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2019 WWW.CAYMANCHEM.COMCAYMAN CHEMICAL · 1180 EAST ELLSWORTH ROAD · ANN ARBOR, MI 48108 · USA · (800) 364-9897
· Assays and mass spec standards for the quantification of bioactive lipids
· Corticosteroid receptor agonists, mast cell stabilizers, and antihistamines
· Tools to study citrullination and carbamylation
· TLR and chemokine receptor modulators and NETosis assays
· Immunopeptidome profiling services
mast cell eosinophiltarget protein
PAD
citrullinatedprotein
ACPA
T cell
T cellreceptor
cytokinesCysLTsPGshistamines
DHA (DPA or EPA or AA)
cytokinesCysLTs
allergen
neutrophil
LTB4receptor
SPMs
IgE
histonesLL-37NEMPO
TLR
PAMPs
macrophageAPC
MHC
COOH
LOOK INSIDE TO LEARN MORE
· Infectious Disease · Immunosuppressants
Page 1Inflammatory Lipid Mediators
Allergy & Asthma
Autoimmunity
· Immunotherapeutics · Adaptive Immunity
Innate Immunity
Page 2
Page 3
Page 4
Page 5
Page 7
Immunology and InflammationInflammation is a critical, signal-mediated response to potential signs of danger and damage. While acute inflammation is important to the immune response, chronic, inappropriate inflammation can cause tissue destruction and lead to disease (e.g., allergies, autoimmune disorders). Cayman offers a wide range of tools to help you study the factors related to innate and adaptive immune responses.
The cellular environment during infection (or allergen exposure), immune activation, and subsequent inflammation involves a highly complex and well-coordinated team of mediators. This includes mast cells that react to danger by releasing histamines, pro-inflammatory cytokines, and eicosanoids and neutrophils/eosinophils that respond to these signals with an antimicrobial arsenal that involves NETs. Certain protein modifications, like citrullination, can alter self-epitopes and elicit an autoantibody response via T cells. Professional APCs, such as macrophages, activate T cells by displaying a MHC. SPMs, byproducts of DHA and other fatty acids, are generated later to initiate the resolution of inflammation.
1 WWW.CAYMANCHEM.COMCAYMAN CHEMICAL · 1180 EAST ELLSWORTH ROAD · ANN ARBOR, MI 48108 · USA · (800) 364-9897
Inflammatory Lipid MediatorsCayman offers a wide range of tools to study biochemical mediators released during the inflammatory process. Our core business is built around the production of bioactive lipids, including the manufacture of high-purity lipid standards and assays for their measurement, as well as biochemicals to disrupt key eicosanoid mediator production or signaling. We lead the industry as a resource for prostaglandins, leukotrienes, and specialized pro-resolving mediators pertinent to the study of immune activation and inflammation.
Leukotrienes
Prostaglandins
Specialized Pro-Resolving MediatorsItem No. Product Name
20110 Leukotriene B4
520111 Leukotriene B4 ELISA Kit*
120114 BLT1 Receptor Polyclonal Antibody
20210 Leukotriene C4
20310 Leukotriene D4
20410 Leukotriene E4
501060 Leukotriene E4 ELISA Kit
500390 Cysteinyl Leukotriene ELISA Kit*
20001 Cysteinyl Leukotriene HPLC Mixture I
20002 Cysteinyl Leukotriene HPLC Mixture II
120500 CysLT1 Receptor Polyclonal Antibody
14713 CysLT1 Receptor Polyclonal FITC Antibody
Item No. Product Name
501001 tetranor-PGDM ELISA Kit
12010 Prostaglandin D2
512031 Prostaglandin D2 ELISA Kit*
512011 Prostaglandin D2-MOX ELISA Kit*
14010 Prostaglandin E2
314010 Prostaglandin E2-d4
514010 Prostaglandin E2 ELISA Kit - Monoclonal*
516521 11β-Prostaglandin F2α ELISA Kit
*Also available: express kits
Item No. Product Name
90410 Lipoxin A4
590410 Lipoxin A4 ELISA Kit
90415 15(R)-Lipoxin A4
90420 Lipoxin B4
10878 Maresin 1
501150 Maresin 1 ELISA Kit
16369 Maresin 2
17007 MCTR1
17008 MCTR2
19067 MCTR3
19064 PCTR1
10010390 Protectin D1
10012554 Resolvin D1
13060 17(R)-Resolvin D1
500380 Resolvin D1 ELISA Kit
10007279 Resolvin D2
501120 Resolvin D2 ELISA Kit
13834 Resolvin D3
13835 Resolvin D4
10007280 Resolvin D5
18702 SPM D-series LC-MS Mixture
10007848 Resolvin E1
Common SPMs listed; methyl ester forms, internal standards, and LC-MS mixtures also available
SPECIALIZED PRO-RESOLVING MEDIATORS The resolution of inflammation is an active process that is driven by unique signaling molecules. An important group of these specialized pro-resolving mediators (SPMs) is derived from polyunsaturated fatty acids (arachidonic acid (AA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA)) that are liberated during the inflammatory process. Studies have demonstrated that these SPMs, which include resolvins, lipoxins, maresins, and protectins, evoke cellular and tissue responses that help conclude an inflammatory episode. Moreover, the addition of nanomolar concentrations of SPMs to an inflammatory milieu can have profound effects, such as reducing leukocyte infiltration, enhancing phagocytosis, and reducing pain. Cayman Chemical, a leader in the synthesis of lipid mediators, offers an array of SPMs to help make your research possible.
