PHARMACY Major STUDY PROGRAM 2012/2013 Subjects of the …aok.pte.hu/.../tantervek_2012/course_eng_pha_basic_1213.pdf · 2012-08-13 · Medical School PHARMACY Major STUDY PROGRAM

UP MS Pharmacy major – subjects of the Basic module – academic year of 2012/2013

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The University of Pécs Medical School

PHARMACY Major

STUDY PROGRAM 2012/2013

Subjects of the Basic module

(Obligatory subjects)


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OPAAN1 ANALYTICAL CHEMISTRY 1

Course director: DR. IMRE HUBER, research professor (junior) Department of Pharmaceutic Chemistry

5 credit ▪ semester exam ▪ Basic module ▪ autumn semester ▪ recommended semester: 1

Number of hours/semester: 28 lectures + 42 practices + 0 seminars = total of 70 hours Headcount limitations (min-max.): min. 1 – max. 20

Prerequisites: -

Topic Within the frame of the theoretical and practical trainings of this subject students will study the analytical approach of chemistry. This approach is crucial for the study of almost all pharmaceutical sciences like pharmaceutical chemistry, pharmaceutical technology etc. Students have to learn and examine the theory and practice of analytical reactions, methods, rules and calculations. Students will learn how to identify an unknown inorganic sample in both simple and complex manner of the analysis. At the end of the semester they should be able to know how to analyze an unknown sample for the most important cations and anions. Students should prove to demonstrate that they know how to use the theoretical knowledge in the pharmaceutical practice while finding out what their unknown sample is.

Conditions for acceptance of the semester Exam topics/questions The semester is closed with a written test (maximum score: 40 points). In the case the result will not reach the 50% level, the student fails, she or he has to repeat the exam. In all other cases (above 50%), the student will receive a grade from qualitative inorganic analysis, based on the results of the two written tests (maximum score: 40 points) and the overall result of the practical work throughout the semester (maximum score: 5 points). The first retake exam is also a written test. The second one is an oral examination. The method of determination of grade is the same in all the three cases.

Making up for missed classes All missed practicals are to be recovered on the next meeting (next week).

Reading material 1. P.W. West, M.M. Vieck, A.L. LeRosen: Qualitative analysis and analytical chemical separations 2. H F. Holtzclaw, W. R. Robinson: College Chemistry with Qualitattive Analysis 3. A. Lásztity, J. Gyimesi: Qualitative Inorganic Analysis

Lectures 1 Definition, principles Dr. Huber Imre 2 Topic of qualitative inorganic analysis Dr. Huber Imre 3 Equilibrium reactions in solution Dr. Huber Imre 4 Definition, calculations Dr. Huber Imre 5 Classification of chemical reactions Dr. Huber Imre 6 Electrode potentials Dr. Huber Imre 7 Sensitivity and Dr. Huber Imre 8 Specificity of a chemical reaction Dr. Huber Imre 9 Classification of the cations Dr. Huber Imre 10 1st group of cations Dr. Huber Imre 11 2nd group of cations Dr. Huber Imre 12 Separation of the first two groups Dr. Huber Imre 13 3rd group of cations I. Dr. Huber Imre 14 3rd group of cations II. Dr. Huber Imre


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15 3rd group of cations III. Dr. Huber Imre 16 Separation of the 3rd group Dr. Huber Imre 17 4th group of cations Dr. Huber Imre 18 5th group of cations Dr. Huber Imre 19 Classification of anions Dr. Huber Imre 20 1st anion group Dr. Huber Imre 21 2nd anion group I. Dr. Huber Imre 22 2nd anion group I. Dr. Huber Imre 23 3rd anion group Dr. Huber Imre 24 3rd anion group II. Dr. Huber Imre 25 4th anion group I. Dr. Huber Imre 26 4th anion group II. Dr. Huber Imre 27 Final written test Dr. Huber Imre 28 Summary Dr. Huber Imre

Practices 1 Laboratory regulations 2 Safety instructions 3 Laboratory equipments 4 Silver, Lead 5 Mercury(I) and (II) 6 Copper, Cadmium, Bismuth 7 Arsenic 8 Antimony 9 Tin 10 Nickel, Cobalt, Iron(II) 11 Iron(III), Manganese 12 Chromium 13 Written test 14 Aluminum 15 Zinc 16 Simple analysis of the 1st group 17 Simple analysis of the 2nd group 18 Simple analysis of the 3rd group 19 Calcium 20 Strontium 21 Barium 22 Magnesium, Lithium 23 Sodium, Potassium 24 Ammonium 25 Written test II. 26 Simple analysis of the 4th group 27 Simple analysis of the 4th group 28 Carbonate, Hydrocarbonate 29 Sulphite, Thiosulphate 30 Polysulphide, Silicate


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31 Hypochloride, Iodate 32 Bromate, Sulfate 33 Phosphates 34 Borate, Fluoride 35 Chloride, Bromide 36 Iodode, Cyanide 37 Thiocyanide 38 Nitrite, Nitrate, Chlorate, Perchlorate, Acetate 39 Written test III. 40 Complex 41 Complex 42 Complex

Seminars

Exam topics/questions 1.) Definition, principles and topic of qualitative inorganic analysis. Quality assurance and control of chemical substances and

active pharmaceutical ingredients. Available reactions. Stoichiometry. 2. Equilibrium reactions in solution, definition, calculations. Acid-base theories, calculation of pH, complexes (steric structures

and isomerism), precipitate formation, solubility. 3.) Classification of chemical reactions: acid-base, complex-forming, and redox reactions. Electrode potentials. 4.) Sensitivity and specificity of chemical reactions. Dilution limit, limit-concentration. Analytical equipments, methods (macro,

micro, semimicro, etc). Preliminary investigations: sample-taking, homogenization, dissolution, digestion, direct heating, flame-test, etc.

5.) Classification of the cations: 1st group of cations (Ag+, Pb2+, Hg22+, Hg2+, Cu2+, Cd2+, Bi3+). 6.) 2nd group of cations (As3+, As5+, Sb3+, Sb5+, Sn2+, Sn4+. Separation of the first two groups of cations. 7.) 3rd group of cations I. (Co2+, Ni2+, Fe2+, Fe3+, Cr3+, Mn2+, Al3+, Zn2+). 8.) 3rd group of cations II. (Co2+, Ni2+, Fe2+, Fe3+, Cr3+, Mn2+, Al3+, Zn2+). Separation of the 3rd group of cations. 9.) 4th and 5th group of cations (Ca2+, Sr2+, Ba2+; Mg2+, Li+, Na+, K+, NH4+). Separation of the 4th and 5th group.

Separation of magnesium ion from the other ions of the 5th group. 10.) Classification of the anions: 1st group of anions (CO32-, HCO3-, SO32-, S2O32-, S2- and Sx2-, SiO32-, OCl-). 11.) 2nd group of anions (IO3-, BrO3-, SO42-, PO43-, B(OH)4-, F-). 12.) 3rd group of anions (Cl-, Br-, I-, CN-, SCN-). 13.) 4th group of anions (NO2-, NO3-, ClO3-, ClO4-, CH3COO-). Complex analysis: cation(s) and anion(s) in the same

sample.Practices 1.) Laboratory regulations, safety, protection against accidents, notebook (keeping and recording), laboratory equipments and

working place. 2.) Investigation of the reactions of silver, lead, mercury(I), mercury(II), copper, cadmium and bismuth ions. 3.) Reactions with arsenic(III) and arsenic(V), antimony(III) and antimony(V), tin(II) and tin(IV) ions. 4.) Study on the reactions of nickel, cobalt, iron(II) and iron(III), manganese, chromium ions. 5.) 1st written test. Reactions of aluminum and zinc ions. 6.) Simple analysis of the 1st, 2nd and 3rd group of cations. 7.) Reactions with calcium, barium and strontium ions. 8.) Exercise on the reactions of magnesium, lithium, sodium, potassium and ammonium ions. 9.) 2nd written test. Simple analysis of the 1st, 2nd, 3rd, 4th and 5th group of cations. 10.) Investigation of the anions: carbonate, hydrocarbonate, sulphite, thiosulphate, sulphide, polisulphide, silicate, hypochloride,

iodate, bromate, and sulphate ions. 11.) Study of the reactions of phosphate, borate, fluoride, chloride, bromide, iodide, cyanide and thiocyanide ions. 12.) Experiments on the reactions of nitrite, nitrate, chlorate, perchlorate, and acetate ions. Simple analysis of the anions. 13.) 3rd written test. Simple analysis of the anions. Complex analysis. Stock-taking.

Participants Dr. Huber Imre (HUIRAAO.PTE)


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OPAAN2 ANALYTICAL CHEMISTRY 2

Course director: DR. PÁL PERJÉSI, professor Department of Pharmaceutic Chemistry

5 credit ▪ final exam ▪ Basic module ▪ spring semester ▪ recommended semester: 2


Prerequisites: OPAAN1 completed + OPAAT1 completed

Topic Within the frame of the theoretical and practical trainings of this subject students will study the analytical approach of chemistry. This approach is crucial for the study of almost all pharmaceutical sciences like pharmaceutical chemistry, pharmaceutical technology etc. Students have to learn and examine the theory and practice of analytical reactions, methods, rules and calculations. Students will learn how to determine a sample with unknown concentration in both simple and complex manner of the analysis. At the end of the semester they should be able to know how to analyze with the most important quantitative methods of the quantitative analysis.

Conditions for acceptance of the semester The participation in both the lectures and the practices of the course is obligatory. Maximum 3 absences from lectures and practices are allowed. 80% of the performed quantitative determinations should be accepted. During the course two tests are required. Both semester tests results should be above 50%. Missed practices could be made up during the make-up opportunities announced by the department.

Making up for missed classes Missed practices could be made up during the make-up opportunities announced by the department. During each make-up session only one missed practice can be executed.

Reading material Daniel C. Harris: „Quantitative Chemical Analysis”, W.H. Freeman and Co., New York, 1998, 5th ed.

