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IMMUNOPHENOTYPING LEUKEMIAS AND LYMPHOMAS. 0. 256. 512. 768. 1024. 0. 1. 2. 3. 4. 10. 0. 10. 1. 10. 2. 10. 3. 10. 4. 10. 10. 10. 10. 10. 0. 256. 512. 768. 1024. SSC ->. HLADr ->. SSC ->. HLADr ->. /6/05133061. /6/05133061. /7/06064121. /7/06064121. - PowerPoint PPT Presentation
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IMMUNOPHENOTYPING LEUKEMIAS AND
LYMPHOMASRPCI
LFC
0 256 512 768 1024
/7/06064121SSC ->
0 256 512 768 1024
/6/05133061SSC ->
CD45 Gating Strategy
NORMAL BONE MARROW LEUKEMIC (TALL) BONE MARROW
10 10 10 10 100 1 2 3 4
/6/05133061HLADr ->
10 10 10 10 100 1 2 3 4
/7/06064121HLADr ->
A B C D
erythrocytes and platelets
granulocytes monocytes and B-cells
HLADr
CD
45
HLADr
CD
45
HLADr
CD
45
A
B
C
CD45 EXPRESSION BY ACUTE B-LINEAGE LEUKEMIA
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->R5
5 6
7 810 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 4
CD14
CD45 HLADr
CD14
B LINEAGE ACUTE LEUKEMIA
HLADr
CD45
HLADr
CD45
CD45
CD14
HLADr
CD14
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 810 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 4
CD19
CD5 CD10
CD19
CD10
CD5
CD10
CD5
CD5
CD19
CD10
CD19
10 1 10 2 10 3 10 4
1
10
2
10
34
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
B LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
CD34
CD5 CD19
CD34
CD19
CD5
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->R5
5 6
7 810 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 4
CD19
CD5
CD5
CD34
CD19
CD34
B LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 810 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 4
CD11c
CD20 CD22
CD11c
CD22
CD20
CD22
CD20
CD22
CD11c
CD22
CD11c
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
B LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 810 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 4
CD13
CD33 CD19
CD13
B LINEAGE ACUTE LEUKEMIA
CD19
CD33
CD19
CD33
CD33
CD13
CD19
CD13
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
1
310 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
5
7 8
CD19
CD10
CD19
CD20
CD45
CD22
HLADr
CD34
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 410 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->R5
5 6
7 8
CD14
CD45 HLADr
CD14
HLADr
CD45
HLADr
CD45
CD45
CD14
HLADr
CD14
T LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
1-Heig
ht -->
R55 6
7 810 1 10 2 10 3 10 4
FL1-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
R11 2
3 4
CD4
CD4
T LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL1 -->
10
1
10
2
10
3
10
4
R11 2
3 410 1 10 2 10 3 10 4
FL3 -->
10
1
10
2
10
3
10
4
FL
1 --
>
5
7 810 1 10 2 10 3 10 4
FL3 -->
10
1
10
2
10
3
10
4
CD8
CD3
CD3
CD4
CD8
CD4
CD3 CD8 CD8
CD3
10 1 10 2 10 3 10 4
FL1 -->
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL3 -->
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL3 -->
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 410 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 8
CD19
CD7 CD2
CD19
CD2
CD7
CD2
CD7
CD7
CD19
CD2
CD19
T LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 410 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 8
CD19
CD5 CD10
CD19
CD10
CD5
CD10
CD5
CD5
CD19
CD10
CD19
10 1 10 2 10 3 10 4
1
10
2
10
34
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
T LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 810 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
CD34
CD5 CD19
CD34
CD19
CD5
CD19
CD5
CD5
CD34
CD19
CD34
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4T LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
1-Heig
ht -->
R55 6
7 810 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
10 1 10 2 10 3 10 4
