Microalgae Cell Quantification Using Electrical Parameters MICROALGAE CELL QUANTIFICATION USING ELECTRICAL

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  • United Arab Emirates University Scholarworks@UAEU

    Theses Electronic Theses and Dissertations

    5-2016

    Microalgae Cell Quantification Using Electrical Parameters Leena Osama Fahmi Saqer

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    Recommended Citation Fahmi Saqer, Leena Osama, "Microalgae Cell Quantification Using Electrical Parameters" (2016). Theses. 278. https://scholarworks.uaeu.ac.ae/all_theses/278

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  • lmEU (( t )) o� I � jJU I l.:'IIJ La � I Ci.st.o l? '\:J' United Arab Emirates University

    United Arab Emirate Uni ersity

    ollege of Engineering

    Department of lectrical Engineering

    MICROALGAE CELL QUANTIFICATION USING ELECTRICAL PARAMETERS

    Leena Osama Fahmi Saqer

    Thi thesi is submitted in part ia l ful fi l lment of the requirements for the degree of

    Master of Science i n E lectrical Engineering

    U nder the upervi sion of Dr. Mahmoud Al Ahmad

    May 2016

  • Declarat ion of Origin al Work

    L Leena 0 ama Fahmi aqec the under igned, a graduate tudent at the ni ted Arab Emirate l�n i \ ersit) ( EU), and the author o[ th i the is entit led "lUicroalgae Cell QII(/l 1t�f;ca{iol7 C\'ing Electrical Parameter " , hereby. olemnly d c lare that thi

    thesi i' 111) own original r search \\ ork that ha been done and prepared by me under the uper\' i ion of Dr. Mahmoud I hmad, in the o l lege of Engineering at A rhi \\�orh. ha not pre\'iou ly been pre ented or publ i shed, or fomled the basis for

    the a\'\-ard of an a ademic degree, diploma or a imi lar t it le at th is or any other

    univer it) . Any material borrowed from other sources ( whether publ i shed or

    unpllb l i bed) and rel ied upon or inc luded in my the i ha e been properly c i ted and

    ach.n \\ ledged in accordance with appropriate academic convention . I further

    dec lare that there is no potential conflict of interest with respect to the re earch. data

    col lecti n, authorship, pre entat ion and/or publ ication of thi s thesis.

    tlld en l' i gnahu'e: __ ---==,..:.:-----.:::::d::-::::...,==---____ _ Date : _�5"-L/_· �---,-' I_L_o_, Co_

    i i

  • Copyright.£; 20 1 6 Leena Osama Fahmi Sager

    A l l Rights Reserved

    i i i

  • iv

    Appro a] of t he Ma ter The i

    Thi Ma ter The i i approved b) the f, l lowing amining Committ e Member :

    1 ) d\'i or ( Committee hai r ) : Dr Mahmoud I hmad

    Tit le : ciate Profe or

    Department of Ie trical Engin ermg

    liege of Engineering

    ignature �. 2) 1 ember ( Internal Examin r ) : Dr. Falah Awwad

    Title: Associate Profes or Department of E lectrical Engineering

    Coli g of Engineering

    ") 1ember ( External Examiner) : Prof. Mona Zaghoul

    Tit le : Profes or

    Department of E lectrical and Computer Engineering

    Date 2j 6j 2-D I -b

    Institution: The George Washington niversity, USA.

    Signature -r+r-f--+--r�'-b""'::">';�1--- Date Lfjllf I 20 f b I /

  • Thi 1a ter Th accepted b} :

    Dean of the Col lege of Engineering: Professor abah lka s

    ignature _� __ �_�_edz) __ .f" ___ :-=--__ _ Date ---------

    Dean of the Col lege of the raduate tudie : Profe or Nagi T. Wakim

    igna[ure � Date s=, � , 2 I G

    Copy � of�

    v

  • vi

    Ab t ract

    Electrical pr pert ie of l i ving cel l s have b en prov n to playa sign ificant role

    in under tand ing and characterizing the di fferent bi logical act iv i t ies of th cel l . The

    obje t iye of this work. is to devel p an lectri al ba ed technique for d termin ing and

    e t imating the number of m icroalgae cel l i n a u pen ion without the need for any

    ample treatment or pre-proce ing.

    The propo ed techn ique i based on the di rect u e of electrical capacit ive

    model . fhe ba ic premi e beh ind thi id a is the electrical polarizat ion of microalgae

    particles that get charged due to the appl ication of an electric field . Thi wouldn't cau e

    an) di tort ion or d i o lv ing/di ffu ion of the content of the cel l s i nside the medium .

    Th electrical mea urements of the capacitance - voltage concept i s emplo ed to

    determine microalgae cel l count . The microalgae cel l s are considered as dopant

    embedded inside a relevant medium. The cel l s count i s then estimated by subtract ing

    the i ntrin ic impuri ties of the medium from the effecti e ensemble impuri t ies of the

    suspension i nside a defi ned volume. Three strains of m icroalgae. namely

    XOl1nochlorop i . Tetra. and Scenedesmu were cult ivated and examined under the

    proposed methodology. For validation. samples with unknown cel l counts were

    quanti fied u ing the proposed method and compared to other techniques used for

    val idation.

    Results of the study revealed that cel l count determined with the propo ed

    electrical based methodology was done with in few minutes, which is significantly

    shorter than al l other reported techniques. The enumeration of microalgae cel l s count

    is i mportant for their growth optimization. A real t ime. rapid and efficient technique is

  • vii

    needed � r uch purp e. The propo ed method pro"ided a better combination of high

    'en. it i \ it) , quick re pon e, rev. minute . low co t. high throughput. and ea e o[ u e .

    [he outcome f thi \-\- ork al low the development of a rapid technique [or the

    determination of cel l count o[ microalgae or it could be further extended to detemline

    the cel l count o[ other typ of su pended cel l s of comparable size. In addit ion. the

    propo ed methodolog) could be upgraded to b appl ied in-situ with a feedback loop

    that cou ld al low for cont inuous monitoring of the growth conditions and rapid

    detemlination of microalga cel l s count .

    Keyword : Capacitance - Vol tage measurement . cel l count, e lectrical parameter,

    electrical characterization. microalgae.

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