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REVIEW IN
RHEUMATOLOGY
Daphna Paran M.D
Dept. of Rheumatology
Tel Aviv Medical Center
Case 1
A 30 y old woman
April 2010: Nasal discharge and dry cough
1 month later:
o Painful swollen knee & ankle
o Rash
o Inflamed red eye
Laboratory tests
GLU 85
CREAT 0.9
Na 141
K 3.9
Ca 9.7
Prot 63
Alb 38
ALT 17
ALP 21
LDH 500
Hb 11.4
MCV 81
WBC 23000
neu 84%
lym 10%
PLT 265
Ferritin 89
ESR 56
CRP 11.7
Additional workup
• Skin biopsy - Leukocytoclastic vasculitis
• Ophthlamologic examination- Episcleritis
• Working diagnosis:
• Denies: oral ulcers, ear inflammation, sinusitis,
chest pain, dyspnea, GI symptoms, paresthesias
Susp Systemic Vasculitis
Management: Prednisone 1mg/kg
Additional Laboratory tests
Urine Prot 717mg/d
Urine sed RBC
Hepatitis B neg
Hepatitis C neg
RF 33
ANA neg
C3 159
C4 46.8
C- ANCA 1:160
Anti-PR3 157
P-ANCA neg
Anti-MPO neg
Imaging studies
CT of sinuses: mild opacification in maxillary sinus
Chest CT: single lesion with irregular borders in RLL
m/p pulmonary nodule
• Kidney biopsy- crescentic glomerulonephritis
1.5 month later
Hb 11.6
Creat 0.7
ESR 6
CRP <0.5
Urine Protein 1300 mg/24h
Summary
• Nasal discharge
• Polyarthritis
• Lung nodule
• Skin - vasculitis
• Eyes - episcleritis
• Renal involvement - Proteinuria , Hematuria
• Positive Serology- C-ANCA+ anti-PR3 +
• Elevated ESR and CRP, leukocytosis
AAV= ANCA associated Systemic Vasculitis
ANCA
Antineutrophil cytoplasmic antibodies
• Combined testing is recommended :
- indirect immunofluorescence (IIF) assays
- enzyme immunoassays (EIAs) for:
anti-proteinase 3 (anti-PR3); anti-myeloperoxidase (anti-MPO)
• Most reliable results : positivity by both IF and EIA
• Positive serologies alone are not diagnostic of vasculitis
• Negative ANCA does not preclude diagnosis of vasculitis (10%)
ANCA detection by Immunofluorescence
Cytoplasmic staining = C-ANCA Perinuclear staining = P-ANCA
Atypical ANCA : may be seen in: CTDs, IBD, autoimmune hepatitis
may be confused with P-ANCA patterns
ANCA- C, P
C - ANCA
Proteinase 3
P- ANCA
Myeloperoxidase
Elastase
Cathepsin 3
Lactoferrin
BPI
c ANCA p ANCA
• GPA )Wegener( 90%
• Infectious disorders
• Neoplasms
• MPA )Microscopic polyangiitis( 70%
• Rapidly progressive GN
• Churg Strauss syndrome -50%
• Rheumatoid arthritis
• SLE
• JRA
• IBD
• Drug induced lupus
ANCA-Associated Vasculitis
• Granulomatosis with polyangiitis = GPA
(Wegener’s granulomatosis)
• Microscopic polyangiitis = MPA
• Eosinophilic Granulomatosis with polyangiitis = EGPA
(Churg-Strauss syndrome)
• Renal-limited vasculitis:
pauci-immune necrotizing GN with little or no deposition of Ig and
complement
75 to 80 % have MPO-ANCA
Granulomatosis with polyangiitis
Chapell Hill Classification
• Granulomatous inflammation of respiratory tract and necrotizing
vasculitis of small to medium-sized vessels
capillaries, venules, arterioles, arteries
• Necrotizing glomerulonephritis is common
• GPA may affect virtually any organ system
predilection for the upper respiratory tract, lungs, kidneys
ACR 1990 classification criteria
• Nasal or oral inflammation
• Abnormal chest radiograph
• Urinary sediment
• Granulomatous inflammation on biopsy
The cornerstone of diagnosis in GPA:
A combination of typical clinical features
and histopathology
Back to Case
Management dilemmas
• Kidney biopsy?
• Treatment?
Kidney biopsy
Urine Protein- 1300 mg/24h
Active urine sediment
Kidney Biopsy: Extracapillary hypercellularity,
fibrocellular crescents and focal sclerosis
Pauci-immune crescentic glomerulonephritis
Treatment dictated by severity
Limited disease
• conductive hearing loss
• sinus disease
• cutaneous lesions
• arthritis/arthralgia
• pulmonary nodules or
infiltrates that do not
compromise lung function
significantly
Severe disease
• rapidly progressive GN
• alveolar hemorrhage
• intestinal ischemia
• necrotizing scleritis
• retro-orbital pseudotumor
• vasculitic neuropathy
Disease assessment
• Birmingham Vasculitis Activity Score for WG (BVAS/WG)
• The Vasculitis Damage Index
Milestones in the treatment of GPA
• 1950: GPA is a fatal disease - 5 month survival
• 1960: GC increased survival to 12.5 months
• 1970: Break through - NIH protocol with oral CYC
• 1997-98 : IV CYC
• 1999 : Induction with CYC, maintenance MTX
• 2003 : Induction with CYC, maintenance AZA
• 2005: MTX up to 25 mg/wk as good as oral CYC
in early AAV without critical organ disease
• 2009: CYCLOPS study:
pulse CYC 15 mg/kg q2 -3 weeks = oral CYC 2mg/kg/d plus steroids
• 2010: Rituximab
Rituximab (MabThera): CD20 Antigen as a Target for Immunotherapy
ANTIGEN INDEPENDENT ANTIGEN DEPENDENT
CD20
expression
Neoplasias: Precursor B-cell leukemias B-Cell lymphomas/CLL WM/
Myeloma
Adapted from Longo. Lymphocytic Lymphomas. In: Cancer: Principles and Practice of Oncology. 1993.
