Olympus Bh Bhm Instructions

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<ul><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 1/20</p><p>OLYMPUS SYSTEM MICROSCOPEFOR MET LLURGIC L USE</p><p>scanned by J . G .M c H on e 6 N o v lfo r persona l u s e only not fo r sa le =INSTRUCTION M NU L MODEL</p><p>. C.</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 2/20</p><p> O N T E N T S</p><p>Vl l l .</p><p>IX</p><p>STANDARD EQUIPMENT . . . . . . . . 2VARIOUS COMPONENTS OF THE MODEL BHM . . . 3ASSEMBLY . . . . . . . . . 4IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS . . 5OPTICAL SYSTEM . . . . . . . . . . 81. Objectives2 E y e p i e c e s . . . . . . . . . . . . . . 93 Vertical Illuminator . . . . . . . . . . . . 11</p><p>A. Aperture iris diaphragm . . . . . . . . . 12B. Field iris diaphragm</p><p>LIGHT SOURCESTAGES . . . . . . . 31. Removalof Specimen Holder2 Stage Spacer3 Metal SlidesOBSERVATION TUBE . . . . . . . . . . 141 lnterpupillary Distance and Diopter Adjustments2 Light Path SelectionFOCUSING ADJUSTMENT . . . . . . . . 151 Tension Adjustment of Coarse Adjustment Knobs2 Automatic Pre focusing Lever3 Stage Height Locking LeverTROUBLESHOOTING . . . . . . . . . 16</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 3/20</p><p>I STANDARD EQUIPMENT</p><p>Component</p><p>Microscope stand with base BHM-F-2Revolving nosepiece BH-RE</p><p>Binocular tube, incl~ned 0 BH-B 130Observation</p><p>BHM</p><p>tubes Trinocular tube, inclined 30, w ~ t h BH-TR30vertical photo tube</p><p>o</p><p>Vertical illuminator for brightfield BH-MA15-watt tungsten lamp house BH-LHMTungsten bulb, 6V 15W, 3 pcs. LS15Square mechanical stage with right-hand low drive controls BH-SVTransformer TE-l I</p><p>o</p><p>b~ectlves</p><p>o</p><p>5X, MIOX, MZOX, M40X (set of four)M Plan 5X, M Plan lox M Plan 20X,M Plan 40X, M Plan 100X (oil) (set of five)</p><p>Eyepieces High eyepoint BiWF lOX, pairedPhoto eyepiece F K3.3XMetal slide plates (set of f~ ve )</p><p>nrnersion oil, bottledEyepiece caps (2 pcs.)Filter 32.5CVinyl dust cover</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 4/20</p><p>I I V RIO US COMPONENTS OF THE MODEL BHM</p><p>The Oiympus System Microscope for reflected light Model BHM consists of a modularbuilding-block system of various components and interchangeable accessories as shownbelow.</p><p>wide variety of combinations standard or optional is available according to yourrequirements.</p><p>Eyepiece.</p><p>Observation tube/C15-watt tungsten</p><p>Vertical illuminator</p><p>Objectivetage</p><p>Cord adapter</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 5/20</p><p>Ill SSEMBLYThe picture below illustrates the sequential procedure of assembly. The numbers indicatethe order of assembly of various components. Remove dust caps before mounting com-ponents. Take care to keep all glass surfaces clean nd avoid scratching the glass surfaces.</p><p>Eyepiece caps</p><p>Observation tube</p><p>Tungsten bc~</p><p>This unit should be attached on the microscope stand with the lamp house pointing awayfrom the observer.</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 6/20</p><p>IV IDENTI FlCATlON AND FUNCTION OF VARIOUS COMPONENTS 0</p><p>Mechanical tube lengthadjustment rings</p><p>arnp house</p><p>Rotate the rings to matchthe interpupillary distance set-ting obt a~n ed rom the scaleand to correct individual diop-ter adjustments.</p><p>control knobs</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 7/20</p><p>lnterpupillary distancescale</p><p>Low voltagement knob</p><p>Pilot lampVoltmeter</p><p>Main switch</p><p>Ana ly~ er otationAperture iris</p><p>Field iris diaphragmHigh low magnificationselector lever</p><p>Filter mountPolarizer mount</p><p>Position L for objective 5 Xand position H for objec-tives 10X and higher.</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 8/20</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 9/20</p><p> V OPTIC L SYSTEMThe optical system of the Series BH is divided into five sections: Objectives, ObservationTubes, Eyepieces, Illuminat ing System and Photomicrographic Equipment, The followingsection deals with objectives, eyepieces and i lluminating system.