Olympus Mg Instructions

8/12/2019 Olympus Mg Instructions

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lympus invertedmet llurgic l

~ \ ~ l r ~ ~ ~ [ ~ r ~ [ l ~ \ microscope


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Contents

I Standard setII Principal parts

Specifications 3 Optical path diagram 3V Structure 4VI How to assemble 6W How to use 7

A. Adjustment of the movement of the coarse adjustmenthandle

B. Place the specimen of the stageC. Lighting of the lampD. Centering of light source lamp and adjustment of

diaphragmsE. Focusing 9F. Dioptric adjustmentG. Adjustment of interocular distanceH. How to use immersion objective MIOOxI. Photo graphy 0J Observation with polarized light

\fl Characteristics of lenses IX Important points to remember


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Size 30 x 13Size 30 x 13

I tandard setMicroscope body

Main body focusing mechanism microscope, transformer)Binocular headRevolving nosepieceStageCamera mounting ringIlluminating apparatus illumination tube, lamphouse bulb socket)

ObjectivesM5x MIOx M40x MI00x

EyepiecesBinocular eyepieces P7 x WF 10 x P15 x

Viewfinder eyepiecesDK20x

35mm cameraStage insert plates

2 < > hole 10 x 30 hole Graduated 1O< > holeStage clips

Type IType n

PolarizerFilters

Light green G-533)Yellow Y-48)

Spare bulb6V 2A

Cable release slip-on type)Dust covers

1 set

1 each

1 pair each

1

1 each

1 set2 sets

11 each

lamp is 12W6 Filters fo r color photography3 color temperature conversionfi Iters20LB-45, 20LB-IOO, 20LB-200)3 NO fi lters20NO-3, 20NO-12, 20NO-50)Filter receiving rods7 Very low power objectivesM Plan 1 3X M Plan 2.5XFor use in microscopic observationanb photographyFor use in combination with theoblique light apparatus

For illumination tube mounting holecamera mounting hole camera eyepiece sleevs

Cargi lle oilVinyl coverInspection certificate Optional accessories

1 Dry plate photograph ic apparatusFor dry plate photography.2 Polaroid Land Camera The Polaroid camera uses a roll film.3 Mamiya roll film holderThis photographic unit uses a 120roll film and makes 8 negatives perroll.4 Transmitted light apparatusThis apparatus is used fo r observa-tion of a specimen with a transmittedlight. Lamp: 12W5 Oblique l ight apparatusThis is an apparatus to illuminate aspecimen obliquely. The light source

Polaroid, registered trade mark of Polaroid Corporation,

1 each

1 vial

Cambridge Mass U S A


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rincipal partsStage ns r t plate

Stage

I l luminator

Eyepiece

Binocular head

Main body

35mm camera

T l pecifications

Stage clips

Objective

Revo lv ing nosep iece

movementhandle

Stage vert icalmovement hand le

Analyser knob

Microscope body Binocular, inclinded 30 to the horizontal plane interocularadjustment : 5 5 5 ~ 7 4standard interocular distance 62 c l ick s top , d iopt ri cadjustment ring

Revolving nosepiece: Quadruple ball-bearing typeTotal magnif ication : 3 5 x ~ 1 5 0 0 xFocusing: Coaxial coarse and fine adjustment system by moving the stage up and downCoarse adjustment: Rack and pinion system, range of adjustment : 55mm.Fine adjustment: Lever and micrometer screw range of vertical movement 2mm,

minimun graduation; 0.005mm


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Optical path diagram

Objective

Prism

Optical path shifting prism for Bi head

Film plane

Illuminator lensloc ted 11 the right side s viewedfrom in front of the m in ody

PolarizerI techable t

Analyser

Shutter

hoto eyep iece

Reflecting Mirror.