Stem
Cell
Pre-Pre-
B Cell Pre-B Cell
Immature
B Cell
Mature
B Cell
Activated
B Cell
Plasma
Cell
IgM
IgG
IgA
sIgM
sIgG
sIgA
sIgM
+ D sIgM
HCR
R/D
HCR
R/D µ HCR
HCR
Rituximab Depletion of B cells
n=166
McLaughlin et al. J Clin Oncol. 1998;16:2825.
Indications in Rheumatology
FDA approved
• Rheumatoid Arthritis
• Granulomatosis with polyangiitis
• Microscopic polyangiitis
Off label
• SLE
• Primary Sjogren syndrome
FDA News Release: April 19, 2011
• The FDA approved Rituximab with glucocorticoids
as front-line therapy for adult patients with GPA or MPA
• The first FDA-approved therapy for these diseases and
the first alternative to CYC for the treatment of severe
disease in almost 40 years!
• Rituximab -approved in Israel for AAV in :
- patients who failed cyclophosphamide treatment
- young women of childbearing age
Rationale for targeting B cells in AAV
In AAV:
• B cells have important role in pathogenesis
• B-cell activation correlates with disease activity
BAFF levels (B-cell activating factor) correlate with activity
• Effects of CYC on B lymphocytes are associated with efficacy
RAVE study
• Multicenter, randomized, double-blind non-inferiority trial
for induction of remission in severe AAV
Rituximab vs. Cyclophosphamide
• Inclusion criteria:
- GPA or MPA
- Severe disease: BVAS/WG ≥ 3
End Points
Primary
• BVAS/WG of 0 and
successful prednisone taper at 6 months
Secondary
• Rates of disease flares
• BVAS/WG of 0 with prednisone at <10 mg/day
• Cumulative glucocorticoid doses
• Rates of adverse events
• SF-36 scores
Conclusions
• Treatment with Rituximab and glucocorticoids :
Not inferior to the standard regimen in severe
AAV of recent onset
• Rituximab and glucocorticoids:
May be superior to the standard regimen for
remission induction in severe relapsing AAV
• No difference in adverse events
Standard of Care
Limited disease
• MTX, Azathioprine
• Glucocorticoids
Severe disease
• Induction : High dose CS +CYC or Rituximab
• Maintenance with MTX or azathioprine or Rituximab
Septrin
• Controversial effect in GPA
• May have a role in limited GPA in addition to other treatment
• Indicated for prevention of PCP
Back to case - Management
• T. Prednisone 60 mg/d
• IV Cyclophosphamide (CYCLOPS protocol)
• T. Septrim forte 1x3/wk
• Vaccination - Influenza, Strep Pneumonia
Initial response
Follow-up (6 mo)
• CYC (x7) + Prednisone tapering to 30 mg/d :
• Recurrence of episcleritis
• Skin rash
• Myalgia
• Proteinuria
• Increased PR3 (200)
• Consequent dose increase Prednisone 70 mg/d
Management
• Rituximab 1000 mg x2
Prompt improvement:
• normal urinalysis
• resolution of pulmonary nodule, sinusitis, rash, episcleritis
• decreased PR3
• Maintenance treatment - Rituximab
• Current treatment - Prednisone 5 mg/d
- Maintenance with methotrexate
• Single course of rituximab vs. CYC followed by AZA (n=197)
(long-term results of RAVE trial)
• Results:
similar rate of complete remission by 6 months,
maintained through 18 months.
• Conclusion:
In severe AAV- single course of rituximab as effective
as continuous immunosuppression for induction and
maintenance of remission over course of 18 months
Efficacy of Remission-Induction Regimens for
ANCA-Associated Vasculitis NEJM August 2013
MAINRITSAN
Protocol:
• Induction: CYC + GCs (n=115)
• Maintenance: AZA (n=58)
RTX (n=57)
AZA: 2mg/kg/d- 12 mo., 1.5mg/kg/d- 6 mo., 1m/kg/d- 4 mo.
RTX: 500mg at day 0,14, then every 6 mo. upto 18 mo.
Relapse rate: RTX- 5% vs AZA-29%
EGPA - Eosinophilic Granulomatosis with PolyAngiitis
( Churg- Strauss syndrome)
EGPA: evolutions in classification, etiopathogenesis, assessment and management.
