LIVRET D’ACCUEIL - Ecologie Microbienne Lyon · Web viewGENERAL INFORMATIONS and SECURITY INSTRUCTIONS-UMR 5557 La Doua Site Welcome to the Microbial Ecology Unit (UMR 5557). Whether

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- BOOKLET for NEWCOMERS- GENERAL INFORMATIONS and SECURITY INSTRUCTIONS-

UMR 5557 La Doua Site

UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex

Tél 04 72 43 13 77 – Fax 04 26 23 44 68Update : May 2015

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Welcome to the Microbial Ecology Unit (UMR 5557).

Whether you are based in one of four buildings on the Doua site (the fourth and fifth floors of the Mendel building, the first floor of the Lwoff building , the second floor of the Dubois building and the fourth floor of the Forel building), you will find in this booklet a number of relevant information concerning the people who work here, the floor plans, laboratory contact information, rules....In addition, this booklet contains essential points concerning safety in the laboratory.

These instructions concern all newcomers, this form should be read, signed and respected.

PLAN

GENERAL ORGANISATION OF THE LABORATORY p3UPON ARRIVAL TO THE LABORATORY ..... p9GENERAL RULES p10SECURITY IN THE LABORATORY p12RESPONSIBILITIES p13GOOD LABORATORY PRACTICES p15WASTES MANAGEMENT p18RESPECT RULES AND ENSURE RULES ARE RESPECTED p21WHAT TO DO UPON INCIDENT, ACCIDENT P25

SIGNALING NOTE p27SIGNATURE OF NEWCOMERS p28



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GENERAL ORGANISATION OF THE LABORATORY

The UMR CNRS 5557 unit includes over a hundred members, researchers, lecturers, technicians, engineers, and administrative staff, including PhD students, Master 1 & 2 students, and others. These people are spread over several sites: The Doua site, the ENVL and Rockefeller sites, with extremely diverse constraints such as schedules. The UMR 5557 currently includes eight teams (see organigramme below), which are distributed over the sites mentioned above.This booklet of health and safety rules specifically concerns all users of the various buildings on the La Doua site, Université Lyon 1: Mendel, Lwoff, Dubois and Forel buildings.

UMR members who plan to work over the two other sites will need to learn about the rules of operation from the Security responsible agent (APs) of each site.

In all cases, your continued participation to the laboratory life is essential to maintain safety, comfort, harmony as well as good working conditions within the UMR.Be careful to take into account the operating constraints that apply to everyone.Do not hesitate to question the responsible persons for any situation that seems abnormal, whether this concerns you directly or not.

There is only one email address for all your enquiries concerning health and safety aspects and to report any problem (accidents, dangerous situation ...):

[email protected]



- 4 -Distribution of the different buildings housing teams of the Ecologie Microbienne Laboratory on the La Doua site

UMR 5557 – Université Lyon 1 – Site La Doua -16-10-6 rue Raphaël Dubois – 69622 Villeurbanne Cedex Tél 04 72 43 13 77 – Fax 04 26 23 44 68

Update : May 2015

Meeting

- Library

413

4°C room

37°C room

418

418ICommon Room:

Equipment +BET area

420 422

Washing room

421

Team 5PGE+Team 3

PCR room Laminar flow hood

419417415

MENDEL 4 th floor - SOUTH

Chromatography

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Plans of the Mendel building floors



Radioactivity Room

22

Small meeting room

406

405 407 413

Team7

402 P2

403

406 410 412 414

Team 4

416

Team 1

MENDEL 4 th floor - NORTH

Team 5

Printers - Fax -Photocopying machines

Team 1iBio

- 6 -Building plan Mendel (5th floor – North)

(Incubating room, Microscopy room, Printers, Freezers room, Laminar flow hoods, Common machines room)

Building plan Mendel (5th floor – South)



- 7 -Lwoff and Forel buildings plans

Lwoff 1st Floor - 10 rue Raphael Dubois.

Lwoff Ground floor - 10 rue Raphael Dubois.



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FOREL 4th Floor- 6 rue Raphael Dubois.

