Innova GOLD Nanoparticles, Conjugation kits and Conjugates › ft › B › BB100i.pdf · 2017-02-16 · Innova GOLD Nanoparticles, Conjugation kits and Conjugates Colloidal gold

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Innova GOLD Nanoparticles, Conjugation kits and Conjugates

Colloidal gold

InnovaCoat® GOLD nanoparticles are developed using specialized techniques

that enable the production of extremely uniform spherical particles with a very

narrow size distribution, minimizing variability within your assay. They have a

proprietary surface coating which covalently binds the antibody or protein

forming highly stable conjugates.

The coated nanoparticles are available within our range of easy-to-use

conjugation kits or separately as carboxylated gold nanoparticles.

Gold nanoparticles conjugation kits

InnovaCoat® GOLD conjugation kit is a one-step procedure to covalently graft

gold nano-particles with antibodies, proteins and peptides or any other

biomolecule with an amine group. Sylfhydryl reactive (Maleimide activated) and

Clickable (Hydrazide activated) kits are available as well.

InnovaCoat® GOLD Conjugates

Our pre-conjugated gold nanoparticles are manufactured using our

InnovaCoat® GOLD nanoparticles which have a proprietary surface coat

that covalently bind the detection protein providing highly stable

conjugates.

Purification kits compatible with InnovaCoat® GOLD Nanoparticles

& Conjugation Kits

Commercially available antibodies often contain substances (e.g. BSA,

glycine, tris, and azide) that interfere in labeling reactions. We have

developed a range of purification kits which allow for easy and rapid

purification of antibodies from any buffer formulation. All products are

easy to use and are fully compatible with our nanoparticle kits. See PH

https://innovabiosciences.com/InnovaGOLD-gold-nanoparticles.html

https://innovabiosciences.com/gold-nanoparticle-conjugates.html

https://innovabiosciences.com/gold-nanoparticle-conjugates.html

https://innovabiosciences.com/abpure-antibody-purification-kits-compatible-with-nanoparticles.html

InnovaCoat® GOLD conjugation kits

InnovaCoat® GOLD nanoparticle conjugation kits enable the easy conjugation of gold nanoparticles to antibodies or

proteins for use in R&D applications and for the development and manufacture of diagnostic kits.

features and benefits

Features Benefits

Quick and easy to use Save time, no special knowledge required

Different nanoparticle sizes, concentrations and

chemistries available Flexible

Freeze dried Ships at ambient temperature, long shelf-life

Fully scalable Easy transfer from R&D to manufacturing

Stringently QC tested Consistent high quality, excellent batch-to-

batch reproducibility

Proprietary surface coating Covalent bond, stable conjugates formed

Covalent conjugation uses significantly less antibody

than passive conjugation Save precious antibody

Uniform spherical shape and narrow size distribution Batch-to-batch consistency

The kits are designed for optimization purposes, for example, for the screening of potential antibodies or small scale

'proof of principle' studies. Please contact us if you require any additional information or to discuss your bulk or

custom conjugation requirements.

Standard Vs. Orientated Labeling

The InnovaCoat® GOLD conjugation kits facilitate standard, orientated, or site specific labeling (sites which do not

include the binding domain) of IgG antibodies.

See more details:

InnovaCoat® GOLD - Gold Conjugation Kits (20OD 10nm-20nm-40nm-60nm-80nm)

InnovaCoat® GOLD - Maleimide Gold Conjugation Kits (20OD 20nm-40nm)

InnovaCoat® GOLD - Hydrazide Gold Conjugation Kits (10OD 40nm)

Please do not hesitate to contact us to find out more!

mailto:[email protected]


Detailed information

InnovaCoat® GOLD conjugation Kits

InnovaCoat® GOLD conjugation kits enable the covalent attachment of antibodies, proteins, peptides and other

biomolecules to gold nanoparticles with a proprietary InnovaCoat® coating*. The hands-on time is around 30 seconds

and the gold conjugate is ready to use after 20 minutes.

