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©2019 Sony Biotechnology Inc. All rights reserved. Sony and the Sony logo are trademarks of Sony Corporation. The ID7000 is a trademark of Sony Biotechnology Inc. All other trademarks are property of their respective owners. For non-clinical research use only. Not for use in diagnostic or therapeutic procedures, or for any other clinical purpose. The ID7000 Spectral Analyzer is classified as a Class 1 laser product.
9.11.062319.1
4-14-1, Asahi-cho, Atsugi-shi, Kanagawa, 243-0014 Japan Tel: +81 120-677-010 Fax: +81 120-388-060 [email protected]://www.sony.co.jp/LS
North America/International Japan Europe
1730 North First StreetSan Jose, CA 95112 USATel: +1 800-275-5963FAX: +1 [email protected]://www.sonybiotechnology.com
The Heights, Brooklands. Weybridge, Surrey, KT13 0XW, UK Tel: +44 (0) 1932 817448 [email protected] https://www.sonybiotechnology.com
Optics Excitation optics 320 nm, 355 nm, 405 nm, 488 nm, 561 nm, 637 nm, 808 nm
Configurations 3, 4, 5, 6, and 7 lasers
Autoloader (standard)
Sample formats supported
Standard and deep 96- and 384-well plates, and up to twenty-four (12 x 75-mm) 5-mL tubes in the tube loader
Sample cooling Active cooling maintains temperature of stage to reduce variability and prevent sample degradation.
Sample agitation Active agitation keeps cells in suspension during acquisition.
Software Data formats Flow Cytometry Standard (FCS) 3.1, CSV, SRAW, PDF
Sony Biotechnology Inc.
ID7000™Spectral Cell AnalyzerA high-parameter spectral cytometer, the ID7000 delivers comprehensive information about heterogeneous cell populations, with high sensitivity to detect dim and rare populations.
Expanding the boundaries of cell analysis, the ID7000 can be configured with up to 7 lasers and 188 detectors, the most of any flow cytometer available today. The system enables researchers to perform experiments using 44 colors, limited only by the fluorochromes available. The choice of lasers include blue (488 nm), red (637 nm), violet (405 nm), yellow-green (561 nm), ultraviolet (355 nm), deep ultra-violet (320 nm), and infrared (808 nm).
Streamline High-Parameter Multicolor Workflows The ID7000 provides capabilities to simplify high-param-eter experiments and streamline multicolor workflows. Novel features include the Spectral Reference Library and the Autofluorescence Finder. The Spectral Reference Library stores information from single-positive controls for each reagent for future use, so researchers need to run controls only once, saving time. With the Auto-fluorescence Finder, researchers can identify and subtract contributions from multiple autofluorescent populations for higher fidelity data and more accurate visualization.
The ID7000 uses spectral technology to collect continuous light from 360 nm to 920 nm, allowing researchers to obtain more data than from a traditional cytometer. With this technology, data can be measured more accurately, and both spectrally adjacent fluorochromes and fluorescent proteins can be used in a panel.
Software and automation simplify operations to save time and improve consistency
Supports a high number of lasers and configurability to empower deep scientific insights
Simplify Operations for True Ease of Use
Software wizards and tools simplify instrument controls from startup to shutdown, automate quality control, and optimize experiment creation. For example, the system’s AutoSampler includes a unique, active agitation capability to keep cells in suspension during acquisi-tion. Also, an active cooling function further reduces variability and prevents sample degradation over time. Software and automation manage error conditions such as bubbles, clogs, and low sample volume, and enable walkaway operation.
Harness Complex Data The ID7000 system builds on Sony’s experience with spectral analysis and simplifies many operations, even for complex experiments. The system collects the entire spectrum of light from 360 nm to 920 nm, which allows researchers to separate fluorochromes into pure signals to more accurately measure data. In comparison, conven-tional flow cytometry uses color compensation to deter-mine each fluorescence intensity by subtracting overlap-ping fluorescence, resulting in gaps in the collected light. Thus, some of the data is lost.
Optics design and spectral technology make it possible for researchers to use both spectrally adjacent fluoro-chromes and fluorescent proteins in a panel—a capability that is not practical using a traditional cytometer.
A powerful capability of spectral technology is unmixing. Spectral unmixing objectively manages overlap between fluorescence spectra to improve data accuracy. A novel auto-unmixing capability, and new algorithms, speed processing for ease of operation. Spectral unmixing elimi-nates the subjectivity and complexity associated with manual compensation methods.
The AutoSampler supports a wide variety of standard and deep 96- and 384-well plates, and up to twenty-four (12 x 75-mm) 5-mL tubes in the tube loader.
Spectral Reference LibrarySpectral unmixing separates each spectral fingerprint for complete and optimal visualization of fluorochromes
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In the above graph, the blue line shows the event rate when the active agitation is applied for 2 seconds at 180-second intervals, keeping the sample in suspension. The brown line shows agitation for 2 seconds only, resulting in cells settling over time.
