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Rapid, multisample biomolecule isolation & processing
MultiMACS™ M Separator
96-well multisample processing
Sensitive in-column cDNA synthesis
Rapid protein isolation
Easy isolation of viable viruses
Robotic integration
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One instrument for a scope of applications:
Nucleic acid research• mRNAisolationandin-columncDNAsynthesis
Protein research• Purificationofepitope-taggedproteins
• (Co-)Immunoprecipitationofproteins
• Proteinisolationviabiotinylatedcaptureprobes
• In-columnenzymaticreactionsat37°Cor42°C
Virus research• IsolationofinfectiousviruesparticlessuchasHIV-1
Flexibility in magnetic biomolecule isolationOne instrument for manual or fully-automated processes
Figure 2: AutomateduseoftheMultiMACSM96Separatorinapipetrobot
The MultiMACS™ M Separator familyTheMultiMACSMSeparatorfamilyiscomposedoftheMultiMACS M96 SeparatorandMultiMACS M96thermo Separator–instrumentsthateasilyupscaleMiltenyiBiotec’sMACS®Technologyformolecularapplicationstoahigherthroughputformat.Bothinstrumentsperformefficientbiomoleculeisolation,withtheMultiMACSM96thermoSeparatoralsoprovidingpreciseenzymaticreactionsofisolatedmoleculesdirectlyinthecolumnviaaheatablemagnet.
The family at a glance• Smallbenchtopinstruments
• Parallelprocessingofupto96samples
• Manualorroboticoperation(fig.1andfig.2)
• Reliableandconvenientdata
• HeatablemagnetontheMultiMACSM96thermoSeparator allowsenzymaticreactionsofisolatedmolecules
Figure 1: EasymanualoperationoftheMultiMACSM96Separatorviathetouchscreen
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How you benefit from MACS® Technology for biomolecule isolation:
Fast45minutesfor96mRNAsamplesandlessthantwohoursfor96proteinisolations.
Highly sensitiveDuetofastreactionkineticsof50-nmsmallMicroBeads.
High specificityDuetolowrateofbackgroundbindingofMicroBeadsandusageofprovenbindingmoieties.
MACS® Technology for biomolecule isolationTherecognizedstandardincellseparationisappliedtobiomoleculeisolationforfastandsensitiveisolationofmRNA,cDNA,proteinsorviruses.
How MACS Technology worksSuperparamagneticµMACS™MicroBeads(fig.3)areaddedtothecelllysateandinstantlybindtotheirtarget.ThemagneticallylabeledtargetmoleculesareisolatedandpurifiedinMulti-96Columns(fig.4)positionedwithinthemagneticfieldoftheMultiMACSMSeparator.Puretargetmoleculesareelutedafterthoroughwashing.
Figure 3: µMACSAnti-GFPMicroBeads
Figure 4: Multi-96Columnsenableefficientsmall-scaleisolationofbiomoleculeswithMACS®MicroBeads
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One-step mRNA isolation & cDNA synthesisManual or fully-automated 96-well PCR sample preparation at its best
High-purity mRNA from small sample amountsTheMultiMACS™ mRNA Isolation KitsarepreciselydevelopedfortheMultiMACSM96Separatortoprovidescientistswitharobustandreproducible96-wellmRNAisolationprocedurebasedonMACS®Technology.GenomicDNAcontaminationisbelowthelimitofdetection1and96mRNAsamplescanbeprocessedwithinjust45minutes.1)Macket al. (2007)Cytometry71:404–409.
Save time and material – perform cDNA synthesis instantlyInsteadofelutingpurifiedmRNAfromtheMulti-96Columns,first-strandcDNAcanbesynthesizeddirectlyinthesamecolumn.ByemployingtheMultiMACS cDNA Synthesis KitsandtheMultiMACSM96thermoSeparatorwithaheatablemagnet,PCRtemplatescanbepreparedinthesamecolumn.Ittakeslessthan2hourstoobtainpurecDNAfrom96samples.
“We usually process several dozen samples. Now, with the MultiMACS M96thermo Separator we can do parallel cDNA synthesis from up to 96 samples and save a lot of time and costs.”