List of kits Presentation (benefits & features) Data FAQ

InnovaCoat® GOLD - AMINE target Gold Conjugation Kits See description Tech sheet

• 20OD 10nm

3 Reaction Mini Kit UVA750-228-0005


1 Reaction Midi Kit UVA752-228-0015

• 20OD 20nm

3 reaction Mini Kit 1D5330-229-0005

10 Reaction Mini Kit 1D5331-229-0010

1 Reaction Midi Kit 1D5332-229-0015

• 20OD 40nm

10 reaction optimisation kit JV2790-230-0005


Validation Kit JV2792-230-0015

• 20OD 60nm

3 reactions Mini Kit PQK580-232-0005


1 reaction Midi Kit PQK582-232-0015

• 20OD 80nm

3 Reaction Mini Kit PQK590-231-0005


1 Reaction Midi Kit PQK592-231-0015

InnovaCoat® GOLD – MALEIMIDE Gold Conjugation Kits See description Tech sheet

• 20OD 20nm

3 Reaction Mini Kit YRT700-271-0005

1 Reaction Midi Kit YRT701-271-0015

• 20OD 40nm



InnovaCoat® GOLD - HYDRAZIDE Gold Conjugation Kits See description Tech sheet

• 10OD 40nm

1 reaction optimisation kit PQK650-280-0100


Validation Kit UVA801-280-0300

*InnovaCoat® is a special surface coating material for gold nanoparticles which remains firmly anchored even under the most extreme

conditions. InnovaCoat® GOLD derivatives display unrivalled colloidal stability.

http://www.interchim.fr/ft/P/PQK580.pdf



InnovaCoat® GOLD conjugation kits (Amine reactive)

InnovaCoat® GOLD conjugation kits enable the covalent attachment of antibodies, proteins, peptides and other

biomolecules to gold nanoparticles with a proprietary InnovaCoat® coating. The hands-on time is around 30 seconds

and the gold conjugate is ready to use after 20 minutes.

InnovaCoat® GOLD conjugation kits features and benefits

Features Benefits

Quick and easy to use Save time, no special knowledge required

Different nanoparticle sizes, concentrations and

chemistries available Flexible





Proprietary surface coating Covalent bond, stable conjugates formed

Covalent conjugation uses significantly less antibody

than passive conjugation Save precious antibody

Uniform spherical shape and narrow size distribution Batch-to-batch consistency

To order

InnovaCoat® GOLD - AMINE target Gold Conjugation Kits See description Tech sheet

• 20OD 10nm



1 Reaction Midi Kit UVA752-228-0015

• 20OD 20nm

3 reaction Mini Kit 1D5330-229-0005

10 Reaction Mini Kit 1D5331-229-0010

1 Reaction Midi Kit 1D5332-229-0015

• 20OD 40nm



Validation Kit JV2792-230-0015

• 20OD 60nm



1 reaction Midi Kit PQK582-232-0015

• 20OD 80nm





Data

Click images to view full screen:

Figure 1.

A comparison between the percentage binding of anti-cortisol

antibodies conjugated to 40nm gold particles by a traditional

passive method against those conjugated covalently using

InnovaCoat® technology. All components of this competitive

lateral flow assay are identical with the exception of the method

used to conjugate the antibodies to gold colloid. External data

source.

Figure 2.

A comparison between the signal intensity, measured on a

commercially available lateral flow strip reader, for anti-IgA






source.

Figure 3.

A comparison between the percentage binding of anti-IgA






source.

FAQ

What can I do if my antibody preparation contains interfering substances?

If your antibody buffer is not compatible with our kits, we have developed the AbPure antibody purification

kit range that allows you to quickly and simply purify your antibody and is fully compatible with

InnovaCoat® GOLD.

Please consult the kit protocols to see the antibody amount/volume suitable for each kit.

Does the antibody bind to the metal surface?

No. The protective surface coat completely shields the metal surface and prevents direct metal-antibody

interactions. For this reason, you cannot use InnovaCoat® GOLD for passive conjugation of antibodies.

Can antibodies from different species be used?

Yes, the InnovaCoat system has been tested with antibodies from a variety of species including mouse,

rabbit, goat and sheep.

What type of linkage to gold is formed?

