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New Analytics for New Insights
Laboratory Proficiency Results FELC 2016
Kristi PlackChief Operations Officer
Bion Companies
New Analytics for New Insights
Novel Compositional Analysis Test Methods Can Shed New Light on Process
Efficiency and Co-Product Quality
Traditional Proximates
Moisture NFTA 2.2.2.5Protein AOAC 990.03
Fat AOAC 945.16Ash AOAC 942.05
Starch Modified MegazymeFiber Ankom NDF
Mass Closure with Traditional Proximates
35.4
31.9
7.85.2
1.6
18.2
0.0
10.0
20.0
30.0
40.0
50.0
60.0
70.0
80.0
90.0
100.0
110.0
Mass Closure byTraditional Proximates
Mass Closure on DDGS (% dw)
Unknown
Starch
Ash
Fat
Protein
Fiber8.4
8.74.2
1.4
75.3
2.0
0.0
10.0
20.0
30.0
40.0
50.0
60.0
70.0
80.0
90.0
100.0
110.0
Mass Closure byTraditional Proximates
Mass Closure on Corn (% dw)
Unknown
Starch
Ash
Fat
Protein
Fiber
Moisture Modified NFTA 2.2.2.5Protein AOAC 990.03
Fat AOAC 945.16Ash AOAC 942.05
Starch Modified MegazymeFiber Ankom NDF
Soluble Saccharides Soliton ProprietaryNon Saccharide Soluble Organics Soliton Proprietary
New Analytics
8.4
8.74.2
1.4
75.3
2.0
0.0
10.0
20.0
30.0
40.0
50.0
60.0
70.0
80.0
90.0
100.0
110.0
Mass Closure byTraditional Proximates
Mass Closure on Corn (% dw)
Unknown
Starch
Ash
Fat
Protein
Fiber 8.4
8.74.2
1.4
75.3
0.3
1.8
0.0
10.0
20.0
30.0
40.0
50.0
60.0
70.0
80.0
90.0
100.0
110.0
Mass Closure by SolitonProprietary Analytics
Mass Closure on Corn (% dw)
Unknown
Non SaccharideSoluble Organics
Starch
Ash
Fat
Protein
Fiber
Mass Closure with New Analytics
When measuring Starch on pre-processed substrates (e.g. Corn) the Starch value includes all soluble C6 carbohydrates, therefore, the SS would be redundant if included in the total mass closure calculation
Mass Closure with New Analytics
35.4
31.9
7.85.2
1.6
18.2
0.0
10.0
20.0
30.0
40.0
50.0
60.0
70.0
80.0
90.0
100.0
110.0
Mass Closure byTraditional Proximates
Mass Closure on DDGS (% dw)
Unknown
Starch
Ash
Fat
Protein
Fiber
35.4
31.9
7.85.2
1.67.9
10.70.0
0.0
10.0
20.0
30.0
40.0
50.0
60.0
70.0
80.0
90.0
100.0
110.0
Mass Closure by SolitonProprietary Analytics
Mass Closure on DDGS (% dw)
Unknown
Non SaccharideSoluble OrganicsSolubleSaccharidesStarch
Ash
Fat
Protein
Fiber
When measuring Starch on post-processed substrates (e.g. DDGS) the Starch value does not include any soluble carbohydrates, therefore, the SS needs to be included in the total mass closure calculation
Soluble Saccharides (SS)
o A technique that measures soluble sugars via complete
analytical speciation and then aggregates the total on a dry
weight basis
Non Saccharide Soluble Organics (NSSO)
o A technique that measures soluble organics via complete
analytical speciation and then aggregates the total on a dry
weight basis
Proprietary Mass Closure Analytics
Mass Closure on Pre-Processed Material (e.g. Corn)
Total = Protein + Fat + Ash + 1Starch + Fiber + NSSO
Mass Closure on Post-Processed Material (e.g. DDGS)
Total = Protein + Fat + Ash + 2Starch + Fiber + 2SS + NSSO
Footnotes:1 When measuring Starch on pre-processed substrates (e.g. Corn) the Starch value includes all soluble C6 carbohydrates, therefore, the SS would be redundant if included in the total mass closure calculation2 When measuring Starch on post-processed substrates (e.g. DDGS) the Starch value does not include any soluble carbohydrates, therefore, the SS needs to be included in the total mass closure calculation
Proprietary Mass Closure Analytics
Transition……
Inaccuracy/Error
There is always some level of inaccuracy
• Reduce• Consequences of• Lab system
QC Goal--Challenge
• Detect
• Evaluate
• Correct errors• System failure, instrument error, environmental
conditions, SOPs not followed or human performance
Assessment
Internal or External
Standards Benchmarks
Proficiency Testing
Laboratory Proficiency Testing
Assessment activity to demonstrate work competence
Evaluation of participant performance against pre-established criteria by means of interlaboratorycomparisons A surveillance activity for assessing the quality
and uniformity of tests and measurements performed in a lab
Laboratory Proficiency Testing
• Triannual Process Testing Proficiency Samples
• Constant changes• Treat PT samples like routine samples• Over 60 Participants• Multiple Results • Different Instruments, personnel
Why Participate in LPT?
• Provide early warning signs of systematic problems
• Provide objective evidence of testing quality
• Indicate areas that need improvement
• Cost effective
• Used for continual improvement
• Identify interlaboratorydifferences
What if my data falls outside acceptance criteria
• Investigate• Review original PT answer sheet/bench
records and look for transcription errors, transposition of answers, miscalculations
• Review QC results from the date the PT results were accepted--- Was QC all in spec?
• Retest original PT sample (if possible)• If retest produces an acceptable result then the original result was
affected by analytical error
Laboratory Proficiency Testing
Analyte/Method Description
July 2015 Group % Recovery (from TV)
Feb 2016 Group % Recovery (from TV)
July 2016 Group % Recovery (from TV)
HPLC Ethanol 99.30 99.4 100.2HPLC Maltotriose 99.17 99.8 93.3HPLC DP4+ 100.93 100.2 108.3HPLC Glycerol 98.39 99.9 99.4HPLC Acetic Acid 97.56 98.6 96.5HPLC Maltose 97.37 99.8 100.8HPLC Glucose 97.12 100.0 102.8
HPLC Lactic Acid 94.7 98.3 98.6
Laboratory Proficiency Testing
Laboratory Proficiency Testing--Benefits
• Monitor and improve measurement• Demonstrate analytical competence• Method and instrument validation• A regular, external and independent check on data quality• Provides laboratories with a means to assess the quality and
accuracy of their results as compared to their peers and TV over time
• Analytical Standards• Custom
• Reference Materials • Proficiency Testing• Compliance Solutions• Laboratory Consultation
Bion Companies and your laboratory
• Process Testing• Fermentation
Applications• Ethanol Testing• Proprietary Analytics
We want to be an extension of your laboratory
