FRAENKEL - CONRAT S EXPERIMENT

FRAENKEL - CONRAT’S

EXPERIMENT

(DNA as the carrier of genetic

information)

COMPILED AND CIRCULATED BY DR. UTTAM KUMAR KANP, ASSISTANT PROFESSOR,

DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE

BOTANY: SEM-IV, PAPER-C8T: MOLECULAR BIOLOGY, UNIT-1: NUCLEIC ACIDS: CARRIERS OF GENETIC INFORMATION .

DR. UTTAM KUMAR KANP

ASSISTANT PROFESSOR

DEPARTMENT OF BOTANY

NARAJOLE RAJ COLLEGE

DNA is deoxyribo nucleic acid.

DNA contain three main components (1) phosphate groups (2)

five –carbon sugars; and (3) nitrogen – containing bases called

purines [Adenine (A) and guanine (G)] and pyrimidines [

thymine (T) and cytosine (C) ]. Nitrogen base and sugar make a

nucleoside. Phosphate group and a nucleoside make nucleotide.

DNA is composed of two stand of nucleotides. The two strands

are held together by H- bonds .(complementary base pairing)

A pyrimidine in one chain is always paired with a purine in the

other chain.













HERSHEY AND

CHASE

GRIFFITH













FRAENKEL-CONRAT’S EXPERIMENT They were working with TMV in 1957 to prove that RNA can

act as genetic material.

TMV has a protein capsid and RNA inside.

They separated the protein coat and the RNA and used themto infect tobacco leaves.

When only the separated protein coat was used, no infectionoccurred while only RNA could infect tobacco leaves.

When the separated protein coat and RNA wererecombined ,entire TMV particles were recommitted.




•Next , they selected two strains of TMV;TMV-A

(causes mottling of leaves ) and TMV-B (causes wrinkle pattern on leaves),

•The protein and RNA parts of each strain were separated and chimeric TMV

particles were generated.

•The chimeric TMV produced symptoms that were characteristics of the RNA

and not the protein.




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FRAENKEL - CONRAT S EXPERIMENT