Stage: Coaxial control knobs with low drive. Graduated mechanical stage with 24 x 24mmmovement, reading to 0.1mm by vernier

I l luminator: Vertical illuminating system with coated plane glass reflector, filter slot,polarizer and analyzer built-in , field and aperture diaphragms and6V-12W bulb

Transformer: Built in the main body, variable vol tage: 0 9VShutter with Speeds: B 1 1 /2 1 /5 1/10 1 /25 1 /50


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tructure Main body

hu tt r cocking lever

Shutter speed dial

amera f ix ing knoblLoosen this knob forr ep la ci ng camera whenphotographic unit otherthan bmm camera isusedamera mount ingring

amera

Grounding Wire terminal

icroscope head and Revolving nosepiece

Hole fo r mount ingobject ive

I nt erocu la r d is tancesc ale rin gThe s ca le indicates the interpupillarydistance of the eyes

hut t r release but tonCable release can bea ttached here

Pilot lampThis lamp is lighted whenthe power source is sw -itched on-- -

Main switch

Voltage controlhandleFor controlling thebrightness ofillumina tion

Base

Secondary socketFo r connecting thesecondary cord

Primary cordTo be connected to an electric outlet

Revolv ing nosep iece

Interocular adjustment rangeTh e binocular eyepieces can bemoved sideways click stoppedt th graduation of 6


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I l luminator f as te n in g s c re w

I l luminatorm ou nt in g h ole

Pr ism shi t in knTh e o pt ic al p at h g oe s to camerawhen th e knob is pulled ou t andturns to microscope head whenth e knob is pushed in.

ocusing mechanism and StageHole fo r re ce iv in g s ta ge i ns er t plate

S ta ge v er ni er scalesLateral movement scale is on frontand scale fo r back forthmovement is on left.Both scales have avernier that measuresto 0.1.Th e working distancefo r either direction is 4mm

B Illuminator

E ye p ie ce s le e ve

Dioptricadjustment r ingOnly the loft hand eyepiece tube ha sthis ring fo r dioptric adjustment.

Stage c li p m ount ing hole

Th e stage can ue moved24mm backward and Iorwnrd.

Lateral movemen t hand leTh e stage r-un be moved24mm side to side

Coarse adjustment scaleA r-ough udiustment of focuscan be obtalned by operating ,the COUI se adjustment handle

Fine adjustment handle fine ndjustmen L of focus cunbe made by this handleThe wor-king distance is 210m

Coarse adjustment handleU se d f or a rough adjustment of focus.Workint{ distance is 55mm,

Socket Lamphouse I ll uminat ion tuber \

Centering knobFo r centering lamp

Socket clamp knob

Secondary cordTo connect to th ese conda ry socke t

Lamphouse clamp knob

5

Posit ioni ng screwFor setting t he p os it io n ofth e illuminator assemblywhen it is attached to themicroscope body

Field diaphragm ringF markings ar e given used rchanging th e size of fielddiaphragm aper ture

C ov er g la ss ringWhen polarizer and analyser ar enot in use the window should becovered

perture diaphragm r ing


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C. CameraFilm winding knobFOI winding th e filmFilm counterI ndi ca t es t he number offrames exposed

Red do t )Match this to the reddot ( . )on the cameramounting ring.

VI. w t assembleA. Mount the illuminating apparatus.

1. Inser t the illuminator assembly into the holeon the left side of the microscope body in amanner in which the pos it ioning screw f i tin the pos it ioning groove Q)

2. Fasten the i l lumination tube fastening knob @to lock the l ight scurce unit in position.

B. Attach objectivesRaise the stage and mount four objectives in theholes in the nosepiece.It is advisable fo r the sake of convenience thatthe objectives are arranged iu the order of theirmagnifying powers.

C. Put the stage insert plateChoose one of the three stage insert plates tosuit the purpose of specimen observation and dropit into the hole in the center of the stage.

Fig. 3

Film wind l oc k r el ease buttonDepr-ess this bu tt on bef or e eachand every film winding action.

Fi lm rewinding knobFor r ew ind ing the fi lm.

Film wind ndjustment leverFo r photography se t t hi s l ev er

to A or D :Fo r film rewinding se t thisto R :

Bayonet mount

Rear cover lockThe camera back can be opened orclosed by opera ti n g thi s lock.