Lectures 1 Introduction to quantitative chemical analysis. The analytical process. Dr. Perjési Pál 2 Introduction to quantitative chemical analysis. The analytical process. Dr. Perjési Pál 3 The volumetric analysis. Types of reactions. Experimental error. Dr. Perjési Pál 4 The volumetric analysis. Types of reactions. Experimental error. Dr. Perjési Pál 5 Acid-base equilibria. Acid-base titrations. Dr. Perjési Pál 6 Acid-base equilibria. Acid-base titrations. Dr. Perjési Pál 7 Neutralization analysis. Titration curve. Indication of endpoint. Dr. Perjési Pál 8 Neutralization analysis. Titration curve. Indication of endpoint. Dr. Perjési Pál 9 Neutralization analysis. Titrations in non-aqueous solutions. Dr. Perjési Pál 10 Neutralization analysis. Titrations in non-aqueous solutions. Dr. Perjési Pál 11 Complexometry I. Test Paper I. Dr. Perjési Pál 12 Complexometry I. Test Paper I. Dr. Perjési Pál 13 Complexometry II. Dr. Perjési Pál 14 Complexometry II. Dr. Perjési Pál 15 Redox titrations. Titration curve. Indication of endpoint. Dr. Perjési Pál 16 Redox titrations. Titration curve. Indication of endpoint. Dr. Perjési Pál


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17 Redox titrations. Oxidimetry I. Dr. Perjési Pál 18 Redox titrations. Oxidimetry I. Dr. Perjési Pál 19 Redox titrations. Oxidimetry II. Dr. Perjési Pál 20 Redox titrations. Oxidimetry II. Dr. Perjési Pál 21 Redox titrations. Reductometry. Dr. Perjési Pál 22 Redox titrations. Reductometry. Dr. Perjési Pál 23 Precipitate formation titrations. Dr. Huber Imre 24 Precipitate formation titrations. Dr. Huber Imre 25 Gravimetry I. Test Paper II. Dr. Huber Imre 26 Gravimetry I. Test Paper II. Dr. Huber Imre 27 Gravimetry II. Dr. Huber Imre 28 Gravimetry II. Dr. Huber Imre

Practices 1 Acceptance of lab equipment, stock-taking, fire prevention and safety education. 2 General Introduction: Burette calibration. 3 General Introduction: Pipette calibration. 4 Factor for 0.1 M hydrochloric acid measuring solution. 5 Factor for 0.1 M sodium hydroxide measuring solution. 6 Factor for 0.1 M sodium hydroxide measuring solution. 7 Acid-base titrations I. 8 Titration of a weak acid (acetic acid or propionic acid) using sodium hydroxide. 9 Determination of borax (sodium tetraborate) by acidimetry. 10 Acid-base titrations II. 11 Indirect determination of sodium thiosulfate by alkalimetry. 12 Parallel determination of sodium hydroxide and sodium carbonate by Winkler’s method. 13 Acid-base titrations III. - Non-aqueous titrations. 14 Determination of potassium acetate in glacial acetic medium. 15 Determination of potassium acetate in glacial acetic medium. 16 Complexometry I. 17 Measurements based on the formation of highly stable complexes. - Determination of mercury(II) using thiocyanate

measuring solution by Volhard’s method. 18 Direct chelatometric titrations. - Determination of nickel(II). 19 Complexometry II. 20 Indirect chelatometric titrations, measurement of the excess of measuring solution. - Determination of sulphate ions. 21 Complex chelatometry.- Parallel determination of calcium and magnesium ions. 22 Redox titrations I.- Permanganometry. 23 Factor for 0.1 N (0.02 M) potassium permanganate measuring solution. 24 Determination of iron(II) by permanganate titration. 25 Redox titrations II. - Permanganometry. 26 Determination of hydrogen peroxide by permanganate titration. 27 Permanganometric determination of potassium bromide by Winkler’s method. 28 Redox titrations III. - Bromatometry. 29 Bromometric determination of antimony(III). 30 Determination of formic acid by bromatometry. 31 Redox titrations IV.- Iodometry. 32 Factor for 0.01 N (0.01 M) sodium thiosulfate measuring solution. 33 Determination of potassium iodide by Winkler’s method.


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34 Precipitate formation titrations. - Argentometry. 35 Chloride ion determination by Volhard’s method. 36 Iodide ion determination by Pungor’s and Schulek’s method. 37 Gravimetry I. 38 Determination of crystallization water in magnesium sulfate heptahydrate. 39 Determination of magnesium ion in form of MgNH4PO4 x 6 H2O. 40 Gravimetry II. 41 Determination of barium ion in form of barium sulfate. 42 Determination of phosphate ion in form of MgNH4PO4 x 6 H2O.

Seminars

Exam topics/questions Shows the test questions are the presentation themes.

Participants Dr. Almási Attila (ALAFAEO.PTE), Dr. Kuzma Mónika (KUMFABO.PTE)


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OPAAT1 GENERAL AND INORGANIC CHEMISTRY 1




Prerequisites: -

Topic The course includes the topics of General Chemistry that are essential for pharmacy students to study the chemistry-related subjects in the higher semesters.

Conditions for acceptance of the semester Acknowledgement of the course is in accord with the Educational Bye-law of the University. Two tests will be written during the semester. The result of both tests should be above 50%. Grades are based on the results of the tests.

Making up for missed classes Missed practices could be made up during the make-up opportunities announced by the department. During each make-up session only one missed practice can be executed.

Reading material J. Mc Murray, R. C. Fay: Chemistry (5th edition) Darrell D. Ebbing (Ed.): General Chemistry (latest edition)

Lectures 1 History of Chemistry. Classification of matter. Structure of atoms. Periodic table. Periodic properties. Dr. Perjési Pál 2 History of Chemistry. Classification of matter. Structure of atoms. Periodic table. Periodic properties. Dr. Perjési Pál 3 Structure of molecules. Chemical bonds. The valence bond theory. Hybriditzation of orbitals. Molecular geometry, the

VSEPR theory. Dr. Molnár Péter 4 Structure of molecules. Chemical bonds. The valence bond theory. Hybriditzation of orbitals. Molecular geometry, the

VSEPR theory. Dr. Molnár Péter 5 The states of matter. The gaseous state. Weak bonding forces. The liquid state. The solid state. Phase diagrams. Dr. Molnár Péter 6 The states of matter. The gaseous state. Weak bonding forces. The liquid state. The solid state. Phase diagrams. Dr. Molnár Péter 7 Water and the aqueous solutions. Dissolution of gases, liquids and solids in liquids. Electrolytes. Dr. Molnár Péter 8 Water and the aqueous solutions. Dissolution of gases, liquids and solids in liquids. Electrolytes. Dr. Molnár Péter 9 Colligative properties. Colloids. Dr. Perjési Pál 10 Colligative properties. Colloids. Dr. Perjési Pál 11 Thermochemistry. Hess’s law. Laws of thermodinamics. Dr. Perjési Pál 12 Thermochemistry. Hess’s law. Laws of thermodinamics. Dr. Perjési Pál 13 Chemical kinetics. Dr. Molnár Péter 14 Chemical kinetics. Dr. Molnár Péter 15 Chemical equilibria, the law of mass action. Protolytic reactions. The pH and its calculation. Dr. Lóránd Tamás 16 Chemical equilibria, the law of mass action. Protolytic reactions. The pH and its calculation. Dr. Lóránd Tamás 17 Protolytic reactions. Acid/base equilibria. Strength of acids and bases. Dr. Lóránd Tamás


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18 Protolytic reactions. Acid/base equilibria. Strength of acids and bases. Dr. Lóránd Tamás 19 Hydrolysis of salts. Buffer systems. Acid-base indicators. Dr. Lóránd Tamás 20 Hydrolysis of salts. Buffer systems. Acid-base indicators. Dr. Lóránd Tamás 21 Heterogeneous equilibria. The solubility product constant, solubility. Dr. Perjési Pál 22 Heterogeneous equilibria. The solubility product constant, solubility. Dr. Perjési Pál 23 Electrochemistry I. Dr. Perjési Pál 24 Electrochemistry I. Dr. Perjési Pál 25 Electrochemistry II. Dr. Perjési Pál 26 Electrochemistry II. Dr. Perjési Pál 27 Structure, geometry and stability of complex es. Types of ligands. Dr. Perjési Pál 28 Structure, geometry and stability of complex es. Types of ligands. Dr. Perjési Pál

Practices 1 Laboratory safety. Introduction and handover of laboratory equipments. Basic principles. Classification of matter.

Naming simple compounds: Acids, bases and salts. Weighing. 4 Basic principles of calculations I: Concentrations. Delivering liquids. Preparation of solutions. Measuring density. 7 Basic principles of calculations II: Concentrations. Purification of inorganic compounds I.: Decantation, Filtration.

Recrystallisation. Purification of alum by recrystallisation I, Dilution of sulutions. 10 Basic principles of calculations III: Concentartions. Purification of inorganic compounds II.: Destillation, Sublimation. 13 Basic principles of calculations III: Stochiometry. Purification of inorganic compounds III. Desalination of water.

Extraction. 16 Basic principles of calculations IV. Stochiometry. Basic thermodinamics. Hess’s law. Observation of thermal

decompositions. Determination of melting point. Determination 19 Basic principles of chemical kinetics. Observation of reaction rates. Landolt-reaction. Oscillating reactions. 22 Electrolytic dissociation. Weak and strong electrolytes. Preparation of boric acid from borax I., Preparation of

potassium dihydrogenphosphate I. 25 Acid-base equilibrium I. Arrhenius concept, Brønsted-Lowry concept, Lewis concept. 28 Acid-base equilibrium II. Hydrolysis of ions. Buffers. Observation of hydrolysis of salts Demonstation of buffer

capacity. 31 Acid-base equilibrium III. Acid-base titrations Determination of concentration of monoprotic acid solutions

(hydrochloric acid, acetic acid) by titration. 34 Heterogenous equilibrium. Solubility calculations. Qualitative comparison of solubility products. 37 Redox reactions I. Oxidation state. Important oxidizing and reducing agents. Observation of oxidation-reduction

reactions. Preparation of copper(I) oxide through copper(I) chloride 40 Redox reactions II. Electrodes, electrochemical cells, electrolysis. Redox titrations

Seminars

Exam topics/questions Written exam covering the topics of the lectures and the laboratory practices. Further details: www.gytsz.pte.hu

Participants Dr. Almási Attila (ALAFAEO.PTE)


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OPAAZ2 GENERAL AND INORGANIC CHEMISTRY 2




Prerequisites: OPAAT1 completed

Topic This subject is based on the acquired theoretical knowledge on General Chemistry, with adaptation of the principles to be able to understand physical and chemical properties of the most important pharmacy-related elements and inorganic compounds.

Conditions for acceptance of the semester Acknowledgement of the course is in accord with the Educational Bye-law of the University. Two tests will be written during the semester. The result of both tests should be above 50%. Evaluation of semester performance is based on the results of all the written tests.

Making up for missed classes

Reading material J. McMurray, R. C. Fay: Chemistry (5th edition) Darrell D. Ebbing (Ed.): General Chemistry (latest edition)

Lectures 1 Classification of elements. Elements and compounds. Nomenclature of inorganic compounds. Dr. Perjési Pál 2 -II- Dr. Perjési Pál 3 Hydrogen and hydrides. Noble gases. Dr. Huber Imre 4 -II- Dr. Huber Imre 5 Halogens. Halogenids. Dr. Huber Imre 6 -II- Dr. Huber Imre 7 Oxygen and oxygen compounds. Dr. Huber Imre 8 -II- Dr. Huber Imre 9 Sulfur and sulfur compounds. Dr. Huber Imre 10 -II- Dr. Huber Imre 11 Nitrogen and nitrogen compounds. Dr. Huber Imre 12 -II- Dr. Huber Imre 13 Phosphorus and phosphorus compounds. Test I. Dr. Huber Imre 14 -II- Dr. Huber Imre 15 Arsenic, bismuth and their compounds. Carbon and carbon compounds. Dr. Perjési Pál 16 -II- Dr. Perjési Pál 17 Silicon and silicon compounds. Dr. Perjési Pál 18 -II- Dr. Perjési Pál


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19 Boron and aluminium compounds. The alkali metals and their compounds. Dr. Perjési Pál 20 -II- Dr. Perjési Pál 21 The alkaline earth metals and their compounds. Transition metals. Dr. Perjési Pál 22 -II- Dr. Perjési Pál 23 The structure of complexes. Manganase and manganase compounds. Dr. Perjési Pál 24 -II- Dr. Perjési Pál 25 Iron and iron compounds. Test II. Dr. Perjési Pál 26 -II- Dr. Perjési Pál 27 Copper, silver and their compounds. Zinc, cadmium mercury and their compounds. Dr. Perjési Pál 28 -II- Dr. Perjési Pál

Practices

Seminars 1 The periodic table. Periodic properties 2 The gaseous state. Kinetic theory of gases. Thermodynamic parameters, state functions. 3 Basics of thermodynamics. Internal energy and enthalpy. Entropy. 4 Chemical kinetics. Rate of reactions and reaction order. Temperature dependence of the reaction rate. 5 Homogeneous and heterogeneous chemical equilibria. Equilibrium constant. Le Chateleir principle. 6 Free energy change of chemical reactions. Thermodynamic requirements of spontaneous chemical reactions. 7 Conductivity of electrolytes. Strong and weak electrolytes. 8 Acid-base theories. (Arrhenius, Bronsted-Lowry, Lewis, Pearson) 9 Formation and stability of complexes. Theories of complex formation. 10 pH of aqueous solutions I. Hydrolysis of salts. The hydrolysis constant. 11 pH of aqueous solutions II. Buffers. Buffer capacity. 12 Galvanic cells. Electrode potential. Electrodes of first and second kind. 13 Redox potential. Thermodynamic requirements of spontaneous redox reactions. 14 Electrolysis. Decomposition voltage. Polarization.

