1

Nuclear DNA (nDNA) Mitochondrial DNA (mtDNA) - Found in the nucleus of the cell - 3,300,000,000 bases (A, T, C and G) - Arranged in linear chromosomes - Two copies of each chromosome - Maternally and paternally inherited - Contains approximately 20,000 genes - 93% of the genome is non-coding DNA - Found in the mitochondrial matrix - 16,569 bases (A, T, C and G) - Arranged in a circular chain of DNA - Multiple copies present - Maternally inherited - Contains 37 genes - 3% of the genome is non-coding DNA Mendelian inheritance Non-Mendelian inheritance Mother: ‘healthy’ carrier Father: ‘healthy’ carrier ‘healthy’ child: 1 in 4 chance ‘healthy’ carrier child: 2 in 4 chance ‘diseased’ child: 1 in 4 chance - Example of an autosomal recessive disease - Disease severity is correlated to ratio of healthy to diseased mitochondria Healthy mitochondria Low level of mutation: healthy child Intermediate level of mutation: mild disease High level of mutation: severe disease mother cell oocytes Diseased mitochondria with mutated mtDNA Nucleus Nuclear DNA v mtDNA: principles of inheritance

Nuclear DNA v mtDNA: principles of inheritanceNuclear DNA v mtDNA: principles of inheritance 11 17 22 13 14 15 20 10 16 12 18 Normal Karyotype MRC Mitochondrial Biology Unit Created

Download PDF Report

Upload
others
View
3
Download
0

Embed Size (px)

Citation preview

Page 1: Nuclear DNA v mtDNA: principles of inheritanceNuclear DNA v mtDNA: principles of inheritance 11 17 22 13 14 15 20 10 16 12 18 Normal Karyotype MRC Mitochondrial Biology Unit Created

NuclearDNA(nDNA) MitochondrialDNA(mtDNA)

- Foundinthenucleusofthecell- 3,300,000,000bases(A,T,CandG)- Arrangedinlinearchromosomes- Twocopiesofeachchromosome- Maternallyandpaternallyinherited- Containsapproximately20,000genes- 93%ofthegenomeisnon-codingDNA

- Foundinthemitochondrialmatrix- 16,569bases(A,T,CandG)- ArrangedinacircularchainofDNA- Multiplecopiespresent- Maternallyinherited- Contains37genes- 3%ofthegenomeisnon-codingDNA

Mendelianinheritance Non-Mendelianinheritance

Mother:‘healthy’carrier

Father:‘healthy’carrier

‘healthy’child:1in4chance

‘healthy’carrierchild:

2in4chance

‘diseased’child:1in4chance

- Exampleofanautosomalrecessivedisease

- Diseaseseverityiscorrelatedtoratioofhealthytodiseasedmitochondria

Healthymitochondria

Lowlevelofmutation:healthychild

Intermediatelevelofmutation:milddisease

Highlevelofmutation:severedisease

mothercell

oocytes

DiseasedmitochondriawithmutatedmtDNA

Nucleus

NuclearDNAvmtDNA:principlesofinheritance

Buku Referensirepository.unair.ac.id/99577/1/forensik rev.pdfBuku Referensi Pemeriksaan Forensik DNA Tulang dan Gigi : Identifikasi pada DNA Lokus STR CODIS, Y-STRs, dan mtDNA Dr

Buku Referensirepository.unair.ac.id/99577/1/forensik rev.pdfBuku Referensi Pemeriksaan Forensik DNA Tulang dan Gigi : Identifikasi pada DNA Lokus STR CODIS, Y-STRs, dan mtDNA Dr

Documents

D DNA (mtDNA) M -J

D DNA (mtDNA) M -J

Documents

Allozyme, mtDNA, and microsatellite variants describe ... · mitochondrial DNA (mtDNA) to solve questions of population structure. We also report pilot studies to collect data on

Allozyme, mtDNA, and microsatellite variants describe ... · mitochondrial DNA (mtDNA) to solve questions of population structure. We also report pilot studies to collect data on

Documents

PROSIDING - repository.uksw.edurepository.uksw.edu/bitstream/123456789/16166/1/PROS_Lois BL... · are performed using a molecular genetic marker of mitochondrial DNA (mtDNA), i.e

PROSIDING - repository.uksw.edurepository.uksw.edu/bitstream/123456789/16166/1/PROS_Lois BL... · are performed using a molecular genetic marker of mitochondrial DNA (mtDNA), i.e

Documents

What is Mitochondrial DNA (mtDNA)?

What is Mitochondrial DNA (mtDNA)?