Fig. 1

ohe lOx30 st ge

insert p late with n . .oblong holeThe graduated stage insert plate


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D tt ch the clips on the stagePut the c lip shafts through the cl ips and screwthem on to the stage. When a graduated s tage inser t plate is used

the clips are installed on the s tage inser tplate.

ll ow to usdjustment of the movement ofthe coarse adjustment handle

The coarse adjustment handle is so designed thatit s movement is heavy and stiff. However it canbe adjusted according to the user s preference.If you grip the handles in both hands and turnthem in a reversed direction, the handles areloosened so t ha t they can be moved easily.The movement of the handles will become heavierif the handles are turned in the oppositedirection.

B Place the specimen on the stagePlace the specimen on the stage with its surfaceto be examined facing downward and lower thestage clips along the ir shafts and then thespecimen will be fixed on the stage.

C Lighting of the lamp1. Make sure that the voltage control handle is at

the left extreme of the scale and the powersource switch is set at OFF and then connectthe secondary cord to the secondary socketand the primary cord to the electr ic outlet .

2 Turn t he power source switch to ON.Turning the power switch on the light sourcelamp and the pilot lamp are lighted at the sametime.

3 Adjustment of brightnessAdjus t the brightness o f ligh t source byoperating the vol tage control handle.

D Centering of light source lampand adjustment of diaphragms

Use the objective MlO x. Insert eyepieces (anymagnification) in place and focus the specimenby moving the stage up and down by operatingthe stage vertical movement handle.(For furtherdetails, reference should be made to Par. E.Focusing )

Fig 4

Fig. 5

Fig. 6


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2. Turn the f ie ld diaphragm ring with F numbermarkings) toward Min to c lose down thediaphragm to the min imum aperture and youwill see a polyonal image of the diaphragm inthe center of the field.

3. Looking through the eyepiece, slowly turn thefield diaphragm r ing toward Max to open theaperture until the image of the diaphragmdisappears from viewfield. This process should be repeated every t ime theeyepieces are changed.

4. Remove the eyepieces. Looking through theeyepiece tubes, turn the aperture diaphragmring with A markings)toward Min to close thediaphragm down to the minimum aperture andthen open it until the image of the diaphragmdisappears from the viewfie ld.

Repeat this process every time the objec tiveis changed.5. Looking through the eyepiece tube, you willsee the image of the filament of the l ightsource. Operate the two centering knobs sothat the image comes into the center of thefield.

Fig. 9

Fig. 8

Fig. 7@ - 0 , ..-- ..

CD

Complete centeringncomplete centering6. Replace the eyepieces into the eyepiece tubes

and now the microscope is ready fo r use. If the f ie ld is unevenly illuminated, loosen thelamphouse clamping knob and adjust theposition of the lamp by shifting it backwardand forward.

@ Replacement of the lamp1. Loosen the socket fastening knob and pull ou t the socket.2. Hold the bulb in fingers and twist it to the left while applying a slight pressure on itand the bulb can be removed from the socket. Careful ly wipe tingerprtnts and stains from a new bulb before using it . If the f ie ld diaphragm and aperture diaphragm are no t correctly adjusted, it w ill result

in an unnecessary reflection of light or scattering light against the specimen. This willdeter iorate the resolving power of the objective and the contrast in the viewfie ld.When the lamp filament, the field diaphragm, and the aperture diaphragm are adjusted,it is essential that the surface of the specimen to be examined is at right angles to theoptical axis of the instrument. Before making such adjustments, you should always makesure that the above essential condition been set up.If the surface of the specimen to be examined has irregularities, it will make the aboveadjustments difficult. For this reason, i t is suggested that a well pol ished specimen isused.


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E Focusing1. Use the objective MI0X. Lower the s tage all the

way by operating the coarse adjustment handle.2. Looking through the eyepieces slowly raise the

stage by using the coarse adjustment handleto make a rough adjustment of focus.The coarse adjustment is so designed that arough adjustment of focus is obtained whenthe coarse adjustment marking lines on theright side have been matched.