The antibody becomes covalently and irreversibly attached via lysine residues to the InnovaCoat® surface.

https://innovabiosciences.com/images/stories/innova/images/cortisol_annotated_web.jpg

https://innovabiosciences.com/images/stories/innova/images/cortisol_annotated_web.jpg

https://innovabiosciences.com/images/stories/innova/images/iga_sig_annotated_web.jpg

https://innovabiosciences.com/images/stories/innova/images/iga_sig_annotated_web.jpg

https://innovabiosciences.com/images/stories/innova/images/iga_bin_annotated_web.jpg

https://innovabiosciences.com/images/stories/innova/images/iga_bin_annotated_web.jpg

Can antibody fragments be conjugated?

Yes. One of the advantages of the protective coat is that it is less likely than a bare metal surface to cause

denaturation and loss of affinity of the antibody fragments.

What if I need bulk material?

The kit that you have purchased is a convenience product for rapid production of small quantities of

conjugate for screening purposes. Bulk quantities of InnovaCoat GOLD can be provided in a variety of

formats. Please enquire.

Is the kit suitable for conjugating analytes and other small molecules?

Yes, but please contact Innova technical services. Depending on the functional groups on your analyte you

may need a different type of surface or different chemistry for optimal results.

InnovaCoat® GOLD - Maleimide Site Specific Labeling Kit (Sulfhydryl reactive)

The InnovaCoat® GOLD-Maleimide conjugation kit allows thiolated molecules (e.g. antibodies, Fab'

fragments, or other sulfhydryl-containing biomolecules) to be covalently attached to ultra-stable

InnovaCoat® GOLD nanoparticles at very high OD quickly and easily.

The researcher simply has to pipette the solution of the thiol-activated molecule to the vial of InnovaCoat

GOLD maleimide. Thee hands-on time for the procedure is about 5 minutes and the conjugate is ready to use

within 1 hour.

Performance Enhancement by Orientated Coupling

The InnovaCoat® protective

coating completely shields

the biomolecule from the

metal surface thus

eliminating metal-antibody

interactions and reducing the

risk of sub-optimal antibody

performance (e.g. loss of

affinity or denaturation,

especially of small antibody

fragments). Moreover, the

covalently attached molecule

is not prone to dissociation

which is a major problem

with direct thiol-to-gold

metal conjugations.

InnovaCoat® GOLD-Maleimide nanoparticles in this kit are supplied as a freeze dried mixture. The

conjugation reaction is initiated simply by adding a solution of the thiolated biomolecule, which becomes

attached to the InnovaCoat® surface by a covalent reaction with maleimide groups.

InnovaCoat® GOLD-Maleimide Features and Benefits

Features Benefits

Oriented labeling Oligonucleotides, and the antigen binding sites of

antibodies are free to bind the target molecule

Proprietary surface coating prevents metal-

protein interactions Covalent bond, stable conjugates formed

Different nanoparticle sizes and amounts

available Flexible



Stringently QC tested: uniform spherical

shape and narrow size distribution

Consistent high quality, excellent batch-to-batch

reproducibility

InnovaCoat® GOLD-Maleimide Site Specific Labeling Kit can be provided in a variety of pack sizes.

To order

InnovaCoat® GOLD – MALEIMIDE Gold Conjugation Kits See description Tech sheet

• 20OD 20nm

3 Reaction Mini Kit YRT700-271-0005

1 Reaction Midi Kit YRT701-271-0015

• 20OD 40nm



Data

Lateral flow assay demonstrates increased

performance of orientated (Fab'-InnovaCoat®

GOLD-Maleimide) vs. random (Fab-

InnovaCoat® GOLD) labeling. All components

of the lateral flow assay are identical with the

exception of the method used to conjugate the

Fab' to InnovaCoat® GOLD-Maleimide or Fab

to InnovaCoat® GOLD. Fab and Fab'

fragments were derived from the same

antibody.


InnovaCoat® GOLD - Hydrazide Site Specific Labeling Kit (Click, aldehyde reactive)

Ultra stable InnovaCoat® GOLD particles functionalized with hydrazide groups permit the quick and easy attachment

of antibodies. The kit allows to gently oxidize the sugars on the Fc region of the antibodies and convert them to

aldehydes. The aldehydes react with the hydrazide groups on the coated gold to form stable hydrazone bonds. The two

antigen binding arms are therefore orientated in space, which may lead to enhanced assay performance (please refer to

the diagram below).