</p><p>1 ObjectivesA. Types</p><p>Two types of Olympus objectives are mentioned below, in accordance with their opticalperformance and corrections of various aberrations.</p><p>Achromats:Corrected for chromatic aberration, and most popular for general use.Plan achromats:Capable of producing a flat image to the edge of the field. Recommended for visualobservation of a large field and photomicrography o f flat objects.</p><p>B How to UseImmersion objectives (engrave HI for homogeneous immersion):To uti lize the full numerical aperture of an immersion objective, the objective and speci-men are immersed in an Olympus immersion oil, provided. Care should be taken toprevent oi l bubbles from forming in the oi l f i lm between specimen and objective.After use, carefully wipe off the immersion oil deposited on the top lens surface of theobjective wi th gauze moistened with xylene. Take care not to immerse objectives otherthan immersion objectives.Special objectives:</p><p>Long working distance objectives:Provide a longer working distance than standard objectives of equal power.Differential interference contrast objectives:These strain-free objectives of high N.A. can be used not only for the differentialinterference contrast method, but also fo r brightfield.</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 10/20</p><p>2. EyepiecesThe eyepieces available with the Series BH are computed to correct slight residual errorsleft uncorrected in the objectives and designed to further magnify the primary image fromthe objective, l imit ing the field as viewed by the eye.</p><p>Widefield eyepiece WF):Color corrected and f lat , w~de ield; high eyepoint, convenient for observers wearingeyeglasses.Compensating eyepiece K):Corrected for chromatic aberration and astigmatism. For use with high power objectives.Photo eyepiece { F K :For photomicrographic use. Fully corrected for field flatness in combination w ith allOlympus objectives.The eyepieces mentioned above can be used with drop-in eyepiece micrometer discs.</p><p>Q Use of eyepiece cap fo r standard eyepiece)The eyepiece cap is recommended for those who wear eyeglasses. I t prevents damage tothe eyeglasses.</p><p>Q Use of eyepiece wi th eye shieldThe eyepiece WF lOX incorporates a sliding eye shield. This eye shield can be polled outto prevent glare and loss of contrast caused by ambient light hitting the eyepiece frontlens.</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 11/20</p><p> BiK5X and BiWF15X are optionally available.</p><p>is obtained when the objective is used with fully opened ape rture diaphragm.A longer working distance is covenient to</p><p>N.A. A mathematical relationship that directly connects the resolving power and the light-gathering power o fN.A. s the product of the sine of half the angular aperture of a lens, and the refractive index of thes a very important constant for high power lenses. The N.A. values can be used for</p><p>is of vital importance in critical microscopy. The resolving powerR K W a v e y K=cons ant</p><p>is 400mp to 700m p Decreasing the wavelength of the light employed increasesThe higher the resolving power of an objective, the closer the image will be to the true struc ture of the object.the upper and lower limits of sharpness in the image formed by an optical system is termed focaloutsi de these limits are more or less blurred and with low power objectives are apt to interfere with the image inN.A. he larger the focal depth. Lack of focal depth isomicrography, particularly with low power objectives, as the image is projected on the film in one place.the diameter in mm o the image of the field diaphragm that is formed by the lens in front of it.Tllis is derived f rom Field number of eyepiece.'