Exam topics/questions Oral exam covering the topics of the subjects General and Inorganic Chemistry I and II. Before the exam each student should have a Minimum Requirent Test of which result should be at least 80%. Further details: www.gytsz.pte.hu

Participants Dr. Almási Attila (ALAFAEO.PTE), Dr. Kuzma Mónika (KUMFABO.PTE)


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OPABM1 BIOMATHEMATICS 1

Course director: DR. LÁSZLÓ GRAMA , assistant professor Department of Biophysics



Prerequisites: -

Topic Introduction into fundamentals and methods of mathematical analysis and biostatistics. Applications in the fields of physics, chemistry and biology. This course focuses on the acquisition of the basic knowledge of mathematics and special courses will introduce the special applications. Topics discussed during the course: Definition, type and discussion of the functions. Derivatives of elementary functions, geometrical interpretation, differentiation rules and applications. Integration. Solving basic integral exercises and differential equations. Examples from physics, chemistry and biology. Interpretation of multivariable functions, finding partial derivatives and extreme values. Fitting set of points by least squares method. Practical examples for solving differential equations.

Conditions for acceptance of the semester

Making up for missed classes consultation

Reading material Belagyi, Mátyus, Nyitrai: Matematics, University of Pécs, 2010 Strang, G., Calculus, Wellesley-Cambridge Press, Wellesley, USA,1991 Olive, J. : Maths, A Student’s Survival Guide, Cambridge University, 2003

Lectures 1 Fundamental idea and operations of set theory. Sets of numbers. Definitions, properties and types of functions.

Elementary and complex functions. Function transformations Dr. Grama László 2 Continue Dr. Grama László 3 Definition for the limit of sequence and function. Limit and continuity of elementary functions. Dr. Grama László 4 Continue Dr. Grama László 5 Definition of the difference quotient and the derivative of function. Interpretation through geometrical and physical

examples. Derivatives of elementary functions. Dr. Grama László 6 Continue Dr. Grama László 7 Differentiation rules. Dr. Grama László 8 Continue Dr. Grama László 9 Applications of the derivative. Function analysis. L’Hopital’s rule Dr. Grama László 10 Continue Dr. Grama László 11 Approximation of a function: Taylor polynomials, Taylor series. Power series. Dr. Grama László 12 Continue Dr. Grama László 13 Multivariable functions: graph, partial derivatives and extreme values. Fitting set of points by least squares method. Dr. Grama László 14 Continue Dr. Grama László 15 Indefinite integral. Integrals of elementary functions. Rules for integrals: integration by parts and by substitution. Dr. Bugyi Beáta 16 Continue Dr. Bugyi Beáta


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17 Definite integral. The definite integral as the net signed area of the region bounded by its graph. Estimation of the definite integral.

Dr. Bugyi Beáta 18 Continue Dr. Bugyi Beáta 19 Applications of the integral: finding the area under a curve. Motion. Length of a plane curve. Dr. Bugyi Beáta 20 Continue Dr. Bugyi Beáta 21 Differential equations in general, types of the differential equations. Separable differential equations. Dr. Bugyi Beáta 22 Continue Dr. Bugyi Beáta 23 Solving first order linear differential equations. Dr. Bugyi Beáta 24 Continue Dr. Bugyi Beáta 25 Practical examples for differential equations (1): chemical reactions, catalysts and enzymes. Dr. Bugyi Beáta 26 Continue Dr. Bugyi Beáta 27 Practical examples for differential equations (2): proliferation, decay and elimination processes; pharmacokinetic

investigations; compartment models Dr. Bugyi Beáta 28 Continue Dr. Bugyi Beáta

Practices 1 Refreshing knowledge from the secondary school 2 Continue 3 exercises from the topics of the lecture 4 Continue 5 exercises from the topics of the lecture 6 Continue 7 exercises from the topics of the lecture 8 Continue 9 exercises from the topics of the lecture 10 Continue 11 TEST 1 exercise solution 12 Continue 13 exercises from the topics of the lecture 14 Continue 15 exercises from the topics of the lecture 16 Continue 17 exercises from the topics of the lecture 18 Continue 19 exercises from the topics of the lecture 20 Continue 21 exercises from the topics of the lecture 22 Continue 23 exercises from the topics of the lecture. 24 Continue 25 TEST 2 exercise solution 26 Continue 27 exercises from the topics of the semester 28 Continue

Seminars


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Exam topics/questions Solution of problems: differentiation and integration of functions, solution of simple differential equations

Participants Dr. Bugyi Beáta (BUBEAB.T.JPTE), Dr. Grama László (GRLHAAO.PTE)


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OPABM2 BIOMATHEMATICS 2

Course director: DR. LÁSZLÓ GRAMA , assistant professor Department of Biophysics

4 credit ▪ semester exam ▪ Basic module ▪ spring semester ▪ recommended semester: 2


Prerequisites: -

Topic Basic data handling and computer use. Exploring data by graphical and numerical characterisation. Basic concepts of probability and statistical inference. The basic methods for statistical inference most frequently used in medicine.

Conditions for acceptance of the semester Two written tests. A problem to be solved by the computer and a theory question.

Making up for missed classes extra class

Reading material Official textbook: Belágyi: Medical biometry, Pécs, 2011 Other textbooks: 1, Moore, D. S. The Basic Practice of Statistics, 5th Ed., 2010. 2, Moore, David S., McCabe, George P. Introduction to the Practice of Statistics 5th Ed, 2005, W.H. Freeman,Yates, Dan, Moore, David S., Starnes, Daren S. The Practice of Statistics (TI-83/89 Graphing Calculator Enhanced) 2/e, 2003, W.H. Freeman 3. Rees, W. G. Essential Statistics, Chapman and Hall, 1992

Lectures 1 Introduction (Statistics in medicine, models). Probability. Dr. Bódis Emőke 2 Continue Dr. Bódis Emőke 3 Variables, Discrete distributions (binomial and Poisson). Dr. Bódis Emőke 4 Continue Dr. Bódis Emőke 5 Continuous variables. Histogram, relative frequency density and probability density function. Dr. Bódis Emőke 6 Continue Dr. Bódis Emőke 7 Mean and standard deviation. The normal distribution. Dr. Bódis Emőke 8 Continue Dr. Bódis Emőke 9 Distribution of the sample mean, standard error. Dr. Bódis Emőke 10 Continue Dr. Bódis Emőke 11 Confidence interval for the expected value. The t distribution Dr. Bódis Emőke 12 Continue Dr. Bódis Emőke 13 Principle of hypothesis testing. The one sample and the paired samples t tests. The sign test (preview). Dr. Bódis Emőke 14 Continue Dr. Bódis Emőke 15 The confidence interval and the hypothesis testing. Type I and type II errors. Dr. Visegrády Balázs 16 Continue Dr. Visegrády Balázs


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17 The independent samples t test. The F test. Dr. Visegrády Balázs 18 Continue Dr. Visegrády Balázs 19 Linear regression and correlation. Dr. Visegrády Balázs 20 Continue Dr. Visegrády Balázs 21 Contingency tables 1. The chi-square test. Dr. Visegrády Balázs 22 Continue Dr. Visegrády Balázs 23 The non-parametric tests (sign test, Wilcoxon and Mann-Whitney tests). Dr. Visegrády Balázs 24 Continue Dr. Visegrády Balázs 25 The principle of the ANOVA. Summary of the hypothesis testing methods. Dr. Visegrády Balázs 26 Continue Dr. Visegrády Balázs 27 Medical tests. Sensitivity and specificity. (Contingency tables 2.) Summary. Dr. Visegrády Balázs 28 Continue Dr. Visegrády Balázs

Practices 1 Probability examples 1. + Using computers, Windows, 2 Continue 3 Probability examples 2 - discrete distributions. 4 Continue 5 The binomial distribution. 6 Continue 7 Exploring data by graphs. Continuous variables. Histogram. 8 Continue 9 Exploring data by numbers - descriptive statistics 10 Continue 11 Normal distribution. The distribution of the sample mean. 12 Continue 13 Estimations. The confidence interval of the expected value. 14 Continue 15 The hypothesis testing - the ‘five steps’. The one sample (and the paired samples) t tests. The sign test. 16 Continue 17 Estimation and hypothesis testing. The Type one and Type two errors. 18 Continue 19 The independent samples t test. 20 Continue 21 The linear regression and correlation. 22 Continue 23 Contingency tables - the chi-square test. 24 Continue 25 Nonparametric tests (Sign test, Wilcoxon tests, Mann-Whitney test) 26 Continue 27 Summary - Complex problems - prepare for the exam. 28 Continue

Seminars


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Exam topics/questions 1. The main goal of biometrics/biostatistics 2. The key feature of the statistical thinking - the probability. 3. The idea of the probability distribution - discrete distribution, binomial distribution 4. The idea of the probability distribution - discrete distributions, Poisson distribution 5. The basic principles of statistical thinking - from the data to the decision 6. Types of the data (variables) and displaying them with graphs 7. The population and the sample 8. Numerical description of continuous data 9. The idea of the probability distribution - continuous distributions 10. The normal distribution 1 11. The normal distribution 2 12. Statistical inference 13. The confidence interval of the population mean 14. The basic idea of hypothesis testing 15. The one sample and the paired t test 16. The confidence interval and the hypothesis testing 17. The risk of errors and the power of a test 18. The two (independent) samples t test 19. Connection between two variables - continuous variables 1 20. Connection between two variables - categorical variables 2 21. Evaluation of frequency data - 1. 22. Evaluation of frequency data - 2. 23. Nonparametric tests - 1. 24. Nonparametric tests - 2. 25. The principle of the ANOVA

Participants Dr. Bódis Emőke (BOEAAD.T.JPTE), Dr. Visegrády Balázs (VIBAAB.T.JPTE)


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OPABY1 BIOCHEMISTRY 1

Course director: DR. ZOLTÁN BERENTE, associate professor Department of Biochemistry and Medical Chemistry




Topic The subject covers the principles of chemical/biochemical processes occurring in living organisms. The 1st semester of the 2-semester Biochemistry course deals with the fundamental metabolic processes and with the function, structure and regulation of the enzymes, transporters and other proteins which participate in the above mentioned processes. The subject reveals the physical, chemical, thermodynamical and reaction-kinetical laws and rules of the essential metabolic processes and describes the structural characteristics of the participating small molecules. The subject lies down the foundations of the subjects Biochemistry 2, Pharmacology and Clinical Chemistry.

Conditions for acceptance of the semester It is mandatory to attend the lectures and laboratory practices. Requirements of the acceptance of Biochemistry 1 course are the following: - No more than three absences from the laboratory practices - Submission of at least 10 out of 12 short tests held in the first 10 minutes of the practices - Achieving satisfactory level in at least seven of the 12 short tests


Permission should be asked from the course director to do the practice with an other group. Permission will be given maximum 2 times.

