Progressing Biofuel Production from Microalgae … · Progressing Biofuel Production from Microalgae Towards Commercial ... and biofuels, is focused on using ... Marine microalgae

Page 1 © Cellana 2014

Progressing Biofuel Production from Microalgae Towards Commercial Success May 2014

Valerie Harmon, Senior Director R&D© Cellana Inc. 2014

Page 2 © Cellana 2014© Cellana 2014Page 2

Company Overview


Our Mission

Cellana, a developer of algae-based bioproducts

and biofuels, is focused on using the most

productive plants on earth – marine microalgae – to

produce nutritional oils, foods, aquaculture and

other animal feeds, renewable chemicals, and

biofuels, while simultaneously reducing industrial

emissions of CO2.


Cellana Addresses the Necessity for Development of Sustainable Food, Feed, Nutraceuticals, & Fuels

Desire for Sustainable Living

Increasing Human Populations/Growing Demand for Food,

Feeds and High Value Chemicals

Environmental Concerns/Climate Change

Enabling Gov’t Policies:Food Security & Energy


Cellana’s Vision of Microalgae

• Untapped resource!• Efficient harvest of the

sun’s energy• Simultaneous production

of:– Essential nutrients– Essential compounds such

as fuels and valuable chemicals


Microalgae


Kona Demonstration FacilityA commercially viable, sustainable

business strategy in action!


Intensive, Efficient Algae Production at the Kona Demonstration Facility (KDF) on Hawaii

• 2.5 hectare site in Kona, HI

• $20MM replacement cost; owned free & clear by Cellana

• >750,000 liter large-scale cultivation capacity

• Produced over 13 tons of microalgae since 2010

• Commercially significant biomass/oil yields (over 15 g/m2/day productivity)

• 25 full time employees


>100 high-performing strainsScreening criteria mainly emphasize:

Cellana Maintains a Large Private Culture Collection

1. Omega-3 concentration2. Biomass yield3. Amino-acid profile4. Lipid profile5. “Ubiquitous” non-GMO strains


Cellana’s ALDUO™ Algae Production Technology: Proprietary, Photosynthetic, & Proven

Closed SystemPhotobioreactors (PBRs)

Contamination-minimizedMonocultures

(continuous production; inoculates open ponds)

Open SystemOpen Raceway Ponds

Consistent Batch Production (fully harvested 3-7 days after inoculation; re-inoculated at

end of last day with new batch)

Covered by US Patents 7,770,322 & 5,541,056, Similar Patents/Patents pending in Europe, Australia, South Africa, Brazil, Japan, Mexico

+


High

100% Open Ponds

100% PBRs

Risk of Contamination

Low

Cost <50% PBRs / >50% Open Ponds

HighLow

Covered by US Patents 7,770,322 & 5,541,056, Similar Patents/Patents pending in Europe, Australia, South Africa, Brazil, Japan, Mexico

Cellana’s ALDUO™ Algae Production Technology: Cost Effective Commercial Strategy


• Many kinds of algae for many types of products• Cost-effective and reliable generation of quality

inoculum / biomass• Successful large scale cultivation of 11

microalgae strains equating to 7 different species cultivated

Cellana’s ALDUO™ Algae Production Technology: Advantages


Omega-3 nutritional oils, foods, high-value aquaculture / animal feed products and fuels are an extension of Cellana's core competency – producing microalgae.

Cellana’s Biorefinery Business Model:


Cellana’s Biorefinery Business Model:Nothing goes to waste!

We use every component of our

biomass


Commercial-Scale Off-Take Agreement with Neste Oil

• Off-Take Agreement for algae oil announced June 2013

• Neste Oil is the world’s largest refiner of renewable diesel (NExBTL)

• Multi-year off-take agreement• Commercial-scale quantities of algae oil (“up

to tens of thousands of tons”)• Contingencies for Cellana production

capacity, sustainability criteria, and other factors

• Non-Exclusive for both parties• “Samples have shown that Cellana is able to

produce algae oil suitable for renewable fuel production by Neste Oil.”

• “The off-take agreement with Cellana allows us access to commercial-scale volumesof cost-competitive algae oil in the future.”

Neste Oil's renewable fuel plant in Rotterdam in the Netherlands was commissioned in 2011.

Neste Oil's plant in Singapore is the world's largest renewable diesel plant.