3 Rotate the nosepiece to put a desired objectivein the place and make an accurate adjustmentof focus by turning the f ine adjustment handle.

F Dioptric adjustmentLook through the right hand eyepiece with righteye and f ocus the specimen. Next look throughthe left hand eyepiece with left eye and adjustthe focus by turning the dioptric adjustment ringNow the eyepieces are correctly adjusted fo r theeyes of the observer.

G djustment of interocular distanceAdjus t the binocular eyepiece tubes to theinterocular distance of the observer by slidingthe eyepiece tubes inward and outward.

H How use immersion objective OOXThe immersion objective MlOOX has twoidentification marks HI and a black line near thefront end of the lens tube.

Fig. 1

Fig. 11

Fig. 12

1. Focus the specimen with objective M lOX.2. Apply a drop of cargille oil to the front lens of immersion objective MI00X.3. Rotate the revolver to pu t the immersion objective in the place.4. When the oil on the front lens of the immersion objective has made contact with the

specimen focus the specimen by turning th e fine adjustment handle.5. After the immersion objective is used carefully wipe the oil from the lens using a piece of

soft gauze moistened with xylol. If the oil is left on the objective it will have an adverse effect on the lens.


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I j tf 1 H --Tt ~ ~

hotogr phy 5 J e L TJ \ = I/ ) ,B P1v\ 0

The c am er a takes 24mmX36mm pictures.Magnification on film plane:

Magnification of objective X magnificationof eyepiece l OX) X about 0. 7

1. Load th e film in th e camera1 Flip up the rear c ov er l oc k. After turning it

counterclockwise, slide th e camera backdownwards as shown in th e photograph.

2) Place th e film cartridge in the cartridgec ha mb er at th e left in th e camera. Make surethat t he p erfo rat io ns on both edges of th efilm have properly engaged with the sprocketand then replace th e rear cover by followingth e reversed procedure described in 1

3) Set th e film wind adjustment lever to A andturn th e film wind knob in the direction ofth e arrow. Push th e film wind lock releasebutton and wi nd th e film again.Turn th e film wind knob twice more toadvance tw o frames.

Match th e 0 mark on th e film counter towhitei :, mark on th e camera body. The dialindicates the number of exposed frames.)Turn th e film counter dial with a fingerg ri pp in g t he stud on the dial.

Fig. 155) Mount the camera . Match the r e d mark at

th e upper side of th e camera with the redmark on the camera mo un t in g r in g and turnth e c ame ra in the direction of t he a rr ow u nt ilit comes to a stop.

Remove the cameraTurn the camera counterclockwise while pres-

sing th e camera release knob. the camera willcome o ff when the red marks are matched.

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Fig, 13

rig. 14

Fig. 16

Fig. 17


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6 Attach the cable release.

2. Set the photographic eyepiece1 Adjust the interocular distance of theeyepieces at 62 click stopped , when the

focal plane of the binocular eyepieces and thefocal plane on the film come into perfectcoincidence.

2 Replace the right-hand eyepiece with K20Xviewfinder eyepiece and adjust i t by turningthe eyepiece ring so that the double linescome into focus.The frame which is viewed within the fieldshows the picture area.

3 Operate the fine adjustment handle to makean accurate adjustment of focus on thespecimen.

4 Pullout the prism shifting knob on the leftside so that the optical path may turn tothe camera.

3. Photographing procedures1 Choose a proper shutter speed and se t the

shutter speed dial.

Fig. 212 Cock the shutter by pushing the shutter

cocking lever.3 Release the shut ter slowly using the cable

release. Now you have exposed a fram.4 After exposing a frame, push the film wind

lock release button and advance the film fo rnext frame by turning the film wind knob.

5 For rewinding the film, set the film windadjustment lever at R, and erect the rewindlever and turn i t in the direction of the arrow.