www.caymanchem.com
COOH
DHA (90310)
13(S),14(S)-epoxy-MaresinCOOH
O
Maresin 2 (16369)
COOH
OH
HO
14(S)-HpDHACOOH
OOH
15-LO
15-LO12-LO
SolubleEpoxide
Hydrolase
Maresin 1 (10878)
COOH
OH
OH
Hydrolase 14(S)-HDHA (15253)
COOH
OH
14(S),21(R)-HDHA
COOH
OHOH
17(S)-HpDHA (13185)
COOH
HOO
Protectin D1/Neuroprotectin D1 (10010390)
OH
OH
COOH
10(S),17(S)-DiHDHA (PDX) (10008128)
OH
COOH
OH
16(S),17(S)-epoxy Protectin
COOHO
15-LO
5-LO
17(S)-HpDHA (13185)
COOH
HOO
Resolvin D1 (10012554)(17(R)-Resolvin D1, 13060)
HO
COOH
HO
OH
Resolvin D2 (10007279)
COOH
HO
HO
OH
Resolvin D5 (10007280)
COOH
HO
HO
7(S)-hydroperoxy-17(S)-HDHAHO
COOH
HOO
Resolvin D3 (13834)
COOH
OH
OH
HO
Resolvin D6 (10484)
HOOC
OH
OH
4(S)-hydroperoxy-17(S)-HDHA
HO
COOHHOO
7(S),8(S)-epoxy-17(S)-HDHAHO
COOH
O
4(S),5(S)-epoxy- 17(S)-HDHA
HO
COOH
O
5-LO
5-LO
Resolvin D4 (13835)
COOH
HO
HO
OH
14(S),20(R)-DiHDHA (19139)
COOH
OH
HO
D-Resolvin Family
Protectin Family
Maresin Family
PCTR2 (19065)
COOH
S
OH
H2N
NH
O
COOH
PCTR3 (19066)
COOH
S
OH
H2N
HOOC
MCTR1 (17007)
COOH
OH
S
NH
O
HOOC
NH2HN
O
HOOC
MCTR2 (17008)COOH
OH
S
NH2HN
O
HOOC
MCTR3 (19067)COOH
OH
SHOOC
NH2
COOHS
OH
NH
O
H2N
COOH NH
O
COOH
PCTR1 (19064)
COOH
OH
OH
OH
COOH
Resolvin T1 (18437)
OH
OH
OH
COOH
Resolvin T2 (18438)
OH
OH
OH
COOH
Resolvin T3 (18439)
OH OH
COOH
Resolvin T4 (18440)
DPA (90165)
T-Resolvin Family
COX-2
COOH
HOO 13(R)-HpDPA
COOH
HO 13(R)-HDPA
E-Resolvin Family
COOH
EPA (90110)P450 Aspirin & COX-2
HOCOOHOH
OH
Resolvin E1 (10007848)
OH
COOH
OH
Resolvin E2 (13827)
COOH
OHHO Resolvin E3
COOH
OOH18(R)-HpEPE
COOH
OH18(R)-HEPE
OH
COOH
OOH
5(S)-hydroperoxy-18(R)-HEPE
OH
COOHO
5(S),6(S)-epoxy-18(R)-HEPE
5-LO
Lipoxin Family
COOH
AA (90010)
15-LO 5-LO
5-LO
Lipoxin A4 (90410)(15(R)-Lipoxin A4, 90415)
OH
COOH
OHHO
*5(S)-HpETE (44230)
COOH
OOH
*15(S)-HpETE (44720)
COOH
OOH
*5(S),15(S)-DiHpETE
COOH
OOH
OOH
COOHO
HO
*5(S),6(S)-epoxy-15(S)-DiHETE
Lipoxin B4 (90420)
COOH
OH
OHHO
*Have not been shown to have pro-resolving properties in published literature
Request the full Specialized Pro-Resolving Mediators wall poster at www.caymanchem.com/Literature
2WWW.CAYMANCHEM.COM CAYMAN CHEMICAL · 1180 EAST ELLSWORTH ROAD · ANN ARBOR, MI 48108 · USA · (800) 364-9897
Allergy & AsthmaImmune activation and inflammation are both characteristics of an allergic or asthmatic response. Cayman’s line of allergy and asthma research products includes tools for measuring and disrupting eicosanoid mediators and assays to assess ovalbumin-induced immune responses. Key corticosteroid receptor agonists, mast cell stabilizers, histamine receptor antagonists/inverse agonists, phosphodiesterase inhibitors, and β-adrenergic receptor agonists are also available.