Reading material Nelson - Cox: Lehninger Principles of Biochemistry, 5th Ed, W.H. Freeman, 2008. Berg - Tymoczko - Stryer: Biochemistry, 6th Ed, W.H. Freeman, 2007. Devlin: Textbook of Biochemistry with Clinical Correlations, 6th Ed, Wiley-Liss, 2006 Experimental Biochemistry, Edited by Dénes Szabó, 1990

Lectures 1 Structure and function of proteins, peptides Dr. Sümegi Balázs 2 Folding, the role of chaperons Dr. Berente Zoltán 3 Energetics Dr. Berente Zoltán 4 Basics of enzymatic catalysis Farkas Viktória 5 Regulation of enzymes, isoenzymes Dr. Kovács Krisztina 6 Hemoglobin, oxygen transport Dr. Kovács Krisztina 7 Proteins of the immune system Dr. Kovács Krisztina 8 Glycolysis I. Farkas Viktória 9 Glycolysis II. Farkas Viktória 10 Metabolic pathways coupled to the glycolysis Veres Balázs 11 Characterization of lipids, biological membranes Veres Balázs 12 Fatty acid oxidation Veres Balázs 13 The pyruvate dehydrogenase complex (PDC) Dr. Berente Zoltán 14 Reactions of the citric acid cycle Dr. Sümegi Balázs


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15 Regulation of the citric acid cycle Dr. Sümegi Balázs 16 Respiratory chain and its inhibition Dr. Sümegi Balázs 17 ATP synthesis ifj. Dr. Gallyas Ferenc 18 Mitochondrial transport processes, shuttles Veres Balázs 19 Supramolecular organization of functionally related enzymes Dr. Sümegi Balázs 20 Reactive oxygen species (ROS) Dr. Sümegi Balázs 21 Photosynthesis, light reaction Veres Balázs 22 Gluconeogenesis in plant cells from CO2 (dark reaction) and from acetate Veres Balázs 23 Gluconeogenesis in animal cells ifj. Dr. Gallyas Ferenc 24 Pentose phosphate cycle Farkas Viktória 25 Monosaccharides, disaccharides, UDP mechanisms Veres Balázs 26 Glycogen synthesis and degradation Dr. Kovács Krisztina 27 Concerted regulation of glycogen synthesis and degradation Dr. Kovács Krisztina 28 Fatty acid (palmitic acid) synthesis ifj. Dr. Gallyas Ferenc 29 Elongation and desaturation of fatty acids, formation of biologically active molecules ifj. Dr. Gallyas Ferenc 30 Synthesis of complex lipids ifj. Dr. Gallyas Ferenc 31 Synthesis of cholesterol and ketone bodies ifj. Dr. Gallyas Ferenc 32 Regulation and disorders of lipid metabolism Dr. Kovács Krisztina 33 Regulation and disorders of carbohydrate metabolism ifj. Dr. Gallyas Ferenc 34 Amino acid metabolism; the fate of the amino group Debreceni Balázs 35 Urea cycle Debreceni Balázs 36 Amino acid metabolism; the carbon skeleton Debreceni Balázs 37 Synthesis of non-essential amino acids Debreceni Balázs 38 Enzymopathies of amino acid metabolism Debreceni Balázs 39 Synthesis of biologically active molecules from amino acids Debreceni Balázs 40 Synthesis of purine and pyrimidine bases and nucleotides Farkas Viktória 41 Degradation of purine and pyrimidine bases and nucleotides Farkas Viktória 42 Overview of metabolism Dr. Sümegi Balázs

Practices


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Seminars 1 Preparation of buffer solutions 3 Properties of the proteins 5 Determination of protein concentration (Biuret method) 7 Basic biochemical laboratory techniques 9 Exploration of the contents of a cell, subcellular fractionation 11 Cell cultures 13 Polyacrylamide gel electrophoresis, Western Blot 15 Heart perfusion 17 SH-Enzymes, Warburg’s optical test 19 Study of the mitochondrial respiration (Clark electrode) 21 Enzyme kinetics, study of enzymatic inhibition 23 Noninvasive biochemical analysis 25 Affinity chromatography, study of Complex I 27 Consultation

Exam topics/questions The list of topics of the exam questions are identical to the list of the lectures. The exams, including the retaken exams are written exams of 30 open questions. Failure to answer more than two of the first 7

questions will automatically result in „failed” grade.

Participants Dr. Veres Balázs (VEBAAB.T.JPTE)


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OPABY2 BIOCHEMISTRY 2

Course director: DR. ZOLTÁN BERENTE, associate professor Department of Biochemistry and Medical Chemistry



Prerequisites: OPABY1 completed

Topic The subject serves as a foundation for the preclinical subjects Pathobiochemistry, Pharmacology, Pathophysiology, and the clinical subjects Internal Medicine, Clinical Chemistry and Neurology. The 2nd semester of the 2-semester Biochemistry course - based on the material covered by the 1st semester - gives a deeper view into the fundamentals of structure-function relationships of small- and macromolecules, especially focusing on the structure, function and regulation of the molecules that are involved in the storage and transmission of genetic information, and dealing with basic molecular biology techniques. Furthermore, the regulation mechanisms of metabolic, hormonal and signal transduction pathways on the levels of the cell as well as of the organism are covered. The special biochemical characteristics of the different organs are also discussed.

Conditions for acceptance of the semester It is mandatory to attend the lectures and laboratory practices. Requirements of the acceptance of Biochemistry 2 course are the following: - No more than three absences from the laboratory practices - Submission of at least 10 out of 12 short tests held at the first 10 minutes of the practices - Achieving satisfactory level in at least seven of the 12 short tests


Permission should be asked from the course director to do the practice with an other group. Permission will be given maximum 2 times.

Reading material Nelson - Cox: Lehninger Principles of Biochemistry, 5th Ed, W.H. Freeman, 2008. Berg - Tymoczko - Stryer: Biochemistry, 6th Ed, W.H. Freeman, 2007. Devlin: Textbook of Biochemistry with Clinical Correlations, 6th Ed, Wiley-Liss, 2006 P. Gergely (Ed.): Introduction to Bioinorganic chemistry for medical students (Univ. Med. School of Debrecen,latest edition) Experimental Biochemistry Edited by Dénes Szabó, 1990

Lectures 1 Genes and chromosomes Dr. Sümegi Balázs 2 DNA replication Dr. Sümegi Balázs 3 DNA repair Dr. Sümegi Balázs 4 RNA metabolism I Veres Balázs 5 RNA metabolism II Veres Balázs 6 Retroviruses Dr. Sümegi Balázs 7 Protein synthesis I Dr. Berente Zoltán 8 Protein synthesis II Dr. Berente Zoltán 9 Posttranslational modifications of proteins Dr. Berente Zoltán 10 Protein targeting and transport Dr. Kovács Krisztina 11 Regulation of gene expression I Dr. Sümegi Balázs 12 Regulation of gene expression II Dr. Sümegi Balázs


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13 The mitochondrial protein synthesis and the disorders of the mitochondrial genome Veres Balázs 14 Recombinant DNA technologies Debreceni Balázs 15 The cell cycle and its regulation Farkas Viktória 16 Hormones I. Dr. Berente Zoltán 17 Hormones II. Debreceni Balázs 18 Hormones III, PPARs Dr. Sümegi Balázs 19 Cell signalling, the CO and NO as signals, transcription factors Veres Balázs 20 Cell signalling induced by oxidative stress Dr. Sümegi Balázs 21 Biochemistry of septic shock Veres Balázs 22 Oncogenes and oncogenesis Debreceni Balázs 23 Tumor supressors and cancer Debreceni Balázs 24 Structure of the extracellular matrix Farkas Viktória 25 Cell signalling, kinases ifj. Dr. Gallyas Ferenc 26 Genetic disorders of hemoglobin Dr. Kovács Krisztina 27 Regulation of Fe metabolism, related diseases Dr. Kovács Krisztina 28 Biochemistry of digestion: macronutrrients Farkas Viktória 29 Biochemistry of digestion: micronutrrients Dr. Kovács Krisztina 30 Special functions of liver Dr. Kovács Krisztina 31 Serum lipoproteins Debreceni Balázs 32 Changes accompanying the switch from fed to fasting state ifj. Dr. Gallyas Ferenc 33 Biochemistry of the immune system ifj. Dr. Gallyas Ferenc 34 Water soluble vitamins Farkas Viktória 35 Lipid soluble vitamins Farkas Viktória 36 Biochemistry of the senses ifj. Dr. Gallyas Ferenc 37 Nervous system I. ifj. Dr. Gallyas Ferenc 38 Nervous system II. ifj. Dr. Gallyas Ferenc 39 Molecular targets of drug-development ifj. Dr. Gallyas Ferenc 40 Genomics, proteomics, metabolomics Dr. Berente Zoltán 41 Current chances for gene therapy and for tumor therapy Dr. Berente Zoltán 42 Current theories about the origin of life and evolution Dr. Berente Zoltán


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Practices

Seminars 1 The inorganic phosphate requirement of the glycolysis 2 MPT in apoptosis and necrosis 3 Study of body fluids 4 Molecular biology methods 5 Determination of blood glucose 6 Advanced biotechnology 7 Determination of blood cholesterol 8 Genomics, proteomics, bioinformatics 9 Determination of uric acid in blood 10 Hereditary metabolic diseases 11 Determination of bilirubin in blood 12 Acquired metabolic diseases 13 Study of cholinesterase 14 Consultation

Exam topics/questions The list of exam topics is identical to the list of the titles of lectures of both semesters. The exams, including the retaken exams are written exams of 40 open questions. Failure to answer more than three of the first 10

questions will automatically result in „failed” grade.

Participants Dr. Veres Balázs (VEBAAB.T.JPTE)


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OPAFI1 PHYSICS-BIOPHYSICS 1

Course director: DR. GÁBOR HILD , associate professor Department of Biophysics



Prerequisites: -

Topic The course addresses the physical basis of the structure and function of biological systems. The main topics include atomic and nuclear physics, thermodynamics, transport processes, molecular and supramolecular systems, bioelectric phenomena, and biological motion.

Conditions for acceptance of the semester Completion and proper documentation of each laboratory practice and approval thereof by the course instructor. Maximum 3 absences from practices. Students are not allowed to be late from the practicals. Being late counts as an absence.

Making up for missed classes Missed practices can be made up during make-up opportunities provided by the department. During each make-up lab, only one missed practice can be executed.

