7WWW.CEN-ONLINE.ORG OCTOBER 13, 2008

THE 2008 NOBEL PRIZE in Chemistry honors three scientists for the discovery and develop-ment of green fluorescent protein (GFP). This

protein and mutated versions of it have become power-ful tools for tagging and observing other proteins in cells. Osamu Shimomura, Martin Chalfie, and Roger Y. Tsien will equally share the $1.4 million prize.

GFP has been “just as useful as the invention of the microscope,” says Marc Zimmer, a chemistry profes-

sor at Connecticut College who has written a book about GFP. “It lets us see things we’ve never been able to see before.”

Shimomura, 80, senior scientist emeritus at the Marine Biological Laboratory, in Woods Hole, Mass., was the first person to isolate GFP from the jellyfish Aequorea victoria. Shimomura, working in Frank H. Johnson’s lab at Princeton University, identified aequorin as the active component of Aequorea bio-luminescence. Aequorin, however, emits blue light, so he knew there must be another protein involved because the jellyfish’s fluorescence is distinctly green. He and his coworkers searched for such a protein and found GFP, which fluoresces green when it absorbs the blue light emitted by aequorin.

Chalfie, 61, a professor of biological sciences at Co-lumbia University, pioneered the use of GFP as a fluo-rescent label inside living organisms. He genetically ma-nipulated GFP to create fusion proteins, in which GFP is linked to other proteins. These GFP-tagged proteins can

be expressed in other organisms such as the roundworm Caenorhabditis elegans and the bacterium Escherichia coli. The pro-teins glow when they are expressed, making them easy to track in cells.

Tsien, 56, a professor of pharmacol-ogy at the University of California, San Diego, showed how the GFP chromophore assumes its shape. GFP forms an 11-strand-ed β-barrel. The three amino acids that constitute the chromophore are located in an α-helix that threads itself through the center of the barrel. The amide of a glycine on this helix reacts with the carbonyl of a nearby serine to form an imidazoli-none. The chromophore becomes fluo-rescent only in the presence of oxygen, which causes a third amino acid to be dehydrogenated and conjugated to the

imidazolinone.Using this structural and

mechanistic knowledge, Tsien genetically engineered GFP mu-tants that absorb and emit light in other spectral regions. He has created a veritable rainbow of fluorescent proteins that allow the simultaneous labeling of multiple proteins inside cells.

Tsien’s early GFP work “was made possible by the DNA that we received as a generous gift from Douglas Prasher,” Tsien said at a press conference.

Prasher, who sequenced and cloned GFP, provided DNA to both Chalfie and Tsien.

“The impact of GFP has been enor-mous,” says Jeremy M. Berg, director of the National Institute of General Medical Sciences at NIH. The protein has become an integral part of “the fabric of modern cell biology,” he says.

“It’s hard to open a biological journal these days without seeing some article where GFP was used in a clever way to reveal the inner workings of living cells,” says George N. Phillips Jr., a biochemistry professor at the University of Wisconsin, Madison, whose team solved the crystal structure of GFP.

Bruce E. Bursten, president of the American Chemi-cal Society, notes that the prize “showcases chemistry’s critical but often invisible role in fostering advances in biology and medicine.”—CELIA ARNAUD

Engineered variations in GFP result in a rainbow of fluorescent proteins.

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NOBEL PRIZE IN CHEMISTRY

AWARDS: Japanese, two Americans honored for their work with

green fluorescent protein

news of the weekOCTOBER 13, 2008 EDITED BY WILLIAM G. SCHULZ & KENNETH J. MOORE

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Conceptual rendering of a fusion of GFP and a peptide.