NANO-ENGINEERED ANTIBACTERIAL COATING FOR YOUR HOME

Works against E.Coli, Staphylococcus Aureus, Bacillus Subtilis, S.Typhi, Klebsiella Pneumoniae

BIOZINY IS ONE WAY TO DEAL WITH ANTIMICROBIAL

RESISTANCE (AMR)

*The Packaging of the actual product may vary from the one shown above.

What is BioZiny? BioZiny is water based nano-engineered antibacterial coating. BioZiny is nano zinc oxide (ZnO) based, easy to apply, transparent, aqueous Coating for high touch surfaces at home, hospitals, medical centers, schools, busses, trains, airplanes, busses and other public places like shopping malls, Supermarkets, children’s play areas etc.

Special Features: Superior Antibacterial properties - Kills 99.99% Bacteria within 24 Hours of application Shields the surface from bacteria for six months* The coating uses nanoparticles of Zinc Oxide as active ingredient. Zinc Oxide is generally recognized as safe (GRAS) material by the U.S. Food and Drug Administration (21CFR182.8991) (FDA, 2011). The coating is water based, hence no VOC and no associated health hazards. Can be used on Steel, Aluminum, all kinds of plastics and wood surfaces

Superior Transparency

*Only when the surface is not treated with strong acid or alkali; when the surface is not scrubbed with hard scrubbers like steel wool or ceramic particle coated scrubber. The coated surface, if at all washed, should be washed with detergent and normal water having room temperature.

As per standard tests conducted in a laboratory, BioZiny has the abil ity to kill 99.99% of E.Coli and 99.99 % of Staphylococcus Aureus within 24 hours of application

Results as Per JIS Z 2801:2010

*Test Results are for a particular sample under laboratory conditions.

0

20

40

60

80

100

0 4 8 12 16

% Reduction in Bacterial Count

Staphylococcus Aureus

%

Bacterial Count of Untreated Sample at T=0

Bacterial Count of Untreated Sample at T= 24Hrs (U)

Log (U)

Bacterial Count of Treated Sample at T=24 Hrs, T

Log(T)

‘R’ = Log(U)-Log(T)

Why should you use BioZiny? BioZiny shields your home from harmful bacteria. BioZiny, after proper application and drying, has the ability to kill 99.99% bacteria that thrives on the exposed surfaces, thereby protecting your near and dear ones, and yourself, from harmful infections caused by bacteria.

As per standard tests conducted in a laboratory, BioZiny has the abili ty to kill 99.99% of E.Coli and 99.99 % of Staphylococcus Aureus within 24 hours of applicat ion

Results as Per JIS Z 2801:2010

*Test Results are for a particular sample under laboratory conditions. We provide no guarantee on the replication of the same result in other conditions.

16 20 24

% Reduction in Bacterial Count

Aureus Hrs0

20

40

60

80

100

0 4 8 12

% Reduction in Bacterial Count

E.Coli

%

Staphylococcus Aureus

Bacterial Count of Untreated Sample at T=0 1.86030E+5

Bacterial Count of Untreated Sample at T= 24Hrs (U) 3.383100E+6

6.58

Bacterial Count of Treated Sample at T=24 Hrs, T 1.760E+3

3.24

3.34

Why should you use BioZiny? BioZiny shields your home from harmful bacteria.

BioZiny, after proper application and drying, has the ability to kill 99.99% bacteria that thrives on the exposed surfaces, thereby protecting your near and dear ones, and yourself, from harmful

Mapping of Bacteri

As per standard tests conducted in a laboratory, BioZiny has the ability to kil l 99.99% of E.Coli and 99.99 % of Staphylococcus Aureus within 24 hours of application*.

12 16 20 24

% Reduction in Bacterial Count

Hrs

Staphylococcus Aureus E.Coli

2.93750E+5

1.527000E+6

6.18

2.938E+3

3.47

2.71

Mapping of Bacteria in the hand of a 5 year old after playing outdoors

BioZiny works against Antimicrobial Resistance (AMR).

Antibiotics have a downside: The more often these drugs are used, the more quickly bugs outsmart them.

Other than antibiotics, BioZiny is another way to deal with Bugs

Antimicrobial Resistance is the ability of a microorganism (like Bacteria, Viruses, and some parasites) to stop an antimicrobial (such as antibiotic, antivirals and antimalarials) from working against it. As a result standard treatments become ineffective, infections persist and may spread to others. (Source: WHO)

How to Apply BioZiny*?