Reading material 1. Medical Biophysics (ed. Damjanovich Sándor, Fidy Judit, Szöllősi János). Medicina, Budapest, 2008 2. Biophysics Laboratory Manual, Pécs University Press, Pécs 3. Online materials on departmental website (http://biofizika.aok.pte.hu)

Lectures 1 Introduction to Biophysics Dr. Nyitrai Miklós 2 Diffusion Dr. Bugyi Beáta 3 Osmosis Dr. Bugyi Beáta 4 Fluid flow Dr. Hild Gábor 5 Biophysics of circulation. Cardiac biophysics Dr. Hild Gábor 6 Protein structure and folding Dr. Grama László 7 The cell membrane. Resting potential Dr. Hild Gábor 8 Sensory receptors, action potential Dr. Hild Gábor 9 Hearing Dr. Bódis Emőke 10 Vision Dr. Bódis Emőke 11 Cytoskeleton Dr. Bugyi Beáta 12 Motor proteins, cell motility Dr. Bugyi Beáta 13 Structure and mechanics of striated muscle Dr. Nyitrai Miklós 14 Molecular basis of muscle function and contraction regulation Dr. Nyitrai Miklós 15 Foundations of thermodynamics Dr. Visegrády Balázs


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16 Laws of thermodynamics Dr. Visegrády Balázs 17 Thermodynamic potentials Dr. Visegrády Balázs 18 Electromagnetic radiation, Electromagnetic spectrum Dr. Grama László 19 Foundations of quantum physics Dr. Grama László 20 Structure of the atom Dr. Grama László 21 Quantum numbers. Spin Dr. Grama László 22 Molecular orbitals. Singulet and triplet state Dr. Lukács András Szilárd 23 Laser Dr. Lukács András Szilárd 24 X-ray Dr. Talián Csaba Gábor 25 X-ray diffraction Dr. Talián Csaba Gábor 26 Structure of the atomic nucleus. Radioativity Dr. Orbán József 27 Interaction of radioactive radiation with matter Dr. Orbán József 28 Biological effects of radioactive radiation Dr. Orbán József

Practices 1 Introduction. Laboratory safety rules 2 Introduction. Laboratory safety rules 3 Direct current measurements 4 Direct current measurements 5 Alternative current measurements 6 Alternative current measurements 8 Electric conductivity. Refractometry 9 Electric conductivity. Refractometry 10 Spectroscopy and spectrophotometry 11 Spectroscopy and spectrophotometry 12 Polarimetry 13 Polarimetry 14 Make-up lab, seminar 15 Make-up lab, seminar 16 Viscosity of fluids 17 Viscosity of fluids 18 Surface tension 19 Surface tension 20 Adsorption and swelling 21 Adsorption and swelling 22 Centrifugation 23 Centrifugation 24 Electrophoresis 25 Electrophoresis 26 Make-up lab, seminar 27 Make-up lab, seminar 27 Make-up lab, seminar 28 Make-up lab, seminar

Seminars

Exam topics/questions Can be found on the departmental website: http://biofizika.aok.pte.hu


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Participants Dr. Bódis Emőke (BOEAAD.T.JPTE), Dr. Bugyi Beáta (BUBEAB.T.JPTE), Dr. Grama László (GRLHAAO.PTE), Dr. Hild Gábor (HIGMAAO.PTE), Dr. Kengyel András Miklós (KEAFACO.PTE), Dr. Lukács András (LUAHAAO.PTE), Dr. Visegrády Balázs (VIBAAB.T.JPTE), Kardos Roland (KARFAB.T.JPTE), Kollár Veronika Tünde (KOVGACT.PTE), Raics Katalin (RAKHAAT.PTE), Szatmári Dávid Zoltán (SZDHAAT.PTE), Tóth Mónika Ágnes (TOMIAAT.PTE), Türmer Katalin (TUKIAAT.PTE), Ujfalusi Zoltán (UJZDAA.T.JPTE), Vig Andrea Teréz (VIAFAAO.PTE)


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OPAFI2 PHYSICS-BIOPHYSICS 2

Course director: DR. GÁBOR HILD , associate professor Department of Biophysics



Prerequisites: OPAFI1 completed

Topic The course, stemming from „Physics-Biophysics 1” addresses the foundations of physical and biophysical methods used for exploring biological systems particularly the human body, as well as those of physical diagnostic methods. The latter are discussed briefly with references made to a respective topical pre-clinical course.

Conditions for acceptance of the semester Completion and proper documentation of each laboratory practice and approval thereof by the course instructor. Maximum 3 absences from practices. Students are not allowed to be late from the practicals. Being late counts as an absence.

Making up for missed classes Missed practices can be made up during make-up opportunities provided by the department. During each make-up lab, only one missed practice can be made up.

Reading material 1. Medical Biophysics (ed. Damjanovich Sándor, Fidy Judit, Szöllősi János). Medicina, Budapest, 2008 2. Biophysics Laboratory Manual, Pécs University Press, Pécs 3. Online materials on departmental website (http://biofizika.aok.pte.hu)

Lectures 1 Absorption photometry Dr. Orbán József 2 Absorption of proteins Dr. Orbán József 3 Infrared and Raman spectroscopy Dr. Orbán József 4 CD spectroscopy Dr. Orbán József 5 Luminescence basics Dr. Lukács András Szilárd 6 Measuring fluorescence and phosphorescence Dr. Lukács András Szilárd 7 Fluorescence polarization and anisotropy Dr. Lukács András Szilárd 8 FRET Dr. Lukács András Szilárd 9 Light microscopy Dr. Bugyi Beáta 10 Fluorescence microscopy Dr. Bugyi Beáta 11 Modern microscopic methods 1 Dr. Bugyi Beáta 12 Modern microscopic methods 2. FRAP Dr. Bugyi Beáta 13 Flow cytometry Dr. Grama László 14 Transient kinetics methods Dr. Nyitrai Miklós 15 NMR Dr. Visegrády Balázs 16 ESR Dr. Visegrády Balázs


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17 MRI 1 Dr. Visegrády Balázs 18 MRI 2 Dr. Visegrády Balázs 19 X-ray diagnostics Dr. Hild Gábor 20 CT Dr. Hild Gábor 21 Gamma-camera. SPECT Dr. Hild Gábor 22 PET Dr. Hild Gábor 23 Ultrasound diagnostics Dr. Grama László 24 Calorimetry (DSC, ITC) Dr. Bugyi Beáta 25 Sedimentation Dr. Talián Csaba Gábor 26 Electrophoresis Dr. Talián Csaba Gábor 27 Basics of Mass spectrometry Dr. Talián Csaba Gábor 28 Application of mass spectrometry Dr. Talián Csaba Gábor

Practices 1 Introduction. Laboratory safety rules 2 Introduction. Laboratory safety rules 3 The Geiger-Müller counter. Radioactive half-life I 4 The Geiger-Müller counter. Radioactive half-life I 5 Gamma-absorption and spectrometry 6 Gamma-absorption and spectrometry 7 Absorption of beta-radiation, dead time. Radioactive half-life II 8 Absorption of beta-radiation, dead time. Radioactive half-life II 9 Scintigraphy 10 Scintigraphy 11 Optics. Illumination 12 Optics. Illumination 13 Make-up lab, seminar 14 Make-up lab, seminar 15 Absorption photometry 16 Absorption photometry 17 Blood pressure. Electrocardiography 18 Blood pressure. Electrocardiography 19 Ultrasound 20 Ultrasound 21 Temperature measurement 22 Temperature measurement 23 Audiometry 24 Audiometry 25 Make-up lab, seminar 26 Make-up lab, seminar 27 Make-up lab, seminar 28 Make-up lab, seminar

Seminars

Exam topics/questions Can be found on the departmental website (http://biofizika.aok.pte.hu)

Participants Dr. Lukács András (LUAHAAO.PTE), Türmer Katalin (TUKIAAT.PTE)


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OPAFZ1 PHYSICAL CHEMISTRY 1

Course director: DR. GÉZA NAGY , professor Faculty of Natural Sciences - Department of General and Physical Chemistry



Prerequisites: OPABM1 completed

Topic The physical chemistry deals with the basic properties and structure of the matter. It discusses the chemical properties, events, reactions and interactions generally, using the tools of physics. This basic course on physical chemistry helps the students to understand the basic principles of chemistry and to handle quantitatively the chemical problems. The course intends to acquaint the students with the ways of calculating some basic physical chemical properties, changes, parameters needed for successful experimental work. The following main chapters are to be discussed: The laws of thermodynamics, and state functions The thermo chemistry and experimental techniques The chemical equilibrium Properties of gases, liquids and solids, Phase transitions Transport processes, kinetic theory of gases, Rate distribution law Reaction kinetics Electrochemistry The structure of the matter, atoms, chemical bonding Physical chemical basis of some instrumental methods

Conditions for acceptance of the semester The exam starts with a short written test. The students solve simple physical chemistry problems and answer basic questions about definitions, equations. Usually 20 questions are given. If the 10 of these are answered correctly than the student gets two randomly selected questions from the text covered in the lectures. After a short preparation time the oral section starts. The student standing in front of a black board, using chalk presents his answers. Score from 1 to 5 can be obtained. In case of failing in the written test or obtaining 1; the entire exam has to be repeated.

Making up for missed classes Being absent from three lectures will be tolerated. Text book and electronic hand out matter help students to catch up.

Reading material 1. P.W. Atkins and J. de Paula, Physical Chemistry, 8th edition, Oxford University Press (2002) 2. R. A. Alberty and R.J. Silbey, Physical Chemistry, 4th edition, John Wiley (2002) 3. K. J. Laidler, J. H. Meiser and B. C. Santuary, Physical Chemistry, Houghton Mifflin Company (2003)

Lectures

1 The scope of physical chemistry, The gas state, perfect and real gases, transport processes in gases, diffusion heat conduction, and viscosity, Heat capacity and structure in gases. The principle of corresponding states

Dr. Nagy Géza 2 Work heat and energy, basic concepts in thermodynamics, expansion work, internal energy, The first law, reversible and

irreversible expansion Dr. Nagy Géza 3 Enthalpy, Calorimetry, Thermo chemistry, Hess’s law, Kirchhoff’s law, Adiabatic changes Dr. Nagy Géza 4 TG, TA, DSC methods for investigation different processes, formation enthalpy Dr. Nagy Géza 5 Heat capacity, Joule-Thomson effect, changes in internal energy Dr. Nagy Géza 6 The Second law, entropy, changing of entropy in different processes, heat pumps, the Carnot cycle Dr. Nagy Géza 7 The third law of thermodynamics, The Helmholz and Gibbs energies, combination of the first and second Laws, the

fundamental equation Dr. Nagy Géza 8 Phase diagrams, stability of phases, phase transition, Clausius Clapeyron equation, supercritical fluids Dr. Nagy Géza 9 Dependence of phase stability on temperature and pressure Dr. Nagy Géza


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10 Pressure over curved surfaces, the surface tension, the Laplace equation, the Kelvin equation, capillary action Dr. Nagy Géza 11 Partial molar quantities, the thermodynamics of mixing, chemical potential of liquids, Gibbs Duham equation, Raoult’s

law, Henry’s law. Properties of liquid mixtures Dr. Nagy Géza 12 Colligative properties, lowering of freezing point, elevation of boiling point, osmosis Dr. Nagy Géza 13 Distillation, vapor pressure diagrams, the phase rule, liquid-liquid phase diagrams, liquid solid phase diagrams Dr. Nagy Géza 14 Chemical equilibrium, effect of pressure and temperature on equilibrium Dr. Nagy Géza 15 The kinetic model of gases, collision with walls and surfaces, effusion Dr. Nagy Géza 16 The rates of chemical reactions, Basic equations in case of elementary processes. Ways of studying reaction rate,

Determination of ages by reaction kinetic base Dr. Nagy Géza 17 Rate of complex reactions, consecutive reactions, parallel reactions, reactions approaching equilibrium Dr. Nagy Géza 18 Lindemann - Hinshelwood mechanism, steady state, rate laws of chain reaction, explosions, Dr. Nagy Géza 19 Enzyme catalysis, autocatalysis, oscillating reactions Dr. Nagy Géza 20 Heterogeneous reactions, photochemical reactions, polymerization Dr. Nagy Géza 21 The rate coefficient, collision theory, diffusion controlled reactions, Arrhenius equation Dr. Nagy Géza 22 Electrolytes, Debye-Hückel theory, mean activity coefficient, ionic strength. Formation enthalpy of ions. Dr. Nagy Géza 23 Conductivity in electrolytes, transfer number, conductivity measurements, its applications, Kohlrausch’s law, Ostwald’s

dilution law Dr. Nagy Géza 24 Electrode potential, electrodes, Nernst equation, Galvan cells, potentiometric cells, ion selective electrodes, the diffusion

potential Dr. Nagy Géza 25 Electrode processes, polarization, over potential, Tafel equation Dr. Nagy Géza 26 Polarization curves, Cottrell experiment, Faraday’s laws, electrolysis, Voltammetry, CV, corrosion Dr. Nagy Géza 27 Physical chemistry basis of instrumental methods, Spectroscopy Dr. Nagy Géza 28 Physical chemistry basis of instrumental methods, magnetic, electrical properties. Dr. Nagy Géza