</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 12/20</p><p>3 Vertical Illuminator</p><p>The reflected illumination system adopted in the Model HM is based on the Koehlerprinciple wi th features as follows:</p><p>Aperture iris diaphragm and field iris diaphragm operate effectivelyThe light source illuminates the ful l numerical aperture of the objective.</p><p>The entire field of view can be evenly illuminated.The illuminator is provided with a high/low magnification selector lever to exactlymatch illumination with objective magnification n use.Optimum light intensity can be easily obtained.</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 13/20</p><p>A. Aperture lris DiaphragmIn order to achieve optimum objective performance, the opening of the aperture irisdiaphragm should be matched to the numerical aperture of the objective in use. I t is oftenpreferable, however, to stop down the aperture diaphragm slightly more than indicated bythe objective N.A. This will result in better image contrast, increased depth of focus and aflatter field. After completing focus adjustment, remove one of the eyepieces from theobservation tube and look into the empty eyepiece tube. As you stop down the apertureiris diaphragm, the image of the iris diaphragm can be seen in the objective pupil. Adjust theopening of the diaphragm to match the N.A. of the objective in use. If the specimen is lowin contrast, it IS recommended to stop down to 70-80 of the objective N.A.</p><p>Opening of the di phr gm</p><p>Objective pupil ,--+ \8 Field lris Diaphragm</p><p>The field iris diaphragm controls the diameter o f the ray bundle impinging on the specimensurface and thus increases image definition and reduces glare. For microscopic observation,it is necessary to stop down the field iris diaphragm until the diaphragm s just outside thefield of view as you look through the eyepiece. For photomicrography, the field irisdiaphragm must be stopped down in accordance with the field as viewed through the fieldof view eyepiece, focusing telescope, etc, in use.</p><p>VI LIGHT SOLIRCEThe standard light source incorporates a 15W pre-centered tungsten filament bulb (LS15),combined with a socket for positive contact, eliminating the problems of defective contactand over-heating.When used at the rated voltage 6V, the average life of the tungsten bulb LS15 is longer than200 hours. This is, however, greatly reduced, if the bulb is used at higher voltage; forinstance, the bulb life is reduced to 1/50 a t 8V. Therefore, i t is advisable to avoid prolongeduse at readings over 6V (i n the red zone).If high intensity illumination is required, ~ is recommended to use the optional hidhintensity halogen illuminator,Do not switch the tungsten bulb on at high intensity. I t reduces bulb life.</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 14/20</p><p>VI I STAGES1. Removal of Specimen Holder</p><p>1 Square mechanical s tage wi th r ight-hand low d r ive con-t rols Model BH-SVThe standard mechanical stage is provid ed wi th a spring-loaded specimen holder, which is capable of holdingspecimens up t o 55rrtm x 85 mm in size. The specimenholder is removable t o obta in a large uno bstrt icted stagesurface, Fig. 1)</p><p>2) Large mechanical stage Mod el B H-SICThis 20Omm x 161m m square mechanical stage has atraversing area o f 11Omm x 100mm , by rneans of coax iallow drive contro ls. I t is possible t o adjust the distancebetween the holders according to specimen sizes, Forexcept ional ly large specimens, the holders cart be re-moved. For use with IC wafers, masks or other specialspecimens, custonier designed specimen holders can beu t i l i i ed .</p><p>2. Stage SpacerIn order to prevent interference between objectives andspecimen holder it is recom men ded t o use the stage spacerp ro vi de d w ~ t t i he stage Model BH-SV and place i t in themanner shown in F ig. 3, pr ior to plac ing a th in spec imen o n</p><p>IFig. 1</p><p>th e stage. Fig . 33. Meta l Slides</p><p>T o level a specimen:1) Put plastic ine on a meta l slide supplied.2 i Place a specimen on the plasticine, and press the speci-</p><p>men with a hand press unti l the specimen surface is Fiq. 3properly leveled.