DUBOIS 2nd Floor- 6 rue Raphael Dubois. Administration



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YOU HAVE JUST ARRIVED IN THE LABORATORY ..... Your tutor is directly responsible for your installation, your safety and your good integration in the laboratory, and with him you must:

• Make sure you have completed all formalities with the University and / or the CNRS and you have obtained the necessary safeguards for your activity in the laboratory, especially in case of accident (liability insurance, training agreement, etc ...),

• Get to know your colleagues, and give your name to the laboratory secretariat (Christine Delorme Tel 04.72.43.13.77 Forel Bldg)

• See Danis Abrouk to obtain an access badge to the laboratory,

• Contact the person responsible for health and safety, called ACMO in your building:Mendel Building Audrey DUBOST, AP Référent [email protected] Building Jeanne DORE, AP [email protected]

Florence-Hélène TRAN, AP [email protected] Building Guillaume MEIFFREN, AP [email protected]

Laurence LOISEAU, AP [email protected]

Rockefeller Site Delphine MOUNIEE, AP [email protected] MICHALET, AP [email protected]

Vetagro sup Site Marion BOUVIER CROZIER, correspondant H&S

[email protected]

HEALTH AND SAFETY AT WORK is it available at the entrance to each floor

• Make contact, through the person who tutors you, with the person competent in radiation protection, Bruno TILLY [email protected] if you need to handle radioisotopes.The list of documents you should receive comprises:

• Badge of the laborator• Information sheet• Internal rules• Material Safety Data Sheet• Safety Booklet .

INTERNAL RULES

In any event, all staff must work 7h42min daily for 38.5 hours weekly. Working time is "effective" working time. It does not comprise the mandatory lunch break that cannot be less than 45 minutes nor more than two hours.

HOWEVER ALL MEMBERS OF THE LABORATORY MUST BE PRESENT AND IN ACTIVITY OVER AN INCOMPRESSIBLE TIME PERIOD RANGING FROM 9:30 TO 11:30 AND FROM 14H TO 16H.

Attendance to the weekly laboratory meeting is compulsory. The meeting is held on tuesdays at 12h45, lasts for about an hour and permit transmission of information to all.

Working "alone" and working outside 'normal' hours

• It is important to know that working «alone " has no clear legal framework to date. It should remain exceptional and punctual. Should any mishap occur, the criminal responsibility of the Unit Director Unit and that of the concerned personnel is engaged.



mailto:[email protected]








- 10 -• For reasons of safety and liability, the laboratory director must be informed of

persons likely to come to work outside normal hours including during weekends, and he must have agreed to it.

• It is important to realize that working "alone" even if it does not cause additional risk by itself, greatly increases the time of implementation of the alert in case of emergency incident / accident.

• To date authorized personnel are:o researchers and teacher-researchers and PhD students for research purposes,o Technical personnel (ITA / IATOS) for clearly identified service-related reasons, but

never alone. All other agents who do not have a service reason should not come to the lab after hours.

• In particular, trainees (Master 1 or 2, ATER, BTS, etc.) should not be alone in the laboratory in the absence of their tutor or of an authorized person. It is the responsibility of individual tutors to ensure compliance with this rule.

Missions

All staff traveling in France or abroad in the framework of his functions for Meetings, Collaborations, Courses or Training must be in possession of a signed mission order which is thus a legally compulsory requirement. This document is mandatory in terms of administrative and legal requirements, it provides insurance coverage of the agent in case of Accidents occuring during the Service. In the absence of a signed mission order, there is no insurance coverage.

• In addition, any agent required to go directly from home to a temporary place of work without going first to his normal administrative place of work, is covered in case of accident, subject to fulfilling one of the following two conditions:- Be in possession of a signed mission order- Have a written statement by the laboratory director.

Contact:[email protected]@listes.univ-lyon1.fr





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SECURITY IN THE LABORATORYYou are in a research laboratory that is also designed to accommodate students receiving teachings given at the University Lyon 1. However, if the rules above apply specifically to research, some issues relate directly to teaching activities, especially in terms of overall safety in the laboratory. The internal regulations of the UMR unit are additions to the specific regulations each agent must respect in relation to his status and his parent organisation (CNRS, University, INRA, ..)