Curr Opin Rheumatol 2014
ANCA associated vasculitis- ANCA positive in 30-70% of cases
Hypereosinophilic condition - frequent lung involvement (90%)
Eosinophilic vasculitis
Damage due to vasculitis and proliferation of eosinophils
Mepolizumab (Nucala) = anti- IL-5
IL-5 - has a central role in eosinophil recruitment
Mepolizumab - a humanized monoclonal antibody against IL-5
Mepolizumab has been approved by the FDA and NICE for:
add-on, maintenance treatment of severe asthma in patients
age 12 or older who have an eosinophilic phenotype
Inclusion criteria-history of relapse or refractory
disease
History of RELAPSE or REFRACTORY DISEASE
RELAPSING DISEASE
Past history of at least one confirmed
EGPA relapse
• requiring increase in CS dose;
initiation/increased dose of
immunosuppressive therapy or
hospitalisation
• occurred within the past 2 years, but at
least 12 weeks prior to the screening
visit, while receiving a dose of
prednisolone of ≥7.5 mg/day
REFRACTORY
DISEASE
Failure to attain remission
(BVAS=0 and CS dose ≤7.5 mg/day) following induction
treatment with a standard regimen, for at least 3 months
• recurrence whilst tapering CS
• occurring at any prednisone dose ≥7.5 mg/day
OR
40
Wechsler ME et al. NEJM 2017 Supplementary Appendix
Remission: BVAS=0 and OCS dose ≤4 mg/day
Results for the ITT population
Proportion of Subjects Who
Achieved Remission at Each Visit
Last dose of study treatment
Wechsler ME et al. NEJM 2017
SC Mepolizumab 300mg
q4wks + standard care
vs
Placebo + standard care
The time to first relapse was significantly
longer for patients treated with mepolizumab
vs placebo
Percentage of patients with confirmed EGPA relapse
100
90
80
70
60
50
40
30
20
10
0
0 4 8 12 16 20 24 28 32 36 40 44 48 52
Pa
tie
nts
wit
h r
ela
ps
e (
%)
Weeks to event
Hazard ratio: 0.32
95% CI: 0.21–0.50
P<0.001
Placebo
Mepolizuma
b
82% of placebo patients experienced a relapse
56% of mepolizumab patients experienced a relapse
68% RRR* in relapse
26% ARR† in relapse
*RRR = Relative risk reduction;
†ARR = Absolute risk reduction
68 33 16 9
68 55 43 25
Placebo
Mepolizumab
No. at risk
Wechsler ME et al. NEJM 2017
Average prednisone dose
18
26
15
41
3 4
26
66
0
10
20
30
40
50
60
70
0 >0 to 4.0 >4.0 to 7.5 >7.5
% o
f su
bje
cts
Average CS dose (mg/day)
Average Prednisone Dose mg/day Weeks 48-52
Nucala 300 mg Placebo
Nucala vs placebo:
OR, 0.20 (95% CI: 0.09, 0.41)
P < 0.001
Mepolizumab:
• allowed reduction in prednisone dose
• reduced relapse
• increased chance of remission at 36 ,48 wks
Case 2
A 72 year old lady with:
• History of DM and hypertension
• Severe occipital and temporal/frontal pain for more
than 6 months, weight loss
• Treated in pain clinic because of suspected cervical
radiculopathy - no improvement
• Sub-febrile fever, up to 38 degrees for the past 2 months
16% of FUO cases at age > 65 years are due to GCA
Giant Cell Arteritis
• GCA - the most common form of systemic vasculitis in adults
• Age > 50 years, mean age- 72
• Females/ Males - 3 : 1
• Northern European ancestry (Caucasians)
• Incidence : 240/1 million
Systemic vasculitis
• Fever (42%)
16% of FUO cases at age > 65 years are due to GCA
• Anorexia and weight loss (43%)
• Fatigue (42%)
• Arthralgias/arthritis
• Polymyalgia Rheumatica - PMR (35%)
Clinical manifestations of GCA
Frequency (%) Symptoms
76 Headache
37 Any visual symptom
34 Jaw claudication
24 Unilateral visual loss
15 Bilateral visual loss
11 Vertigo
9 Diplopia
Unexplained fever, anemia, or other constitutional symptoms and signs
Assessment
• Clinical symptoms
• Laboratory tests:
• ESR ,CRP, CBC
• Imaging:
• US of temporal arteries
• CT angiography
• PET-CT
US in diagnosis of temporal arteritis (GCA)
CTA in diagnosis of GCA
PET-CT in diagnosis of GCA
Pathology
Pan-arteritis, most pronounced in media, fragmented internal elastic lamina
Inflammatory infiltrate of CD4+ lymphocytes and macrophages
Giant cells are common but
not required for diagnosis
Fibrinoid necrosis suggests
systemic necrotizing vasculitis
1990 criteria for classification of GCA
(at least 3 of 5)
Criterion Definition__________________
1. Age at onset>50years symptoms beginning at >50 yrs
2. New headache new onset or new type of
localized pain in head
3. Temporal artery abnormality tenderness or decreased pulsation
4. Elevated ESR ESR >50mm/h
5. Abnormal artery biopsy vasculitis with predominant
mononuclear infiltrate or
granulomatous inflammation,
usually with giant cells
New classification criteria for GCA
Clinical features: Imaging findings:
Morning stiffness shoulder/neck +2 Halo sign +5
Sudden visual loss +2 Bilateral axillary findings +3
Jaw claudication +2 PET activity in aorta +3
New temporal headache +2
Scalp tenderness +2
Laboratory:
ESR >50 or CRP>10mg/l +3
Temporal artery biopsy:
Definite vasculitis +5
Possible vasculitis +2
Classified as GCA:
Score>6
Treatment
• Glucocorticoids
• 40 -60 mg/d
• solumedrol pulses
• solumedrol pulses and lower doses of oral GCs
• Measures to prevent osteoporosis and adverse effects of GCs
• Aspirin
• Retrospective studies
• Decreases vascular events
Glucocorticoid protocol
• Prednisone: 40-60 mg/d until resolution of symptoms and APR
• Reduction by 10 mg at 2-week intervals until 20 mg
• Reduction by 2.5 mg at 2-week intervals until 10 mg
• Reduction by 1 mg per month every 4 - 8 weeks
• Most patients stop treatment by 2 years
Back to our patient
• Treated with prednisone 1 mg/kg
• Dramatic improvement in her symptoms
• However …
• Reduction of ESR and CRP but no normalization after 4 weeks of
therapy
• Still has headache
• Uncontrolled DM and hypertension
Glucocorticoid sparing agents
• MTX
• Leflunomide (Arava)
• Azathiopine (Imuran)
• Cyclophophamide
• Biologics
Study design
Primary endpoint: sustained prednisone free remission at 52wks in each TCZ group vs. PLB + 26-week prednisone
Secondary endpoint: 1. sustained prednisone free remission at 52wks in each TCZ group vs. PLB + 52-week prednisone
2. incidence of first flare after remission
3. cumulative prednisone dose
Back to our patient
• Started tocilizumab
• Complete remission , without CS
• N=49 received prednisone + abatacept
• At 12 weeks 2 arms: continued abatacept + prednisone vs. prednisone
• Both arms tapered prednisone and stopped at 28 weeks
• Relapse free survival at 12 months 48% vs. 31% (p=0.049)
Case 3
Young single woman
Presents at age 16 with:
• Arthritis
• Photosensitive rash
Lab tests:
• CBC, liver and kidney function tests, albumin- normal
• Urinalysis-normal
• ESR 42/, CRP- 0.6
• ANA+, anti-dsDNA-negative, ENAs-negative
• decreased C3
SLE ?