Dihydroxy metabolites of linoleic acid may be biomarkers for the development of asthma and important mediators of fatty acid metabolism. Cayman offers competitive ELISAs to quantify 9(10)-DiHOME and 12(13)-DiHOME in plasma and serum.
Over 10 corticosteroid receptor agonists available online
Over 30 histamine receptor antagonists and/or inverse agonists available online
Over 10 β-adrenergic receptor agonists available online
Over 10 PDE inhibitors as well as related adenosine receptor antagonists available online
Corticosteroid Receptor Agonists
Ovalbumin Assay Kits
Antihistamines
Mast Cell Stabilizers
Phosphodiesterase Inhibitors
β-Adrenergic Receptor Agonists
Item No. Product Name
15407 Budesonide
20739 Hydrocortisone
18026 Triamcinolone acetonide
Item No. Product Name
500840 Anti-Ovalbumin IgE (mouse) ELISA Kit
500830 Anti-Ovalbumin IgG1 (mouse) ELISA Kit
Item No. Product Name
16967 Astemizole
14637 Clemastine (fumarate)
15625 Loratadine
14615 Meclizine (hydrochloride)Item No. Product Name
22173 Compound 48/80 (hydrochloride)
21379 Cromolyn (sodium salt hydrate)
23994 Lodoxamide
Item No. Product Name
14832 Ibudilast
15141 Roflumilast
10011132 Rolipram
10008671 Sildenafil
Item No. Product Name
14985 Clenbuterol (hydrochloride)
15584 Formoterol (hemifumarate)
16009 Salmeterol (xinafoate)
(±)12(13)-DiHOME ELISA Kit Item No. 501720
(±)9(10)-DiHOME ELISA Kit Item No. 501710
Histamine EIA Kit - Item No. 589651Conveniently Detect Histamine
· Measure histamine in plasma, serum, bronchoalveolar lavage, tissue extracts, and cell culture supernatant samples
· Assay 33 samples in duplicate · Incubation: Overnight
Corresponding unlabeled and deuterated standards for EpOMEs and DiHOMEs are also available for quantification by mass spec
DiHOMEs: Potential drivers of allergic responses and thermogenic activity
Manufactured by Bertin Bioreagent
3 WWW.CAYMANCHEM.COMCAYMAN CHEMICAL · 1180 EAST ELLSWORTH ROAD · ANN ARBOR, MI 48108 · USA · (800) 364-9897
AutoimmunityAutoimmune disorders, such as rheumatoid arthritis and systemic lupus erythematosus, are tied to post-translational modifications of proteins, specifically citrullination and carbamylation. Cayman offers a unique collection of tools to study citrullination and carbamylation. This includes citrullinated histones and citrullinated human fibrinogen as well as key detection antibodies. Protein arginine deiminase (PAD) ELISAs, recombinant proteins, antibodies, and inhibitors are also available.