• Respect entrance and exit rules

• Do not leave your belongings unattended: bags, wallets or other.

• Ensure that laptops are secured to prevent theft in offices.

• Do not hesitate to inquire as to the whereabouts of any person you do not know.

• Unless an unusual situation arises, do not lend your laboratory badge to an outsider, please refer to your direct tutor.

• The presence of any visitor (professional or otherwise) that you take the lab is your direct responsibility. Make sure that his presence and activities within the laboratory are consistent with the general running, and do not pose a security problem (authorization to the visitor for work; presence of children in the laboratory, etc..,. ...).



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RESPONSIBILITIESMendel building

Forel BuildingLwoff Building

Labo 123 MEIFFREN Guillaume

Evaporation room

MEIFFREN Guillaume

Open space MEIFFREN Guillaume

GPC-MS Room MEIFFREN Guillaume

LC-MS Room MEIFFREN Guillaume

Chemical wastes

MEIFFREN Guillaume

Biological wastes LOISEAU Laurence

PARMIC Room LOISEAU Laurence



Room 401 (Radioactivity) TILLY Bruno (PCR)

Room 402 CHAPULLIOT David / VIAL Ludovic

Room 403 MULLER Daniel / VACHERON Jordan

Room 404 (P2) BERTOLLA Franck

Room 405 (Microscopes) PUJIC Petar

Room 407 LERONDELLE Catherine

Room 409 bis ABROUK Danis

Room 413 FOURNIER Pascale

Room 416 FOURNIER Pascale

Room 419 PUJIC Petar

Room 420 SANNAIRE Corinne

Room 421 GUILLAUMAUD Nadine

Room 502 WISNIEWSKI Florence

Room 525 BROTHIER Elisabeth

Room 504 En attente de responsable

Chemical and biological wastes DUBOST Audrey

Chemical Products HAURAT Jacqueline / BROTHIER Elisabeth

Molecular Biology Kits HAURAT Jacqueline

Consummables and small supplies FOURNIER Pascale

Centrifuges and rotors PUJIC Pierre

Incubators / pH-meter à discuter

Autoclave/glassware washing machine CHAPULLIOT David

Speed Vac and its pump PRIGENT-COMBARET Claire

Proteomics PUJIC Petar

Agitation apparatus WISNIEWSKI-DYE Florence

101/103 Room TRAN Florence-Hélène

Media preparation Room FRAISSINET-TRACHET Laurence

Autoclave Room DORE Jeanne

Room 111 VALLON Laurent

Room 113 FRAISSINET-TRACHET Laurence

Room 117 LUIS Patricia

Room 122 LUIS Patricia

Chemical wastes DORE Jeanne

Biological wastes DORE Jeanne

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GOOD LABORATORY PRACTICESCommon Protections

Common protection equipments must be used in priority. The main ones are :• fume hoods, ventilated enclosures with air depression, connected by an extractor to the outside.• portable chemical hoods, which suck in air and eject it back into the laboratory after passage

through an activated carbon filter (usually for specific volatile compounds),• protective barriers, which must be resistant and placed before each manipulation of chemicals

that have a projection or explosion hazard• the laminar flow hoods or Microbiological Safety Cabinets (MSC),• in the case of highly toxic products, weighing shall be done under the powder hood.

Individual Protections

Wearing personal protective equipment (PPE) is MANDATORY. This comprises at least:

• A cotton lab coat with long sleeves (no lab coat in offices & out of the lab),

• safety glasses with side shields,

• gloves resistant to the products handled.

Respiratory protection is, if any, provided by masks or cartridge filters adapted to the pollutants one is working with. The masks can be used in autonomous ad hoc interventions. Dust masks do not protect from chemical vapors.

TYPES OF RISKChemical risks

TraductionAll chemicals bottles have label on which are written the risks associated with this product. They generate risks by chemical toxicity, flammability, corrosivity, ...• Learn about the steps to take and the risks associated with product use,• Store used products in their original location.

Biological risks BE UP TO DATE IN YOUR VACCINATIONS !

The laboratory generates biohazards, or more precisely the risks of contamination and infection. The risk is mainly microbial.

