, Yamakage A, Yamazaki S. Electron-
microscopic study of sclerodermatous chronic graft-
versus-host disease. Int J Dermatol 1996; 35: 862–866.
13 Shulman HM, Sullivan KM, Weiden PL. Chronic graft-
versus-host syndrome in man. A long-term
clinicopathologic study of 20 Seattle patients. Am J Med
1980; 69: 204–217.
14 Volc-Platzer B, Stingl G. Cutaneous graft-vs-host disease.
In: Burakoff SJ, et al. eds. Graft-Vs-Host Disease
Immunology, Pathophysiology and Treatment. New
York: Marcel Dekker, 1990: 245–253.