Prof.C.Wolfrum,ETHZurich,Switzerland
Figure 5: PrincipleofMACSTechnologyformRNAisolationandcDNAsynthesis
CellsortissuearelysedusingLysis/BindingBuffer
AdditionofµMACS™Oligo(dT)MicroBeadstothelysate
PurificationandreleaseofcDNA/mRNAhybrid
ElutionofcDNA/mRNAhybrid
cDNAsynthesismixisappliedontothecolumnandcDNAisgenerated
LysateisappliedtoaMulti-8/96ColumnintheMultiMACS™M96thermoSeparator.WashboundmRNA
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High reliability and reproducibilityTheMultiMACSM96thermoSeparatorandkitsenableexceptionallylowvariationintheyieldandqualityofisolatedmRNAandsynthesizedcDNAasshowninfigures6and7.
Contact-free pipetting eliminates cross-contaminationAhallmarkofMACSTechnologyisthegravity-drivencolumnflow.Thisnotonlycircumventscentrifugationstepsbutalsoenablescontact-freepipettingasthereisnoneedtoremovebuffersafterwashingsteps.Theriskofcross-contaminationofadjacentcolumnsisthusminimized(fig.8).
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-0.051 2 3 4 5 6 7 8 9 10 11 12
1.45–1.60
1.30–1.45
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Figure 8: Contact-freepipettingforreducedriskofcross-contamination
Figure 6: Quantificationof96mRNAsamplespurifiedfrom100μgtotalRNAextractedfrommouseliverusingtheMultiMACSmRNAIsolationKitAverageyield:1.3μgmRNA;coefficientofvariationof96samples:5.2%
1 10 20 30 5040
Cycle number
5 15 25 35 45
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Figure 7: QuantitativeRT-PCRof96samplespurifiedfrom100μgtotalRNAextractedfrommouseliverusingtheMultiMACScDNASynthesisKit.Afterreversetranscription,quantitativeamplificationofthehousekeepinggeneGAPDHwasperformed.Meancyclethreshold:12.0;coefficientofvariation:2.0%
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How you benefit from MACS® Technology for protein isolation:
Time-savingInlessthan2hoursyoucanisolateproteinswithhighpurityfromupto96samplesinasinglerun.
Manual or fully-automated approachesEffortlessroboticintegrationenablespreciseliquidhandlingandfullyautomatedsampleprocessing2.
Reliable dataPreciseandaccuratesamplehandlingensuresreproducibileresultsthatyoucanrelyon.
No cross-contamination Contact-freepipettingensuresthatcross-contaminationiscompletelyeliminated(refertopage5).
FlexibileNumerousreagentkitsareavailableforawideselectionofproteinapplications.
Accelerate your protein researchProtein isolation from 96 samples in less than 2 hours
Manual or automated 96-well isolation of proteinsIncombinationwithoneoftheMultiMACS™ Protein Isolation KitstheMultiMACSM96Separatorcanalsobeusedformanualorautomated96-wellisolationofproteins².
ThemainprincipleofMACS®Technologyforproteinisolationisillustratedinfigure9.²Hubner,Mannet al. (2010)JCB189:739–754.
Figure 9: PrincipleofMACSTechnologyforisolationofepitope-taggedproteins
Lysisofcellsandremovalofcelldebris
LabelingoftargetproteinswithµMACS™Anti-TagMicroBeads
Proteinsandcomplexes
Epitope-taggedprotein
Anti-TagMicroBead
ApplicationoflabeledcelllysatetoMulti-8/96ColumnplacedinaMultiMACSM96Separator
Removalofunboundmaterialbystringentwashing
Optional:enzymaticreactioninthecolumnwiththeMultiMACSM96thermoSeparator
ElutionoftargetproteinwhileMicroBeadsremainincolumn
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“Small magnetic beads in combination with a flow-through column system gave the best results for bait sequence coverage by MS, detection of interaction partners, and robustness while keeping background proteins at acceptable levels.”
“The small beads provide a large surface to volume ratio and consequently favorable binding kinetics as well as short incubation times . . .”
HubnerandMannet. al.(2010)JCB189:739–754.
Figure 10: AutomateduseofMultiMACSM96Separatorinapipetrobot.A:Theroboticarmplacesthewasteplateintherightposition.B:Thepipettingarmappliessamplesandbufferstothecolumns.