Anti-Carbamylation (Homocitrulline) Polyclonal Antibody - Item No. 22428Specifically detects carbamylated proteins of any type and from any species
A set of assays to enable the screening of large libraries of compounds for the next generation of PAD inhibitors
Also available: Anti-Carbamylation (Homocitrulline) Monoclonal Antibody (Item No. 23203) and carbamylation reagents
Also available: Assay kits for PAD1, 2, 3, and 4 in ammonia and AMC formats
Detect Carbamylated Proteins
Screening Assays for Identifying Inhibitors of PAD Activity
Citrullinated Histones and Related Reagents
PAD Inhibitors PAD ELISAs
PAD Monoclonal Antibodies
Citrullinated Fibrinogen and Related ReagentsItem No. Product Name
20582 Citrullinated Core Histones (bovine)
17926 Citrullinated Histone H3 (human recombinant)
501620 Citrullinated Histone H3 (Clone 11D3) ELISA Kit
18073 Histone H1.4 (Citrullinated R53) Polyclonal Antibody
17939 Histone H3 (Citrullinated R2 + R8 + R17) Monoclonal Antibody
17855 Histone H3 (Citrullinated R2 + R8 + R17) Polyclonal Antibody
Item No. Product Name
17079 BB-Cl-Amidine
17489 GSK199 (hydrochloride)
17488 GSK484 (hydrochloride)
17731 YW3-56 (hydrochloride, technical grade)
Item No. Product Name
501450 PAD2 (human) ELISA Kit
501460 PAD4 (human) ELISA Kit
500930 PAD4 Autoantibody ELISA Kit
Recombinant PAD ProteinsItem No. Product Name
10784 PAD1 (human recombinant)
10785 PAD2 (human recombinant)
10786 PAD3 (human recombinant)
10500 PAD4 (human recombinant)
Item No. Product Name
22997 PAD1 Monoclonal Antibody (Clone 6B4)
19822 PAD2 Monoclonal Antibody (Clone 9F7)
24377 PAD3 Monoclonal Antibody (Clone 4E5)
19671 PAD4 Monoclonal Antibody (Clone 11F9)
19669 PAD4 Monoclonal Antibody (Clone 6D8)
Item No. Product Name
18473 Human Fibrinogen (PAD2 Citrullinated)
400076 Human Fibrinogen (PAD4 Citrullinated)
501270 Anti-Citrullinated Human Fibrinogen Assay Kit (mouse)
23000 Citrullinated α-Enolase Monoclonal Antibody (Clone 8D3)
17088 Citrullinated Fibrinogen Monoclonal Antibody (Clone 10E9.3)
22054 Citrullinated Vimentin Monoclonal Antibody (Clone 12G11)
4WWW.CAYMANCHEM.COM CAYMAN CHEMICAL · 1180 EAST ELLSWORTH ROAD · ANN ARBOR, MI 48108 · USA · (800) 364-9897
Immunotherapeutics
Adaptive Immunity
Cayman offers Human Therapeutic IgG1, IgG2, and IgG4 ELISA Kits for use in accurately measuring human IgG species in monkey or rodent plasma and serum. These assays can help distinguish experimentally introduced human IgG from endogenous monkey IgG when assessing the pharmacokinetics of human therapeutic antibodies in monkey serum.
Cayman, in partnership with MS Bioworks, has optimized a workflow to enable MHC Class I and MHC Class II Associated Peptide Proteomics (MAPPs). Our collective expertise provides efficient, cost-effective deepsequence analysis of immunogenic peptides from tumor cells and biological therapeutics.
Human Therapeutic IgG1 ELISA Kit - Item No. 500910 Assay Range: 3.13-200 ng/mlLLOQ = 3.13 ng/ml
Human Therapeutic IgG2 ELISA Kit - Item No. 500960 Assay Range: 3.13-200 ng/mlLLOQ = 3.13 ng/ml
Human Therapeutic IgG4 ELISA Kit - Item No. 500920 Assay Range: 7.8-500 ng/mlLLOQ = 7.8 ng/ml
Validated ELISA kits for pharmacokinetic analysis of therapeutic human antibodies
Immunopeptidome Profiling Services
· Specifically detect human IgG1, IgG2, or IgG4 antibodies in monkey plasma or serum without prior sample purification
· Not cross reactive to rhesus or cynomolgus immunoglobulins
· Pharmacokinetic analysis of therapeutic human IgG1, IgG2, or IgG4 antibodies in monkeys
· Detect human IgG1, IgG2, or IgG4 in mouse or rat plasma or serum
· Identification of thousands of MHC-associated peptides
· Allele-specific studies matched to your specifications
· Analysis using Orbitrap MS and unbiased, deep sequencing of the MHC peptidome
· Customized antibody production, cell culture, and bioanalysis
CELLS OR TISSUE
LYSIS
MHC IP PEPTIDE ELUTION ORBITRAP LC-MS/MS DATA ANALYSIS
MHC Class I MHC Class II
m/z
MHCPeptides
5 WWW.CAYMANCHEM.COMCAYMAN CHEMICAL · 1180 EAST ELLSWORTH ROAD · ANN ARBOR, MI 48108 · USA · (800) 364-9897
Innate ImmunityInnate immunity is a key defense system activated in response to bacterial and viral infections. Cayman offers a collection of tools to study host defense mechanisms that includes chemokine receptor and toll-like receptor modulators, phagocytosis and neutrophil extracellular trap assays, and proteins involved in STING signaling and the induction of the type 1 interferon (IFN) response.