SLE: 1982 classification criteria - requires 4 of 11
1. Malar rash
2. Discoid rash
3. Photosensitivity
4. Oral or nasopharyngeal ulcers
5. Arthritis
6. Serositis
7. Renal disorder - proteinuria>0.5g/d or cellular casts
8. Neurologic disorder - seizures or psychosis
9. Hematologic - hemolytic anemia/ leukopenia/ lymphopenia/
thrombocytopenia
10. Immunologic disorder- anti-dsDNA/anti-Sm /APLA
11. Antinuclear antibody positive
New SLE preliminary criteria
1. Acute cutaneous lupus
2. Chronic cutaneous lupus
3. Oral or nasal ulcers
4. Non scarring alopecia
5. Synovitis
6. Serositis
7. Renal
8. Neurologic
9. Leukopenia
10. Hemolytic anemia
11. Thrombocytopenia
12. Immunological:
- ANA
- Anti ds DNA
- Anti Sm
- APLA
- Low Complement
- Coombs +
New SLE preliminary criteria
• At least 4 criteria:
at least 1 clinical and 1 immunologic
OR
• Biopsy-proven lupus nephritis in the presence of
ANA or anti-dsDNA antibodies
ACR/EULAR 2018
classification criteria
- Immunofluoresence assay (IFA)
- ELISA
- Multiplex bead assays
Antinuclear antibody
Anti-dsDNA
• Radioimmunoassay (the Farr assay)
• Crithidia luciliae kinetoplast staining assay
• ELISA
• indirect IFA on the flagellate Crithidia luciliae
• specific method to detect anti-dsDNA
• positive reaction = fluorescent kinetoplast
• Kinetoplast= network of tightly packed dsDNA
within the mitochondrion, free of nuclear proteins
Case 3- course
At age 16 :
• Arthritis, Photosensitive rash
Treatment: Hydroxychloroquine
At age 20 :
• Hemolytic anemia
• Thrombocytopenia
• Vasculitic rash
Treatment:
• Hydroxychloroquine
• Corticosteroids
• Azathioprine
• Avascular Necrosis of hip THR
Good response
Treatment of Non-renal lupus
Anti- Malarials: Hydroxychloroquine = Plaquenil
Effective for:
- Constitutional symptoms, fever
- Rash
- Arthritis
Steroid sparing
Prevents flares
Increases survival
anti-aggregant effect
Treatment of Non-renal lupus
• NSAIDs
• Low-dose Corticosteroids
• Immunosuppressive agents
MTX, Leflunomide- for arthritis, serositis
Azathioprine, Mycophenolate mofetil
• Biologics :
Belimumab
1992:
• Young 19 year old woman
• polyarthritis, pleuritis, pericarditis, fever
• Laboratory tests:
- Leukopenia- 3000
- Lymphopenia- 900
- ANA +, anti dsDNA+, low C3
Case 4
1992-1997:
- Plaquenil- partial improvement
- Recurrent episodes of pleuritis/pericarditis
- NSAIDs - No response
- Prednisone 20-30mg/d - steroid dependent
- IM Methotrexate 15-20mg/wk - partial response
Case continued
1997
Multiple Splenic infarcts
anticardiolipin IgG, lupus anticoagulant- positive
Warfarin treatment , no additional thrombotic episodes
2000
Planned pregnancy
Methotrexate switched to Imuran
2000-2008-
Four normal pregnancies with full term healthy babies
Rx during pregnancies:
T Plaquenil
T Imuran100mg/d
T Prednisone 10mg/d
SC Clexane 60mg x2/d
No flares during pregnancy
2008-
Postpartum flare with severe polyarthritis
Requiring prednisone 15-30mg/d
Switched back to Methotrexate with no improvement
Case report continued
2008-2012:
Unable to achieve remission -
polyarthritis with 6-8 swollen and tender joints despite:
Plaquenil + Methotrexate 15mg/wk + Prednisone 15mg/d
5/2012:
Severe pleuritic chest pain-
requires prednisone increase -35mg/d
Case continued
In Summary:
- Active SLE with polyarthritis, pleuritis and pericarditis
- Poor response to Plaquenil with Imuran or Methotrexate
- Steroid dependent for many years
- Unable to reduce prednisone below 12.5mg/d
- Developed significant osteoporosis
Started treatment with Benlysta (anti BLyS = Belimumab)
After 4 months able to reduce prednisone to 7.5 mg/d
Ag
TCR MHC
class II
.