Documents

Ancient DNA 5 - Ziggomembers.chello.nl/jperk/Ancient DNA 5.pdf · 6 Ancient DNA analysis of art and artefacts Chair: Keri Brown 1400 – 1430 Joachim Burger - STR profiles, mtDNA

Ancient DNA 5 - Ziggomembers.chello.nl/jperk/Ancient DNA 5.pdf · 6 Ancient DNA analysis of art and artefacts Chair: Keri Brown 1400 – 1430 Joachim Burger - STR profiles, mtDNA

Documents

DNA: Review, Replication, & Analysis Two types of DNA Nucleic DNA –Found in the nucleus of a cell –Specific to an individual Mitochondrial DNA (mtDNA)

DNA: Review, Replication, & Analysis Two types of DNA Nucleic DNA –Found in the nucleus of a cell –Specific to an individual Mitochondrial DNA (mtDNA)

Documents

Regulation of nuclear epigenome by mitochondrial DNA ...Mitochondrial DNA (mtDNA) mutations have been associated with common metabolic and degenerative disease phenotypes, implying

Regulation of nuclear epigenome by mitochondrial DNA ...Mitochondrial DNA (mtDNA) mutations have been associated with common metabolic and degenerative disease phenotypes, implying

Documents

Organization and dynamics of human mitochondrial DNA · They contain their own genome, the mitochondrial DNA (mtDNA), which encodes a limited number of essential mitochondrial proteins

Organization and dynamics of human mitochondrial DNA · They contain their own genome, the mitochondrial DNA (mtDNA), which encodes a limited number of essential mitochondrial proteins

Documents

Mitochondrial DNA and EMPOP - isfg.org · Mitochondrial DNA and EMPOP Dr. Walther Parson ... Fendt et al 2009) MtDNA amplification and sequencing W. Parson – Mitochondrial DNA and

Mitochondrial DNA and EMPOP - isfg.org · Mitochondrial DNA and EMPOP Dr. Walther Parson ... Fendt et al 2009) MtDNA amplification and sequencing W. Parson – Mitochondrial DNA and

Documents

Teknik Analisis Non-Invasif Mitokondria DNA ( MtDNA) BILOU

Teknik Analisis Non-Invasif Mitokondria DNA ( MtDNA) BILOU

Documents

DNA Mitocondrial (mtDNA) y Evolución Humana

DNA Mitocondrial (mtDNA) y Evolución Humana

Documents

Porcine Dna Detection in Finished Meat Products Using Different Mitochondrial Dna Mtdna on Polymerase Chain Reaction 2155 9600.1000323 4

Porcine Dna Detection in Finished Meat Products Using Different Mitochondrial Dna Mtdna on Polymerase Chain Reaction 2155 9600.1000323 4

Documents

D DNA (mtDNA) M -J - core.ac.uk · direta ou indiretamente, na realização deste trabalho. No entanto, gostaria de expressar um sincero agradecimento: ... DNA mitocondrial (mtDNA)

D DNA (mtDNA) M -J - core.ac.uk · direta ou indiretamente, na realização deste trabalho. No entanto, gostaria de expressar um sincero agradecimento: ... DNA mitocondrial (mtDNA)

Documents

Human variation – DNA profiling with mtDNA and … DNAprofiling.pdfHuman variation – DNA profiling with mtDNA and microsatellites Eric Yap Defence Medical Research Institute Singapore

Human variation – DNA profiling with mtDNA and … DNAprofiling.pdfHuman variation – DNA profiling with mtDNA and microsatellites Eric Yap Defence Medical Research Institute Singapore

Documents

Complete DNA Sequence of the Mitochondrial …The DNA sequence of the 15,532-base pair (bp) mitochondrial DNA (mtDNA) of the chiton Katharina tunicata has been determined. The 37 genes

Complete DNA Sequence of the Mitochondrial …The DNA sequence of the 15,532-base pair (bp) mitochondrial DNA (mtDNA) of the chiton Katharina tunicata has been determined. The 37 genes

Documents

AGING PROTOCOL - DaVinci Labs · AGING PROTOCOL Viscious Cycle MtDNA damage Electron transfer chain dysfunction Subunit coded by MtDNA Nuclear DNA ATP ... Phyto Benefits™: 1 scoop

AGING PROTOCOL - DaVinci Labs · AGING PROTOCOL Viscious Cycle MtDNA damage Electron transfer chain dysfunction Subunit coded by MtDNA Nuclear DNA ATP ... Phyto Benefits™: 1 scoop

Documents

Dual Functions of -Ketoglutarate Dehydrogenase E2 in the ... · the mitochondrial DNA (mtDNA) of multiple eukaryotic organ-isms (4–9). mtDNA is organized into stable protein-DNA

Dual Functions of -Ketoglutarate Dehydrogenase E2 in the ... · the mitochondrial DNA (mtDNA) of multiple eukaryotic organ-isms (4–9). mtDNA is organized into stable protein-DNA

Documents

Nuclear DNA v mtDNA: Principles of inheritance · - 16,569 bases in a circular chain of DNA - Multiple copies present in each mitochondrion - Maternally inherited - Contains 37 genes