Chemokine Receptor Modulators Phagocytosis Assay Kits
CD36 Scavenger Receptor Antibodies
Item No. Product Name
27633 AZD 2098
11479 Bindarit
28421 BMS 813160
18503 BX 471
21885 CCR4 Antagonist (hydrochloride)
23927 Cenicriviroc
28433 INCB 3344
14641 Maraviroc
21803 MK-0812
21492 Reparixin (L-Lysine salt)
13336 SB 225002
11974 TC 14012 (trifluoroacetate salt)
Item No. Product Name
500290 Phagocytosis Assay Kit (IgG FITC)
601480 Phagocytosis Assay Kit (IgG-DyLight™ 405)
601490 Phagocytosis Assay Kit (IgG-DyLight™ 633)
600540 Phagocytosis Assay Kit (IgG PE)
601370 E. coli Phagocytosis Assay Kit
Item No. Product Name
188150 CD36 Monoclonal Antibody (Clone JC63.1)
10009893 CD36 Monoclonal Antibody (Clone JC63.1) (azide free)
10009870 CD36 Monoclonal FITC Antibody (Clone JC63.1)
100011 CD36 Polyclonal Antibody
Over 20 chemokine receptor agonists, antagonists, and inverse agonists available online
Neutrophil Extracellular Traps (NETs) release DNA, histones, and other antimicrobial components extracellularly to capture and kill pathogens. This may be initiated by respiratory burst, MPO-induced chromatin decondensation, or histone deimination. If not cleared properly, NETs can be a target of autoantibody formation.
Antibodies Assay KitsItem No. Product Name
15635 dsDNA Monoclonal Antibody (Clone 2C4)
13784 Histone H3.3 Polyclonal Antibody (Also available: 13535, 13543)
15637 LL-37 Polyclonal Antibody
15641 Lysozyme C Monoclonal Antibody (Clone 4C3)
20493 Myeloperoxidase (mouse) Polyclonal Antibody (Also available: 15638, 15640)
Item No. Product Name
501410 Myeloperoxidase (human) ELISA Kit
601010 NETosis Assay Kit
600610 Neutrophil Elastase Activity Assay Kit
600620 Neutrophil Myeloperoxidase Activity Assay Kit
601130 Neutrophil/Monocyte Respiratory Burst Assay Kit
601070 Neutrophil (mouse) Isolation Kit
Granule Contents
Components Expelled During NETosis
Triggers of NET Formation
NEUTROPHIL BIOLOGY
Macrophage
Serum Nucleases
Macrophage
CD4+ T cell
B cell
IL-18
Th1/Th17 cytokines
ANCAACPA
Anti-dsDNA
Autoantigen immunecomplexes
Priming andreduced activation
threshold
Type Iinterferons
Pro-inflammatorycytokines
IL-1β
T cell
PlasmacytoidDendritic Cell
PADactivation MPO
NE
MyeloidDendritic Cell
Complement
Complement receptors
Fc receptors
MPO MPO
NE
Ca2+
Ca2+
Ca2+ NEcit cit
MPO
TLR
TLR4
IL receptor
Integrins
Antibodies
ActivatedPlatelets
PMA
Bacteria
Cytokines
Parasites
Viruses
Vital NETosis:· PAD activation· Chromatin decondensation· NETs expelled via vesicles· Neutrophil remains alive as functional anuclear cytoplast
· Calgranulin· Cathepsin G· Defensins· Elastase (NE)· Lactoferrin· Leukocyte proteinase (PR3)· LL-37· Myeloperoxidase (MPO)
· Azurocidin· Calprotectin· Cathelicidins· Cathepsin G· Defensins· Elastase (NE)· Gelatinase· Genomic DNA
· Activated platelets· Autoantibodies· Bacteria· Cholesterol crystals· Cigarette smoke· Complement-derived peptides· Endotoxins (bacterial component, LPS)· Fungi
Neutrophil Phagocytosis
Internalized microbes are destroyed by the respiratory burst (ROS produced by NADPH oxidase) and by fusion of the phagosome with granules. This process can result in neutrophil apoptosis, resolving inflammation, or necrosis, promoting further inflammation, depending on the pathogen ingested.