Performance Enhancement by Orientated Coupling

InnovaCoat® GOLD-Hydrazide features and benefits

Features Benefits

Site-specific labeling Antigen binding sites of antibodies are free to

bind the target molecule

Proprietary surface coating prevents metal-protein

interactions Covalent bond, stable conjugates formed


Stringently QC tested: uniform spherical shape

and narrow size distribution

Consistent high quality, excellent batch-to-batch

reproducibility

InnovaCoat® GOLD-Hydrazide Site Specific Labeling Kit can be provided in a variety of pack sizes.

To order

InnovaCoat® GOLD - HYDRAZIDE Gold Conjugation Kits See description Tech sheet

• 10OD 40nm



Validation Kit UVA801-280-0300

Data

Lateral flow assay demonstrates increased

performance of site-specific (IgG-

InnovaCoat® GOLD-Hydrazide) vs.

random (IgG-InnovaCoat® GOLD)

labeling. All components of the lateral flow

assay are identical with the exception of the

method used to conjugate the oxidised IgG

to InnovaCoat® GOLD-Hydrazide or IgG

to InnovaCoat® GOLD. Oxidised IgG and

IgG were derived from the same antibody.


Innova GOLD nanoparticles

InnovaCoat® GOLD nanoparticles are coated gold nanoparticles that have a proprietary surface coating.

•The coating material remains firmly anchored even under the most extreme conditions. InnovaCoat®

GOLD derivatives display unrivalled colloidal stability.

•These nanoparticles are available within our range of easy-to-use conjugation kits or separately as

carboxylated gold nanoparticles (InnovaCoat® GOLD-Carboxyl nanoparticles).

The GOLD nanoparticles are functionalized with carboxyl groups and allow the covalent conjugation of

antibodies, or other proteins, using the water soluble carbodiimide crosslinker EDC. They form highly stable

conjugates. Standard chemistry can make conjugation on other functional groups, such as sulfhydryls or

aldehyde and Click-partners (acetylene, DNCA, HyNic).

To order InnovaCoat® GOLD – Carboxyl gold nanoparticles

10nm gold nanoparticles

1R6990 1 OD 20ml 3001-0020 1 OD 100ml 3001-0100

1R7000 10 OD 20ml 3010-0020 10 OD 100ml 3010-0100


A1LZO0 1 OD 10ml 3201-0010 1 OD 100ml 3201-0100

A1LZP0 10 OD 10ml 3210-0010 10 OD 100ml 3210-0100

A1LZQ0 15 OD 10ml 3215-0010 15 OD 100ml 3215-0100


UVA722 1 OD 10ml 201-0010 1 OD 100ml 201-0100

UVA732 10 OD 10ml 210-0010 10 OD 100ml 210-0100

0A3920 15 OD 10ml 212-0010 15 OD 100ml 212-0100

UVA742 20 OD 10ml 220-0010 20 OD 100ml 220-0100

40OD Carboxyl 0B5680 40 OD 1ml 3400-0001 40 OD 5ml 3400-0005


A1LZV0 1 OD 10ml 3801-0010 1 OD 100ml 3801-0100

A1LZW0 10 OD 10ml 3810-0010 10 OD 100ml 3810-0100

A1LZX0 15 OD 10ml 3815-0010 15 OD 100ml 3815-0100

For large quantities of our colloidal gold please contact us and we will send you more information.

Colloidal gold applications

Application Particules Size Shape

10nm 20nm 40nm 80nm

Lateral Flow ● ● ● Sphere

Western Blots ● ● ● Sphere

Dot Blots ● Sphere

Dark Field microscopy ● Sphere

DLS ● ● ● ● Sphere

Flow Cytometry ● ● Sphere

Electron microscopy ● ● Sphere


https://www.innovabiosciences.com/applications/dot-blot.html

https://www.innovabiosciences.com/applications/flow-cytometry.html

Metal enhanced fluorescence ● Sphere/flat surface

SERS ● ● Not spherical

FRET quench ● ● ● Sphere

Infrared phototermal therapy ● ● Rod/hollow sphere

Radiofrequency ablation ● Sphere

Drug delivery ● ● ● Rod/sphere

Carboxylated gold nanoparticles 40OD 40nm

Composition: 40nm carboxyl-gold 40 OD

Size: 40nm

Adsorption max: 530nm

OD: 40.0

Particles per ml: 36 x 1011

Volume: 1ml and 5ml

Buffer: 10mM EPPS pH 8.5

InnovaCoat® GOLD-Carboxyl nanoparticles are coated gold nanoparticles functionalized with carboxyl groups that

allow the covalent conjugation of antibodies, using the water soluble carbodiimide crosslinker EDC. Unlike traditional