Cytokines Cytokines
B cell T cell
BLyS
Belimumab
Belimumab-blocks BLyS -
a B cell survival factor
B Lymphocyte Stimulating Protein
• BLyS is over expressed in SLE
• BLyS levels correlate with:
• changes in disease activity
• anti-ds DNA antibody titers
BLISS Study Design multicentre randomized double-blind placebo-controlled phase 3 trials
Dosing: d 0, 14, 28, then every 28 d through wk 48/72, final evaluation at wk 52/76
Stratified by:
- screening SELENA-SLEDAI: 6–9 vs ≥10
- screening proteinuria: <2 vs ≥2 g/24 h
- race: African descent or indigenous American vs other
Primary endpoint: SLE Responder Index (SRI) at wk 52
aNavarra SV et al. Lancet. 2011;377;721-31; bFurie RA et al. Arthritis Rheum. 2010;62(suppl):S606, Abstract 1454; cFurie RA et al. Arthritis Care
Res. 2009;61:1143-51.
Placebo
+ routine therapy
Belimumab 1 mg/kg + routine therapy
Belimumab 10 mg/kg + routine therapy
• SELENA-SLEDAI (SS) ≥6
• Seropositive:
ANA ≥1:80 and/or anti-dsDNA ≥30 IU/mL
• No active severe lupus nephritis or
severe CNS lupus
• Stable routine SLE therapy ≥30 d
• Progressive restrictions on concurrent
medications
Treated:
BLISS-
52
n= 865
BLISS-
76
n = 819
Primary Efficacy Endpoint SLE Responder Index (SRI)
Response defined as:
> 4 point reduction from baseline in SELENA SLEDAI AND
No worsening in Physician’s global assessment (PGA)
No new BILAG A or 2 BILAG B organ domains
Primary Efficacy Results: SRI at Week 52
BLISS-52 and BLISS-76
BILAG, British Isles Lupus Assessment Group; PGA, Physician’s Global Assessment.
Petri M et al. Arthritis Rheum. 2010;62(suppl):S190, Abstract 452.
Re
sp
on
de
rs (
%)
Benlysta (Belimumab)
In SLE patients with:
- more pronounced serologic activity: low C3 C4, anti-dsDNA +
- corticosteroid use
Belimumab + routine SLE therapy significantly reduced:
- disease activity
- severe flares
- fatigue
- corticosteroid use
Back to Case 3
Age 20:
Arthritis, Malar Rash, vasculitic rash
Hemolytic anemia, thrombocytopenia Treatment: Hydroxychloroquine, low dose prednisone, AZA
Age 32:
• Fatigue, myalgia
• Leg edema
• Urinalysis: proteinuria - 3 gr/d, hematuria, RBC casts
Management of Lupus Nephritis
Joint EULAR and European Renal Association- European
Dialysis and Transplant Association
Recommendations for the management of Lupus Nephritis
Annals Rheum Dis 2012
American College of Rheumatology Guidelines for the
screening and Management of Lupus Nephritis
Arthritis Care Res 2012
• Proteinuria > 0.5 gr/d
• Especially with
glomerular hematuria and/or cellular casts
Indication for Renal Biopsy
WHO Classification of Lupus Nephritis J Am Soc Nephrol 15: 241-250, 2004
Class I - Minimal mesangial LN
Class II - Mesangial proliferative LN
Class III- Focal proliferative LN (<50% of glomeruli with focal subendothelial immune deposits)
Class IV - Diffuse proliferative segmental LN (IV-S)
- Diffuse proliferative global LN (IV-G) (> 50% of glomeruli with subendothelial immune deposits)
Class V - Membranous LN
(global or segmental subepithelial deposits)
Class VI - Advanced sclerosing LN (> 90% of glomeruli globally sclerosed)
• Activity index
• Chronicity Index
Renal disease in SLE
• Mild disease - Class II
• Serious disease - Class III, IV, V
Clinical course:
- Class II:
hematuria, sub-nephrotic proteinuria, preserved GFR
- Class III and IV:
edema, HTN
nephritic sediment, mild-mod proteinuria, acute in GFR
- Class V:
features of nephrotic syndrome, preserved/ gradual in GFR
Renal Biopsy
• Renal biopsy is essential:
- Glomerulonephritis classification ( Class I-VI)
- Activity vs. chronicity
• Other pathologies :
• Tubulo-interstitial nephritis
• Thrombotic microangiopathy
• Vasculitis
Case 3 Back to patient:
Renal Biopsy: Type IV Glomerulonephritis
Lupus Nephritis
• Affects 25% to 60% of adults with SLE
• Relapse >50%
• 10% to 30% progress to ESRD within 15 years
• X2 mortality compared to SLE without LN
• Significant morbidity due to:
• active disease
• sequelae of prolonged use of CS and IS:
• accelerated atherosclerosis
• osteoporotic fractures, DM, HTN, cataract, etc.