Air is the main path of entry for biological agents, it is also the most insidious. They get in by inhaled aerosols or by accidental ingestion. Aerosols generated by centrifuges not closed or opened prior to a complete stop, or unlocked vortex tubes, are poorly quantified and their virulence is difficult to establish. Failure to comply with hygiene rules are unfortunately often sufficient to explain a number of infections : eating in the laboratory (a dining room is available), smoking (all public building are NON-SMOKING), touching your mouth with your hands without previous washing as many mistakes to avoid.



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The mucocutaneous path has three modalities:• broken skin (cut or accidental puncture, projections on broken skin),• projection on mucous membranes,• projection on healthy skin.

Entry through the digestive tract is always due to a breakdown in personal hygiene measures (hand washing default) or to technical errors (mouth pipetting).

Radioactivity Radioactivity should be handled in room 401, that has been set aside for this purpose. When using

radioactivity, contact in advance the manager: Bruno TILLY (04 78 87 25 55 or 06 63 91 27 22 /Mendel Bldg 5th floor and VetAgroSup / [email protected]) .

L2 (type 2 confinement laboratory) Agent in charge: Franck BERTOLLA Any student planning to work in the L2 will: be trained in advance to abide by safety rules (displayed at the entrance) always be under the responsibility of people allowed into the L2 (list at the entrance of L2).

I2 (type 2 confinement insectarium) Agent in charge: Claire VALIENTE MORO and Florence-Hélène TRAN

Any student planning to work in the I2 will: be trained in advance to abide by safety rules (displayed at the entrance) always be under the responsibility of people allowed into the I2.

Equipments under pressure

Pressure equipments are commonly used in our institution. They are used either for research or are linked to buildings safety. Lack of maintenance or improper handling can lead to an explosion, implosion or breakage of fragile items (seals, windows, regulators) and may project shards at high velocity, cause shock waves, leakage of liquid or gas. Apart from the risks related to pressure, some devices have risks that depend on the nature of the product rejected: intoxication, inflammation, explosions.

These are==> Pressure vessels• steam (autoclave for sterilization, boiler, ...)• Gas (autoclave reaction, gas cylinder, compressor and enclosure ...)• liquid==> Devices under vacuum (evaporator or lyophilizer chamber experience, ...)For obvious security reasons, only authorized persons can use autoclaves, these are :




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WASTES MANAGEMENTChemical wastes

Liquid wastes

5L or 10L clear containers (only the label change)

Solid wastes

Syringes,pipettes …

Solid wastes

5, 20 or 30l Pails tips, chemically soiled Eppendorf tubes

Neofuts 70l or 35l disposable barrelsSoiled solid wastes

Fûts à ouverture totale 30 or 60l (Completely open barrels ) Glassware broken or chemically soiled

Identify containers : producing laboratory, product or type of product. Do not mix products : compatibility. Stock containers in a well ventilated room, keep away from heat, sparks or open fire.


Update : May 2015

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Biological wastes

Barrels 30/60l Autorised = All types of biological wastes. Treatment = Incineration at very high temperature.

Cartons 23/50l Autorised = Culture media, Petri dishes, gloves, sharp consumables, conditionned in normalised packing. Treatment = Incineration or standard.

Identify the service of origin on the container. Respect the filling and weight limits of containers. All sharp objects must be conditionned in normalised packing if disposal is done in cartons. Do not store chemical wastes. Store containers in dry room with constant temperature : in the biological waste hold. Store only biological wastes in the biological waste hold.

Objects should be sorted on the lab bench by the experimenter prior to disposal.


Update : May 2015

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Models of labels

Chemical wastes

Biological wastes


Update : May 2015

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RESPECT RULES AND ENSURE RULES ARE RESPECTED. • Ensure that your installation and bench working conditions are known to the people likely to interact with you and that they do not disrupt existing activities.

• Organize, store and clean your bench with ethanol (70 °) after handling.

• Ensure the smooth running of the laboratory in particular at the coffee room and library.

• Allow a smooth working of the air-conditioned rooms, by closing the doors.

