NEW$MICROALGAE$ BIOREFINERY$CONCEPT ... Spent microalgae post transesterification 1 Spent microalgae

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  • 1  1  

    NEW  MICROALGAE   BIOREFINERY  CONCEPT  

       Laura  Azócar,  Hernán  Díaz,  Robinson  Muñoz,   Leonel  González,  David  Jeison,  Rodrigo  

    Navia  

    Financiado  por  

  • 2  

    IntroducEon  

    Lam  and  Lee,  2012  

    Brennan  and  Owende,  2010  

  • Downstream  

    3  

    Mata  et  al.  2012  

    Ahmad  et  al.  2012  

    Brennan  and  Owende,  2010  

  • Downstream  

    Waste  biomass  (70%)   Solvents  

    Harvest  

    Biomass  procesing    

    Lipid  extracEon  

    TransesterificaEon  

    Protein  extracEon  

    Anaerobic  digesEon  

    Biodiesel  upgrading    

    Harvest  

    Carbohidrate  extracEon  to   bioethanol  producEon  

    Direct  transesterificaEon  

    Protein  extracEon  

    Anaerobic  digesEon  

    Biodiesel  upgrading    

  • 5  

    SequenEal  opEmizaEon    

    Variables   pH=  9-­‐10-­‐11-­‐  12-­‐13  

    Temperature  (°C)=  30-­‐50-­‐70   Eme  (min)=  10-­‐  20-­‐  30    

    SEr  (rpm)=  100-­‐  200-­‐  300    

    Chemical  proteins  solubilizaEon  &  EnzymaEc  hydrolysis    

    PROTEIN  EXTRACTION  

    CHEMICAL  PROTEIN     SOLUBILIZATION  

  • 6  

    pH

    9 10 11 12 13

    % P

    ro te

    in s

    ol ub

    ilit y

    0

    10

    20

    30

    40

    50

    temperature [°C]

    20 30 40 50 60 70

    % P

    ro te

    in s

    ol ub

    ilit y

    0

    10

    20

    30

    40

    50

    60

    Results   ü 50°C   ü 20min     ü 200  rpm  

    ü pH  12   ü 20min     ü 200  rpm  

    Figure   1.   %   Protein   solubility   from  microalgae   biomass  to  different  pH  condiEons      

    Figure   2.   %   Protein   solubility   from   microalgae   biomass  to  different  reacEon  temperature      

  • time [min]

    10 20 30 40 50 60

    % P

    ro te

    in s

    ol ub

    ilit y

    0

    10

    20

    30

    40

    50

    60

    70

    Stir [rpm]

    50 100 150 200 250 300 350

    % P

    ro te

    in s

    ol ub

    ilit y

    0

    20

    40

    60

    80

    ü pH  12   ü 50°C     ü 200  rpm  

    ü pH  12   ü 50°C     ü 40  min  

    OpEmal  condiEons:   pH  12   50°C     40  min   200rpm     Yield:  64%  

    Figure   3.   %   Protein   solubility   from   microalgae   biomass  to  different  reacEon  Eme        

    Figure  4.  %  Protein  solubility  from  microalgae  biomass   to  different  sEr  condiEons  

  • 8  

      1)  EnzymaEc   hydrolysis   of   microalgae   biomass   through   successive   steps  

    addiEon  of:  Viscozyme,  Alcalasa  and  Flavourzyme     2)    EnzymaEc  hydrolysis  of  solubilized  protein  by  chemical  treatment  by  using  

    Alcalasa.     3)  EnzymaEc  hydrolysis  of  solubilized  protein  by  chemical  treatment  by  using  

    Flavourzyme.     Raw  material     1)  Pretreated  Microalgae   150g/L  (similar  to  biomass  post  harvest)      

    ENZYMATIC  HYDROLYSIS  

  • 9  

    Reactor   On  line  traking  Thermoregulated  bath  

    Set  up  

  • EnzymaEc  hydrolysis  of  microalgae  biomass  

    10  

    Time [h]

    0 50 100 150 200 250 300

    % H

    yd ro

    ly si

    s de

    gr ee

    30

    40

    50

    60

    70

    80

    90 Disadvantage   Carbohydrates  loss   (65%)  

    Hydrolysis  degree:  84%    

    Viscozyme   Alcalasa  

    Flavourzyme  

    Figure   5.   Hydrolysis   degree   aner   enzymaEc   hydrolysis   of   microalgae  biomass  

  • Flavourzyme  

    Time [h]

    0 20 40 60 80 100

    H yd

    ro ly

    si s

    de gr

    ee [%

    ]

    20

    30

    40

    50

    60

    70

    80

    90

    Time [h]

    0 50 100 150 200 250 300

    H yd

    ro ly

    si s

    de gr

    ee (%

    )

    40

    50

    60

    70

    80

    90

    Flavourzyme  

    Alcalasa   EnzymaEc  hydrolysis  of   previously  solubilized   protein  by  chemical   process  

    Low  carbohydrates  loss       Carbohydrates  extracEon  to   bioethanol  producEon???  