EDC/NHS coupling used to activate carboxyl groups, InnovaCoat® GOLD-Carboxyl nanoparticles are optimized for

single step EDC covalent coupling without signs of aggregation. This eliminates the need for EDC/NHS pre-

activation and washing steps associated with traditional conjugations.

This process is also significantly faster than standard methods, with conjugates ready to use in less than 35 minutes.

The product enables the generation of highly stable covalent gold-antibody conjugates at R&D and manufacturing

scales.

InnovaCoat® GOLD carboxyl nanoparticles have a narrow size distribution, a uniform spherical shape and high batch-

to-batch consistency.

Mean Diameter SD CV%

36-44nm <4nm <10%


Our 10nm colloidal gold is an aqueous suspension of spherical metallic

nanoparticles manufactured to the highest standard to ensure:

Uniform spherical shape

Narrow size distribution: 10nm ± 1nm, <10% CV

Ultra high quality

Batch-to-batch consistency

High efficiency antibody binding for maximum sensitivity

Available at high concentrations to simplify assay development

Narrow size distribution and consistent shape is important and

preferable for most applications.

A consistent, spherical shape means that particles

are less prone to aggregation which can be a

problem associated with gold nanoparticles.

Furthermore uniform shape ensures consistent

binding of antibodies, ensuring reliable assay results.

Our 10nm gold nanoparticles have a narrow size

distribution (Figure 1.), with a CV (standard

deviation/diameter) of less than 10%.

Each batch of 10nm colloidal gold undergoes TEM

analysis to ensure all the requirements are met.

Our colloidal gold nanoparticles are suspended in citrate

buffer and they are suitable for:

Protein absorption

Oligonucleotide absorption

Surface modifications

Specifications - Size distribution and shape

Figure 1. Size distribution of the 10nm gold nanoparticles

displaying a narrow size distribution with a CV (standard


Mean diameter SD CV% %Irregular Shapes

9-11nm <1nm <10% <10%

We can also ensure our gold nanoparticles have less

than 10% irregular shapes, Figure 2 below shows

electron microscopy images of our gold

nanoparticle. Note the consistency of size and shape.

Innova Biosciences’ 10nm gold nanoparticles

Leading competitor 10nm gold nanoparticles

Figure 2. TEM example pictures showing the high consistency of size and shape of Innova Biosciences’ gold nanoparticles (top)

compared to that of aggregated, irregular shaped and sized gold nanoparticles of a leading competitor (bottom).


Our 20nm colloidal gold is an aqueous suspension of spherical metallic nanoparticles manufactured to the

highest standard to ensure:



Ultra high quality




Our colloidal gold nanoparticles are suspended in citrate

buffer and they are suitable for:

Protein absorption



Size distribution and shape

Narrow size distribution and consistent shape is important and preferable for most applications.

A consistent, spherical shape means that particles are less

prone to aggregation which can be a problem associated

with gold nanoparticles. Furthermore uniform shape

ensures consistent binding of antibodies, ensuring reliable

assay results.






Figure 1. Size distribution of our 20nm gold nanoparticles



We can ensure our gold nanoparticles have less than 10% irregular shapes.

Figure 2 below shows electron microscopy images of our gold nanoparticles compared to those of a leading

competitor. Note the consistency of size and shape of our gold nanoparticles in comparison to those of the

leading competitor.



Figure 2.TEM example pictures showing the high consistency of size and shape of Innova Biosciences' gold nanoparticles (top)



Our 40nm colloidal gold is an aqueous suspension of spherical metallic

nanoparticles manufactured to the highest standard to ensure:



Ultra high quality




Our colloidal gold nanoparticles are suspended in

citrate buffer and they are suitable for:

Protein absorption




Narrow size distribution and consistent shape is

important and preferable for most applications.