Isolate any protein with easeMultiMACS™ProteinIsolationKitsareavailablefor:
Isolation of epitope-tagged proteins Anti-TagMicroBeadsarecoupledtohigh-qualitymonoclonalantibodiesensuringspecificisolationofproteinstaggedwith:
• GFP(greenfluorescentprotein)
• GST(glutathioneS-transferase)
• HA(hemagglutinin)
• His(histidine)
• c-myc
(Co-)Immunoprecipitation of proteins TheextremelysmallMicroBeadsoftheMultiMACS™ProteinA/GKitsensureveryfastreactionkinetics:formationofthelabeledimmunecomplexisgenerallycompletedin30minutes–noneedforovernightincubation.
Chromatin immunoprecipitation (ChIP) ChIPprotocolsalsobenefitfromthehigherspecificityandlowerrateofbackgroundbindingofProteinA/GMicroBeads.
Isolation of proteins via biotinylated capture probes TheMultiMACSStreptavidinKitsefficientlyisolateanymoleculeinteractingwithabiotinylatedcaptureprobe.
A
B
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Standardize 96-well isolation of infectious HIVIsolate infectious HIV virions in less than 1 hour
Rapid magnetic virus isolationIncombinationwiththeMultiMACS™ VitalVirus Isolation Kits,theMultiMACSM96SeparatoracceleratesyourHIVresearch.
YoucanisolateinfectiousHIV-1virionsfromupto96samplesinlessthan1hour.
TheMicroBeadsarecoupledtoamonoclonalantibodyrecognizingCD44onhumanhematopoieticcells.CD44isincorporatedintoHIVparticleswhenthevirusbudsfromthehostcellmembrane.
Infectiousvirionscanbeisolatedwithoutextensivesampleprocessingfromplasma,serum,orotherbodilyfluids,suchas,cerebralspinalfluid,breastmilk,seminalfluid,orcervicallavage.
Figure 11: PrincipleofHIV-1isolationwithMACS®Technology
AdditionofCD44MicroBeadstosupernatant
Removalofproteins,antibodies,andothersamplecomponentsbyrinsingthecolumn
ApplicationofsupernatanttoMulti-96ColumnplacedinaMultiMACSM96Separator
A Elutionofintactvirions
B Lysisofvirusforviralloadassaysorviralantigenimmunoassay
B
A
Centrifugationofsample
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Complete customer careTrusted worldwide customer support from Miltenyi Biotec
Support when and where it’s neededMiltenyiBioteciscommittedtoprovidingoutstandingcustomercareworldwide.
Sales supportAllMiltenyiBiotectechnicalsalesconsultantshaveabackgroundinlifesciencesandcanofferadviceon:
• experimentaldesign
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• e-mailortelephone:simplyasktechnicalsupportaquestion
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Visitwww.miltenyibiotec.com/supportformoredetails.
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Technical specificationsInstrument specifications of the MultiMACS M Separator
MultiMACS M Separator specifications
Size 230×435×230mm
Weight 11kg
Conditionsofoperation 15–30°Cwith0–85%humidityatamaximumaltitudeof2000m
Inputvoltage 100–240VAC,~50/60Hz
Current 3.5Amax.
Powerconsumption 200W
Fuses T3.15AH250V,5×20mm
RS232-Interface(labeled“com”) Pin1,4,6,7,8,9NC;Pin2RXD;Pin3TXD;Pin5GND
DC-Output Module124VDC/8A;Module224VDC/0.315A
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Product Components Order no.
MultiMACS M Separators
MultiMACSM96Separator 130-091-937
MultiMACSM96thermoSeparator 130-094-534
MultiMACS Accessories
Multi-8Columns,molecular(12×8) 12Multi-8Columns,1MultiColumnFrame,1DeepWellBlock,1MicrotiterPlate 130-092-444
Multi-96Columns,molecular(4×96) 4Multi-96ColumnswithMultiColumnFrame,4DeepWellBlocks,4MicrotiterPlate 130-092-445
Multi-8Filters 12Multi-8Filters 130-092-546
Multi-8FiltersandFrames 1Multi-8FilterFrame,12Multi-8Filters 130-092-548
Multi-96Filters 4Multi-96Filters 130-092-547
DeepWellBlock,2.5mL 6DeepWellBlocks,2.5mL 130-092-549
Products Capacity Order no.