Practices

Seminars

Exam topics/questions - The gas state, perfect and real gases, transport processes in gases, diffusion heat conduction, and viscosity, Heat

capacity and structure in gases. The principle of corresponding states - Work heat and energy, basic concepts in thermodynamics, expansion work , internal energy, The first law, reversible

and irreversible expansion - Enthalpy, Calorimetry, Thermo chemistry, Hess law, Kirchhoff law, Adiabatic changes - TG, TA, DSC methods for investigation different processes, formation enthalpy - Heat capacity, Joule-Thomson effect, changes in internal energy - The Second law, entropy, changing of entropy in different processes, heat pumps, th ecarnot Cicle - The third law of thermodynamics - The Helmholz and Gibbs energies, combination of the first and second Laws, the fundamental equation - Phase diagrams, stability of phases, phase transition, Clausius Clapeyron equation, supercritical fluids, - Dependence of phase stability on temperature and pressure, - Pressure over curved surfaces, the surface tension, the Laplace equation, the Kelvin equation, capillary action - Partial molar quantities, the thermodynamics of mixing, chemical potential of liquids, Gibbs Duham equation, Raoult’s

law, Henry’s law. Properties of liquid mixtures


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- Colligative properties, lowering of freezing point, elevation of boiling point, osmosis - Distillation, vapor pressure diagrams, the phase rule, liquid-liquid phase diagrams, liquid solid phase diagrams - Chemical equilibrium, effect of pressure and temperature on equilibrium, the description of equilibrium. - The kinetic model of gases, collision with walls and surfaces, effusion, - The rates of chemical reactions, Basic equations in case of elementary processes. Ways of studying reaction rate,

Determination of ages by reaction kinetic base - Rate of complex reactions, consecutive reactions, parallel reactions, reactions approaching equilibrium, - Lindemann - Hinshelwood mechanism, steady state, rate laws of chain reaction, explosions, - Enzyme catalysis, autocatalysis, oscillating reactions, - Heterogeneous reactions, photochemical reactions, polymerization - The rate coefficient, collision theory, diffusion controlled reactions, Arrhenius equation, - Electrolytes, Debye-Hückel theory, mean activity coefficient, ionic strength. Formation enthalpy of ions. - Conductivity in electrolytes, transfer number, conductivity measurements, its applications, Kohlrausch’s law, Ostwald’s

dilution law - Electrode potential, electrodes, Nernst equation - Galvan cells, potentiometric cells, ion selective electrodes, the diffusion potential - Electrode processes, polarization, over potential, Tafel equation - Polarization curves, Cottrell experiment, Faraday’s laws, electrolysis - Voltammetry, CV, corrosion

Participants


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OPAFZ2 PHYSICAL CHEMISTRY 2

Course director: DR. GÉZA NAGY , professor Faculty of Natural Sciences - Department of General and Physical Chemistry

3 credit ▪ midsemester grade ▪ Basic module ▪ autumn semester ▪ recommended semester: 3


Prerequisites: OPAFZ1 completed

Topic The students completing the course will get experience in carrying out basic physical chemical measurements. They will learn the ways of handling instruments, taking notes, evaluating experimentally taken data. Before the practice each student obtains an experimental task to be solved. In home reading they refresh the theoretical back ground and study the instructions. Before beginning the laboratory work they complete a short test to prove their level of understanding the theory and practice of the task to be solved. They work alone following the written instructions and guidelines of the instructor. The data, calculations, graphs, tables and conclusions are introduced into a carefully prepared note book. The tasks are taken from chapters of physical chemistry studied in course Physical chemistry I: Thermo dynamics, reaction kinetics, electro chemistry, equilibrium, properties of solutions, determination of indicator exponents. Different instruments like electrochemical work stations, spectro photometers, calorimeters, gas volume measurements, conductometric apparatus, pH meter and other basic “small” laboratory equipments are used.

Conditions for acceptance of the semester

Making up for missed classes One week absence can be tolerated if the experiment is performed in a supplementary time (last week)

Reading material Laboratory manual downloaded from the web site of the Dept for General and Physical Chemistry of Faculty of Sciences at the University of Pécs. P. Atkins, J. de Paula: Physical Chemistry 8th Edition, Oxford University Press 2006, ISBN 9780198700722 D. Freifelder: Physical Chemistry for Students of Biology and Chemistry, Science Books International Inc. ISBN 0 86720-002-2

Lectures

Practices 1 Determination of solubility, calculation of the differential dissolution heat 1 Determination of solubility, calculation of the differential dissolution heat 1 Determination of solubility, calculation of the differential dissolution heat 2 Investigation of the behavior of terner (three component) systems, preparation solubility triangle diagram 2 Investigation of the behavior of terner (three component) systems, preparation solubility triangle diagram 2 Investigation of the behavior of terner (three component) systems, preparation solubility triangle diagram 3 Investigation of the behavior Reaction heat measurement using adiabatic calorimeter 3 Investigation of the behavior Reaction heat measurement using adiabatic calorimeter 3 Investigation of the behavior Reaction heat measurement using adiabatic calorimeter 4 Investigation of kinetics of hydrolysis with conductometryc measurements 4 Investigation of kinetics of hydrolysis with conductometryc measurements 4 Investigation of kinetics of hydrolysis with conductometryc measurements 5 Investigation of decomposition kinetics of a drug molecule 5 Investigation of decomposition kinetics of a drug molecule 5 Investigation of decomposition kinetics of a drug molecule 6 Investigation of homogeneous catalysis (oxidation of ascorbic acid) 6 Investigation of homogeneous catalysis (oxidation of ascorbic acid) 6 Investigation of homogeneous catalysis (oxidation of ascorbic acid) 7 Study of drug dissolution kinetics from pharmaceutical pills using spectrophotometric method 7 Study of drug dissolution kinetics from pharmaceutical pills using spectrophotometric method 7 Study of drug dissolution kinetics from pharmaceutical pills using spectrophotometric method 8 Potentiometry, pH measurement, investigation of selectivity of ion selective electrodes, measurement of redox

potential. 8 Potentiometry, pH measurement, investigation of selectivity of ion selective electrodes, measurement of redox

potential. 8 Potentiometry, pH measurement, investigation of selectivity of ion selective electrodes, measurement of redox

potential. 9 Measurement of conductivity in electrolytes 9 Measurement of conductivity in electrolytes 9 Measurement of conductivity in electrolytes


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10 Investigation of kinetics of electrode processes, cyclic voltammetry, current - electrode potential curves, Cottrell form, Tafel’s equation



11 Determination of solubility products using potentiometric measurements 11 Determination of solubility products using potentiometric measurements 11 Determination of solubility products using potentiometric measurements 12 Determination of dissociation coefficient of color indicators using spectrophotometric method. 12 Determination of dissociation coefficient of color indicators using spectrophotometric method. 12 Determination of dissociation coefficient of color indicators using spectrophotometric method. 13 Computer modeling chemical reactions 13 Computer modeling chemical reactions 13 Computer modeling chemical reactions 14 Determination of diffusion coefficients 14 Determination of diffusion coefficients 14 Determination of diffusion coefficients

Seminars

Exam topics/questions

Participants Dr. Nagy Géza (NAGFAAP.PTE), Dr. Nagyné Zengő Lívia (NAZLAAF.PTE)


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OPAKD1 COLLOIDICS 1

Course director: DR. BARNA SZILÁRD KOVÁCS , associate professor Faculty of Natural Sciences - Department of General and Physical Chemistry



Prerequisites: OPAAT1 completed + OPAFZ1 completed

Topic Learning about the types, structure, stability, chemical and physical-chemical properties and about laws of interaction of colloidal systems.

Conditions for acceptance of the semester Two written tests should be completed (min. 50%) during the semester.


Reading material

Lectures 1 The colloidal state Dr. Kovács Barna Szilárd 2 Classification of colloidal systems Dr. Kovács Barna Szilárd 3 The structure of interfaces Dr. Kovács Barna Szilárd 4 Interfacial phenomena. Dr. Kovács Barna Szilárd 5 Surface tension. Dr. Kovács Barna Szilárd 6 Gas/liquid interface. Dr. Kovács Barna Szilárd 7 Interface of solution, phase boundary Dr. Kovács Barna Szilárd 8 Liquide/liquide interfaces Dr. Kovács Barna Szilárd 9 Solid/liquide interface Dr. Kovács Barna Szilárd 10 Capillary condensation. Wetting, wetting materials. Dr. Kovács Barna Szilárd 11 Adsorption phenomenon Dr. Kovács Barna Szilárd 12 Heat of adsorption, adsorption equations Dr. Kovács Barna Szilárd 13 Adsorption isotherms (Freundlich, Langmuir, Langmuir-Hückel, BET), Dr. Kovács Barna Szilárd 14 Adsorption hysteresis Dr. Kovács Barna Szilárd 15 Electric double-layer,electrokinetic potential Dr. Kovács Barna Szilárd 16 Electrokinetic phenomena Dr. Kovács Barna Szilárd 17 Properties of dispersed systems: distribution, morfology, degree of dispersity Dr. Kovács Barna Szilárd 18 Stability of colloidal systems, parameters that affect the stability Dr. Kovács Barna Szilárd 19 Aerosols, foams Dr. Kovács Barna Szilárd 20 Emulsions, suspensions Dr. Kovács Barna Szilárd


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21 Reology, properties and methodology. Dr. Kovács Barna Szilárd 22 Macromolecular colloids Dr. Kovács Barna Szilárd 23 Properties, examination methods of macromolecular colloids Dr. Kovács Barna Szilárd 24 Association colloids Dr. Kovács Barna Szilárd 25 Micelle formation Dr. Kovács Barna Szilárd 26 Parameters that affect the micelle formation Dr. Kovács Barna Szilárd 27 Liposomes Dr. Kovács Barna Szilárd 28 Langmuir Blodget films, LB technique. Dr. Kovács Barna Szilárd

Practices

Seminars

Exam topics/questions The colloidal state, classification of colloidal systems The structure of interfaces, interfacial phenomena. Surface tension. Gas/liquid interface. Interface of solution, phase boundary, liquide/liquide interfaces Solid/liquide interface, capillary condensation. Wetting, wetting materials. Adsorption phenomenon, heat of adsorption, adsorption equations, adsorption isotherms (Freundlich, Langmuir, Langmuir-Hückel, BET), adsorption hysteresis. Electric double-layer, electrokinetic potential, electrokinetic phenomena. Properties of dispersed systems: distribution, morfology, degree of dispersity. Stability of colloidal systems, parameters that affect the stability Aerosols, foams, emulsions, suspensions. Reology, properties and methodology. Macromolecular colloids, properties, examination methods. Association colloids, micelle formation, parameters that affect the micelle formation, liposomes, Langmuir Blodget films, LB technique.

Participants


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OPAMAN INSTRUMENTAL ANALYSIS

Course director: DR. FERENC KILÁR , professor Institute of Bioanalysis



Prerequisites: OPAAN2 completed

Topic The theory and practice of instrumental analysis.

Conditions for acceptance of the semester Successful written tests in the practices, oral examination, max two absences in the practices.

Making up for missed classes Maximum one absence can be recovered.