• Observe the prohibition of eating, drinking, smoking in the laboratory, a room is available for this.

• Wash your hands after handling.

• Learn to identify different risks from the labels : radioactive, chemical, biological, electrical, etc. risks......

• Learn how to sort and dispose of your wastes. Identify hazardous wastes.

• Learn to use the ventilation devices (fume hoods, chemical hoods)

• Make sure before you bring a visitor or non-professional in the lab that you have approval from your director. Do not let anyone outside the laboratory experiment without the agreement of your tutor. Visitors must be accompanied in experimental rooms.

• When planning a new experiment, consider prevention- Evaluate the risks- Provide the protection means- Plan for waste disposal

• Think about your colleagues and staff to service, maintain your own bench and the equipment you use (balance, centrifuge, ...).

• Because of the danger of explosion, it is forbidden to store flammable solvents in refrigerators (ether, chloroform, etc..).

• As soon as fire doors close or in case of a serious incident,Call « 30 » immediately from any telephone



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Update : May 2015

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Update : May 2015

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Update : May 2015

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WHAT TO DO UPON AN INCIDENT, AN ACCIDENTAny person who witnesses an accident should call for help and indicate:• The nature of the accident,• The number of injured persons and their condition,• The location of the accident,• Additional risks,• The name and telephone number where they can be reached.If the person is a certified rescuer, provide first aid.The person has to go to the entrance of the facility to host and conduct rescuers until the accident.The Laboratory Director, the Head of Service and the ACMO will be notified.Any incident should be entered in the health and safety register.Injuries, even minor, must be registered with the medical service.All accidents must be reported within 48 hours.

TO DECLARE A WORK ACCIDENT In case of a serious incident or accident requiring a statement, you must inform the secretariat of the laboratory- Yvan MOENNE- LOCCOZ or Audrey DUBOST as soon as possible, and file in duplicate documents to the secretariat.Instructions for making an accident report are clearly detailed in the links below, for the different types of staff (University, Students, CNRS):

For the UCB Lyon 1 Universityhttp://www.univ-lyon1.fr/05124120/0/fiche___pagelibre/&RH=INT_MT-URG For the CNRShttp://www.dr7.cnrs.fr/rubrique.php3?id_rubrique=256

Some useful numbers

® Head of the Hygiene and Security Service UCB Lyon 1 Murielle PIERRE 04 72 43 18 79 [email protected]

® Prevention and Security CNRS agentPatricia LANDAIS 04 72 44 56 07 [email protected]

® Work Medecine Personnel Service UCB Lyon 1 Dr GONNAZ 04 72 44 82 11 ou 04 72 43 12 01 (Paul Dirac

bldg)CNRS Dr MICHEL or Dr DUFOURT 04 72 44 56 81

® InfirmerieUCB Lyon 1 Patricia DUCERF 04 72 43 12 02

Isabelle SABOT



http://intranet.univ-lyon1.fr/isabelle.sabot/0/fiche___annuaireksup/


http://www.dr7.cnrs.fr/rubrique.php3?id_rubrique=256

http://www.univ-lyon1.fr/05124120/0/fiche___pagelibre/&RH=INT_MT-URG

- 24 -Marie VANCON-MENEZ 04 72 44 82 55Great amphithaeater hall (La Doua) 04 72 43 16 22

[from monday to thursday (8–16 h), friday (8–11h45)]

CNRS Claire-Françoise VERRIES 04 72 44 56 03

® Inter University Service of Preventive Medecine 04 72 44 40 80 [from monday to thursday (8-12 h and 13-17 h), friday (8-11 h)] - Fax 04.72.44.40.90 - mail : [email protected]




http://intranet.univ-lyon1.fr/marie.menez/0/fiche___annuaireksup/

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SIGNALING NOTE

Name :

Study course :

Team :

Tutor :

Theme :

Dates of stay :


Update : May 2015

photo

Documents

LIVRET D’ACCUEIL - Ecologie Microbienne Lyon · Web viewGENERAL INFORMATIONS and SECURITY INSTRUCTIONS-UMR 5557 La Doua Site Welcome to the Microbial Ecology Unit (UMR 5557). Whether