Nuclear DNA v mtDNA: Principles of inheritance · - 16,569 bases in a circular chain of DNA - Multiple copies present in each mitochondrion - Maternally inherited - Contains 37 genes

Documents

Restriction fragment length polymorphisms of mitochondrial ... · Restriction fragment length polymorphism (RFLP) analysis of mitochondrial DNA (mtDNA) was employed to characterize

Restriction fragment length polymorphisms of mitochondrial ... · Restriction fragment length polymorphism (RFLP) analysis of mitochondrial DNA (mtDNA) was employed to characterize

Documents

Mitochondrial DNA mutationslifeserv.bgu.ac.il/wb/dmishmar/media/p68-70_Dan_Mishmar.pdf · This mitochondrial DNA (mtDNA) contains genes that are vital for the functioning of the mitochondrion,

Mitochondrial DNA mutationslifeserv.bgu.ac.il/wb/dmishmar/media/p68-70_Dan_Mishmar.pdf · This mitochondrial DNA (mtDNA) contains genes that are vital for the functioning of the mitochondrion,

Documents

Principles of DNA Sequencing - Assiut University DNA Seq... · Principles of DNA Sequencing Dr. Serageldeen A. A. Sultan PhD in Molecular virology Yamaguchi University, Japan (2010)

Principles of DNA Sequencing - Assiut University DNA Seq... · Principles of DNA Sequencing Dr. Serageldeen A. A. Sultan PhD in Molecular virology Yamaguchi University, Japan (2010)

Documents

Derived immune and ancestral pigmentation alleles in a ... de ojos azules y piel oscura... · DNA(mtDNA)andthenuclearDNAofLaBran˜a1showedthetypical ancientDNAmisincorporationpatternsthatarisefromdegradationof

Derived immune and ancestral pigmentation alleles in a ... de ojos azules y piel oscura... · DNA(mtDNA)andthenuclearDNAofLaBran˜a1showedthetypical ancientDNAmisincorporationpatternsthatarisefromdegradationof

Documents

Testing Mitochondrial DNA and Mitochondrial …...mitochondrial disorders involve both mitochondrial and nuclear DNA, some syndromes are associated specifically with mtDNA mutations

Testing Mitochondrial DNA and Mitochondrial …...mitochondrial disorders involve both mitochondrial and nuclear DNA, some syndromes are associated specifically with mtDNA mutations

Documents

Forensic Casework Applications of mtDNA · • Modern DNA can easily be introduced and overwhelm target DNA from the sample. – Due to the sensitivity of the reaction • Increased

Forensic Casework Applications of mtDNA · • Modern DNA can easily be introduced and overwhelm target DNA from the sample. – Due to the sensitivity of the reaction • Increased

Documents

DNA MUTATION DETECTION - science.iautmu.ac.irscience.iautmu.ac.ir/file/download/page/1583216014-.pdf · MITOCHONDRIAL DNA(MT DNA) mtDNA is a double stranded circular molecule. mtDNA

DNA MUTATION DETECTION - science.iautmu.ac.irscience.iautmu.ac.ir/file/download/page/1583216014-.pdf · MITOCHONDRIAL DNA(MT DNA) mtDNA is a double stranded circular molecule. mtDNA

Documents

Principles of Dna Isolation & Purification

Principles of Dna Isolation & Purification

Documents

Mitochondrial DNA analysis in primary congenital · PDF fileMitochondrial DNA analysis in primary congenital glaucoma ... This may lead to impaired growth, development and ... MtDNA

Mitochondrial DNA analysis in primary congenital · PDF fileMitochondrial DNA analysis in primary congenital glaucoma ... This may lead to impaired growth, development and ... MtDNA

Documents

Cancio, Fosgate, Ramos, de Castro, Gatchalian, Serrano ... · Cancio, Fosgate, Ramos, de Castro, Gatchalian, Serrano. DNA Probe ... Mitochondrial DNA (mtDNA) vs Nuclear DNA-far smaller

Cancio, Fosgate, Ramos, de Castro, Gatchalian, Serrano ... · Cancio, Fosgate, Ramos, de Castro, Gatchalian, Serrano. DNA Probe ... Mitochondrial DNA (mtDNA) vs Nuclear DNA-far smaller

Documents

CHLOROPLAST DNA DIVERSIFICATION OF MALAYSIAN … · seperti DNA Mitokondria (mtDNA), DNA Kloroplas (cpDNA) dan DNA Nuklear (nDNA). Antara ketiga-tiga genom ini, cpDNA banyak digunakan

CHLOROPLAST DNA DIVERSIFICATION OF MALAYSIAN … · seperti DNA Mitokondria (mtDNA), DNA Kloroplas (cpDNA) dan DNA Nuklear (nDNA). Antara ketiga-tiga genom ini, cpDNA banyak digunakan

Documents