    Total  yield:  88%    

    Figure   6.   Hydrolysis   degree   aner   enzymaEc   hydrolysis  of  solubilized  protein  by  chemical  process  

    Figure  7.  Hydrolysis  degree  aner  enzymaEc  hydrolysis   of  solubilized  protein  by  chemical  process  

  • 12  Biomass  to  biodiesel  producEon  (high  lipid  content)  

    Carbohydrates  to  bioethanol  producEon  

    CARBOHYDRATES  EXTRACTION  TO  BIOETHANOL  PRODUCTION  

  • BIODIESEL  PRODUCTION  

    Lipids  extracIon  and  transesterificaIon  

    Figure   8.   Chromatogram   faqy   acid   profile   (a)   microalgae  1  and  (b)  microalgae  2    

    (a)  

    (b)  

  • 14  

    BIODIESEL  PRODUCTION  

    Direct  transesterificaIon  

    CondiEons  invesEgated  RSM     ü Methanol  to  oil  molar  raEo   ü Catalyst   ü Time   ü Temperature  

    Figure  9.  Biodiesel  produced  by  direct  transesterificaEon  

  • 15  

    BIOGAS  PRODUCTION  

    Figure  10.  Biomass  during  microalgae  biorefinery  process  (a)Protein    extracEon,  (b)  Drying,   (c)   Dry   biomass,   (d)   Biomass   milled   to   the     lipids   extracEon,   (e)   Biomass   aner   lipids   extracEon,  (f)  Biomass  milled  to  biogas  producEon    

    Biochemical  methane  potenIal  by  using  microalgae  biomass     aQer  lipids  extracIon  

  • 16  

    BIOGAS  PRODUCTION   Biochemical  methane  potenIal  by  using  microalgae  

    biomass  aQer  transesterificaIon  reacIon  

    Figure  10.  Biomass  aner  transesterificaEon  reacEon  

    117  serum  boqles   50  mL  reacEon   Nutrients   Bicarbonate   35°C    

    Assays  

  • 17  

    BIOGAS  PRODUCTION  

    Parámetro Microalgae 1 Spent microalgae 1 Spent microalgae 2 Spent microalgae 2

    Humidity content [%] 79,58 7,66 3,99 3,13 Lipids [%]* 19,20 5,23 9,61 7,4 Protein [%]* 33,00 22,80 39,42 41 Fiber [%]* 3,33 7,94 4,34 3,54 Ash [%]* 31,00 40,13 10,34 13,75 Carbohidrates [%] 13,47 16,24 32,3 31,18 C/N [gC/gN] 5.85 6.97 - -

    *dry  basis  

    Table  1.  Microalgae  characterizaEon  

  • 18  

    BIOGAS  PRODUCTION  

    Time [d]

    0 20 40 60 80

    B io

    ch em

    ic al

    m et

    ha ne

    p ot

    en tia

    l B M

    P [m

    L C

    H 4/g

    V S

    ]

    0

    50

    100

    150

    200

    250

    300

    350

    Spent microalgae post transesterification 1 Spent microalgae post transesterification 2

    Figure   12.   BMP   using   spent   microalgae   aner   direct   transesterificaEon  

    Time [d]

    0 20 40 60 80

    B io

    ch em

    ic al

    m et

    ha ne

    p ot

    en tia

    l B M

    P [m

    L C

    H 4/g

    V S

    ]

    0

    100

    200

    300

    400

    500

    Spent microalgae + residual culture medium Spent microalgae + water + nutrients

    Figure  11.  BMP  using  spent  microalgae  aner   protein  and  lipids  extracEon    

  • 19  

    BIOGAS  PRODUCTION  

    Figure  13.  TheoreEcal  and  real  BMP  usiing  spent  microalgae  

    Microalga agotada Microa

    lgae 1 .1

    Microa lgae 1