Understand that BioZiny is not a polishing or cleaning liquid agent. BioZiny need not be rubbed on the surface.

Clean the surface thoroughly with plane water or detergent before applying

BioZiny

Make sure there is no dust, dirt, oil or grease on the surface

Wipe the surface with a dry cloth before applying and make sure the surface is dry.

Apply the coating on the applicator wipe that is supplied with the coating

with a generous amount of liquid

Before applying the coating on the wipe, the wipe may be cut into a suitable size.

Apply the coating with the help of this applicator wipe on the surface with

soft strokes.

Do not buff or try rubbing once a visible clear wet coat is formed.

Apply the coating uniformly on the surface.

Make sure you spread the extra liquid uniformly on the surface softly without rubbing/buffing.

Do not buff, the surface remains wet & sticky after application and should be left to dry.

Allow it to dry on its own for 24 hours for the coating to deliver its full value.

*For best results the coating has to be applied as per given instructions. A Wrong application procedure may not lead to desired results. Application is the most important part of the process.

Where is BioZiny applied? BioZiny is applied on high touch surfaces in your home, in hospitals, medical centres, schools, trains, airplanes, busses and other public places like shopping malls, children’s play areas etc. BioZiny can be applied on almost all substrates like Stainless Steel, Aluminium, metal, all kinds of plastics and wood.

Probable places at home where BioZiny can be applied are:

- All Faucets - Door Handles - Tiles - Toilet Seat - Wash Basin - Commode - Kitchen Sink - Kitchen Sink Faucet - High Touch Areas in Wooden

Furniture - Refrigerator Handles - Wardrobe Handles - Kitchen Cabinet Handles - Kitchen Top - Gas Stove Knobs - Switch Boards - Tooth Brush Holder - Leather Items - All other steel surfaces like

steel hand rails at home and staircases Many Others

Nano Zinc Oxide acts on bacteria on one or more of the following ways

Very High Specific Surface Area:

Particles are less than 100nm in diameter thus having more pronounced antibacterial activities than large particles since the small size and high specific surface area allow better interaction with bacteria.

What is the active ingredient in BioZiny? The active ingredient in BioZiny is surface modified Nano Zinc Oxide. Nano Zinc Oxide has demonstrated antibacterial properties against both Gram Positive and Gram Negative Bacteria. The antibacterial and antifungal properties of nano-zinc oxide have been thoroughly studied and the results are ubiquitous in scientific literature. The list of publications listed below extensively talks about the Antibacterial and Antimicrobial Properties of Nano ZnO.

Release of Antimicrobial Ions: Zinc Ions (Zn2+) exhibit antimicrobial activity. In aqueous suspension or when in contact with water, Zinc Oxide particles release Zinc Ions (Zn2+) that contributes to elimination of bacteria.

Direct Interaction of Nano ZnO particles with Bacteria:

The interaction between ZnO nanoparticles and bacterial cells is caused by electrostatic forces. The global charge of bacterial cells at biological pH values is negative, due to the excess of carboxylic groups, which are dissociated and provide a negative charge to the cell surface. On the other hand ZnO nanoparticles have a positive charge. As a result, opposite charges between the bacteria and Nanoparticles generate electrostatic forces, leading to a strong bind between the nanoparticles and the bacteria surface leading to disruption of the cell walls causing internalization of the nanoparticles in the bacterial cells. Nano ZnO causes great damage to the bacterial cells like disorganization of the cell walls, alteration of morphology and internal cell content leakage

Abrasiveness: The surface of Nano ZnO has been considered to be abrasive due to presence of surface defects such as edges and corners. The bacterial cells are also damaged by the abrasive surfaces of ZnO Nanoparticles.

Source: Royal Society of Chemistry (RSC)

Generation of Reactive Oxygen Species (ROS): Being a semiconductor with a band gap of approx. 3.3 eV, under the influence of radiation with photon energy more than its band gap, leads to the formation of an electron hole (h+). The electron hole (h+) reacts with H2O molecules (from the suspension of ZnO) separating them into •OH and H+. In addition, O2 molecules dissolved in the medium are transformed into superoxide anion radicals (O2˙−), which in turn react with H+ to generate (HO2 •). Subsequently, this species collides with electrons producing hydrogen peroxide anions (HO2−). Thus, the hydrogen peroxide anion reacts with hydrogen ions to produce H2O2 molecules, which is a very strong oxidizing agent. H2O2 molecules elevate the membrane lipid peroxidation that causes membrane leakage of reducing sugars, DNA, Proteins and reduces cell viability.