• infections
Treatment of Renal lupus- Induction
• Cyclophospamide :
- NIH protocol (high dose): IV 500-1000mg/m2 q month (X6)
OR
- Eurolupus (low dose): IV 500mg q 2weeks (X6)
OR
• Mycophenolate Mofetil -2-3 gr/d
• at least equivalent to CYC as induction therapy
• more favorable toxicity profile
Treatment of Renal lupus- Maintenance
AZA or MMF better maintenance therapy than CYC Contreras G et al. NEJM 2004
Treatment of Renal lupus- Maintenance
• Maintenance with either MMF or AZA well tolerated
- MMF: possibly more efficacious, especially in minorities
- AZA: drug of choice when planning pregnancy
• Extended therapy of 3-4 years protects from relapse
MAINTAIN nephritis trial ARD 2010
Maintenance phase of ALMS trial - NEJM 2011
Case 3 - Back to patient
Treatment and course:
• High dose corticosteroids
• MMF
• Resolution of proteinuria Remission
• Age 34 -switched MMF to AZA due to planned pregnancy
• Successful pregnancy
Case 5
20 year old girl with SLE since age 11
- polyarthritis, malar rash, high titer anti-dsDNA
- diffuse proliferative lupus nephritis (class IV)
Previously treated and failed to respond to:
- Plaquenil
- Prednisone 1mg/kg
- IV pulse methylprednisolone
- IV Cyclophosphamide q month
- Azathioprine
- Cellcept (MMF)
Disease course
Poor response to multiple treatments
Developed renal failure
Had persistent proteinuria: 7 grams/24 hours
Suffered severe side effects from treatment:
- marked weight gain
- cushingoid habitus
- hypertension
- psychosis
0
1
2
3
4
5
6
7
82.0
4
4 6 10
11
1.0
5
2 3 4 7 8 9 12
1.0
6
3 4
5.0
6 10.0
9
gra
ms
date
proteinuria
Stop MMF MMF 1g
fever
MMF 3g
Pred 60mg Rituximab
Pred 15mg
Pred 10mg
Stop MMF
Achieved remission!
The LUNAR trial
• phase III RCT - 144 patients
• MMF + Prednisone vs MMF +Prednisone + Rituximab in
lupus proliferative nephritis (types III/IV)
• At 52 weeks: Rituximab + MMF was not superior to MMF
BUT:
Responders RTX vs PLB 56.9% vs 45.8%
Improvement in proteinuria: RTX vs PLB: 32% vs 9%
CYC rescue at wk 52: RTX vs PLB: 0% vs 8%
Open study
LN- Class III, IV, V n=50
Rituximab 1000mg X2 +
IV Methylprednisolone 500mg
AND
MMF up to 1.5 g x2/d
At 52 weeks: CR-52% PR-34%
on days 1 and 15
Annals Rheum Dis 2013
ACR and EULAR/ ERA-EDTA guidelines for management of Class III and IV LN - 2012
Induction
IV MP 500-750mg/d for 3 days
then T Prednisone 0.5-1mg/kg
MMF 2-3gr/d OR IV CYC (high/low dose)
Maintenance
MMF 1-2gr/d OR AZA 2mg/kg/d
+ low dose pred + low dose pred
If no response
Rituximab
If no response
Case 6
• A 72 year old woman
• Joint Pain for 5 months - shoulders, wrists, MCPs
• Synovitis of 1 wrist, 2 MCPs
• Morning stiffness >1 hour
• ESR: 72, elevated CRP
• RF negative
• Anti-CCP positive (>300)
ACR criteria of RA - 1987
• Morning stiffness lasting at least 1 hour
• Soft tissue swelling of three or more joints
• Swelling of hand joints
• Symmetric swelling
• Rheumatoid nodules
• Presence of Rheumatoid Factor
• Radiographic erosions or periarticular osteopenia in hands and /or wrist joints
Definite RA 4/7 criteria
Criteria 1-4 present > 6 weeks
Copyright © 1972-2004 American College of Rheumatology Slide Collection. All rights reserved.
Rheumatoid factor in rheumatic disease
• SLE
• Systemic sclerosis
• Dermato/polymyositis
• Vasculitis
• Juvenile RA
• Rheumatoid Arthritis
• Sjogren’s syndrome
• Cryoglobulinemia
RF= autoab against Fc portion of IgG
Copyright © 1972-2004 American College of Rheumatology Slide Collection. All rights reserved.
Rheumatoid factor in non-rheumatic diseases
Normal individuals (< 5%)
Elderly
Bacterial infections
Endocarditis
Leprosy
Syphilis
Lyme disease
Viral infections
Hepatitis C (also A & B)
Parvovirus
Rubella
CMV
EBV
HIV
Parasitic diseases
Periodontal disease
Copyright © 1972-2004 American College of Rheumatology Slide Collection. All rights reserved.
Rheumatoid factor in non rheumatic diseases
• Lymphoproliferative disease
• Interstitial lung disease
• Chronic liver disease
• Sarcoidosis
• Post-vaccination
• Malignancies
Citrullination of peptides
Anti-CCP (=ACPA)
• Sensitivity : 68-89 %
• Specificity: 96-100%
• Positive in 35% of RF negative sera
Pre-clinical RA
Genetic and environmental factors
The 2010 ACR/EULAR
classification criteria for RA
A. Distribution of Joint involvement:
1 large joint- 0 2-10 large joints-1 1-3 small joints- 2
4-10 small joints-3 >10 joints (at least 1 small joint) - 5
B. Serology:
Negative RF and negative ACPA -0
Low-positive RF or low-positive ACPA- 2
High-positive RF or high-positive ACPA- 3
C. Acute-phase reactants:
Normal CRP and normal ESR- 0
Abnormal CRP or abnormal ESR- 1
D. Duration of symptoms: <6 weeks- 0 >6 weeks- 1
Definite RA
Score > 6
Treat to Target
• The primary target for treatment of RA:
clinical remission or low disease activity
• Validated composite measures
• Treatment adjustment every 3 months
TNF inhibitors- Monoclonal Abs vs. Etanercept (Infliximab, Adalimumab, Golimumab Certulizumab, vs. Etanercept )
• Efficacy in IBD and extra-articular manifestations
(uveitis, psoriasis)
• Reactivation of TB
• Immunogenicity
Non TNF inhibitor biologics
Abatacept ( Orencia) - CTLA4 Ig- Costimulation blockade
Rituximab (MabThera) - anti CD20
Tocilizumab (Actemra) - anti IL6 receptor
Small molecules
JAK Inhibitors
Tofacitinib (Xeljanz)
Baracitinib (Olumiant)
Binding of cytokines to cytokine receptors
activates JAK pathway signaling
JAK=Janus kinase; P=phosphate; STAT=signal transducer and activator of transcription. Shuai K, Liu B. Nat Rev Immunol. 2003;3(11):900-911.