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Fig. 18

Fig. 19

Fig. 20

Fig. 22

Fig. 23


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Observation with polarized lightObservation with polarized l ight is possible byusing the accessory polarizer and the built-inanalyser This method is useful fo r examiningcrystal structure and nonmetal substancespresent in a specimen.1 Turning the cover glass r ing, open to the ful l

the slot fo r insertion of the polarizer.2 Insert the polarizer into the slot.

In inserting the polarizer, the P mark etchedon t he frame of the polarizer should be facedtoward t he mark ings 0 , 45 90 on thei l lumination tube.

3 Put the analyser into the optical path.The analyser will come into the optical pathif the analyser knob on the right side of themicroscope body is set so that the whitemark will match with P

4 By turning the polar izer knob the polarizerand analyser can be made para llel to eachother or cross at right anglesCrossed posit ion: can be obtained when the

polarizer knob is set at OParallel position: can be obtained when the

polarizer knob is set at 90.5 After the observation with polarized light is

over, remove the analyser ou t of the opticalpath b y reversing the procedureJ and pullou t the polarizer. Turn the cover glase ringto cover the window b y reversing theprocedurerjj .

2

Fig 24

Fig 25

Fig 26


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V II haracteristics lenses < , I I I I j e c t i v e Magnification M5X M10X M40X M100X

E P ~ Numerical aperture NA ) 0.10 0.25 0.65 1.30Working distance WD) 27 7. 6 0. 5 0.35Focal lenght f) 31.5 19.6 5. 4 2. 3P7X Total magnif ication 35 X 70X 280X 700X

No. of view Depth of focus 124.6 23.6 2. 5 0. 8field : 18 A ct ua l v ie w field 3. 6 1.8 0.45 0.18

WFlOX Total magnificat ion I 50X I lOOX 400X 1000XNo. of view Depth of focus I 95.5 I 18.0 2. 0 0. 6f ield: 18 Actual view field 3. 6 1.8 0.45 0.18P15X Total magnif ication 75X 150X 600X 1500X

No. of view Depth of focus 72.8 13.5 1.5 0. 5f ield: 9. 5 Actual view field 1. 9 0.95 0.24 0.095

K20X Total magnification lOOX 200X 800X 2000XNo. of viewf ield: 7. 5 Depth of focus 61.5 11.2 1. 3 0. 4 Viewfinder) A ct ua l v ie w field 1.5 0.75 0.19 0.075eyepieceNA : Numerical aperture of objectiveWD : W or ki ng d is ta n cef : Focal length mm)No. of view field : A n um be r w hi ch

repressents in mm the size of th eimage of t he fi el d diaphragmformed by a lens in front of it.

IX Important points to remember

Total magnificaticn : Magnification ofobjective) X Magnification ofeyepiece

Actual view f ield: Field number dividedby the objective magnif ication

Depth of focus : The extent of th edepth of specimen in focus. 1.

Dampness and dust are the w ors t enemies of a microscope. Special care must be taken toprotect t he i ns tru me nt from th e principal trouble makers.

It frequently happens that a research laboratory and other places where microscopes areused have th e above-stated unfavorable conditions. So i t is advisable that when the instrumentis no t in use it is stored in th e case.

If working conditions at the laboratory do no t allow the equipment to be stored a wa y e ve rytim e its job is finished, use th e v in yl covev to protect it from dust.

It is best to store eyepieces and objectives in the dessicator. It is also advisable to placesilicagel dessicating agent) in the container.

When the eyepieces are detached from the microscope eyepiece tubes should be coveredwith their caps.

Strictly avoid disassembling and tinkering with the mechanical parts of the microscope.Leave such work to professional technicians.

Great care must be exerc ised in c lean ing th e instrument.For example when you want to remove dus t from inaccessible parts of the instrument, blowit with a rubber blower or brush i t away with a soft brush.

As we are continuously improving and developing our products, the equipment suppliedma y no t agree in all details with the d es cr ip ti on s a nd /o r i ll us tr at io ns shown in thisbooklet.

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Documents

Olympus Mg Instructions