References

Paula Judith Perez Espitia, Nilda de Fátima Ferreira Soares, Jane Sélia dos Reis Coimbra & Nélio José de Andrade, Renato Souza Cruz, Eber Antonio Alves Medeiros (2012): Zinc Oxide Nanoparticles: Synthesis, Antimicrobial Activity and Food Packaging Applications. Food Bioprocess Technology (2012) 5:1447–1464 Li, J. H., Hong, R. Y., Li, M. Y., Li, H. Z., Zheng, Y., & Ding, J. (2009). Effects of ZnO nanoparticles on the mechanical and antibacterial properties of polyurethane coatings. Progress in Organic Coatings, 64(4), 504–509 Padmavathy, N., & Vijayaraghavan, R. (2008). Enhanced bioactivity of ZnO nanoparticles—an antimicrobial study. Science and Technology of Advanced Materials, 9(3), 035004 Xie, Y., He, Y., Irwin, P. L., Jin, T., & Shi, X. (2011). Antibacterial activity and mechanism of action of zinc oxide nanoparticles against Campylobacter jejuni. Applied and Environmental Microbiology, 77(7), 2325–2331. Zhang, L., Ding, Y., Povey, M., & York, D. (2008). ZnO nanofluids—a potential antibacterial agent. Progress in Natural Science, 18(8), 939–944. Zhang, L., Jiang, Y., Ding, Y., Povey, M., & York, D. (2007). Investigation into the antibacterial behaviour of suspensions of ZnO nanoparticles (ZnO nanofluids). Journal of Nanoparticle Research, 9(3), 479–489. Jones, N., Ray, B., Ranjit, K. T., & Manna, A. C. (2008). Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiology Letters, 279(1), 71–76. Ohira, T., Yamamoto, O., Iida, Y., & Nakagawa, Z. (2008). Antibacterial activity of ZnO powder with crystallographic orientation. Journal of Materials Science. Materials in Medicine, 19(3), 1407–1412 Brayner, R., Ferrari-Iliou, R., Brivois, N., Djediat, S., Benedetti, M. F.,& Fiévet, F. (2006). Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium. Nano Letters, 6(4), 866–870. Adams, L. K., Lyon, D. Y., & Alvarez, P. J. J. (2006). Comparative eco-toxicity of nanoscale TiO2, SiO2, and ZnO water suspensions. Water Research, 40(19), 3527–3532. Emamifar, A., Kadivar, M., Shahedi, M., & Soleimanian-Zad, S. (2010). Evaluation of nanocomposite packaging containing Ag and ZnO on shelf life of fresh orange juice. Innovative Food Science & Emerging Technologies, 11(4), 742–748. Eskandari, M., Haghighi, N., Ahmadi, V., Haghighi, F., & Mohammadi, S. R. (2011). Growth and investigation of antifungal properties of ZnO nanorod arrays on the glass. Physica B: Condensed Matter, 406 (1), 112–114. Gordon, T., Perlstein, B., Houbara, O., Felner, I., Banin, E., & Margel, S. (2011). Synthesis and characterization of zinc/iron oxide composite nanoparticles and their antibacterial properties. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 374 (1–3), 1–8 He, L., Liu, Y., Mustapha, A., & Lin, M. (2011). Antifungal activity of zinc oxide nanoparticles against Botrytis cinerea and Penicillium expansum. Microbiological Research, 166(3), 207–215.