Cytokine binding to its cell surface
receptor leads to receptor polymerization and
activation of associated JAKs
1
Activated JAKs phosphorylate the receptors that dock STATs
2
Activated JAKs phosphorylate STATs,
which dimerize and move to the nucleus to
activate new gene transcription
3
117
Gene transcription
JAK JAK P P
P P
STA
T
STA
T S
TA
T
STA
T
P
P
Tofacitinib is a JAK Inhibitor
119
119
Gene transcription
JAK JAK
STA
T
STA
T
JAK=Janus kinase; STAT=signal transducer and activator of transcription. 1. Shuai K, Liu B. Nat Rev Immunol. 2003;3(11):900-911; 2. Jiang JK, et al. J Med Chem. 2008;51(24):8012-8018.
Cytokine binding to its cell surface receptor
leads to receptor polymerization1
1
JAKs cannot phosphorylate the receptors that therefore cannot dock STATs
JAKs cannot phosphorylate STATs,
which cannot dimerize and move to the
nucleus to activate new gene transcription
3
Tofacitinib inhibits the autophosphorylation and
activation of JAK2
2
Targeted therapy in Rheumatology
What’s new in SpA?
Case 7
• A 25 year old man
• Insidious onset low back pain
• Night pain
• Morning Stiffness
• Improves with exercise
New set of criteria for Inflammatory Back pain
• Age at onset <40 years
• Insidious onset
• Improvement with exercise
• No improvement with rest
• Pain at night
• If 4 out of 5 parameters are fulfilled criteria have:
- sensitivity of 77.0%
- specificity of 91.7%
Rudwaleit M, et al. Arthritis Rheum. 2005;52:1000-1008.
Time (years)
Back pain Back pain
Radiographic
sacroiliitis
Back pain
Syndesmophytes
Pre-Radiographic Stage
(Undifferentiated Axial SpA)
Radiographic Stage
(AS)
Modified New York Criteria 1984
(MRI: active sacroiliitis)
AS Changes Over Time
AS Classification:
Modified New York Criteria
Clinical criteria
Requires fulfillment of 1 the
following:
• Low back pain >3 months,
improved by exercise, not
relieved by rest
• Limitation of lumbar spine
motion, sagittal and frontal
planes
• Limitation of chest expansion
relative to normal values for
age and sex
Radiographic criteria
Requires fulfillment of 1 the following:
• Sacroiliitis grade 2 bilaterally
• Sacroiliitis grade 3–4 unilaterally
van der Linden S, et al. Arthritis Rheum. 1984;27:361-368.
Definite AS
Any of the radiographic Criteria
and any clinical criterion (1-3)
Probable AS
3 clinical criteria or
1 radiological criteria
Copyright ©2009 BMJ Publishing Group Ltd.
Classification criteria for axial spondyloarthritis
education
exercise
physical therapy
rehabilitation
patient
associations
self-help groups
Nonsteroidal Anti-inflammatory Drugs (NSAIDs)
Peripheral
disease
Axial
disease
Sulfasalazine
TNF blockers
A
na
l
ge
s
i
c
s Local corticosteroids
S
u
r
g
e
r
y
Zochling J, et al. Ann Rheum Dis. 2006;65:442-452.
ASAS/EULAR Recommendations
for the Management of AS
ASA Assessment in AS; EULAR, European League Against Rheumatism
What’s new in Psoriatic
Arthritis?
Case 8
• A 25 year old man
• Pain and swelling of the 2nd digit
• Inflammatory back pain
• History of psoriasis
• Psoriatic arthritis
Moll and Wright clinical classification
• Symmetric polyarthritis: 30-60%
• Monoarticular, asymmetric oligoarticular : 20-40%
• 1 large joint
• 1 or 2 IP joints
• Dactylitis
• DIPs 5-20%
• Axial: 3-5%
• Arthritis mutilans : 5%
Treatment of PsA
Synthetic DMARDs
MTX
Leflunomide
Salazopyrine
Apremilast (Otezla)
Tofacitinib (Xeljanz)
Biologic DMARDs
TNFα blockers
IL12/23 inhibitor
Ustekinumab (Stelara)
IL-17 inhibitors:
Secukinumab (Cosentyx)
Ixekizumab (Taltz)
Brodalumab (Siliq)
Apremilast = PDE4 Inhibitor
Oral Small Molecule - works intracellularly
Modulates Pro- and Anti-inflammatory Mediators1a
cAMP
cAMP cAMP
AMP
PDE4 Apremilast
Anti-Inflammatory
Mediators
(i.e. IL-10)
Pro-Inflammatory
Mediators
(i.e. TNF-α, IL-23,
IFN-)
Immune Cell
1. Schafer P. Biochem Pharmacol. 2012;83:1583–1590.
AMP AMP
aVisual representation based on
preclinical evidence.
Apremilast is an investigational drug and is not approved for use in Europe
OTEZLA
Modified ACR 20 response over 104 weeks
141
ACR20=American College of Rheumatology 20; n/m=number of responders/number of patients with sufficient data for evaluation.
Kavanaugh A, et al. ACR 2014 [poster 1590].