Kona Demonstration FacilityLarge-scale Production of Microalgae using Flue Gas


Successful Flue Gas Utilization at KDFSupported by DOE/NAABB


Successful Flue Gas Utilization at KDF

• Experiment run with binary ponds and replicated in time• Flue gas captured from diesel generators that power the site• Flue gas cooling and soot removal accomplished in a storage tank

with water spraying into the tank• Additional soot removal via an air filter• Gas injected into algae culture with a sparger



0

100

200

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400

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700

D1- AM D1- PM D2- AM D2- PM D3- AM D3- PM D4- AM D4- PM

Bio

mas

s, m

g/L

Pure CO2-1Flue gas-1Pure CO2-2Flue gas-2

• No significant difference in N consumption or in growth between ponds grown with pure carbon dioxide vsponds grown with flue gas

0

100

200

300

400

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800

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D1-

AM

D1-

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D2-

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D3-

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D4-

PM

Inor

gani

c N

itro

gen

(µM

) Pure CO2-1

Flue gas-1

Pure CO2-2

Flue gas-2


Heavy Metal Analysis of Biomass Grown on Flue Gas

Heavy metals

Biomassgrown with pure CO2

(ppm)

Biomass grown with

Flue gas (ppm)

IUPAC guideline

(ppm)

Arsenic 1.49 1.55 <5

Cadmium 0.011 0.026 N/A

Lead 0.04 0.096 <2

Mercury <0.01 <0.01 0.1

Biomass grown on flue gas well within IUPAC (International Union of Pure and Applied Chemistry) guidelines for heavy metals



0%10%20%30%40%50%60%70%80%90%100%

0%10%20%30%40%50%60%70%80%90%

100%

Pure CO2-1 Flue gas-1 Pure CO2-2 Flue gas-2

otherAshMoistureCarbsProteinLipidsFAME

0%

5%

10%

15%

20%

25%

30%

35%

C8:0

C12:0

C14:0

C15:0

C16:0

C16:1

C16:2

C18:0

C18:1

C18:2

C18:3

C20:4

C20:5

Fatty acids p

rofile (%

total FA) Pure CO2‐1 Flue gas‐1

Pure CO2‐2 Flue gas‐2

• No significant difference in proximate composition of biomass produced or in lipid profiles


Kona Demonstration FacilityLarge-scale Production of Microalgae

Biomass for Animal Feeds


• Finfish, shellfish, chicken, pigs, cattle – most major sources of meat

• Successful large-scale feed trial for Salmon, Carp, & Shrimp– Marine microalgae from biorefinery as a potential feed protein

source for Atlantic salmon, common carp and whiteleg shrimp, V. Kiron (Bodo University) et al., published online: Aquaculture Nutrition, 3 APR 2012▪ Cellana’s ReNew Feed was acceptable for the three animals at the maximum

levels tested (Salmon 10%, Carp 40%, Shrimp 40%)▪ There were negligible differences in growth and hardly any in the biochemical

composition during the study period

• Successful large-scale feed trial for Broiler Chicks– Potential and Limitation of a New Defatted Diatom Microalgal Biomass in

Replacing Soybean Meal and Corn in Diets for Broiler Chickens, Xin Gen Lei (Cornell) et al., published online: J. Agric. Food Chem., 4 JULY 2013▪ Cellana’s ReNew Feed could substitute for 7.5% of soybean meal alone, or in

combination with corn, in diets for broiler chicks when appropriate amino acids are added

Over 5 MT of Cellana’s ReNew Feed Used in Diverse Feed Trials; All Trials Successful To Date


Atlantic SalmonMarine Microalgae 12 Week Feeding Trial

99% 100% 100% 100% 99%

62% 56%49%

63%51%

0%

20%

40%

60%

80%

100%

120%

Control (CO) C323 (5%, L3) C323 (10%, H3) C88 (5%, L8) C88 (10%, H8)

Salmon Survival (%) Salmon Weight Gain (%)Marine microalgae from biorefinery as a potential feed protein source for Atlantic salmon, common carp and whiteleg shrimpV. Kiron, W. Phromkunthong, M. Huntley, I. Archibald, G. De ScheemakerArticle first published online: Aquaculture Nutrition, 3 APR 2013C323= Nanofrustulum (Bacillariophyceae) C88= Tetraselmis (Chlorophyceae)


Control: CO C323: 5% C323: 10%

C88: 5% C88: 10%

Salmon at the End of the Feeding Trial

“No significant differences in growth or feed performance were observed for algae-based feeds relative to controls, at either the 5% replacement level or at the 10% replacement level.”