Lytic versus Vital NETosisLytic NETosis:· NADPH activation/ROS· PAD activation· Nuclear envelope disassembly· Chromatin decondensation
· Plasma membrane rupture· NETs released· Neutrophil dies
DirectStimulation
Self-AntigenPresentation
Degradation & Removal
ROS ROS
· Hydrogen peroxide (substrate of MPO)· Interleukin-8 (IL-8)· Ionophores (A23187, Nigericin)· Lipid mediators (2-chloro fatty acid [2-CIFA])· Protozoa· Urate crystals· Viruses
O
OO
O
H
OH
HO
OH
O
HH
PAD Modifies Arginine to Citrulline
Ca2+
H2O
NH4+
PAD
H2N+
NH2
NH
Protein
H2N O
NH
Protein
Peptidyl Arginine(+ charge)
Peptidyl Citrulline(neutral)
Citrullination Targets:· β-Catenin· Cytokeratin· α-Enolase· Fibrinogen· Filaggrin· Heat shock proteins· Histones· Vimentin· and more!
Phagosome
MicrobeSpecificgranules
Azurophilicgranules
ROS
ROS
PAD Feedback Loop HaltsRespiratory Burst
NADPHoxidase
NADPHoxidase
ROS
Activated NADPH oxidase associated with PAD produces ROS
NCF4
NCF1
NCF2
PAD
Calcium influx activates PAD to citrullinate NCF proteins, which dissociate and inhibit ROS
Rac
cit
cit
Ca2+
NCF4NCF1
NCF2
PAD
Rac
© 2019 Cayman ChemicalSpecial thanks to Dr. Cameron Louttit from the University of Michigan Department of Biomedical Engineering for consultation on this diagram.
· Histones· Lactoferrin· Leukocyte proteinase (PR3)· Lysozyme C· Mitochondrial DNA· Myeloperoxidase (MPO)
NET Involvement in Sepsis
Bacterial sequestration
Thrombosis
Organdamage
Endothelialdamage
Rheumatoid Arthritis (RA) Progression
NET-Associated Thrombosis
NET-Assisted Cancer Metastasis
Anti-Inflammatory Role ofAggregated NETs
Pro-inflammatorycytokines
Sequestered andproteolytically degraded
cytokinesAggregated NETs
Platelet activation Thrombin activation Fibrin clot formation Deposition & damage
Tissue factor
Citrullination
Citrullinated humanjoint proteins
Autoantigen immunecomplexes
Perpetuationof inflammation
leading tochronic RA
Jointinflammation
Cancer-associatedthrombosis
G-CSF
Stromal weakeningand degradation
Systemic/chronicinflammation
Disseminatedcancer cell
NETs trap cancer cellsand assist in extravasation
PAD
NADPH oxidase
Request a copy of our Neutrophil Biology wall poster for your lab at www.caymanchem.com/literature
6WWW.CAYMANCHEM.COM CAYMAN CHEMICAL · 1180 EAST ELLSWORTH ROAD · ANN ARBOR, MI 48108 · USA · (800) 364-9897
STING and Interferon Induction
STING dimer (A and B are both aa 139-379) bound to cyclic di-GMP and notated with key amino acid modifications of Cayman's STING variants.
