IMMUNOPHENOTYPING LEUKEMIAS AND
LYMPHOMASRPCI
LFC
0 256 512 768 1024
/7/06064121SSC ->
0 256 512 768 1024
/6/05133061SSC ->
CD45 Gating Strategy
NORMAL BONE MARROW LEUKEMIC (TALL) BONE MARROW
10 10 10 10 100 1 2 3 4
/6/05133061HLADr ->
10 10 10 10 100 1 2 3 4
/7/06064121HLADr ->
A B C D
erythrocytes and platelets
granulocytes monocytes and B-cells
HLADr
CD
45
HLADr
CD
45
HLADr
CD
45
A
B
C
CD45 EXPRESSION BY ACUTE B-LINEAGE LEUKEMIA
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->R5
5 6
7 810 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 4
CD14
CD45 HLADr
CD14
B LINEAGE ACUTE LEUKEMIA
HLADr
CD45
HLADr
CD45
CD45
CD14
HLADr
CD14
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 810 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 4
CD19
CD5 CD10
CD19
CD10
CD5
CD10
CD5
CD5
CD19
CD10
CD19
10 1 10 2 10 3 10 4
1
10
2
10
34
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
B LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
CD34
CD5 CD19
CD34
CD19
CD5
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->R5
5 6
7 810 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 4
CD19
CD5
CD5
CD34
CD19
CD34
B LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 810 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 4
CD11c
CD20 CD22
CD11c
CD22
CD20
CD22
CD20
CD22
CD11c
CD22
CD11c
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
B LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 810 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 4
CD13
CD33 CD19
CD13
B LINEAGE ACUTE LEUKEMIA
CD19
CD33
CD19
CD33
CD33
CD13
CD19
CD13
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
1
310 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
5
7 8
CD19
CD10
CD19
CD20
CD45
CD22
HLADr
CD34
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 410 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->R5
5 6
7 8
CD14
CD45 HLADr
CD14
HLADr
CD45
HLADr
CD45
CD45
CD14
HLADr
CD14
T LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
1-Heig
ht -->
R55 6
7 810 1 10 2 10 3 10 4
FL1-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
R11 2
3 4
CD4
CD4
T LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL1 -->
10
1
10
2
10
3
10
4
R11 2
3 410 1 10 2 10 3 10 4
FL3 -->
10
1
10
2
10
3
10
4
FL
1 --
>
5
7 810 1 10 2 10 3 10 4
FL3 -->
10
1
10
2
10
3
10
4
CD8
CD3
CD3
CD4
CD8
CD4
CD3 CD8 CD8
CD3
10 1 10 2 10 3 10 4
FL1 -->
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL3 -->
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL3 -->
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 410 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 8
CD19
CD7 CD2
CD19
CD2
CD7
CD2
CD7
CD7
CD19
CD2
CD19
T LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 410 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 8
CD19
CD5 CD10
CD19
CD10
CD5
CD10
CD5
CD5
CD19
CD10
CD19
10 1 10 2 10 3 10 4
1
10
2
10
34
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
T LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 810 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
CD34
CD5 CD19
CD34
CD19
CD5
CD19
CD5
CD5
CD34
CD19
CD34
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4T LINEAGE ACUTE LEUKEMIA
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
1-Heig
ht -->
R55 6
7 810 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
10 1 10 2 10 3 10 4
FL1-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
R11 2
3 4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
CD14
CD45 HLADr
CD14
ACUTE MYELOID LEUKEMIA
HLADr
CD45
HLADr
CD45
CD45
CD14
HLADr
CD14
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
10 1 10 2 10 3 10 4