</p><p>It is suggested to place a piece of tissue paper betweenthe specimen and the plunger of the hand press i n orderto prevent scratching of th e polished specimen surface.F ig . 4)</p><p>Fig. 4</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 15/20</p><p> OBSERV TION TUBElnterpupillary Distance and Diopter Adjustments1 ) Hold the knurled dovetail slides @ of the right and left</p><p>eyepiece tubes with both hands and push the tubestogether, or pull them apart laterally, whichever isrequired, while looking through the eyepieces with botheyes, until perfect binccular vision is obtained.</p><p>2) Memorize your interpupillary distance setting. Scale @Jis provided for this purpose. Fig. 5</p><p>3 Rotate the tube length adjustment ring @ on the right eyepiece tube to match yourinterpupillary distance setting which you obtained from the scale. (Fig. 5</p><p>4 Look at the image through the right eyepiece wi th your right eye and focus on the speci-men with the fine adjustment knobs.</p><p>5 Next, look at the image through the left eyepiece with your left eye and rotate the tubelength adjustment ring @ to focus on the specimen without using the coarse and fineadjustment knobs.* The mechanical tube length of the Olympus metallurgical microscope is standardizedat 200mm.</p><p>2. Light Path SelectionThe trinocular observation tube is provided wi th a light path selector lever to direct the lightto the observation tube or to the photo tube,</p><p>* In case of long time exposure, it is necessary to darken the room or put a pair of light shieldcaps, provided with the trinocular tube, on the eyepiece tubes, in order to prevent light fromcoming on the fi lm plane.</p><p>Lever positionPushed in all the way</p><p>Pulled out all the way</p><p>Amount of light100 in to binocular tube</p><p>20 into binocular tube80 into photo tube</p><p>Application1 ) Normal observation12) Dark specimens1 Photomicrography2 ) Observation of excessively</p><p>bright specimens -</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 16/20</p></li><li><p>8/12/2019 Olympus Bh Bhm Instructions</p><p> 17/20</p><p>X TROU LESHOOTING</p><p>b)The field o f view is cutoff or illuminated ir-regularly.</p><p>Troubles1 Optical System</p><p>The light path selector lever isstopped midway.</p><p>a]With the illuminatorswitched on, the fieldof view cannot be seen.</p><p>Push the lever all the way.</p><p>Causes</p><p>The nosepiece is not clicked intoposition.</p><p>Remedies</p><p>The field iris diaphragm is notopened sufficiently.</p><p>Slightly rotate the nosepiece unti lit clicks into position.</p><p>Open the field diaphragm fully.</p><p>The field iris diaphragm is stoppeddown excessive1y.</p><p>Open the diaphragm sufficient ly</p><p>The bulb or lamp house is not cor-rectly positioned.The highilow magnification selectorlever of the illuminator is not cor-rectly positioned.</p><p>Insert the bulb or lamp house allthe way.Position the lever correctly.</p><p>I c)Dust or di rt is visible in Dust or dirt on the bulb end.Di rty specimen. Clean of f the dust or dirt.Dust on eyepiece.</p><p>the f ie d of view. Dust on half mirror.</p><p>prism in the observation tube.d)Excessive image con-</p><p>trast. ped down excessively.</p><p>Dust on the lower surface of the</p><p>e) Resolution problems:Image is not sharp.2 Insufficient contrast.</p><p>The highilow magnification selectorlever is not correctly positioned.The bulb or lamp house is notcorrectly positioned.The objective is not correctly posi-tioned in the light path.Di rt on objective front lens.The immersion objective is usedwithout immersion oil.Bubbles in the immersion oil.The Olyinpus specified oil is notused.The specimen is not properly il-luminated.</p><p>Open the diaphragm.</p><p>Place the lever in correct position.</p><p>Push the bulb or lamp house ail theway home.Slightly rotate the nosepiece untili t clicks in to position.Clean the objective.Apply immersion oil.</p><p>Remov...</p></li></ul>


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