Embed Size (px)
DESCRIPTION
jurnal
Citation preview
1. Joko Ariswanto 125040200111033
2. Eva Muthahara 125040200111128
3. Imtikhanna D. W. 125040201111053
4. Febrina Dwi Hapsari 125040201111093
5. M. Bayu Mario 125040201111238
GROUP 5
Effect of Gamma Radiation On Callus Induction And Regeneration of Rosa
canina Through Invitro Culture
Trakia Journal of Sciences2013Volume 11 No. 2Page 158-162
S. MoallemM. Behbahani
S. Mousavi
Bulgaria
Sinar gamma = Sinar kuat sebagai MUTAGEN
Ketidakstabilan inti sel Menginduksi perubahan genetik
Dosis iradiasi diukur dalam satuan Gray (Gy), 1 Gy sama dengan 0.10 krad yakni 1 J energy per kilogram
iradiasi yang dihasilkan. Dosis iradiasi dibagi tiga, yaitu tinggi (>10 k Gy), sedang (1-10 k Gy), dan
rendah (< 1 k Gy).
Tahun 1900 oleh Paul Ulrich Villard
KALUS
Materials And MethodesSterilization of Leaf Explants
Aquades
Aquades
Aquades
Ethanol 70 %40 detik
Sodium hypochlorite
2,5%
Leaf explants
Materials And MethodesProvision of Medium
B5 MSSukrosa30 g/l
Agar7 g/l
Autoclave 121 oC1,05 kg/cm 15 Menit
HCl & NaOH0,1 N
Botol Kultur@ 20 ml
Materials And MethodesCallus Induction
B5
0,5mg/l
IAA
NAA
IAA
NAA
IAA
NAA
1mg/l
2mg/l
Light Condition1200 Lux
Dark Condition
B5
0,5mg/l
IAA
NAA
IAA
NAA
IAA
NAA
1mg/l
2mg/l
Materials And MethodesCallus Induction
MS
0,5mg/l
IAA
NAA
IAA
NAA
IAA
NAA
1mg/l
2mg/l
Light Condition1200 LuxMS
0,5mg/l
IAA
NAA
IAA
NAA
IAA
NAA
1mg/l
2mg/l
Dark Condition
Materials And MethodesIndirect Regeneration
MS
1,5 mg/l BAP
0,5 mg/l NAA
1,5 mg/l BAP
0,5 mg/l IAA
1,5 mg/l BAP
0,5 mg/l GA3
1,5 mg/l KIN
0,5 mg/l NAA
1,5 mg/l KIN
1,5 mg/l KIN
0,5 mg/l IAA
0,5 mg/l GA3
Results
Results
1. The effect of differences hormonal
and dark and light treatments on callus
induction of leaf explants:
The leaf explants were transferred to medium, produced phenol, Browning was observed around leaf explants.
The explants did not promote any callus in light conditions. Callus induction was only seemed in dark condition. Greenish yellow callus was friable.
These results showed that the highest callus induction per explants was obtained after 7-23 days in MS-medium in contrast to B5 within 6-15 days.
Results
2. The effect of different hormone on
indirectregeneration:
In the medium containing 1.5 mg/l BAP with 0.5 mg/l NAA and medium 1.5 g/l BAP with 0.5 mg/l IAA, the size of calluses did not change and no organogenesis was performed during 4 week.In the medium containing 1.5 mg/l KIN with 0.5 mg/l NAA and 1.5 mg/l KIN with 0.5 mg/l IAA, although size of calluses increased gradually from beginning of the first week, but no organogenesis occurred as well.After 1 week of cultures, medium containing 1.5 mg/l BAP in combination with 0.5 mg/l GA3 and also the medium of 1.5 mg/l KIN with 0.5 mg/l GA3 necroses was occurred. At the end of the second week, they turned brown
Results
3. The effect of radiation on callus:
It appeared that no callus change in radiation of various Gama ray doses and no organogenesis is occurred, at the end of the 6th week, callus necrosesed.
Conclusion
This study indicated callus induction in Rosa canina. Different concentration of IAA and NAA under dark and light condition with composition of culture medium were tested. Significant increase obtained in B5 medium containing 1 mg/l IAA under the dark condition. In indirect regeneration no result, observed in medium containing in MS medium supplemented with KIN in combination with NAA, IAA, GA3 or BAP in combination with NAA, IAA, and GA3. When Gama raying used, there was not effective on callus induction positively.