Reading material D.A.Skoog, F.J.Holler, S.R.Crouch: Principles of Instrumental Analysis, 6th Edition, Thomson, 2007

Lectures 1 Basis of forming instrumental signals Dr. Kilár Ferenc 2 Errors in instrumental analysis, signal collection Dr. Kilár Ferenc 3 Basic chemometry Dr. Kilár Ferenc 4 Electroanalysis (potentiometry, conductometry, voltammetry) Dr. Kilár Ferenc 5 Spectrophotometry Dr. Kilár Ferenc 6 Spectrophotometry Dr. Kilár Ferenc 7 Infrared spectroscopy, fluorescence spectroscopy Dr. Kilár Ferenc 8 Infrared spectroscopy, fluorescence spectroscopy Dr. Kilár Ferenc 9 Liquid chromatography, HPLC Dr. Kilár Ferenc 10 Liquid chromatography, HPLC Dr. Kilár Ferenc 11 Gas-chromatography Dr. Kilár Ferenc 12 Gas-chromatography Dr. Kilár Ferenc 13 Electrokinetic methods, electrophoresis Dr. Kilár Ferenc 14 Electrokinetic methods, electrophoresis Dr. Kilár Ferenc 15 Atomic absorption spectrometry Dr. Kilár Ferenc 16 Atomic absorption spectrometry Páger Csilla 17 Nuclear magnetic resonance spectrometry Dr. Kilár Ferenc 18 Nuclear magnetic resonance spectrometry Dr. Kilár Ferenc 19 Electron paramagnetic resonance spectrometry Dr. Kilár Ferenc 20 Electron paramagnetic resonance spectrometry Dr. Kilár Ferenc


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21 Mass spectrometry Dr. Kilár Ferenc 22 Mass spectrometry Dr. Dörnyei Ágnes 23 Analytical ultracentrifugation Dr. Kilár Ferenc 24 Analytical ultracentrifugation Dr. Kilár Ferenc 25 Mössbauer spectroscopy Dr. Kilár Ferenc 26 ORD, CD, polarimetry Dr. Kilár Ferenc 27 Thermal analysis Dr. Kilár Ferenc 28 Thermal analysis Dr. Kilár Ferenc

Practices 1 Potentiometry 4 Potentiometry 7 Conductometry 10 Spectrophotometry 13 Spectrophotometry 16 Atomic absorption spectrometry 19 Gas-chromatography 22 HPLC 25 Capillary electrophoresis 28 Chip-Capillary electrophoresis 31 EPR 34 NMR 37 Mass spectrometry 40 Recovery of missed practices

Seminars

Exam topics/questions http://aok.pte.hu/bioanalitika/e-index.php

Participants Borbásné dr. Farkas Kornélia (FAKAAA.T.JPTE), Dr. Bufa Anita (BUAEAA.T.JPTE), Dr. Dergez Tímea (DETCAAT.T.JPTE), Dr. Dörnyei Ágnes (DOASAAP.PTE), Dr. Kilár Ferenc (KIFGAAO.PTE), Dr. Poór Viktória (POVAAA.T.JPTE), Páger Csilla (PACMAAO.PTE)


38

OPASK2 ORGANIC CHEMISTRY 2

Course director: DR. KÁLMÁN HIDEG , professor emeritus Dept. of Organic and Pharmacological Chemistry



Prerequisites: OPASV1 completed

Topic This subject is a part of chemistry basics. Its aim to present the nomenclature, structure, physical and chemical properties, synthesis, reaction mechanisms and application of organic compounds.

Conditions for acceptance of the semester During the semester students have to accomplish the preparative works and written tests. They have to document the experiments in their exercise book.

Making up for missed classes Students have to contact their lab TA within 48 hours of missing lab to make the necessary arrangements.

Reading material John McMurry, Eric Simanek: Fundamentals of Organic Chemistry, 6th ed., Thomson Brooks, Belmont, 2007. T. W. Graham Solomons: Organic Chemistry, 7th edition, Wiley and Sons, New York, 2000. William H. Brown: Organic Chemistry, Saunders College Publishing, Fort Worth, 1995. Charles F. Wilcox, Jr., Mary F. Wilcox: Experimental organic chemistry: a small-scale approach, 2nd ed., Prentice-Hall, London, 1995. Dana W. Mayo, Ronald M. Pike, Peter K. Trumper: Microscale Organic Laboratory with Multistep and Multiscale Syntheses, 3rd ed., Wiley, New York, 1994.

Lectures 1 Aldehydes and ketones -structure, physical, chemical properties, reactions, important representatives. Dr. Hideg Kálmán 2 -II- Dr. Hideg Kálmán 3 -II- Dr. Hideg Kálmán 4 Carboxylic acids and their derivatives -structure, physical, chemical properties. Dr. Hideg Kálmán 5 -II- Dr. Hideg Kálmán 6 -II- Dr. Hideg Kálmán 7 alpha-Position substituted derivatives of carboxylic acids Dr. Hideg Kálmán 8 -II- Dr. Hideg Kálmán 9 -II- Dr. Hideg Kálmán 10 Di- and polycarboxylic acids, representatives Dr. Hideg Kálmán 11 -II- Dr. Hideg Kálmán 12 -II- Dr. Hideg Kálmán 13 Carbonic acid derivatives and their importance in biology and drugs Dr. Hideg Kálmán 14 -II- Dr. Hideg Kálmán 15 -II- Dr. Hideg Kálmán


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16 Esters of inorganic acids, biological significance (phosphatides, phospholipids). Dr. Hideg Kálmán 17 -II- Dr. Hideg Kálmán 18 -II- Dr. Hideg Kálmán 19 alpha-Amino acids, peptides and proteins, biological importance Dr. Hideg Kálmán 20 -II- Dr. Hideg Kálmán 21 -II- Dr. Hideg Kálmán 22 Monosaccharides -structure, mutarotation, reactions and biological importance Dr. Hideg Kálmán 23 -II- Dr. Hideg Kálmán 24 -II- Dr. Hideg Kálmán 25 Di- and polysaccharides -food reserve and structural material polysaccharides. Dr. Hideg Kálmán 26 -II- Dr. Hideg Kálmán 27 -II- Dr. Hideg Kálmán 28 Heterocycles (5- and 6-membered heteroaromatic compounds, structure, biologically important representatives,

chemical mode of action of coenzymes) Dr. Hideg Kálmán 29 -II- Dr. Hideg Kálmán 30 -II- Dr. Hideg Kálmán 31 -II- Dr. Hideg Kálmán 32 -II- Dr. Hideg Kálmán 33 -II- Dr. Hideg Kálmán 34 Heterocycles in nucleotides, nucleosides, nucleic acids Dr. Hideg Kálmán 35 -II- Dr. Hideg Kálmán 36 -II- Dr. Hideg Kálmán 37 Vitamins Dr. Hideg Kálmán 38 -II- Dr. Hideg Kálmán 39 -II- Dr. Hideg Kálmán 40 The basics of spectrometric identification of organic compounds Dr. Hideg Kálmán 41 -II- Dr. Hideg Kálmán 42 -II- Dr. Hideg Kálmán


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Practices 1 General instructions for work in the laboratory, safety precautions; Purification of benzaldehyde, synthesis of benzoin. 2 General instructions for work in the laboratory, safety precautions; Purification of benzaldehyde, synthesis of benzoin. 3 General instructions for work in the laboratory, safety precautions; Purification of benzaldehyde, synthesis of benzoin. 4 Synthesis of 2,6-dibenzylidene-cyclohexanone; Characteristic tube reactions of oxo compounds. 5 Synthesis of 2,6-dibenzylidene-cyclohexanone; Characteristic tube reactions of oxo compounds. 6 Synthesis of 2,6-dibenzylidene-cyclohexanone; Characteristic tube reactions of oxo compounds. 7 Synthesis of benzil; Identification of 4th unknown compound. 8 Synthesis of benzil; Identification of 4th unknown compound. 9 Synthesis of benzil; Identification of 4th unknown compound. 10 Characteristic tube reactions of carbohydrates; Preparation of oxalic acid, isolation of citric acid and pectin. 11 Characteristic tube reactions of carbohydrates; Preparation of oxalic acid, isolation of citric acid and pectin. 12 Characteristic tube reactions of carbohydrates; Preparation of oxalic acid, isolation of citric acid and pectin. 13 Acetylation of glucose; Identification of 5th unknown compound; 14 Acetylation of glucose; Identification of 5th unknown compound; 15 Acetylation of glucose; Identification of 5th unknown compound; 16 1st written test; Characteristic tube reactions of carboxylic acids; Synthesis of aspirin. 17 1st written test; Characteristic tube reactions of carboxylic acids; Synthesis of aspirin. 18 1st written test; Characteristic tube reactions of carboxylic acids; Synthesis of aspirin. 19 Synthesis of lidocain (1st step); Characteristic tube reactions of carboxylic acid derivatives 20 Synthesis of lidocain (1st step); Characteristic tube reactions of carboxylic acid derivatives 21 Synthesis of lidocain (1st step); Characteristic tube reactions of carboxylic acid derivatives 22 Synthesis of lidocain (2nd step) 23 Synthesis of lidocain (2nd step) 24 Synthesis of lidocain (2nd step) 25 Synthesis of heterocycles I.: benzimidazole, 4,5-diphenyl-imidazole; Characteristic tube reactions of heterocycles 26 Synthesis of heterocycles I.: benzimidazole, 4,5-diphenyl-imidazole; Characteristic tube reactions of heterocycles 27 Synthesis of heterocycles I.: benzimidazole, 4,5-diphenyl-imidazole; Characteristic tube reactions of heterocycles 28 Synthesis of heterocycles II.: dilantin, 4-methyl-7-hydroxycoumarine 29 Synthesis of heterocycles II.: dilantin, 4-methyl-7-hydroxycoumarine 30 Synthesis of heterocycles II.: dilantin, 4-methyl-7-hydroxycoumarine 31 2nd written test. Isolation of caffeine 32 2nd written test. Isolation of caffeine 33 2nd written test. Isolation of caffeine 34 Isolation of quercetin and myristic acid 35 Isolation of quercetin and myristic acid 36 Isolation of quercetin and myristic acid 37 Characteristic tube reactions of amino acids and peptides 38 Characteristic tube reactions of amino acids and peptides 39 Characteristic tube reactions of amino acids and peptides 40 Evaluation 41 Evaluation 42 Evaluation

Seminars

Exam topics/questions 1. Alkanes: Orbital hybridization and the structure of alkanes; Nomenclature, physical and chemical properties, conformation. 2. Alkyl, aryl halides: the character of C-X bonds, synthesis and reactions of alkyl halides (substitution, elimination); Organic

halides in the environment. 3. Unsaturated hydrocarbons: sp2 and sp hybridization, nomenclature, physical properties, reactions and synthesis of alkenes

and alkynes. 4. Importance of unsaturated hydrocarbons in industry and biology: polymerization, isoprene, terpenes, steroids, carotenoids. 5. Aromatic compounds: the structure of benzene, Hückel-rule. Electrophilic aromatic substitution reactions. 6. 5-membered heterocycles, heteroaromatic compounds, structure, biologically important representatives, chemical mode of

action of coenzymes). 7. 6-membered heterocycles, heteroaromatic compounds, structure, biologically important representatives, chemical mode of

action of coenzymes). 8. Alcohols, phenols, ethers: Structure, physical and chemical properties, reactions; Their biological importance. 9. Oxo-compondsnes – structure, physical, chemical properties, reactions, enol-oxo tautomerism, important

representatives.