Hirota, K., Sugimoto, M., Kato, M., Tsukagoshi, K., Tanigawa, T., & Sugimoto, H. (2010). Preparation of zinc oxide ceramics with a sustainable antibacterial activity under dark conditions. Ceramics International, 36(2), 497–506. Jalal, R., Goharshadi, E. K., Abareshi, M., Moosavi, M., Yousefi, A., & Nancarrow, P. (2010). ZnO nanofluids: green synthesis, characterization, and antibacterial activity. Materials Chemistry and Physics, 121(1–2), 198–201. Kasemets, K., Ivask, A., Dubourguier, H.-C., & Kahru, A. (2009). Toxicity of nanoparticles of ZnO, CuO and TiO2 to yeast Saccharomyces cerevisiae. Toxicology In Vitro, 23(6), 1116–1122. Bhadra, P., Mitra, M. K., Das, G. C., Dey, R., & Mukherjee, S. (2011). Interaction of chitosan capped ZnO nanorods with Escherichia coli. Materials Science and Engineering: C, 31(5), 929–937. Reddy, K. M., Feris, K., Bell, J., Wingett, D. G., Hanley, C., & Punnoose, A. (2007). Selective toxicity of zinc oxide nanoparticles to prokaryotic and eukaryotic systems. Applied Physics Letters, 90(21), 213902 Sawai, J. (2003). Quantitative evaluation of antibacterial activities of metallic oxide powders (ZnO, MgO and CaO) by conductimetric assay. Journal of Microbiological Methods, 54(2), 177–182. Sawai, J., Shoji, S., Igarashi, H., Hashimoto, A., Kokugan, T., Shimizu, M., & Kojima, H. (1998). Hydrogen peroxide as an antibacterial factor in zinc oxide powder slurry. Journal of Fermentation and Bioengineering, 86(5), 521–522. Roselli, M., Finamore, A., Garaguso, I., Britti, M. S., & Mengheri, E.(2003). Zinc oxide protects cultured enterocytes from the damage induced by Escherichia coli. Journal of Nutrition, 133(12), 4077– 4082. Shi, L., Zhou, J., & Gunasekaran, S. (2008). Low temperature fabrication of ZnO—whey protein isolate nanocomposite. Materials Letters, 62(28), 4383–4385 . Vicentini, D. S., Smania, A., Jr., & Laranjeira, M. C. M. (2010). Chitosan/poly (vinyl alcohol) films containing ZnO nanoparticles and plasticizers. Materials Science and Engineering: C, 30(4), 503–508. Mizieli ´ nska, M.; Łopusiewicz, Ł.; M˛e ˙zy´ nska, M.; Bartkowiak, A. The influence of accelerated UV-A and Q-SUN irradiation on the antimicrobial properties of coatings containing ZnO nanoparticles. Molecules 2017, 22, 1556.

Azizi, S.; Ahmad, M.B.; Hussein, M.Z.; Ibrahim, N.A. Synthesis, Antibacterial and Thermal Studies of Cellulose Nanocrystal Stabilized ZnO-Ag Heterostructure Nanoparticles. Molecules 2013, 18, 6269–6280

Mizieli ´ nska, M.; Lisiecki, S.; Jotko, M.; Chodzy´ nska, I.; Bartkowiak, A. The antimicrobial properties of polylactide films covered with ZnO nanoparticles-containing layers. Przem. Chem. 2015, 94, 1000–1003.

Noshirvani, N.; Ghanbarzadeh, B.; Mokarram, R.R.; Hashemi, M. Novel active packaging based on carboxymethyl cellulose-chitosan-ZnO NPs nanocomposite for increasing the shelf life of bread. Food Packag. Shelf Life 2017, 11, 106–114.

Oprea, A.E.; Pandel, L.M.; Dumitrescu, A.M.; Andronescu, E.; Grumezescu, V.; Chifiriuc, M.C.; Mogoanta, L.; Bal¸seanu, T.-A.; Mogo¸sanu, G.D.; Socol, G.; et al. Bioactive ZnO Coatings Deposited by MAPLE—An Appropriate Strategy to Produce Efficient Anti-Biofilm Surfaces. Molecules 2016, 21, 220.

Silvestre, C.; Duraccio, D.; Marra, A.; Strongone, V.; Cimmino, S. Development of antimicrobial composite films based on isotactic polypropylene and cZnO particles for active food packaging.

oated ZnO particles for active food packaging.

Order online and for more information regarding BioZiny please visit: www.bioziny.com Write to us at: customercare@bioziny.com Call us at: +91 8017644674 Interact with us: Twitter: @bioziny Facebook Page: https://m.facebook.com/BioZiny-105502897591671/ Instagram: @bioziny Manufactured and Marketed by Novonanmek. Manufactured at: Novonanmek Wazirpur, Sector 95 Gurgaon - 122505

www.bioziny.com Write to us at: customercare@bioziny.com