Marine microalgae from biorefinery as a potential feed protein source for Atlantic salmon, common carp and whiteleg shrimpV. Kiron, W. Phromkunthong, M. Huntley, I. Archibald, G. De ScheemakerArticle first published online: Aquaculture Nutrition, 3 APR 2013


Common CarpMarine Microalgae 12 Week Feeding Trial

100% 100% 100% 100% 97%

575%533%

577% 582%635%

0%

100%

200%

300%

400%

500%

600%

700%

Control (CO) C323 (25%, L3) C323 (40%, H3) C88 (25%,L8) C88 (40%, H8)

Carp Survival (%) Carp Weight Gain (%)Marine microalgae from biorefinery as a potential feed protein source for Atlantic salmon, common carp and whiteleg shrimpV. Kiron, W. Phromkunthong, M. Huntley, I. Archibald, G. De ScheemakerArticle first published online: Aquaculture Nutrition, 3 APR 2013C323= Nanofrustulum (Bacillariophyceae) C88= Tetraselmis (Chlorophyceae)


Control: CO C323: 25% C323: 40%

C88: 25% C88: 40%

Common CarpMarine Microalgae 12 Week Feeding Trial

“The performance parameters of the groups of commoncarp that received algae-based feeds did not differ significantly from those of fish that were offered the control fishmeal-based feed….”



Whiteleg ShrimpMarine Microalgae 12 Week Feeding Trial

86% 91% 91% 91% 86%

369%398% 385% 374% 387%

0%50%

100%150%200%250%300%350%400%450%

Control (CO) C323 (25%, L3) C323 (40%, H3) C88 (25%, L8) C88 (40%, H8)

Shrimp Survival (%) Shrimp Weight Gain (%)Marine microalgae from biorefinery as a potential feed protein source for Atlantic salmon, common carp and whiteleg shrimpV. Kiron, W. Phromkunthong, M. Huntley, I. Archibald, G. De ScheemakerArticle first published online: Aquaculture Nutrition, 3 APR 2013C323= Nanofrustulum (Bacillariophyceae) C88= Tetraselmis (Chlorophyceae)


Control: CO

C323: 25%

C323: 40%

C88: 25%

C88: 40%

Control: CO

C323: 25%

C323: 40%

C88: 25%

C88: 40%

Whiteleg Shrimp:Successful 12-Week Feeding Trial

“The shrimp growth and feed performance data did not reveal any statistically significant differences during the entire period. However, body protein was lower (P < 0.05) at higher inclusion level of MAP8 compared to those of the control shrimp and L3.”


“Whiteleg shrimp accepted all the test feeds readily, demonstrating the palatability of the new ingredients. Shrimp that were fed algae-based feeds did not differ from the control fishmeal-fed group in terms of their growth and feed performance….”


Conclusions from the Successful Feed Trials

• The algal replacements were acceptable for the three animals at the maximum levels tested– Salmon 10%, Carp 40%, Shrimp 40%

• There were negligible differences in growth and hardly any in the biochemical composition during the study period

• Other feed trials with chickens and pigs equally successful


Summary


Cellana is the Choice to Commercialize Algae-Based Omega-3s and Proteins

• Most Sustainable Feedstock on the Plant– Low carbon footprint

– Low fresh water footprint▪ Especially when compared to fermentation-based products or

terrestrial-crop-based products

▪ Salt- and brackish-water strains with just a rinse step at the end

– Low/no arable land footprint

– Non-GMO algae

• Patented ALDUO™ Production Technology

• Flexible and Economic Algae Biorefinery Model

• Demonstrated Viability of Cellana’s ReNew™ Feed through Trials– Over 6 tons of ReNew™ Feed supplied for trials, 2008-Present

• Demonstrated Viability of Cellana’s ReNew™ Fuel via off-take with Neste

• Validated, Modular, Commercial-Ready Platform– Over $100MM invested in R&D, facilities, strains, IP


Proud Member of ATP3 Consortium


Thank You to the staff of Cellana and the agencies that fund our research For further information please visit

www.cellana.com

or contact:

Valerie HarmonSr. Director of R & [email protected]

Documents

Progressing Biofuel Production from Microalgae … · Progressing Biofuel Production from Microalgae Towards Commercial ... and biofuels, is focused on using ... Marine microalgae