FL1-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
R11 2
3 4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
CD14
CD45 HLADr
CD14
ACUTE MYELOID LEUKEMIA
HLADr
CD45
HLADr
CD45
CD45
CD14
HLADr
CD14
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
10 1 10 2 10 3 10 4
FL1-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
R11 2
3 410 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
1-Heig
ht -->
R55 6
7 8
ACUTE MYELOID LEUKEMIA
CD33
CD11b
CD11b
CD13
CD33
CD13
CD13
CD11b CD33 CD33
CD11b
CD13
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
1-Heig
ht -->
R55 6
7 810 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
10 1 10 2 10 3 10 4
FL1-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
R11 2
3 4
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 410 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 8
ACUTE MYELOID LEUKEMIA
CD56
CD15
CD15
CD34
CD56
CD34
CD34
CD15 CD56 CD56
CD15
CD34
10 1 10 2 10 3 10 4
FL1-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
R11 2
3 410 1 10 2 10 3 10 4
FL3-Height -->10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
1-Heig
ht -->
R55 6
7 8
ACUTE MYELOID LEUKEMIA
CD2
CD7
CD7
CD13
CD2
CD13
CD13
CD7 CD2 CD2
CD7
CD13
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
1-Heig
ht -->
R55 6
7 810 1 10 2 10 3 10 4
FL3-Height -->10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
10 1 10 2 10 3 10 4
FL1-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
R11 2
3 4
ACUTE MYELOID LEUKEMIA
HLADr
CD38
CD38
CD34
HLADr
CD34
CD34
CD38 HLADr HLADr
CD38
CD34
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
8
10 1 10 2 10 3 10 4
Fluorescence One Height -->
10
1
10
2
10
3
10
4
Fluorescence Two Height -->
R1
1 2
3 410 1 10 2 10 3 10 4
Fluorescence Three Height -->
10
1
10
2
10
3
10
4
Fluorescence One Height -->
R5
5 6
7 810 1 10 2 10 3 10 4
Fluorescence One Height -->
10
1
10
2
10
3
10
4
Fluorescence Two Height -->
R1
1 2
3 4
10 1 10 2 10 3 10 4
FL1-Height -->
10
1
10
2
10
3
10
4
FL2-Height --> R1
1 2
3 410 1 10 2 10 3 10 4
FL3-Height -->
10
1
10
2
10
3
10
4
FL1-Height --> R5
5 6
7 810 1 10 2 10 3 10 4
FL1-Height -->
10
1
10
2
10
3
10
4
FL2-Height --> R1
1 2
3 4
A
D E
B
F
C
CD19
CD19
CD20
CD22
LAMBDA
LAMBDA
CD22
CD20CD5
KAPPA
KAPPA
CD5
Typical B-cell Lymphoma
10 1 10 2 10 3 10 4
Fluorescence Three Height -->
10
1
10
2
10
3
10
4
Fluorescence Two Height -->
10 1 10 2 10 3 10 4
Fluorescence One Height -->
10
1
10
2
10
3
10
4
Fluorescence Two Height -->
R1
1 2
3 410 1 10 2 10 3 10 4
Fluorescence Three Height -->
10
1
10
2
10
3
10
4
Fluorescence Two Height -->
10 1 10 2 10 3 10 4
CD19 -->
10
1
10
2
10
3
10
4
CD13 -->
10 1 10 2 10 3 10 4
FL3-Height -->
10
1
10
2
10
3
10
4
FL2-Height -->
10 1 10 2 10 3 10 4
Fluorescence One Height -->
10
1
10
2
10
3
10
4
Fluorescence Two Height -->
R1
1 2
3 410 1 10 2 10 3 10 4
FL3-Height -->
10
1
10
2
10
3
10
4
FL2-Height -->
10 1 10 2 10 3 10 4
CD19 -->
10
1
10
2
10
3
10
4
CD13 -->
A B C D
E F G H
CD19
CD19
CD19
CD13
CD19
CD13
CD19
CD19
CD2
CD2 CD7 CD10
CD7 CD10
CD19
CD19
T, CD10, and Myeloid Antigen Coexpression
in B-cell lymphoma
Lymphoma
10 1 10 2 10 3 10 4
KAPPA -->
10
1
10
2
10
3
10
4
LAMBDA -->R1
1 2
3 410 1 10 2 10 3 10 4
CD19 -->
10
1
10
2
10
3
10
4
LAMBDA -->
10 1 10 2 10 3 10 4
CD19 -->
10
1
10
2
10
3
10
4
KAPPA -->
R5
5 6
7 8
10 1 10 2 10 3 10 4
KAPPA -->
10
1
10
2
10
3
10
4
LAMBDA -->R1
1 2
3 410 1 10 2 10 3 10 4
CD19 -->
10
1
10
2
10
3
10
4
LAMBDA -->
10 1 10 2 10 3 10 4
CD19 -->
10
1
10
2
10
3
10
4
KAPPA -->
R5
5 6
7 8
CD2
CD2CD8
CD8CD3
CD3
CD7
CD7
CD4
CD4
CD4
CD4
A
FED
CB
Reactive Lymph Node
Typical T-cell Lymphoma or Reactive Lymph Node:
Problem Case
A B C
D E FKappa CD19 CD19
CD33 CD19 CD19
CD13
Kappa
Lambda
Lambda
CD33
CD13