A consistent, spherical shape means that particles are

less prone to aggregation which can be a problem

associated with gold nanoparticles. Furthermore

uniform shape ensures consistent binding of

antibodies, ensuring reliable assay results.

Our 40nm gold nanoparticles have a narrow size distribution (Figure 1.), with a CV (standard

deviation/diameter) of less than 10%. Each batch of 40nm colloidal gold undergoes TEM analysis to ensure

all the requirements are met.

Mean diameter SD CV%

36-44nm <4nm <10%




Figure 2 below shows electron microscopy

images of our gold nanoparticle. Note the

consistency of size and shape.

Figure 2.TEM example pictures showing the high consistency of size and shape of Innova Biosciences' gold nanoparticles


Our 80nm colloidal gold is an aqueous suspension of spherical metallic nanoparticles manufactured to the

highest standard to ensure:



Ultra high quality




Our colloidal gold nanoparticles are suspended in

citrate buffer and they are suitable for:

Protein absorption




Narrow size distribution and consistent shape is

important and preferable for most applications.

A consistent, spherical shape means that particles are

less prone to aggregation which can be a problem

associated with gold nanoparticles. Furthermore

uniform shape ensures consistent binding of

antibodies, ensuring reliable assay results.






We can ensure our gold nanoparticles have less than

10% irregular shapes, Figure 2 below shows electron

microscopy images of our gold nanoparticles

compared to those of a leading competitor. Note the

consistency of size and shape of our gold

nanoparticles in comparison to those of the leading

competitor.






Figure 2.TEM example pictures showing the high consistency of size and shape of Innova Biosciences' gold nanoparticles (top)


More technical information

Gold nanoparticles: Strange properties

and applications in the Life Sciences

Gold nanoparticles:

Optimisation of Conjugates

https://www.innovabiosciences.com/bioconjugation-and-immunoassay-webinars/gold-nanoparticles-applications.html


https://www.innovabiosciences.com/bioconjugation-and-immunoassay-webinars/gold-nanoparticles-optimization-of-conjugates.html






InnovaCoat® GOLD conjugates

Our pre-conjugated gold nanoparticles are manufactured using our InnovaCoat® GOLD nanoparticles which

have a proprietary surface coat that covalently bind the detection protein providing highly stable conjugates.

Gold nanoparticle conjugates Features and Benefits

Features Benefits

Quick and easy to use Save time, no specialist knowledge

required

Based on Innova Biosciences’ Lightning-Link® and

InnovaCoat® GOLD technologies Successful trademarks, high quality

Covalent binding between the specificity and detection

moieties Highly stable conjugates

Range of secondary reagents available Flexible

Unique conjugates available No competitors

Scalable Easy transfer from R&D to manufacturing



Available gold nanoparticle conjugates:

Colloidal Gold nanoparticles 10nm 20nm 40nm 80nm Target

Group

InnovaCoat® GOLD Streptavidin ● ● Biotin

InnovaCoat® GOLD Biotin ● Streptavidin

InnovaCoat® GOLD Goat Anti-Mouse ● ● ● ● Mouse IgG

InnovaCoat® GOLD Goat Anti-Rabbit ● ● ● ● Rabbit IgG

InnovaCoat® GOLD Protein A ● ● IgG

InnovaCoat® GOLD Protein G ● ● IgG

Want to conjugate your own antibody, protein or other biomolecule?

We also have gold nanoparticle conjugation kits allowing the easy conjugation of gold nanoparticles for use

in R&D applications and for the development and manufacture of diagnostic kits.

Please do not hesitate to contact us to find out more!

Associated documents (MSDS) MSDS on inquire


Related and other products or documents

●Associated product:

PH-BB189i AbPure™ Antibody Purification (Magnetic)

●Back to Products HighLights (overview)[] and on-line BioSciences Innovations

Information inquire

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http://www.interchim.fr/ft/B/BB189i.pdf

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Documents

Innova GOLD Nanoparticles, Conjugation kits and Conjugates › ft › B › BB100i.pdf · 2017-02-16 · Innova GOLD Nanoparticles, Conjugation kits and Conjugates Colloidal gold