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Monoclonal Antibody Against Nsp2 Proteinof the Highly Pathogenic Porcine Reproductive
and Respiratory Syndrome Virus
Antigen Used for Immunization
Recombinant Nsp2 produced in Escherichia coli.
Method of Immunization
Six to eight-week-old BALB/c mice were immunized threetimes at 2-week intervals with 100mg of the pET28a-Nsp2protein.
Parental Cell Line Used for Fusion
Splenocytes from the BALB/c mice immunized with Nsp2protein were fused with mouse myeloma cells (SP2/0) ac-cording to conventional hybridoma technology.
Selection and Cloning Procedure
Hybridoma cells were grown in HAT selection medium. Po-sitive clones were subcloned by limiting dilution and screenedby ELISA analysis.
Heavy and Light Chains of Immunoglobulin
The MAb belonged to the IgG1subtype, and the light chainwas k type.
Specificity
The specificity was determined by Western blot and IFA an-alyses. The results clearly showed that this MAb could rec-ognize and bind to the Nsp2 specifically.
Specific Antigen Identified
Non-structural protein Nsp2 of the highly pathogenic porcinereproductive and respiratory syndrome virus.
Availability
Tissue culture supernatant Yes X NoAscitic fluid Yes X NoHybridoma cells Yes No X
Reference
Li B, Du L, Sun B, Yu Z, Wen L, Zhang X, Guo R, Ni Y, Hu Y,Zhou J, Zhu H, Lv L, Yu Y, Wang X, and He K: Monoclonalantibodies against Nsp2 protein of the highly pathogenicporcine reproductive and respiratory syndrome virus.Monoclon Antib Immunodiagn Immunother 2013;32(5):362–365.
Address correspondence to:
Dr. Bin LiInstitute of Veterinary MedicineJiangsu Academy of Agricultural Sciences50 Zhong-ling StreetNanjing 210014Jiangsu ProvinceP.R. ChinaE-mail: [email protected]
MONOCLONAL ANTIBODIES IN IMMUNODIAGNOSIS AND IMMUNOTHERAPYVolume 32, Number 5, 2013ª Mary Ann Liebert, Inc.DOI: 10.1089/mab.2013.0048
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