Item No. Product Name
22816 STING R232 variant (human recombinant)
22815 STING H232 variant (human recombinant)
15139 STING H232 variant; SUMO-tagged (human recombinant)
23592 STING AQ variant (human recombinant)
23594 STING M284 variant (human recombinant)
23593 STING R224 variant (human recombinant)
25306 STING A162 variant (human recombinant)
Item No. Product Name
22810 cGAS (human recombinant)
25001 cGAS (161-522) (human recombinant)
22809 CdnP (Mycobacterium tuberculosis strain ATCC 25618/H37Rv recombinant)
22817 TBK1 (human recombinant)
22811 IRF3 (human recombinant)
23590 IRF3 (S386A, S396A mutant; human recombinant)
Item No. Product Name
23853 cGAS Monoclonal Antibody (Clone 5G10)
17856 STING Monoclonal Antibody (Clone 2C8)
17857 STING Polyclonal Antibody
24791 STING M284 variant Polyclonal Antibody
24937 IRF3 Polyclonal Antibody
13931 IKKγ Monoclonal Antibody (Clone 72C627)
STING Variants
Upstream/Downstream Proteins in the STING Pathway cGAS, STING, IRF3, and IKKγ Antibodies
Read about how amino acid variations affect innate immune response to foreign DNA at www.caymanchem.com/STINGvariants
Antibodies Agonists and Pathogen-Associated Molecular PatternsItem No. Product Name
13582 Toll-Like Receptor 1 Polyclonal Antibody
13587 Toll-Like Receptor 2 Monoclonal Antibody (Clone TL2.1)
13589 Toll-Like Receptor 4 Monoclonal Antibody (Clone HTA125)
13593 Toll-Like Receptor 9 Monoclonal Antibody (Clone 26C593.2)
Item No. Product Name
14956 Imiquimod
19661 LPS from Escherichia coli O111:B4
24126 Pam3CSK4 (trifluoroacetate salt)
14806 R-848
Additional agonists and inhibitors are available onlineAdditional antibodies are available online
Toll-Like Receptors
2'3'-cGAMP ELISA Kit - Item No. 501700 · Measure 2'3'-cGAMP in cell lysates down to 85.3 pg/ml · Monitor the kinetics of 2'3'-cGAMP formation and hydrolysis · Identify compounds that modulate cGAS activity by monitoring cGAMP levels
Powered by
Monitor second messenger 2’3’-cGAMP in cells
2019 WWW.CAYMANCHEM.COMCAYMAN CHEMICAL · 1180 EAST ELLSWORTH ROAD · ANN ARBOR, MI 48108 · USA · (800) 364-9897
Infectious Disease
Immunosuppressants
Cayman provides a diverse set of antimicrobial and antiviral agents, many of which are derived from natural resources, that can be used to study the treatment,prevention, and/or antibiotic resistance of infectious diseases and other health challenges. This important set of tools can be used to further understand the growth cycle of harmful microbes and key signaling pathways, including how the body's immune response system works to eliminate infections.
Immunosuppressive agents are designed to limit the production of inflammatory mediators, disrupt the activation of T cells, prevent cell proliferation, and/or impair the function of cell surface markers, thus inhibiting stimulation of T cells directed against foreign antigens. Cayman offers a targeted set of compounds that can be used to study these various therapeutic strategies for application in organ transplant survival as well as diseases with an inflammatory component.
Item No. Product Name
15305 A-54556A
14417 Ampicillin (sodium salt)
15004 Azithromycin
14422 Doxycycline (hyclate)
16488 Monensin (sodium salt)
20766 Piperacillin (sodium salt)
14157 Polymyxin B (sulfate)
17185 Tazobactam (sodium salt)
15327 Vancomycin (hydrochloride)
Item No. Product Name
21065 N-Butyldeoxynojirimycin (hydrochloride)
22191 Dolutegravir
10009197 Flavopiridol (hydrochloride)
11208 KRN 7000
14641 Maraviroc
15144 Nelfinavir (mesylate)
10011332 Plerixafor (hydrochloride hydrate)
22411 Procyanidin B1
11974 TC 14012 (trifluoroacetate salt)
Item No. Product Name
15316 Auranofin
12088 Cyclosporin A
10007965 FK-506
63150 Myriocin
13346 Rapamycin
10007974 SLF
Antibiotics Antivirals
Potent Immunosuppressants
Common antibiotics listed, over 655 antibiotics available online Common antivirals listed, over 320 available online
Common immunosuppressants listed, 40 available online
Contact UsPHONE EMAIL
ADDRESS
FAXWEB
(800) 364-9897 (USA and Canada only) (734) 971-3335
Sales: [email protected] Customer Service: [email protected] Technical Support: [email protected] Contract Services: [email protected]
1180 E. Ellsworth Rd. · Ann Arbor, MI · 48108 · USA
(734) 971-3640
www.caymanchem.com
In Italy: Vinci-Biochem SrlContatto diretto: tel. 0571 568 [email protected]
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