FL1-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
R11 2
3 410 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
1-Heig
ht -->
R55 6
7 8
ACUTE MYELOID LEUKEMIA
CD33
CD11b
CD11b
CD13
CD33
CD13
CD13
CD11b CD33 CD33
CD11b
CD13
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
1-Heig
ht -->
R55 6
7 810 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
10 1 10 2 10 3 10 4
FL1-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
R11 2
3 4
10 1 10 2 10 3 10 4
FL1 -->
10 1
10 2
10 3
10 4
FL
2 -->
R11 2
3 410 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
2 -->
10 1 10 2 10 3 10 4
FL3 -->
10 1
10 2
10 3
10 4
FL
1 -->
R55 6
7 8
ACUTE MYELOID LEUKEMIA
CD56
CD15
CD15
CD34
CD56
CD34
CD34
CD15 CD56 CD56
CD15
CD34
10 1 10 2 10 3 10 4
FL1-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
R11 2
3 410 1 10 2 10 3 10 4
FL3-Height -->10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
1-Heig
ht -->
R55 6
7 8
ACUTE MYELOID LEUKEMIA
CD2
CD7
CD7
CD13
CD2
CD13
CD13
CD7 CD2 CD2
CD7
CD13
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
FL3-Height -->
10 1
10 2
10 3
10 4
FL
1-Heig
ht -->
R55 6
7 810 1 10 2 10 3 10 4
FL3-Height -->10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
10 1 10 2 10 3 10 4
FL1-Height -->
10 1
10 2
10 3
10 4
FL
2-Heig
ht -->
R11 2
3 4
ACUTE MYELOID LEUKEMIA
HLADr
CD38
CD38
CD34
HLADr
CD34
CD34
CD38 HLADr HLADr
CD38
CD34
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
10 1 10 2 10 3 10 4
10
1
10
2
10
3
10
4
8
10 1 10 2 10 3 10 4
Fluorescence One Height -->
10
1
10
2
10
3
10
4
Fluorescence Two Height -->
R1
1 2
3 410 1 10 2 10 3 10 4
Fluorescence Three Height -->
10
1
10
2
10
3
10
4
Fluorescence One Height -->
R5
5 6
7 810 1 10 2 10 3 10 4
Fluorescence One Height -->
10
1
10
2
10
3
10
4
Fluorescence Two Height -->
R1
1 2
3 4
10 1 10 2 10 3 10 4
FL1-Height -->
10
1
10
2
10
3
10
4
FL2-Height --> R1
1 2
3 410 1 10 2 10 3 10 4
FL3-Height -->
10
1
10
2
10
3
10
4
FL1-Height --> R5
5 6
7 810 1 10 2 10 3 10 4
FL1-Height -->
10
1
10
2
10
3
10
4
FL2-Height --> R1
1 2
3 4
A
D E
B
F
C
CD19
CD19
CD20
CD22
LAMBDA
LAMBDA
CD22
CD20CD5
KAPPA
KAPPA
CD5
Typical B-cell Lymphoma
10 1 10 2 10 3 10 4
Fluorescence Three Height -->
10
1
10
2
10
3
10
4
Fluorescence Two Height -->
10 1 10 2 10 3 10 4
Fluorescence One Height -->
10
1
10
2
10
3
10
4
Fluorescence Two Height -->
R1
1 2
3 410 1 10 2 10 3 10 4
Fluorescence Three Height -->
10
1
10
2
10
3
10
4
Fluorescence Two Height -->
10 1 10 2 10 3 10 4
CD19 -->
10
1
10
2
10
3
10
4
CD13 -->
10 1 10 2 10 3 10 4
FL3-Height -->
10
1
10
2
10
3
10
4
FL2-Height -->
10 1 10 2 10 3 10 4
Fluorescence One Height -->
10
1
10
2
10
3
10
4
Fluorescence Two Height -->
R1
1 2
3 410 1 10 2 10 3 10 4
FL3-Height -->
10
1
10
2
10
3
10
4
FL2-Height -->
10 1 10 2 10 3 10 4
CD19 -->
10
1
10
2
10
3
10
4
CD13 -->
A B C D
E F G H
CD19
CD19
CD19
CD13
CD19
CD13
CD19
CD19
CD2
CD2 CD7 CD10
CD7 CD10
CD19
CD19
T, CD10, and Myeloid Antigen Coexpression
in B-cell lymphoma
Lymphoma
10 1 10 2 10 3 10 4
KAPPA -->
10
1
10
2
10
3
10
4
LAMBDA -->R1
1 2
3 410 1 10 2 10 3 10 4
CD19 -->
10
1
10
2
10
3
10
4
LAMBDA -->
10 1 10 2 10 3 10 4
CD19 -->
10
1
10
2
10
3
10
4
KAPPA -->
R5
5 6
7 8
10 1 10 2 10 3 10 4
KAPPA -->
10
1
10
2
10
3
10
4
LAMBDA -->R1
1 2
3 410 1 10 2 10 3 10 4
CD19 -->
10
1
10
2
10
3
10
4
LAMBDA -->
10 1 10 2 10 3 10 4
CD19 -->
10
1
10
2
10
3
10
4
KAPPA -->
R5
5 6
7 8
CD2
CD2CD8
CD8CD3
CD3
CD7
CD7
CD4
CD4
CD4
CD4
A
FED
CB
Reactive Lymph Node
Typical T-cell Lymphoma or Reactive Lymph Node:
Problem Case
A B C
D E FKappa CD19 CD19
CD33 CD19 CD19
CD13
Kappa
Lambda
Lambda
CD33
CD13
Recommended