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10. Carboxylic acids – structure, physical, chemical properties. 11. Alpha-Position substituted derivatives of carboxylic acids, di- and polycarboxylic acids, representatives. 12. Carboxylic acid derivatives – structure, physical, chemical properties. 13. Carbonic acid derivatives and their importance in biology and drugs. 14. Alpha-Amino acids, peptides and proteins, biological importance. 15. Esters of inorganic acids, biological significance (phosphatides, phospholipids). 16. Organometallic compounds: their importance in organic syntheses (Mg, Na, Li, Zn, Si, Cu, Cd compounds). 17. Sulfur containing compounds: Physical, chemical properties, importance in biological processes (in amino acids, peptides,

methylation in biology, AcCo-A, drugs). 18. Aliphatic and aromatic nitro, azo and diazo compounds: Structure, reactions, significance in organic syntheses. 19. Amines: nomenclature, physical and chemical properties, basicity, preparation. 20. Biologically active amines: Alkaloids, nitrogen containing drugs and hormones. 21. Monosaccharides: structure, mutarotation, reactions and biological importance. 22. Di- and polysaccharides: food reserve and structural material polysaccharides. 23. Heterocycles in nucleotides, nucleosides, nucleic acids. 24. Stereochemistry: isomerism, configuration, chirality.

Participants Dr. Kálai Tamás (KATFABP.PTE)


42

OPASV1 ORGANIC CHEMISTRY 1

Course director: DR. KÁLMÁN HIDEG , professor emeritus Dept. of Organic and Pharmacological Chemistry




Topic This subject is a part of chemistry basics. Its aim to present the nomenclature, structure, physical and chemical properties, synthesis, reaction mechanisms and application of organic compounds.

Conditions for acceptance of the semester During the semester students have to accomplish the preparative works and written tests. They have to document the experiments in their exercise book.

Making up for missed classes Students have to contact their lab TA within 48 hours of missing lab to make the necessary arrangements.

Reading material John McMurry, Eric Simanek: Fundamentals of Organic Chemistry, 6th ed., Thomson Brooks, Belmont, 2007. T. W. Graham Solomons: Organic Chemistry, 7th edition, Wiley and Sons, New York, 2000. William H. Brown: Organic Chemistry, Saunders College Publishing, Fort Worth, 1995. Charles F. Wilcox, Jr., Mary F. Wilcox: Experimental organic chemistry: a small-scale approach, 2nd ed., Prentice-Hall, London, 1995. Dana W. Mayo, Ronald M. Pike, Peter K. Trumper: Microscale Organic Laboratory with Multistep and Multiscale Syntheses, 3rd ed., Wiley, New York, 1994.

Lectures 1 The system of organic compounds, functional groups, reaction mechanisms. Dr. Hideg Kálmán 2 -II- Dr. Hideg Kálmán 3 -II- Dr. Hideg Kálmán 4 Alkanes -Orbital hybridization and the structure of alkanes; Nomenclature, physical and chemical properties,

conformation. Dr. Hideg Kálmán 5 -II- Dr. Hideg Kálmán 6 -II- Dr. Hideg Kálmán 7 Unsaturated hydrocarbons -sp2 and sp hybridization, nomenclature, physical properties, reactions and synthesis of

alkenes and alkynes. Dr. Hideg Kálmán 8 -II- Dr. Hideg Kálmán 9 -II- Dr. Hideg Kálmán 10 -II- Dr. Hideg Kálmán 11 -II- Dr. Hideg Kálmán 12 -II- Dr. Hideg Kálmán 13 Importance of unsaturated hydrocarbons in industry and biology -polymerization, isoprene, terpenes, steroids,

carotenoids. Dr. Hideg Kálmán 14 -II- Dr. Hideg Kálmán 15 -II- Dr. Hideg Kálmán


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16 Aromatic compounds -the structure of benzene, Hückel’s rule. Electrophilic aromatic substitution reactions. Dr. Hideg Kálmán 17 -II- Dr. Hideg Kálmán 18 -II- Dr. Hideg Kálmán 19 Alkyl halides -the character of C-X bonds, synthesis and reactions of alkyl halides (substitution, elimination); Organic

halides in the environment. Dr. Hideg Kálmán 20 -II- Dr. Hideg Kálmán 21 -II- Dr. Hideg Kálmán 22 Organometallic compounds -their importance in organic syntheses (Mg, Na, Li, Zn, Si, Cu, Cd compounds). Dr. Hideg Kálmán 23 -II- Dr. Hideg Kálmán 24 -II- Dr. Hideg Kálmán 25 Alcohols, phenols, ethers -Structure, physical and chemical properties, reactions; Their biological importance. Dr. Hideg Kálmán 26 -II- Dr. Hideg Kálmán 27 -II- Dr. Hideg Kálmán 28 Stereochemistry: isomerism, configuration, chirality. Dr. Hideg Kálmán 29 -II- Dr. Hideg Kálmán 30 -II- Dr. Hideg Kálmán 31 Sulfur containing compounds -Physical, chemical properties, importance in biological processes (in amino acids,

peptides, methylation in biology, AcCo-A, drugs). Dr. Hideg Kálmán 32 -II- Dr. Hideg Kálmán 33 -II- Dr. Hideg Kálmán 34 Aliphatic and aromatic nitro, azo and diazo compounds -Structure, reactions, significance in organic syntheses. Dr. Hideg Kálmán 35 -II- Dr. Hideg Kálmán 36 -II- Dr. Hideg Kálmán 37 Amines -nomenclature, physical and chemical properties, basicity, preparation. Dr. Hideg Kálmán 38 -II- Dr. Hideg Kálmán 39 -II- Dr. Hideg Kálmán 40 Biologically active amines -Alkaloids, nitrogen containing drugs and hormones. Dr. Hideg Kálmán 41 -II- Dr. Hideg Kálmán 42 -II- Dr. Hideg Kálmán


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Practices 1 General instructions for work in the laboratory, safety precautions. Introduction to use of common laboratory apparatus.

Characteristic reactions of alkanes, alkenes and alkynes. 2 General instructions for work in the laboratory, safety precautions. Introduction to use of common laboratory apparatus.

Characteristic reactions of alkanes, alkenes and alkynes. 3 General instructions for work in the laboratory, safety precautions. Introduction to use of common laboratory apparatus.

Characteristic reactions of alkanes, alkenes and alkynes. 4 Basic laboratory operations I.: Synthesis of acetanilide (heating, cooling, stirring, filtration with vacuum,

crystallisation, determination of the melting point). 5 Basic laboratory operations I.: Synthesis of acetanilide (heating, cooling, stirring, filtration with vacuum,

crystallisation, determination of the melting point). 6 Basic laboratory operations I.: Synthesis of acetanilide (heating, cooling, stirring, filtration with vacuum,

crystallisation, determination of the melting point). 7 Basic laboratory operations II.: Separation of dies with column chromatography; Identification of 1st unknown

compound. Characteristic tube reactions of aromatic hydrocarbons. 8 Basic laboratory operations II.: Separation of dies with column chromatography; Identification of 1st unknown

compound. Characteristic tube reactions of aromatic hydrocarbons. 9 Basic laboratory operations II.: Separation of dies with column chromatography; Identification of 1st unknown

compound. Characteristic tube reactions of aromatic hydrocarbons. 10 Basic laboratory operations III.: Synthesis of tert-butyl-chloride (extraction, drying, filtration, distillation, determination

of the boiling point); Characteristic tube reactions of alkyl halides. 11 Basic laboratory operations III.: Synthesis of tert-butyl-chloride (extraction, drying, filtration, distillation, determination

of the boiling point); Characteristic tube reactions of alkyl halides. 12 Basic laboratory operations III.: Synthesis of tert-butyl-chloride (extraction, drying, filtration, distillation, determination

of the boiling point); Characteristic tube reactions of alkyl halides. 13 1st written test; Synthesis of p-bromo-acetanilide and p-nitro-acetanilide. 14 1st written test; Synthesis of p-bromo-acetanilide and p-nitro-acetanilide. 15 1st written test; Synthesis of p-bromo-acetanilide and p-nitro-acetanilide. 16 Characteristic tube reactions of alcohols, phenols and ethers. 17 Characteristic tube reactions of alcohols, phenols and ethers. 18 Characteristic tube reactions of alcohols, phenols and ethers. 19 Synthesis of fluorenol with reduction and fluorenone with oxidation; Identification of 2nd unknown compound; 20 Synthesis of fluorenol with reduction and fluorenone with oxidation; Identification of 2nd unknown compound; 21 Synthesis of fluorenol with reduction and fluorenone with oxidation; Identification of 2nd unknown compound; 22 Basic laboratory operations IV.: Continuous extraction, thin layer chromatography, measurement of optical activity,

infrared spectroscopy. Isolation of piperine. 23 Basic laboratory operations IV.: Continuous extraction, thin layer chromatography, measurement of optical activity,

infrared spectroscopy. Isolation of piperine. 24 Basic laboratory operations IV.: Continuous extraction, thin layer chromatography, measurement of optical activity,

infrared spectroscopy. Isolation of piperine. 25 Preparation of 4-amino-benzenesulfonamide and nitrobenzene. 26 Preparation of 4-amino-benzenesulfonamide and nitrobenzene. 27 Preparation of 4-amino-benzenesulfonamide and nitrobenzene. 28 Preparation of 4-amino-benzenesulfonamide and nitrobenzene. 29 Preparation of 4-amino-benzenesulfonamide and nitrobenzene. 30 Preparation of 4-amino-benzenesulfonamide and nitrobenzene. 31 2nd written test. Characteristic reactions of amines. 32 2nd written test. Characteristic reactions of amines. 33 2nd written test. Characteristic reactions of amines. 34 Preparation of anthranilic acid; Identification of 3rd unknown compound. 35 Preparation of anthranilic acid; Identification of 3rd unknown compound. 36 Preparation of anthranilic acid; Identification of 3rd unknown compound. 37 Preparation of 2-iodobenzoic acid; 38 Preparation of 2-iodobenzoic acid; 39 Preparation of 2-iodobenzoic acid; 40 Evaluation. 41 Evaluation. 42 Evaluation.

Seminars


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Exam topics/questions 1., The system of organic compounds, functional groups, reaction mechanisms. 2., Alkanes: Orbital hybridization and the structure of alkanes; Nomenclature, physical and chemical properties, conformation. 3-4., Unsaturated hydrocarbons: sp2 and sp hybridization, nomenclature, physical properties, reactions and synthesis of alkenes

and alkynes. 5., Importance of unsaturated hydrocarbons in industry and biology: polymerization, isoprene, terpenes, steroids, carotenoids. 6., Aromatic compounds: the structure of benzene, Hückel-rule. Electrophilic aromatic substitution reactions. 7., Alkyl halides: the character of C-X bonds, synthesis and reactions of alkyl halides (substitution, elimination); Organic halides

in the environment. 8., Organometallic compounds: their importance in organic syntheses (Mg, Na, Li, Zn, Si, Cu, Cd compounds). 9., Alcohols, phenols, ethers: Structure, physical and chemical properties, reactions; Their biological importance. 10., Stereochemistry: isomerism, configuration, chirality. 11., Sulfur containing compounds: Physical, chemical properties, importance in biological processes (in amino acids, peptides,

methylation in biology, AcCo-A, drugs). 12., Aliphatic and aromatic nitro, azo and diazo compounds: Structure, reactions, significance in organic syntheses. 13., Amines: nomenclature, physical and chemical properties, basicity, preparation. 14., Biologically active amines: Alkaloids, nitrogen containing drugs and hormones.

Participants Dr. Kálai Tamás (KATFABP.PTE)

Documents

PHARMACY Major STUDY PROGRAM 2012/2013 Subjects of the …aok.pte.hu/.../tantervek_2012/course_eng_pha_basic_1213.pdf · 2012-08-13 · Medical School PHARMACY Major STUDY PROGRAM