Isolation of epitope-tagged proteins
MultiMACSc-mycIsolationKit(12×8)¹ 96isolations 130-094-250
MultiMACSc-mycIsolationKit(4×96)² 384isolations 130-094-251
MultiMACSGFPIsolationKit(12×8)¹ 96isolations 130-094-252
MultiMACSGFPIsolationKit(4×96)² 384isolations 130-094-253
MultiMACSGSTIsolationKit(12×8)¹ 96isolations 130-094-254
MultiMACSGSTIsolationKit(4×96)² 384isolations 130-094-256
MultiMACSHAIsolationKit(12×8)¹ 96isolations 130-094-255
MultiMACSHAIsolationKit(4×96)² 384isolations 130-094-257
MultiMACSHisIsolationKit(12×8)¹ 96isolations 130-094-258
MultiMACSHisIsolationKit(4×96)² 384isolations 130-094-259
(Co)-Immunoprecipitation of proteins
MultiMACSProteinAKit(24×8)³ 192isolations 130-092-944
MultiMACSProteinGKit(24×8)³ 192isolations 130-092-946
MultiMACSProteinAKit(4×96)⁴ 384isolations 130-092-945
MultiMACSProteinGKit(4×96)⁴ 384isolations 130-092-947
Products Capacity Order no.
Protein isolation via biotinylated capture probe
MultiMACSStreptavidinKit(12×8)⁵ 96isolations 130-092-948
MultiMACSStreptavidinKit(4×96)⁶ 384isolations 130-092-949
mRNA isolation and cDNA synthesis
MultiMACSmRNAIsolationKit(12×8)⁷ 96reactions 130-092-520
MultiMACSmRNAIsolationKit(4×96)⁸ 384reactions 130-092-519
MultiMACScDNASynthesisKit(12×8)⁹ 96reactions 130-094-410
MultiMACScDNASynthesisKit(4×96)¹⁰ 384reactions 130-094-408
HIV-1 isolation
MultiMACSVitalVirusHIVIsolation(12×8)11
96isolations 130-092-806
MultiMACSVitalVirusHIVIsolation(4×96)12
384isolations 130-092-807
1Kitcontains3×2mLµMACSAnti-TagMicroBeads,EquilibrationBuffer,12×Multi-8Columns,1MultiColumnFrame,1DeepWellBlock,1MicrotiterPlate.²Kitcontains5×4.6mLµMACSAnti-TagMicroBeads,EquilibrationBuffer,4×Multi-96Columns,4DeepWellBlocks,4MicrotiterPlates.³Kitcontains5×2mLµMACSProteinAorGMicroBeads,24×Multi-8Columns,2MultiColumnFrame,2DeepWellBlock,2MicrotiterPlate.⁴Kitcontains10×2mLμMACSProteinAorGMicroBeads,4Multi-96Columns,4DeepWellBlocks,4MicrotiterPlates.⁵Kitcontains5×2mLμMACSStreptavidinMicroBeads,EquilibrationBuffers,12Multi-8Columns,1MultiColumnFrame,1DeepWellBlock,1MicrotiterPlate.⁶Kitcontains20×2mLμMACSStreptavidinMicroBeads,EquilibrationBuffers,4Multi-96Columns,4DeepWellBlocks,4MicrotiterPlate.⁷Kitcontains3×1mLOligo(dT)MicroBeads,allneededbuffers,12Multi-8Columns,1MultiColumnFrame,1DeepWellBlock,1MicrotiterPlate.⁸Kitcontains4×3×1mLOligo(dT)MicroBeads,allneededbuffers,4Multi-96Columns,4DeepWellBlocks,4MicrotiterPlates.⁹Kitcontains3×1mLOligo(dT)MicroBeads,96cDNAsynthesisenzymmixes,allneededbuffers,12Multi-8Columns,1MultiColumnFrame,1DeepWellBlock,1MicrotiterPlate.¹⁰Kitcontains4×3×1mLOligo(dT)MicroBeads,4×96cDNAsynthesisenzymemixes,allneededbuffers,4Multi-96Columns,4DeepWellBlocks,4MicrotiterPlates.¹¹Kitcontains5×µMACSVitalVirusHIVIsolationReagents,12Multi-8Columns,1DeepWellBlock,1MicrotiterPlate.¹²Kitcontains20×µMACSVitalVirusHIVIsolationReagents,4Multi-96Columns,4DeepWellBlocks,4MicrotiterPlates.
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Unlessotherwisespecificallyindicated,MiltenyiBiotecproductsandservicesareforresearchuseonlyandnotfortherapeuticordiagnosticuse.MACS,MultiMACS,andµMACSareregisteredtrademarksortrademarksofMiltenyiBiotecGmbH.Copyright©2013MiltenyiBiotecGmbH.Allrightsreserved.