PALACE 1
Patients achieving PASI-50 over 104 Weeks
142
PALACE 1
PASI=Psoriasis Area and Severity Index; BSA=body surface area; n/m=number of responders/number of
patients with sufficient data for evaluation.
Kavanaugh A, et al. ACR 2014 [poster 1590].
↑Cytokines including
IFN- TNF-
IL-2
Th17
Inflammation, keratinocyte hyperplasia, neovascularisation, vasodilatation, T cell/neutrophil influx
Naïve T cell
IL-23
IL-1b
DC
Th1
Naïve T cell
IL-12
DC
↑Cytokines including
IL-17 IL-22
TNF-
IL-12 and IL-23 in the development of psoriasis
Nestle FO, et al. J Invest Dermatol. 2004;123:xiv-xv.
Ustekinumab (Stelara)
PSUMMIT I
PSUMMIT II
* Early escape (EE) rules were used through Week 24; from Weeks 24- 52, EE rules were not used
PSUMMIT I and II - Ustekinumab in PsA ACR20 Responders Over Time Through Week 52*
Kavanaugh A et al. ACR, Washington, D.C., Nov 9-14, 2012. P4 Data on File
Patients with early active RA and PsA show
increased Th17 levels in peripheral blood
Leipe J, et al. Arthritis Rheum. 2010;62(10):2876-85.
Ex v
ivo
Th
17 (
%)
1.5
0.5
0
1.0
HC OA PsA RA
*
**
***
***
* p<0.05 ** p<0.01 *** p<0.001
HC: healthy control OA: osteoarthritis PsA: psoriatic arthritis RA: rheumatoid arthritis
Secukinumab - prevents IL-17A binding to its receptor
- inhibits production of pro-inflammatory mediators
IFN, interferon; IL, interleukin; TNF, tumor necrosis factor. Data on file, Novartis Pharma, AG;
Ivanov S, Linden A. Trends Pharmacol Sci. 2009;30:95–103
Secukinumab (bound to IL-17A)
IL-17A Delayed-type
hypersensitivity
and cellular
immunity
Tissue
inflammation
and pathogen
defense
Tissue
inflammation
and
pathogen
defense
IFN-γ
IL-2
IL-17F
IL-21
IL-22
IL-17A
Selective inhibition of IL-17A may be
associated with preservation of normal
components of the host immune response
Neutralization of IL-17A rapidly inhibits
downstream inflammatory cytokine and
chemokine networks and thus may be useful for
the treatment of several
immune-mediated diseases
IL-17A neutralized
by secukinumab
IL-12
Th17
Mast cells
Other leukocytes
Th1
IL-23
TNF
Dendritic
cells
Secukinumab:
fully human IgG1k
monoclonal Ab
against IL-17A
Target
tissue
cell
membrane
IL-17A
receptor
Secukinumab in PsA ACR20 Responses Through Week 52
*P < 0.0001 vs. placebo (P-values at Week 24 adjusted for multiplicity of testing).
Missing values were imputed as nonresponse (nonresponder imputation) up to Week 24. Observed data from Week 28–52.
80
0
60
40
20
0
4 24 40 52 8 12 16 20 28 32 36 44 48 1 2
69.5%
*
*
* *
* *
* *
* * * * *
66.9% 50.5%
50.0%
17.3%
Primary
endpoint
* *
*
Pe
rce
nta
ge
of R
esp
on
de
rs
Weeks
N = 202 202 202 202 202 202 202 188 183 181 181 178 179 174
N = 202 202 202 202 202 202 202 179 183 175 174 165 169 172
N = 202 202 202 202 202 202 202 -- -- -- -- -- -- --
Secukinumab 10/mg/kg i.v. 75 mg s.c. Secukinumab 10/mg/kg i.v. 150 mg s.c. Placebo
ACR50 Responses Through Week 52
*P < 0.0001, †P < 0.001; §P < 0.01; ‡P < 0.05 vs. placebo (P-values at Week 24 adjusted for multiplicity of testing)
Missing values were imputed as nonresponse (nonresponder imputation) up to Week 24. Observed data from Week 28–52.
60
0
50
40
20
0
4 24 40 52 8 12 16 20 28 32 36 44 48 1 2
30
10
* *
* *
* *
*
*
30.7%
34.7%
7.4%
* *
*
†
‡
§
50.0%
38.4%
Weeks
Pe
rce
nta
ge
of R
esp
on
de
rs
Secukinumab 10/mg/kg i.v. 75 mg s.c. Secukinumab 10/mg/kg i.v. 150 mg s.c. Placebo
N = 202 202 202 202 202 202 202 188 183 181 181 178 179 174
N = 202 202 202 202 202 202 202 179 183 175 174 165 169 172
N = 202 202 202 202 202 202 202 -- -- -- -- -- -- --
Resolution of Dactylitis and Enthesitis at
Weeks 24 and 52
*P < 0.0001 vs. placebo.
Resolution of dactylitis and enthesitis amongst those subjects with these symptoms at baseline
(dactylitis: n = 104 [150 mg], 104 [75 mg] and 116 [placebo]; enthesitis: n = 126 [150 mg], 129 [75 mg] and 117 [placebo]).
Missing values were imputed as nonresponse (nonresponder imputation) at Week 24. Observed data presented at Week 52.
Secukinumab 10 mg/kg i.v 150 mg s.c. Secukinumab 10 mg/kg i.v. 75 mg s.c. Placebo
100
Pe
rce
nta
ge
of S
ub
jects
80
60
20
0
100
Pe
rce
nta
ge
of S
ub
jects
80
60
20
0
Week 24 Week 24
40 40
Week 52 Week 52
82.6 79.4 87.7 89.7
* *
* *
Resolution of Dactylitis Resolution of Enthesitis